nebnext end repair module  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext end repair module
    Nebnext End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext end repair module/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nebnext end repair module - by Bioz Stars, 2020-05
    93/100 stars

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    Amplification:

    Article Title: Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris
    Article Snippet: .. After sonication, the genomic fragments were end repaired using the NEBNext End Repair module (New England Biolabs) in order to effectively ligate on adaptors for amplification and sequencing, and A-tails were added by incubation with Taq polymerase with 0.2 mM dATP at 72 °C for 30 minutes to allow the ligation of T-tailed adapters. .. T-tailed adapter was ligated to the A-tailed fragments with T4 DNA ligase (New England Biolabs) overnight at 16 °C.

    Article Title: Multiplexed Nanopore Sequencing of HLA-B Locus in Māori and Pacific Island Samples
    Article Snippet: .. Briefly, 5 μg of purified amplicon library was prepared with the NEBNext end repair module (New England Biolabs), then dA-tailed using the NEBNext dA-tailing module (New England Biolabs). .. Briefly, 5 μg of purified amplicon library was prepared with the NEBNext end repair module (New England Biolabs), then dA-tailed using the NEBNext dA-tailing module (New England Biolabs).

    Ligation:

    Article Title: Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris
    Article Snippet: .. After sonication, the genomic fragments were end repaired using the NEBNext End Repair module (New England Biolabs) in order to effectively ligate on adaptors for amplification and sequencing, and A-tails were added by incubation with Taq polymerase with 0.2 mM dATP at 72 °C for 30 minutes to allow the ligation of T-tailed adapters. .. T-tailed adapter was ligated to the A-tailed fragments with T4 DNA ligase (New England Biolabs) overnight at 16 °C.

    Purification:

    Article Title: Multiplexed Nanopore Sequencing of HLA-B Locus in Māori and Pacific Island Samples
    Article Snippet: .. Briefly, 5 μg of purified amplicon library was prepared with the NEBNext end repair module (New England Biolabs), then dA-tailed using the NEBNext dA-tailing module (New England Biolabs). .. Briefly, 5 μg of purified amplicon library was prepared with the NEBNext end repair module (New England Biolabs), then dA-tailed using the NEBNext dA-tailing module (New England Biolabs).

    Sequencing:

    Article Title: Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris
    Article Snippet: .. After sonication, the genomic fragments were end repaired using the NEBNext End Repair module (New England Biolabs) in order to effectively ligate on adaptors for amplification and sequencing, and A-tails were added by incubation with Taq polymerase with 0.2 mM dATP at 72 °C for 30 minutes to allow the ligation of T-tailed adapters. .. T-tailed adapter was ligated to the A-tailed fragments with T4 DNA ligase (New England Biolabs) overnight at 16 °C.

    Article Title: Draft Genome Sequence of the Strawberry Anthracnose Pathogen Colletotrichum fructicola
    Article Snippet: .. Paired-end PCR-free genomic libraries were prepared for Illumina sequencing using New England Biolabs Next End Repair (catalog number E6050S), dA-tailing (catalog number E6053S), and Blunt T/A ligase (catalog number M0367S) module reagents. .. For MinION sequencing, library preparation was performed using a 1D genomic DNA ligation sequencing kit (catalog number SQK-LSK108; Oxford Nanopore Technologies), with shearing performed using a g-TUBE (Covaris) and size selection on a BluePippin system ( > 4 kbp).

    Incubation:

    Article Title: Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris
    Article Snippet: .. After sonication, the genomic fragments were end repaired using the NEBNext End Repair module (New England Biolabs) in order to effectively ligate on adaptors for amplification and sequencing, and A-tails were added by incubation with Taq polymerase with 0.2 mM dATP at 72 °C for 30 minutes to allow the ligation of T-tailed adapters. .. T-tailed adapter was ligated to the A-tailed fragments with T4 DNA ligase (New England Biolabs) overnight at 16 °C.

    Polymerase Chain Reaction:

    Article Title: Draft Genome Sequence of the Strawberry Anthracnose Pathogen Colletotrichum fructicola
    Article Snippet: .. Paired-end PCR-free genomic libraries were prepared for Illumina sequencing using New England Biolabs Next End Repair (catalog number E6050S), dA-tailing (catalog number E6053S), and Blunt T/A ligase (catalog number M0367S) module reagents. .. For MinION sequencing, library preparation was performed using a 1D genomic DNA ligation sequencing kit (catalog number SQK-LSK108; Oxford Nanopore Technologies), with shearing performed using a g-TUBE (Covaris) and size selection on a BluePippin system ( > 4 kbp).

    Sonication:

    Article Title: Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris
    Article Snippet: .. After sonication, the genomic fragments were end repaired using the NEBNext End Repair module (New England Biolabs) in order to effectively ligate on adaptors for amplification and sequencing, and A-tails were added by incubation with Taq polymerase with 0.2 mM dATP at 72 °C for 30 minutes to allow the ligation of T-tailed adapters. .. T-tailed adapter was ligated to the A-tailed fragments with T4 DNA ligase (New England Biolabs) overnight at 16 °C.

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    New England Biolabs nebnext end repair module
    Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by <t>NEBNext</t> dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres
    Nebnext End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext end repair module/product/New England Biolabs
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nebnext end repair module - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    94
    New England Biolabs nebnext ultra ii end repair da tailing module
    Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by <t>NEBNext</t> dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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    Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by NEBNext dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres

    Journal: Genetics

    Article Title: Stable Patterns of CENH3 Occupancy Through Maize Lineages Containing Genetically Similar Centromeres

    doi: 10.1534/genetics.115.177360

    Figure Lengend Snippet: Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by NEBNext dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres

    Article Snippet: We end-repaired and 5′-phosphorylated using the End Repair Module (New England Biolabs #E6050L), A-tailed with Klenow exo- (New England Biolabs #M0212S), and ligated to adapters (8.3 nM) with a Quick Ligase Kit (New England Biolabs #M2200S).

    Techniques: Produced