nd 1000 spectrophotometer  (Thermo Fisher)


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    Name:
    Fiberlite F10-4 x 1000 LEX Rotor
    Description:
    Protect samples, equipment and staff with the Thermo Scientific Fiberlite F10-4x1000 LEX Rotor, which features advanced sealing properties and lifting handles. This next generation high capacity Fiberlite rotor takes the lightweight design of carbon fiber to a new level, providing exceptional RCF performance for enhanced productivity. Superior ergonomics simplify everyday ease of handling while improving overall safety. 
    Catalog Number:
    096-041053
    Price:
    None
    Applications:
    Centrifuges|Lab Equipment
    Category:
    Instruments and Equipment, Centrifuges & Accessories, Centrifuge Rotors & Accessories
    Buy from Supplier
    Name:
    TX-1000 4 x Swinging Bucket Rotor
    Description:
    Process 4L in a traditional 3L general purpose centrifuge footprint with the Thermo Scientific TX-1000 rotor. High throughput capacities for processing more tubes in less runs saves time while reducing energy and wear. The rotor features Thermo Scientific Slide Coat finish, an advanced lubricant and corrosion resistant coating. Dual-purpose large bucket design accepts adapters for tubes or microplates.Save time — no need to swap buckets; save space —  no additional buckets to store; save money —  no additional buckets to purchase.
    Catalog Number:
    75003001
    Price:
    None
    Applications:
    Centrifuges|Lab Equipment
    Size:
    Each
    Category:
    Instruments and Equipment, Centrifuges & Accessories, Centrifuge Rotors & Accessories
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    Structured Review

    Thermo Fisher nd 1000 spectrophotometer
    (A) Microfluidic analyses of total RNA prepared from different life stages of Nylanderia pubens by the Trizol method. RNA standards and their corresponding sizes are provided in the left-most column. (B) PCR of a portion of the N. pubens ubiquin gene. DNase-treated RNA (from panel A) of larvae (lane 1 ) and adults (lane 2) was reverse transcribed and subsequently amplified by PCR. MW = molecular weight markers. Sizes shown on the left. (C) Results of spectrophotometric analysis of the RNA preparations from workers and larvae from the upper panel on an <t>ND-1000</t> spectrophotometer. Standard deviation values are indicated by error bars. The mean 260:280 nm ratio is provided for each life stage within the bar. High quality figures are available online.
    Process 4L in a traditional 3L general purpose centrifuge footprint with the Thermo Scientific TX-1000 rotor. High throughput capacities for processing more tubes in less runs saves time while reducing energy and wear. The rotor features Thermo Scientific Slide Coat finish, an advanced lubricant and corrosion resistant coating. Dual-purpose large bucket design accepts adapters for tubes or microplates.Save time — no need to swap buckets; save space —  no additional buckets to store; save money —  no additional buckets to purchase.
    https://www.bioz.com/result/nd 1000 spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 3704 article reviews
    Price from $9.99 to $1999.99
    nd 1000 spectrophotometer - by Bioz Stars, 2019-09
    99/100 stars

    Images

    1) Product Images from "Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens"

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens

    Journal: Journal of Insect Science

    doi: 10.1673/031.012.14601

    (A) Microfluidic analyses of total RNA prepared from different life stages of Nylanderia pubens by the Trizol method. RNA standards and their corresponding sizes are provided in the left-most column. (B) PCR of a portion of the N. pubens ubiquin gene. DNase-treated RNA (from panel A) of larvae (lane 1 ) and adults (lane 2) was reverse transcribed and subsequently amplified by PCR. MW = molecular weight markers. Sizes shown on the left. (C) Results of spectrophotometric analysis of the RNA preparations from workers and larvae from the upper panel on an ND-1000 spectrophotometer. Standard deviation values are indicated by error bars. The mean 260:280 nm ratio is provided for each life stage within the bar. High quality figures are available online.
    Figure Legend Snippet: (A) Microfluidic analyses of total RNA prepared from different life stages of Nylanderia pubens by the Trizol method. RNA standards and their corresponding sizes are provided in the left-most column. (B) PCR of a portion of the N. pubens ubiquin gene. DNase-treated RNA (from panel A) of larvae (lane 1 ) and adults (lane 2) was reverse transcribed and subsequently amplified by PCR. MW = molecular weight markers. Sizes shown on the left. (C) Results of spectrophotometric analysis of the RNA preparations from workers and larvae from the upper panel on an ND-1000 spectrophotometer. Standard deviation values are indicated by error bars. The mean 260:280 nm ratio is provided for each life stage within the bar. High quality figures are available online.

    Techniques Used: Polymerase Chain Reaction, Amplification, Molecular Weight, Spectrophotometry, Standard Deviation

    2) Product Images from "Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling"

    Article Title: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-5-9

    Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) NanoDrop ® ND-1000 Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.
    Figure Legend Snippet: Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) NanoDrop ® ND-1000 Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.

    Techniques Used: Purification, Spectrophotometry

    3) Product Images from "A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue"

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152157

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Figure Legend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Techniques Used: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    4) Product Images from "A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue"

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152157

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Figure Legend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Techniques Used: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    5) Product Images from "A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches"

    Article Title: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches

    Journal: Virology Journal

    doi: 10.1186/1743-422X-10-201

    Analysis of DDZ-AuNP sensitivity. A) DDZ-M-AuNP colorimetric assays were performed on DENV-2 NGC titers of 1 × 10 1 , 1 × 10 2 , 1 × 10 4 , and 1 × 10 6 to determine the limits of detection. Samples were assembled as described in Methods, incubated at 37°C for 30 minutes and photographed. Results show that the DDZ-M-AuNP colorimetric assay is capable of detecting DENV-2 at a titer as low as 1 × 10 1 . DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DDZ-AuNP sensitivity by UV/Vis spectrophotometry. Ten microliters (10 uL) of cell culture fluid from Mock, DENV-2 NGC (titers of 1 × 10 1 , 1 × 10 2 1 × 10 6 /mL), or CHIKV vaccine strain 181/25 (1 × 10 6/ mL) infected C6/36 cells, or DENV serially diluted from 1 × 10 1 (Dil1 through Dil5) or were added to separate mixtures as described in A) . UV/Vis spectrophotometric analysis was performed using the ND-1000 spectrophotometer at an absorbance of 520 nm. Absorbance measurements were graphed in log scale to illustrate sensitivity and accuracy of the colorimetric DENV detection method.
    Figure Legend Snippet: Analysis of DDZ-AuNP sensitivity. A) DDZ-M-AuNP colorimetric assays were performed on DENV-2 NGC titers of 1 × 10 1 , 1 × 10 2 , 1 × 10 4 , and 1 × 10 6 to determine the limits of detection. Samples were assembled as described in Methods, incubated at 37°C for 30 minutes and photographed. Results show that the DDZ-M-AuNP colorimetric assay is capable of detecting DENV-2 at a titer as low as 1 × 10 1 . DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DDZ-AuNP sensitivity by UV/Vis spectrophotometry. Ten microliters (10 uL) of cell culture fluid from Mock, DENV-2 NGC (titers of 1 × 10 1 , 1 × 10 2 1 × 10 6 /mL), or CHIKV vaccine strain 181/25 (1 × 10 6/ mL) infected C6/36 cells, or DENV serially diluted from 1 × 10 1 (Dil1 through Dil5) or were added to separate mixtures as described in A) . UV/Vis spectrophotometric analysis was performed using the ND-1000 spectrophotometer at an absorbance of 520 nm. Absorbance measurements were graphed in log scale to illustrate sensitivity and accuracy of the colorimetric DENV detection method.

    Techniques Used: Incubation, Colorimetric Assay, Spectrophotometry, Cell Culture, Infection

    Assessment of DDZ-AuNP specificity. A) DENV-2 and CHIKV (1 × 106/mL each) were placed in a buffered solution containing 10 mM MgCl2, 1 × 105 DDZ-AuNP particles/mL, 1.5 M NaCl, and 0.5% (w/v) SDS. Eppendorf tubes containing these mixes were incubated at 37°C for 30 minutes and photographed. CHIKV = chikungunya virus. DENV-2 = dengue virus serotype 2, DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DENV detection by UV/Vis Spectrophotometry. Samples were assembled as was performed for in A, mixed, incubated at 37°C for 5 minutes, and spectrophotometric analysis was performed using the ND-1000 spectrophotometer. C) Detection of DENV by DDZ-M-AuNP in comparison to several other flaviviruses. The specificity of our DDZ-M-AuNP device to detect DENV and not other fellow flavivirus members YFV, JEV or ZV was determined as described earlier (see Figure 4 ). D) An alignment was performed on consensus sequences of each of the four DENV serotypes to determine the most optimal regions for the design of serotype specific DDZ-AuNP devices by determining the region of least conservation one serotype has with the other DENV serotypes. E) Colorimetric serotype-specific detection of DENV. Cell culture supernatants from C6/36 cells mock infected (Mock), or from cells infected with either DENV serotypes 1 through 4 or CHIKV (1 × 106/mL each) were placed in buffered solutions containing the necessary cofactors and AuNPs tethered with our all purpose DENV serotype specific DNAzyme, DDZ-M, or one of the serotype-specific DDZs (designated DDZ-1 through-4) designed to specifically target the corresponding DENV serotype (Table 1 ). Eppendorf tubes containing these mixes were incubated at 37°C for 5 minutes and photographed.
    Figure Legend Snippet: Assessment of DDZ-AuNP specificity. A) DENV-2 and CHIKV (1 × 106/mL each) were placed in a buffered solution containing 10 mM MgCl2, 1 × 105 DDZ-AuNP particles/mL, 1.5 M NaCl, and 0.5% (w/v) SDS. Eppendorf tubes containing these mixes were incubated at 37°C for 30 minutes and photographed. CHIKV = chikungunya virus. DENV-2 = dengue virus serotype 2, DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DENV detection by UV/Vis Spectrophotometry. Samples were assembled as was performed for in A, mixed, incubated at 37°C for 5 minutes, and spectrophotometric analysis was performed using the ND-1000 spectrophotometer. C) Detection of DENV by DDZ-M-AuNP in comparison to several other flaviviruses. The specificity of our DDZ-M-AuNP device to detect DENV and not other fellow flavivirus members YFV, JEV or ZV was determined as described earlier (see Figure 4 ). D) An alignment was performed on consensus sequences of each of the four DENV serotypes to determine the most optimal regions for the design of serotype specific DDZ-AuNP devices by determining the region of least conservation one serotype has with the other DENV serotypes. E) Colorimetric serotype-specific detection of DENV. Cell culture supernatants from C6/36 cells mock infected (Mock), or from cells infected with either DENV serotypes 1 through 4 or CHIKV (1 × 106/mL each) were placed in buffered solutions containing the necessary cofactors and AuNPs tethered with our all purpose DENV serotype specific DNAzyme, DDZ-M, or one of the serotype-specific DDZs (designated DDZ-1 through-4) designed to specifically target the corresponding DENV serotype (Table 1 ). Eppendorf tubes containing these mixes were incubated at 37°C for 5 minutes and photographed.

    Techniques Used: Incubation, Spectrophotometry, Cell Culture, Infection

    Related Articles

    Amplification:

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens
    Article Snippet: For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ). .. For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: The cDNA was amplified for 17 cycles and purified using PureLink Quick PCR Purification Kit (Invitrogen, K310001). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. 8.5 ug of the cDNA from each sample were fragmented and labeling with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753) following the instructions of manufacturer.

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). .. Purified mRNAs were normalized by total mRNA concentration, and Qiagen one-step RT-PCR was conducted with primers for HO1, NQO1, GCLC, and GAPDH, which were purchased from Origene.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: When the SYBR Green signal reached a plateau after 27 cycles, the reaction was stopped. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. 8 µg of cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 250K Nsp Assay Kit (Affymetrix, 900753).

    Synthesized:

    Article Title: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
    Article Snippet: The oligoribonucleotide target was synthesized and HPLC-purified by Life Science Technologies. .. Quantification of these oligonucleotides was performed with the ND-1000 spectrophotometer from NanoDrop (Wilmington, DE).

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: Total RNA was isolated from all tissues under RNAse-free conditions (RNAse OUT™, GBiosciences, St. Louis, MO, USA) following recommended TRI-Reagent® (Ambion, Grand Island, NY, USA) protocols for high lipid samples. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. Lep, LEPR, LEPR-b, PNPLA2 , and LIPE , were amplified from cetacean, rodent and human cDNAs using specifically designed gene and species-specific primers ( Online Resource 1 ).

    Article Title: Endophilin A2: A Potential Link to Adiposity and Beyond
    Article Snippet: RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK). .. RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK).

    Quantitative RT-PCR:

    Article Title: Endophilin A2: A Potential Link to Adiposity and Beyond
    Article Snippet: RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK). .. RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK).

    SYBR Green Assay:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: When the SYBR Green signal reached a plateau after 27 cycles, the reaction was stopped. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Article Title: Efficient derivation of microglia-like cells from human pluripotent stem cells
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophotometer. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) with random hexamer primers.

    Article Title: Annatto-extracted tocotrienols improve glucose homeostasis and bone properties in high-fat diet-induced type 2 diabetic mice by decreasing the inflammatory response
    Article Snippet: RNA was quantified using the Nanodrop ND 1000 Spectrophotometer. .. RNA was quantified using the Nanodrop ND 1000 Spectrophotometer.

    Microarray:

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

    Incubation:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. 8.5 ug of the cDNA from each sample were fragmented and labeling with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753) following the instructions of manufacturer.

    Expressing:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Paragraph title: Gene Expression Profiling and data analysis ... Samples were quantified using the NanoDrop ND-1000 spectrophotometer.

    Article Title: A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
    Article Snippet: Paragraph title: Expression profiling and differential expression analysis ... RNA quality and quantity were assessed by Nanodrop ND-1000 spectrophotometer and Agilent bioanalyzer, respectively.

    Article Title: Annatto-extracted tocotrienols improve glucose homeostasis and bone properties in high-fat diet-induced type 2 diabetic mice by decreasing the inflammatory response
    Article Snippet: Paragraph title: Measurement of mRNA expression in liver ... RNA was quantified using the Nanodrop ND 1000 Spectrophotometer.

    Article Title: Endophilin A2: A Potential Link to Adiposity and Beyond
    Article Snippet: Paragraph title: Human EA2 gene expression in paired subcutaneous and visceral adipose tissue ... RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK).

    Derivative Assay:

    Article Title: Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry
    Article Snippet: A secondary Chilean replication population was derived from GBC patients, with more than 90% presenting gallstones. .. DNA concentration and integrity were determined by the NanoDrop ND-1000® Spectrophotometer and by agarose gel electrophoresis, respectively.

    Hybridization:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

    High Performance Liquid Chromatography:

    Article Title: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
    Article Snippet: The oligoribonucleotide target was synthesized and HPLC-purified by Life Science Technologies. .. Quantification of these oligonucleotides was performed with the ND-1000 spectrophotometer from NanoDrop (Wilmington, DE).

    Gas Chromatography:

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Samples were quantified using the NanoDrop ND-1000 spectrophotometer.

    Immunoprecipitation:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Library preparation and amplification were performed following the distributor’s (Sigma-Aldrich) recommendations for WTA2 from purified immunoprecipitated or diluted input RNA. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Generated:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. The arrays were scanned in the GeneAtlas Imaging Station.

    Imaging:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. After hybridization for 16h at 45ºC, washing and staining was performed in the Affymetrix GeneAtlas Fluidics Station.

    Protein Concentration:

    Article Title: Structure of human cytoplasmic dynein-2 primed for its powerstroke
    Article Snippet: The supernatant was mixed with 1M K2 HPO4 , 3M KOH and 100% acetic acid (final concentrations were 125 mM, 375 mM, and 0.5 M respectively). .. Total nucleotide content and protein concentration before extraction were measured using NanoDrop ND-1000 spectrophotometer. .. Dynein motor domain constructs were purified as described above and diluted into EM assay buffer (50 mM Tris HCl pH 8, 150 mM KOAc, 2 mM MgOAc, 1 mM EGTA and 0.1 mM DTT) to a final concentration of 30 nM.

    Polymerase Chain Reaction:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: Total RNA was isolated from all tissues under RNAse-free conditions (RNAse OUT™, GBiosciences, St. Louis, MO, USA) following recommended TRI-Reagent® (Ambion, Grand Island, NY, USA) protocols for high lipid samples. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. Lep, LEPR, LEPR-b, PNPLA2 , and LIPE , were amplified from cetacean, rodent and human cDNAs using specifically designed gene and species-specific primers ( Online Resource 1 ).

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens
    Article Snippet: For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ). .. For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: The cDNA was amplified for 17 cycles and purified using PureLink Quick PCR Purification Kit (Invitrogen, K310001). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: Paragraph title: Reverse transcription PCR (RT-PCR) analysis of HepG2 cells treated with Nrf2 delivered by DARTs ... RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA).

    Article Title: Efficient derivation of microglia-like cells from human pluripotent stem cells
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophotometer. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) with random hexamer primers.

    Nucleic Acid Electrophoresis:

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). .. Purified mRNAs were normalized by total mRNA concentration, and Qiagen one-step RT-PCR was conducted with primers for HO1, NQO1, GCLC, and GAPDH, which were purchased from Origene.

    Isolation:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: Paragraph title: RNA isolation/cDNA synthesis ... RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Paragraph title: RNA isolation, Whole Transcriptome Amplification (WTA) and microarrays processing ... Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Total RNA was isolated from HEK cells as described above. .. Samples were quantified using the NanoDrop ND-1000 spectrophotometer.

    Article Title: Expression of selected genes isolated from whole blood, liver and obex in lambs with experimental classical scrapie and healthy controls, showing a systemic innate immune response at the clinical end-stage
    Article Snippet: Paragraph title: Blood and tissue collection and RNA isolation ... Total RNA was quantified by UV spectrometry at 260 nm using a NanoDrop ND-1000 spectrophotometer (Saveen and Werner AB, Limhamn, Sweden).

    Purification:

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens
    Article Snippet: Microfluidic assays were completed immediately after RNA extraction using a 1 µl volume of purified sample. .. For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: The cDNA was amplified for 17 cycles and purified using PureLink Quick PCR Purification Kit (Invitrogen, K310001). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: When the SYBR Green signal reached a plateau after 27 cycles, the reaction was stopped. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. 8 µg of cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 250K Nsp Assay Kit (Affymetrix, 900753).

    Article Title: Structure of human cytoplasmic dynein-2 primed for its powerstroke
    Article Snippet: Nucleotides were extracted from dynein-2 samples that had been purified as described above. .. Total nucleotide content and protein concentration before extraction were measured using NanoDrop ND-1000 spectrophotometer.

    Article Title: Expression of selected genes isolated from whole blood, liver and obex in lambs with experimental classical scrapie and healthy controls, showing a systemic innate immune response at the clinical end-stage
    Article Snippet: Total RNA was extracted and purified from stabilized blood samples manually, using PAXgene® Blood miRNA Kit (Qiagen/BD Company) according to manufacturer’s instructions using the protocol for manual purification. .. Total RNA was quantified by UV spectrometry at 260 nm using a NanoDrop ND-1000 spectrophotometer (Saveen and Werner AB, Limhamn, Sweden).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens
    Article Snippet: Paragraph title: RNA analyses and RT-PCR ... For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ).

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: Paragraph title: Reverse transcription PCR (RT-PCR) analysis of HepG2 cells treated with Nrf2 delivered by DARTs ... RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA).

    cDNA Library Assay:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: cDNA library preparation and amplification were performed according to the manufacturer’s instructions (Sigma-Aldrich) for the WTA2 kit from 25 ng starting material. .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    Mouse Assay:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Total tissue RNA was extracted from prefrontal cortex - BA8/9 of CTRL (n = 15) and idiopathic ASD patients (n = 16) and striatum, cortex or forebrain from Control, CPEB4 KOGT /+, TgCPEB4∆4, CPEB4 KO/+ and TgCPEB4∆4:CPEB4 KOGT /+, mice using the Maxwell® 16 LEV simplyRNA Tissue Kit (Promega, AS1280). .. Quantification and quality of RNA was done on a Nanodrop ND-1000 spectrophotometer and Nanodrop 1000 v.3.7.1 (Thermo Scientific).

    Software:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. The arrays were scanned in the GeneAtlas Imaging Station.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Samples were quantified using the NanoDrop ND-1000 spectrophotometer.

    Real-time Polymerase Chain Reaction:

    Article Title: Efficient derivation of microglia-like cells from human pluripotent stem cells
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and quantitative PCR ... Total RNA concentrations were measured using NanoDrop ND-1000 spectrophotometer.

    Article Title: Annatto-extracted tocotrienols improve glucose homeostasis and bone properties in high-fat diet-induced type 2 diabetic mice by decreasing the inflammatory response
    Article Snippet: RNA was quantified using the Nanodrop ND 1000 Spectrophotometer. .. RNA was quantified using the Nanodrop ND 1000 Spectrophotometer.

    Article Title: Endophilin A2: A Potential Link to Adiposity and Beyond
    Article Snippet: RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK). .. RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK).

    RNA Extraction:

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens
    Article Snippet: Microfluidic assays were completed immediately after RNA extraction using a 1 µl volume of purified sample. .. For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ).

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: Paragraph title: 3.2 Analysis of RNA following RNA Extraction ... The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... Quantification and quality of RNA was done on a Nanodrop ND-1000 spectrophotometer and Nanodrop 1000 v.3.7.1 (Thermo Scientific).

    Article Title: Efficient derivation of microglia-like cells from human pluripotent stem cells
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and quantitative PCR ... Total RNA concentrations were measured using NanoDrop ND-1000 spectrophotometer.

    Agarose Gel Electrophoresis:

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). .. Purified mRNAs were normalized by total mRNA concentration, and Qiagen one-step RT-PCR was conducted with primers for HO1, NQO1, GCLC, and GAPDH, which were purchased from Origene.

    Article Title: Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry
    Article Snippet: After delimiting the healthy tissue, either adjacent to the tumor or other non-tumoral tissues such as liver or cystic ganglion, blocks were selected for sequential cuts of 10 µm thickness and used for extraction of genomic DNA. .. DNA concentration and integrity were determined by the NanoDrop ND-1000® Spectrophotometer and by agarose gel electrophoresis, respectively. .. We analyzed two European GSD population from Germany.

    Random Hexamer Labeling:

    Article Title: Efficient derivation of microglia-like cells from human pluripotent stem cells
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophotometer. .. For RNAseq, RNA was directly analyzed and quality checked before sequencing.

    Spectrophotometry:

    Article Title: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
    Article Snippet: The oligoribonucleotide target was synthesized and HPLC-purified by Life Science Technologies. .. Quantification of these oligonucleotides was performed with the ND-1000 spectrophotometer from NanoDrop (Wilmington, DE). .. Gold colloidal solutions containing 1.6 × 1012 particles/mL gold nanoparticles (AuNPs) with a diameter of 15 nm were purchased from Cytodiagnostics (Burlington, ON, CA).

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: Total RNA was isolated from all tissues under RNAse-free conditions (RNAse OUT™, GBiosciences, St. Louis, MO, USA) following recommended TRI-Reagent® (Ambion, Grand Island, NY, USA) protocols for high lipid samples. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. Lep, LEPR, LEPR-b, PNPLA2 , and LIPE , were amplified from cetacean, rodent and human cDNAs using specifically designed gene and species-specific primers ( Online Resource 1 ).

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: The supernatant was discarded and RNA resuspended in RNase free H2 O and stored at -80°C. .. Samples were assessed for RNA quantity using NanoDrop ND-1000 Spectrophotometer, with the purity determined by absorbance ratio measurement at 260nm/280nm (ideal ratio of 2.0). .. Sample quality was determined from the RNA Integrity Number (RIN) on the Agilent 2100 Bioanalyser using either the Agilent RNA 6000 Nano Kit (samples greater than 25ng/μl) or Agilent RNA 6000 Pico Kit (samples less than 25ng/μl).

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens
    Article Snippet: RNA size standards (200 to 6,000 nucleotides) and a positive RNA control (larval N. pubens RNA of known integrity) were included for each experiment. .. For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ). .. The 260:280 nm ratio and quantity of RNA were determined.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: The cDNA was amplified for 17 cycles and purified using PureLink Quick PCR Purification Kit (Invitrogen, K310001). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. 8.5 ug of the cDNA from each sample were fragmented and labeling with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753) following the instructions of manufacturer.

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: The quality and quantity of the extracted RNA and the ability to amplify taste genes was assessed. .. The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ). .. The fungiform papillae biopsy, tongue saliva, cheek saliva and ORAgene RNA techniques enabled a high RNA yield and high purity ( ).

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: HepG2 cells were treated with either free Nrf2 or DART-Nrf2 in culture media for 24 h and the RNA was extracted from the cells using the RNeasy® Mini Kit with additional DNase I digestion (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. .. RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). .. Purified mRNAs were normalized by total mRNA concentration, and Qiagen one-step RT-PCR was conducted with primers for HO1, NQO1, GCLC, and GAPDH, which were purchased from Origene.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Total tissue RNA was extracted from prefrontal cortex - BA8/9 of CTRL (n = 15) and idiopathic ASD patients (n = 16) and striatum, cortex or forebrain from Control, CPEB4 KOGT /+, TgCPEB4∆4, CPEB4 KO/+ and TgCPEB4∆4:CPEB4 KOGT /+, mice using the Maxwell® 16 LEV simplyRNA Tissue Kit (Promega, AS1280). .. Quantification and quality of RNA was done on a Nanodrop ND-1000 spectrophotometer and Nanodrop 1000 v.3.7.1 (Thermo Scientific). .. Retrotranscription (RT) reactions were performed using the iScript cDNA Synthesis kit (Bio-Rad, PN170-8891) following manufacturer´s instructions.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: When the SYBR Green signal reached a plateau after 27 cycles, the reaction was stopped. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. 8 µg of cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 250K Nsp Assay Kit (Affymetrix, 900753).

    Article Title: Efficient derivation of microglia-like cells from human pluripotent stem cells
    Article Snippet: Cells and tissues were homogenized and total RNA extracted using the RNeasy Micro kit (Qiagen) following the manufacturer’s instructions. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophotometer. .. For RNAseq, RNA was directly analyzed and quality checked before sequencing.

    Article Title: Structure of human cytoplasmic dynein-2 primed for its powerstroke
    Article Snippet: The supernatant was mixed with 1M K2 HPO4 , 3M KOH and 100% acetic acid (final concentrations were 125 mM, 375 mM, and 0.5 M respectively). .. Total nucleotide content and protein concentration before extraction were measured using NanoDrop ND-1000 spectrophotometer. .. Dynein motor domain constructs were purified as described above and diluted into EM assay buffer (50 mM Tris HCl pH 8, 150 mM KOAc, 2 mM MgOAc, 1 mM EGTA and 0.1 mM DTT) to a final concentration of 30 nM.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Total RNA was isolated from HEK cells as described above. .. Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

    Article Title: Expression of selected genes isolated from whole blood, liver and obex in lambs with experimental classical scrapie and healthy controls, showing a systemic innate immune response at the clinical end-stage
    Article Snippet: Total RNA was isolated from 20 to 25 mg tissue sections, lysed and homogenized, from the posterior obex region in the brain and from the liver using RNeasy® Lipid Tissue Midi Kit and (Qiagen) and RNeasy® Lipid Tissue Mini Kit (Qiagen), respectively, including digestion of DNA using RNase-Free DNase kit (Qiagen). .. Total RNA was quantified by UV spectrometry at 260 nm using a NanoDrop ND-1000 spectrophotometer (Saveen and Werner AB, Limhamn, Sweden). .. In addition, for each sample 260/280 and 260/230 ratios were provided, enabling RNA sample purity estimations.

    Article Title: A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
    Article Snippet: Following exposure, cells were lysed in RNAlater (Ambion), and RNA was isolated using the Qiagen miniprep RNA isolation kit with column DNAse treatment. .. RNA quality and quantity were assessed by Nanodrop ND-1000 spectrophotometer and Agilent bioanalyzer, respectively. .. Paired RNA samples, selected based on RNA quality and quantity, were amplified and biotin labeled using the Illumina TotalPrep-96 RNA amplification kit, hybridized to Illumina HumanRef-8v3 beadarrays (Illumina), and scanned using an Illumina BeadXpress reader.

    Article Title: Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry
    Article Snippet: After delimiting the healthy tissue, either adjacent to the tumor or other non-tumoral tissues such as liver or cystic ganglion, blocks were selected for sequential cuts of 10 µm thickness and used for extraction of genomic DNA. .. DNA concentration and integrity were determined by the NanoDrop ND-1000® Spectrophotometer and by agarose gel electrophoresis, respectively. .. We analyzed two European GSD population from Germany.

    Article Title: Annatto-extracted tocotrienols improve glucose homeostasis and bone properties in high-fat diet-induced type 2 diabetic mice by decreasing the inflammatory response
    Article Snippet: On-column DNA digestion was performed using QIAGEN’s RNase-free DNase set. .. RNA was quantified using the Nanodrop ND 1000 Spectrophotometer. .. 2 µg RNA was reverse transcribed to produce cDNA.

    Article Title: Endophilin A2: A Potential Link to Adiposity and Beyond
    Article Snippet: Extraction was followed by a DNase digestion step. .. RNA was quantitated using a Nanodrop ND-1000 spectrophotometer (LabTec, UK). .. RNA quality was further evaluated via agarose gel electrophoresis and visual inspection of the 28S and 18S ribosomal RNA bands.

    Article Title: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling
    Article Snippet: To each reaction 12.5 μl of 7.5 M LiCl was added (2.5 M final concentration). .. The NanoDrop® ND-1000 Spectrophotometer (NanoDrop Technologies) was used to determine: 260/280 ratio, to assess total RNA and aRNA purity; 320/550 and 320/650 ratios, to evaluate Cy3 and Cy5-labelled aRNAs respectively. .. Quality of total RNA and patterns of amplified or labelled aRNA were evaluated using the Agilent-2100 Bioanalyzer with the RNA 6000 Nano Lapchip® kit (Agilent Technologies) and also by 1% denaturing agarose/formamide gels.

    Sampling:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA.

    Produced:

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ). .. The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ).

    Concentration Assay:

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: HepG2 cells were treated with either free Nrf2 or DART-Nrf2 in culture media for 24 h and the RNA was extracted from the cells using the RNeasy® Mini Kit with additional DNase I digestion (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. .. RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). .. Purified mRNAs were normalized by total mRNA concentration, and Qiagen one-step RT-PCR was conducted with primers for HO1, NQO1, GCLC, and GAPDH, which were purchased from Origene.

    Article Title: Structure of human cytoplasmic dynein-2 primed for its powerstroke
    Article Snippet: Briefly, concentrated protein was precipitated by adding HClO4 to a final concentration of 0.5 M. The sample was vortexed and centrifuged for 10 min at 4 °C and 14000×g. .. Total nucleotide content and protein concentration before extraction were measured using NanoDrop ND-1000 spectrophotometer.

    Article Title: Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry
    Article Snippet: After delimiting the healthy tissue, either adjacent to the tumor or other non-tumoral tissues such as liver or cystic ganglion, blocks were selected for sequential cuts of 10 µm thickness and used for extraction of genomic DNA. .. DNA concentration and integrity were determined by the NanoDrop ND-1000® Spectrophotometer and by agarose gel electrophoresis, respectively. .. We analyzed two European GSD population from Germany.

    Standard Deviation:

    Article Title: Expression of selected genes isolated from whole blood, liver and obex in lambs with experimental classical scrapie and healthy controls, showing a systemic innate immune response at the clinical end-stage
    Article Snippet: Total RNA was quantified by UV spectrometry at 260 nm using a NanoDrop ND-1000 spectrophotometer (Saveen and Werner AB, Limhamn, Sweden). .. Total RNA was quantified by UV spectrometry at 260 nm using a NanoDrop ND-1000 spectrophotometer (Saveen and Werner AB, Limhamn, Sweden).

    Staining:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    Article Title: In vivo delivery of transcription factors with multifunctional oligonucleotides
    Article Snippet: RNA purity and concentration were determined by measuring the optical density using an ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA). .. Purified mRNAs were normalized by total mRNA concentration, and Qiagen one-step RT-PCR was conducted with primers for HO1, NQO1, GCLC, and GAPDH, which were purchased from Origene.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

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    Thermo Fisher nd 1000 spectrophotometer
    Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nanodrop nd 1000 spectrophotometer
    Nanodrop Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop nd 1000 spectrophotometer/product/Thermo Fisher
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    Thermo Fisher nd 1000 spectrophometer
    Nd 1000 Spectrophometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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