5 alpha f iq e coli  (New England Biolabs)


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    New England Biolabs 5 alpha f iq e coli
    5 Alpha F Iq E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    5 alpha f iq e coli - by Bioz Stars, 2022-08
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    New England Biolabs dh5 alpha escherichia coli
    Sanger sequencing of partial genomic DNA flanking the putative heterozygous SNP marker 485_1_1286 as called by SAMtools mpileup and bcftools with default parameters from M. roreri isolates C13. PCR-amplicons were cloned into pCR4-TOPO vector followed by transformation into <t>DH5-alpha</t> <t>Escherichia</t> coli and 9–11 clones from each amplicon were randomly selected to be sequenced from both ends using M13F and M13R primers. ClustalW2 ( Larkin et al., 2007 ) was used to compare the DNA sequences of each clone and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program ( http://phylogeny.lirmm.fr/ ) and the neighbor-joining method.
    Dh5 Alpha Escherichia Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh5 alpha escherichia coli/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dh5 alpha escherichia coli - by Bioz Stars, 2022-08
    99/100 stars
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    99
    New England Biolabs 5 alpha f iq e coli
    Sanger sequencing of partial genomic DNA flanking the putative heterozygous SNP marker 485_1_1286 as called by SAMtools mpileup and bcftools with default parameters from M. roreri isolates C13. PCR-amplicons were cloned into pCR4-TOPO vector followed by transformation into <t>DH5-alpha</t> <t>Escherichia</t> coli and 9–11 clones from each amplicon were randomly selected to be sequenced from both ends using M13F and M13R primers. ClustalW2 ( Larkin et al., 2007 ) was used to compare the DNA sequences of each clone and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program ( http://phylogeny.lirmm.fr/ ) and the neighbor-joining method.
    5 Alpha F Iq E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 alpha f iq e coli/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 alpha f iq e coli - by Bioz Stars, 2022-08
    99/100 stars
      Buy from Supplier

    97
    New England Biolabs neb 5 alpha competent e coli high efficiency
    Sanger sequencing of partial genomic DNA flanking the putative heterozygous SNP marker 485_1_1286 as called by SAMtools mpileup and bcftools with default parameters from M. roreri isolates C13. PCR-amplicons were cloned into pCR4-TOPO vector followed by transformation into <t>DH5-alpha</t> <t>Escherichia</t> coli and 9–11 clones from each amplicon were randomly selected to be sequenced from both ends using M13F and M13R primers. ClustalW2 ( Larkin et al., 2007 ) was used to compare the DNA sequences of each clone and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program ( http://phylogeny.lirmm.fr/ ) and the neighbor-joining method.
    Neb 5 Alpha Competent E Coli High Efficiency, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb 5 alpha competent e coli high efficiency/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb 5 alpha competent e coli high efficiency - by Bioz Stars, 2022-08
    97/100 stars
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    Sanger sequencing of partial genomic DNA flanking the putative heterozygous SNP marker 485_1_1286 as called by SAMtools mpileup and bcftools with default parameters from M. roreri isolates C13. PCR-amplicons were cloned into pCR4-TOPO vector followed by transformation into DH5-alpha Escherichia coli and 9–11 clones from each amplicon were randomly selected to be sequenced from both ends using M13F and M13R primers. ClustalW2 ( Larkin et al., 2007 ) was used to compare the DNA sequences of each clone and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program ( http://phylogeny.lirmm.fr/ ) and the neighbor-joining method.

    Journal: Frontiers in Microbiology

    Article Title: Combination of RNAseq and SNP nanofluidic array reveals the center of genetic diversity of cacao pathogen Moniliophthora roreri in the upper Magdalena Valley of Colombia and its clonality

    doi: 10.3389/fmicb.2015.00850

    Figure Lengend Snippet: Sanger sequencing of partial genomic DNA flanking the putative heterozygous SNP marker 485_1_1286 as called by SAMtools mpileup and bcftools with default parameters from M. roreri isolates C13. PCR-amplicons were cloned into pCR4-TOPO vector followed by transformation into DH5-alpha Escherichia coli and 9–11 clones from each amplicon were randomly selected to be sequenced from both ends using M13F and M13R primers. ClustalW2 ( Larkin et al., 2007 ) was used to compare the DNA sequences of each clone and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program ( http://phylogeny.lirmm.fr/ ) and the neighbor-joining method.

    Article Snippet: The purified amplicons were cloned into pCR4-TOPO vector (TOPO TA Cloning Kit, Life Technologies, USA) and transformed in to DH5-alpha Escherichia Coli (New England Biolab, USA) according to the manufacturers protocol.

    Techniques: Sequencing, Marker, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Transformation Assay, Amplification, Generated