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Millipore ndei
Ndei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1089 article reviews
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ndei - by Bioz Stars, 2020-02
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Molecular Cloning:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: .. Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For this purpose, jerF_pMK-T was double digested by NdeI (New England Biolabs, Ipswich, MA, USA) and EcoRI (New England Biolabs).

Amplification:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For cloning into pET-20b(+) (Novagen), jerF was amplified by PCR under standard conditions using jerF_pMK-T as a template with primers as follows: forward, 5′-AGG CTC GAG TGC CGG ACT TTC GGT GC-3′; reverse, 5′-TGA GAT CTC ATA TGC GTA CCA GTG ATG C-3′.

Clone Assay:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: .. Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For this purpose, jerF_pMK-T was double digested by NdeI (New England Biolabs, Ipswich, MA, USA) and EcoRI (New England Biolabs).

Positron Emission Tomography:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: .. Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For this purpose, jerF_pMK-T was double digested by NdeI (New England Biolabs, Ipswich, MA, USA) and EcoRI (New England Biolabs).

Expressing:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. The gel-purified insert was ligated into NdeI/EcoRI-treated pET-28a(+) and pCOLD-I respectively using T4 DNA Ligase (Thermo Fisher Scientific, Waltham, MA, USA) to generate recombinant plasmids jerF_pET-28a(+) and jerF pCOLD-I for expression of N-terminal His6 -tagged fusion proteins.

Polymerase Chain Reaction:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For cloning into pET-20b(+) (Novagen), jerF was amplified by PCR under standard conditions using jerF_pMK-T as a template with primers as follows: forward, 5′-AGG CTC GAG TGC CGG ACT TTC GGT GC-3′; reverse, 5′-TGA GAT CTC ATA TGC GTA CCA GTG ATG C-3′.

Activated Clotting Time Assay:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For cloning into pET-20b(+) (Novagen), jerF was amplified by PCR under standard conditions using jerF_pMK-T as a template with primers as follows: forward, 5′-AGG CTC GAG TGC CGG ACT TTC GGT GC-3′; reverse, 5′-TGA GAT CTC ATA TGC GTA CCA GTG ATG C-3′.

Recombinant:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. The gel-purified insert was ligated into NdeI/EcoRI-treated pET-28a(+) and pCOLD-I respectively using T4 DNA Ligase (Thermo Fisher Scientific, Waltham, MA, USA) to generate recombinant plasmids jerF_pET-28a(+) and jerF pCOLD-I for expression of N-terminal His6 -tagged fusion proteins.

Plasmid Preparation:

Article Title: Characterisation of the Broadly-Specific O-Methyl-transferase JerF from the Late Stages of Jerangolid Biosynthesis
Article Snippet: .. Molecular Cloning A codon-optimised gene for jerF was obtained as a plasmid jerF_pMK-T (Invitrogen, Waltham, MA, USA) provided with the NdeI (5′-CAT ATG-3′) and EcoRI (5′-GAA TTC-3′) restriction sites used for standard restriction cloning into pET-28a(+) (Novagen, Billerica, MA, USA) and pCOLD-I (Takara Bio USA Inc., Mountain View, CA, USA). .. For this purpose, jerF_pMK-T was double digested by NdeI (New England Biolabs, Ipswich, MA, USA) and EcoRI (New England Biolabs).

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  • 95
    Millipore nde i
    Determination of the crossover site. (A) Sequence of the minimal attP and attB sites, with common nucleotides marked with dots. The central three nucleotides are numbered 1 to 3 in this figure. (B) In vitro intramolecular deletion reactions (30 minutes) on supercoiled plasmids with attB sites containing mutations at the central three nucleotides as designated. P×B, wild-type control. The products were digested with Xho I and Bam H1 to reveal deletion products (Figure 1D ). (C) Results of in vivo deletion reactions between attP and mutated attB sites. White colonies result from site-specific deletions and blue colonies signify absence of deletions. (D) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB at the designated positions. Parental and deletion product bands are denoted. (E) Results of in vivo recombination reactions between mutated attP and attB sites. Because G1C and G2C mutations create symmetrical cores, inversions between att sites oriented in an antiparallel configuration (G) form along with deletions. Inversion products retain the Lac + (blue) phenotype. (F) Diagram of synapsis between attP (G1C) and attB (G1C) in the standard parallel orientation generating attL and attR upon <t>DNA</t> exchange. Productive recombination between wild-type attP and attB sites containing the asymmetric GG core nucleotides only occur by this pathway. (G) Diagram of an antiparallel synapsis between attP (G1C) and attB (G1C) generating the hybrid attL * and attR * sites upon DNA exchange. (H) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB within the core nucleotides. The reaction products were digested with Bam HI, Nde I, and Sca I to reveal both deletions (doublet bands) and inversions as denoted.
    Nde I, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nde i/product/Millipore
    Average 95 stars, based on 903 article reviews
    Price from $9.99 to $1999.99
    nde i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Millipore ndei site
    Determination of the crossover site. (A) Sequence of the minimal attP and attB sites, with common nucleotides marked with dots. The central three nucleotides are numbered 1 to 3 in this figure. (B) In vitro intramolecular deletion reactions (30 minutes) on supercoiled plasmids with attB sites containing mutations at the central three nucleotides as designated. P×B, wild-type control. The products were digested with Xho I and Bam H1 to reveal deletion products (Figure 1D ). (C) Results of in vivo deletion reactions between attP and mutated attB sites. White colonies result from site-specific deletions and blue colonies signify absence of deletions. (D) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB at the designated positions. Parental and deletion product bands are denoted. (E) Results of in vivo recombination reactions between mutated attP and attB sites. Because G1C and G2C mutations create symmetrical cores, inversions between att sites oriented in an antiparallel configuration (G) form along with deletions. Inversion products retain the Lac + (blue) phenotype. (F) Diagram of synapsis between attP (G1C) and attB (G1C) in the standard parallel orientation generating attL and attR upon <t>DNA</t> exchange. Productive recombination between wild-type attP and attB sites containing the asymmetric GG core nucleotides only occur by this pathway. (G) Diagram of an antiparallel synapsis between attP (G1C) and attB (G1C) generating the hybrid attL * and attR * sites upon DNA exchange. (H) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB within the core nucleotides. The reaction products were digested with Bam HI, Nde I, and Sca I to reveal both deletions (doublet bands) and inversions as denoted.
    Ndei Site, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei site/product/Millipore
    Average 90 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    ndei site - by Bioz Stars, 2020-02
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    80
    Millipore ndei noti sites
    Determination of the crossover site. (A) Sequence of the minimal attP and attB sites, with common nucleotides marked with dots. The central three nucleotides are numbered 1 to 3 in this figure. (B) In vitro intramolecular deletion reactions (30 minutes) on supercoiled plasmids with attB sites containing mutations at the central three nucleotides as designated. P×B, wild-type control. The products were digested with Xho I and Bam H1 to reveal deletion products (Figure 1D ). (C) Results of in vivo deletion reactions between attP and mutated attB sites. White colonies result from site-specific deletions and blue colonies signify absence of deletions. (D) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB at the designated positions. Parental and deletion product bands are denoted. (E) Results of in vivo recombination reactions between mutated attP and attB sites. Because G1C and G2C mutations create symmetrical cores, inversions between att sites oriented in an antiparallel configuration (G) form along with deletions. Inversion products retain the Lac + (blue) phenotype. (F) Diagram of synapsis between attP (G1C) and attB (G1C) in the standard parallel orientation generating attL and attR upon <t>DNA</t> exchange. Productive recombination between wild-type attP and attB sites containing the asymmetric GG core nucleotides only occur by this pathway. (G) Diagram of an antiparallel synapsis between attP (G1C) and attB (G1C) generating the hybrid attL * and attR * sites upon DNA exchange. (H) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB within the core nucleotides. The reaction products were digested with Bam HI, Nde I, and Sca I to reveal both deletions (doublet bands) and inversions as denoted.
    Ndei Noti Sites, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei noti sites/product/Millipore
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ndei noti sites - by Bioz Stars, 2020-02
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    Image Search Results


    Determination of the crossover site. (A) Sequence of the minimal attP and attB sites, with common nucleotides marked with dots. The central three nucleotides are numbered 1 to 3 in this figure. (B) In vitro intramolecular deletion reactions (30 minutes) on supercoiled plasmids with attB sites containing mutations at the central three nucleotides as designated. P×B, wild-type control. The products were digested with Xho I and Bam H1 to reveal deletion products (Figure 1D ). (C) Results of in vivo deletion reactions between attP and mutated attB sites. White colonies result from site-specific deletions and blue colonies signify absence of deletions. (D) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB at the designated positions. Parental and deletion product bands are denoted. (E) Results of in vivo recombination reactions between mutated attP and attB sites. Because G1C and G2C mutations create symmetrical cores, inversions between att sites oriented in an antiparallel configuration (G) form along with deletions. Inversion products retain the Lac + (blue) phenotype. (F) Diagram of synapsis between attP (G1C) and attB (G1C) in the standard parallel orientation generating attL and attR upon DNA exchange. Productive recombination between wild-type attP and attB sites containing the asymmetric GG core nucleotides only occur by this pathway. (G) Diagram of an antiparallel synapsis between attP (G1C) and attB (G1C) generating the hybrid attL * and attR * sites upon DNA exchange. (H) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB within the core nucleotides. The reaction products were digested with Bam HI, Nde I, and Sca I to reveal both deletions (doublet bands) and inversions as denoted.

    Journal: Mobile DNA

    Article Title: The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase

    doi: 10.1186/1759-8753-4-2

    Figure Lengend Snippet: Determination of the crossover site. (A) Sequence of the minimal attP and attB sites, with common nucleotides marked with dots. The central three nucleotides are numbered 1 to 3 in this figure. (B) In vitro intramolecular deletion reactions (30 minutes) on supercoiled plasmids with attB sites containing mutations at the central three nucleotides as designated. P×B, wild-type control. The products were digested with Xho I and Bam H1 to reveal deletion products (Figure 1D ). (C) Results of in vivo deletion reactions between attP and mutated attB sites. White colonies result from site-specific deletions and blue colonies signify absence of deletions. (D) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB at the designated positions. Parental and deletion product bands are denoted. (E) Results of in vivo recombination reactions between mutated attP and attB sites. Because G1C and G2C mutations create symmetrical cores, inversions between att sites oriented in an antiparallel configuration (G) form along with deletions. Inversion products retain the Lac + (blue) phenotype. (F) Diagram of synapsis between attP (G1C) and attB (G1C) in the standard parallel orientation generating attL and attR upon DNA exchange. Productive recombination between wild-type attP and attB sites containing the asymmetric GG core nucleotides only occur by this pathway. (G) Diagram of an antiparallel synapsis between attP (G1C) and attB (G1C) generating the hybrid attL * and attR * sites upon DNA exchange. (H) In vitro intramolecular recombination reactions (30 minutes) with plasmid substrates containing identical changes within attP and attB within the core nucleotides. The reaction products were digested with Bam HI, Nde I, and Sca I to reveal both deletions (doublet bands) and inversions as denoted.

    Article Snippet: The A118 integrase coding sequence was amplified by PCR from the phage DNA and cloned between Nde I and Bam HI sites of pET11a and pET15b (EMD Millipore Billerica, MA USA) to give pRJ2186 and pRJ2184, respectively. pRJ2823 contains integrase residues 158 to 452 (C-terminus) in pET15b.

    Techniques: Sequencing, In Vitro, In Vivo, Plasmid Preparation