nd 1000  (Thermo Fisher)


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    Name:
    Fiberlite F10-4 x 1000 LEX Rotor
    Description:
    Protect samples, equipment and staff with the Thermo Scientific Fiberlite F10-4x1000 LEX Rotor, which features advanced sealing properties and lifting handles. This next generation high capacity Fiberlite rotor takes the lightweight design of carbon fiber to a new level, providing exceptional RCF performance for enhanced productivity. Superior ergonomics simplify everyday ease of handling while improving overall safety. 
    Catalog Number:
    096-041053
    Price:
    None
    Applications:
    Centrifuges|Lab Equipment
    Category:
    Instruments and Equipment, Centrifuges & Accessories, Centrifuge Rotors & Accessories
    Score:
    85
    Buy from Supplier
    Name:
    TX-1000 4 x Swinging Bucket Rotor
    Description:
    Process 4L in a traditional 3L general purpose centrifuge footprint with the Thermo Scientific TX-1000 rotor. High throughput capacities for processing more tubes in less runs saves time while reducing energy and wear. The rotor features Thermo Scientific Slide Coat finish, an advanced lubricant and corrosion resistant coating. Dual-purpose large bucket design accepts adapters for tubes or microplates.Save time — no need to swap buckets; save space —  no additional buckets to store; save money —  no additional buckets to purchase.
    Catalog Number:
    75003001
    Price:
    None
    Applications:
    Centrifuges|Lab Equipment
    Size:
    Each
    Category:
    Instruments and Equipment, Centrifuges & Accessories, Centrifuge Rotors & Accessories
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher nd 1000
    Extracted RNA yield and purity . Yield and absorption ratios for the different extraction methods were determined. For each method, 6 cyanobacteria aliquots were used for RNA extraction and 1 μl analysed using the NanoDrop <t>ND-1000</t> UV/Vis spectrophotometer. The resulting bars shown are the average, and the standard deviation, from values obtained for each extraction method.
    Process 4L in a traditional 3L general purpose centrifuge footprint with the Thermo Scientific TX-1000 rotor. High throughput capacities for processing more tubes in less runs saves time while reducing energy and wear. The rotor features Thermo Scientific Slide Coat finish, an advanced lubricant and corrosion resistant coating. Dual-purpose large bucket design accepts adapters for tubes or microplates.Save time — no need to swap buckets; save space —  no additional buckets to store; save money —  no additional buckets to purchase.
    https://www.bioz.com/result/nd 1000/product/Thermo Fisher
    Average 99 stars, based on 621 article reviews
    Price from $9.99 to $1999.99
    nd 1000 - by Bioz Stars, 2019-11
    99/100 stars

    Images

    1) Product Images from "Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria"

    Article Title: Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria

    Journal: BMC Molecular Biology

    doi: 10.1186/1471-2199-10-79

    Extracted RNA yield and purity . Yield and absorption ratios for the different extraction methods were determined. For each method, 6 cyanobacteria aliquots were used for RNA extraction and 1 μl analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting bars shown are the average, and the standard deviation, from values obtained for each extraction method.
    Figure Legend Snippet: Extracted RNA yield and purity . Yield and absorption ratios for the different extraction methods were determined. For each method, 6 cyanobacteria aliquots were used for RNA extraction and 1 μl analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting bars shown are the average, and the standard deviation, from values obtained for each extraction method.

    Techniques Used: RNA Extraction, Spectrophotometry, Standard Deviation

    Extraction contamination analysis . Absorption spectra in the UV-region for purified RNA using 6 different extraction methods. For each method, 6 cyanobacteria aliquots were used for RNA extraction. From all obtained RNA samples, 1 μl was analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting lines shown are averaged from values obtained for each extraction method.
    Figure Legend Snippet: Extraction contamination analysis . Absorption spectra in the UV-region for purified RNA using 6 different extraction methods. For each method, 6 cyanobacteria aliquots were used for RNA extraction. From all obtained RNA samples, 1 μl was analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting lines shown are averaged from values obtained for each extraction method.

    Techniques Used: Purification, RNA Extraction, Spectrophotometry

    2) Product Images from "Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria"

    Article Title: Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria

    Journal: BMC Molecular Biology

    doi: 10.1186/1471-2199-10-79

    Extracted RNA yield and purity . Yield and absorption ratios for the different extraction methods were determined. For each method, 6 cyanobacteria aliquots were used for RNA extraction and 1 μl analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting bars shown are the average, and the standard deviation, from values obtained for each extraction method.
    Figure Legend Snippet: Extracted RNA yield and purity . Yield and absorption ratios for the different extraction methods were determined. For each method, 6 cyanobacteria aliquots were used for RNA extraction and 1 μl analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting bars shown are the average, and the standard deviation, from values obtained for each extraction method.

    Techniques Used: RNA Extraction, Spectrophotometry, Standard Deviation

    Extraction contamination analysis . Absorption spectra in the UV-region for purified RNA using 6 different extraction methods. For each method, 6 cyanobacteria aliquots were used for RNA extraction. From all obtained RNA samples, 1 μl was analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting lines shown are averaged from values obtained for each extraction method.
    Figure Legend Snippet: Extraction contamination analysis . Absorption spectra in the UV-region for purified RNA using 6 different extraction methods. For each method, 6 cyanobacteria aliquots were used for RNA extraction. From all obtained RNA samples, 1 μl was analysed using the NanoDrop ND-1000 UV/Vis spectrophotometer. The resulting lines shown are averaged from values obtained for each extraction method.

    Techniques Used: Purification, RNA Extraction, Spectrophotometry

    Related Articles

    Amplification:

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: Each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3′ bias utilizing a mixture of oligo(dT) and random primers (Arraystar Flash RNA labeling kit, Arraystar). .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis. .. Briefly, mRNA was purified from total RNA after removal of rRNA.

    Whole Genome Amplification:

    Article Title: Molecular profiling of single circulating tumor cells with diagnostic intention
    Article Snippet: As a reference, we used four re-amplified high-quality WGA products of single diploid lymphocytes of a female healthy donor that were pooled. .. DNA yields and dye incorporation rates were quantified using the NanoDrop ND-1000.

    Expressing:

    Article Title: Autoimmune Gastro-Pancreatitis with Anti-Protein Disulfide Isomerase-Associated 2 Autoantibody in Aire-Deficient BALB/cAnN Mice
    Article Snippet: Paragraph title: Gene expression analyses in thymus and pancreas ... Total RNA from thymus and pancreas were prepared using ISOGEN (NIPPON GENE, Tokyo, Japan) and the concentration of total RNA was measured by NanoDrop ND-1000 (LMS, Tokyo, Japan).

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels. .. The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio  >  1·8 and  <  2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels.

    Article Title: Polytherapy with a combination of three repurposed drugs (PXT3003) down-regulates Pmp22 over-expression and improves myelination, axonal and functional parameters in models of CMT1A neuropathy
    Article Snippet: Paragraph title: Pmp22 mRNA expression in RT4 cells and in sciatic nerves of CMT1A rats (9 weeks of treatment, 17 weeks of age) ... RNA concentrations were estimated by NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA labeling and array hybridization were performed according to a one-color microarray-based gene expression analysis protocol (Agilent) with minor modifications. .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis. .. Arraystar Human LncRNA microarray v3.0 designed for global profiling of human LncRNAs (32,586) and protein-coding transcripts (26,109) covering all entries of Refseq, UCSC known genes and Gencode was employed.

    Picogreen Assay:

    Article Title: Epigenome-wide association studies identify DNA methylation associated with kidney function
    Article Snippet: Genomic DNA (200 ng) was purified using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Purified DNA quality and concentration were assessed with NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) and by Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA) prior to bisulfite conversion. .. DNA methylation was measured using the Infinium HumanMethylation450 Beadchip (Illumina, San Diego, CA), with 485,000 CpG sites across the genome examined.

    Quantitative RT-PCR:

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: Paragraph title: 2.2. RNA extraction, miRNA microarray analysis, and quantitative real-time PCR (qRT-PCR) ... The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels.

    Article Title: Utility of Translocator Protein (18 kDa) as a Molecular Imaging Biomarker to Monitor the Progression of Liver Fibrosis
    Article Snippet: Paragraph title: RNA preparation and qRT-PCR ... The quality of total RNA was checked by measuring the ratio of absorbance at 260/280 nm with a NanoDrop ND-1000 (LMS, Tokyo).

    Real-time Polymerase Chain Reaction:

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: Paragraph title: 2.2. RNA extraction, miRNA microarray analysis, and quantitative real-time PCR (qRT-PCR) ... The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels.

    Article Title: Polytherapy with a combination of three repurposed drugs (PXT3003) down-regulates Pmp22 over-expression and improves myelination, axonal and functional parameters in models of CMT1A neuropathy
    Article Snippet: RNA concentrations were estimated by NanoDrop ND-1000. .. RNA concentrations were estimated by NanoDrop ND-1000.

    Article Title: Type 2 diabetes and obesity induce similar transcriptional reprogramming in human myocytes
    Article Snippet: Paragraph title: Additional subjects, RNA isolation, and quantitative PCR ... Total RNA was dissolved in RNase-free water and quantified using a Nanodrop ND 1000 (Saveen biotech ApS, Arhus, Denmark).

    Microarray:

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: Paragraph title: 2.2. RNA extraction, miRNA microarray analysis, and quantitative real-time PCR (qRT-PCR) ... The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: Paragraph title: Human genome-wide microarray ... The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Article Title: Molecular profiling of single circulating tumor cells with diagnostic intention
    Article Snippet: DNA yields and dye incorporation rates were quantified using the NanoDrop ND-1000. .. Array CGH was performed on oligonucleotide-based SurePrint G3 Human CGH 4 × 180K microarray slides (design code: 022060) according the protocol provided by the manufacturer (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, version 7.1, December 2011) with slight modifications (Czyz et al , ).

    Article Title: Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development
    Article Snippet: Paragraph title: Reverse transcription and Agilent DNA microarray analysis ... The concentration of each sample is determined by NanoDrop ND-1000.

    Incubation:

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. .. A volume of 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide (Arraystar).

    Activity Assay:

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: The labeled cRNAs were purified by RNeasy Mini kit (Qiagen). .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. .. One microgram of each labeled cRNA was fragmented by adding 5 μl 10 × blocking agent and 1 μl of 25 × fragmentation buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE hybridization buffer was added to dilute the labeled cRNA.

    Mass Spectrometry:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix. .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Genome Wide:

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: Paragraph title: Human genome-wide microarray ... The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Hybridization:

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA labeling and array hybridization were performed according to a one-color microarray-based gene expression analysis protocol (Agilent) with minor modifications. .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis. .. Arraystar Human LncRNA microarray v3.0 designed for global profiling of human LncRNAs (32,586) and protein-coding transcripts (26,109) covering all entries of Refseq, UCSC known genes and Gencode was employed.

    High Performance Liquid Chromatography:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix. .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Flow Cytometry:

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: The purity of RNA samples was assessed using the ND-1000 Nanodrop. .. The purified RNAs were subsequently subjected to first-strand and second-strand cDNA synthesis, followed by adapter ligation and enrichment with a low cycle according to the instructions from TruSeq® RNA LT/HT Sample Prep Kit (Illumina, USA).

    Ligation:

    Article Title: Alcohol consumption promotes colorectal carcinoma metastasis via a CCL5-induced and AMPK-pathway-mediated activation of autophagy
    Article Snippet: RNA purity was assessed using the ND-1000 Nanodrop. .. Briefly, mRNAs were isolated from Total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (illumina, USA) and fragmented to approximately 200 bp.

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: The purity of RNA samples was assessed using the ND-1000 Nanodrop. .. The purity of RNA samples was assessed using the ND-1000 Nanodrop.

    Cell Culture:

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: Total RNA from placental tissues and cultured cells were extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. .. The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels.

    Article Title: Type 2 diabetes and obesity induce similar transcriptional reprogramming in human myocytes
    Article Snippet: In vitro myocytes, cultured as described above (but excluding insulin stimulation), from an additional set of 12 male subjects (from the same larger cohort as the 24 main subjects, described above) were used for follow-up analysis using quantitative real-time PCR (qPCR). .. Total RNA was dissolved in RNase-free water and quantified using a Nanodrop ND 1000 (Saveen biotech ApS, Arhus, Denmark).

    Generated:

    Article Title: Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development
    Article Snippet: The concentration of each sample is determined by NanoDrop ND-1000. .. Final cDNA concentration and dye-incorporation was assessed on a NanoDrop ND-1000 spectrophotometer.

    Polymerase Chain Reaction:

    Article Title: Autoimmune Gastro-Pancreatitis with Anti-Protein Disulfide Isomerase-Associated 2 Autoantibody in Aire-Deficient BALB/cAnN Mice
    Article Snippet: Total RNA from thymus and pancreas were prepared using ISOGEN (NIPPON GENE, Tokyo, Japan) and the concentration of total RNA was measured by NanoDrop ND-1000 (LMS, Tokyo, Japan). .. Total RNA from thymus and pancreas were prepared using ISOGEN (NIPPON GENE, Tokyo, Japan) and the concentration of total RNA was measured by NanoDrop ND-1000 (LMS, Tokyo, Japan).

    Fluorescence:

    Article Title: Molecular profiling of single circulating tumor cells with diagnostic intention
    Article Snippet: Then, each WGA product is labeled with fluorescence dye-conjugated dCTP and dUTP by two independent PCRs (Cy3 for test and Cy5 for reference sample). .. DNA yields and dye incorporation rates were quantified using the NanoDrop ND-1000.

    Isolation:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: The integrity and quantity of each total RNA samples extracted from primary MSCs was examined using agarose gel electrophoresis and Nanodrop™ instrument. mRNA was isolated from total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (For next generation sequencing using). .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Article Title: Polytherapy with a combination of three repurposed drugs (PXT3003) down-regulates Pmp22 over-expression and improves myelination, axonal and functional parameters in models of CMT1A neuropathy
    Article Snippet: Total RNA was isolated from RT4 cells using the RNeasy Micro Kit (Qiagen Gmbh) as described by the manufacturer’s protocol (Qiagen-RNeasy Micro Handbook), while total RNA was isolated from sciatic nerves using Qiazol (Qiagen Gmbh) followed by the single-step purification method with RNeasy Mini Kit (Qiagen Gmbh) as described by the manufacturer’s protocol (Qiagen-RNeasy Fibrous tissue Handbook). .. RNA concentrations were estimated by NanoDrop ND-1000.

    Article Title: Type 2 diabetes and obesity induce similar transcriptional reprogramming in human myocytes
    Article Snippet: Paragraph title: Additional subjects, RNA isolation, and quantitative PCR ... Total RNA was dissolved in RNase-free water and quantified using a Nanodrop ND 1000 (Saveen biotech ApS, Arhus, Denmark).

    Article Title: IL-20 receptor signaling inhibits cutaneous IL-1? and IL-17A production to promote methicillin-resistant Staphylococcus aureus infection
    Article Snippet: Paragraph title: RNA Isolation ... RNA yield was measured on a NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: Total RNAs from experimental samples were isolated using the RNeasy plus mini kit (Qiagen), incorporating on-column DNase treatment using the RNase-Free DNase Set. .. RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis.

    Article Title: Alcohol consumption promotes colorectal carcinoma metastasis via a CCL5-induced and AMPK-pathway-mediated activation of autophagy
    Article Snippet: Total RNA was isolated from cells/tissues using the Trizol (invitrogen) according to the manufacturer’s protocol. .. RNA purity was assessed using the ND-1000 Nanodrop.

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: Briefly, total RNA was isolated from bacteria using the Trizol (Invitrogen) according to the manufacture’s protocol. .. The purity of RNA samples was assessed using the ND-1000 Nanodrop.

    Labeling:

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: The labeled cRNAs were purified by RNeasy Mini kit (Qiagen). .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. .. One microgram of each labeled cRNA was fragmented by adding 5 μl 10 × blocking agent and 1 μl of 25 × fragmentation buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE hybridization buffer was added to dilute the labeled cRNA.

    Article Title: Molecular profiling of single circulating tumor cells with diagnostic intention
    Article Snippet: The two labeling reactions (test and reference) are pooled and purified using Amicon Ultra 0.5 columns. .. DNA yields and dye incorporation rates were quantified using the NanoDrop ND-1000.

    Article Title: Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development
    Article Snippet: The concentration of each sample is determined by NanoDrop ND-1000. .. Final cDNA concentration and dye-incorporation was assessed on a NanoDrop ND-1000 spectrophotometer.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis. .. RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis.

    Purification:

    Article Title: Inference and quantification of peptidoforms in large sample cohorts by SWATH-MS
    Article Snippet: Digests were combined and purified with Sep-Pak C18 Vac Cartridge (Waters). .. Peptide amount was determined by using Nanodrop ND-1000 (Thermo Scientific).

    Article Title: Epigenome-wide association studies identify DNA methylation associated with kidney function
    Article Snippet: Genomic DNA (200 ng) was purified using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Purified DNA quality and concentration were assessed with NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) and by Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA) prior to bisulfite conversion. .. DNA methylation was measured using the Infinium HumanMethylation450 Beadchip (Illumina, San Diego, CA), with 485,000 CpG sites across the genome examined.

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: The integrity and quantity of each total RNA samples extracted from primary MSCs was examined using agarose gel electrophoresis and Nanodrop™ instrument. mRNA was isolated from total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (For next generation sequencing using). .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix. .. Pretreated nucleosides solution was deproteinized using Satorius 10,000-Da MWCO spin filter.

    Article Title: Polytherapy with a combination of three repurposed drugs (PXT3003) down-regulates Pmp22 over-expression and improves myelination, axonal and functional parameters in models of CMT1A neuropathy
    Article Snippet: Total RNA was isolated from RT4 cells using the RNeasy Micro Kit (Qiagen Gmbh) as described by the manufacturer’s protocol (Qiagen-RNeasy Micro Handbook), while total RNA was isolated from sciatic nerves using Qiazol (Qiagen Gmbh) followed by the single-step purification method with RNeasy Mini Kit (Qiagen Gmbh) as described by the manufacturer’s protocol (Qiagen-RNeasy Fibrous tissue Handbook). .. RNA concentrations were estimated by NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: The labeled cRNAs were purified by RNeasy Mini kit (Qiagen). .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Article Title: Molecular profiling of single circulating tumor cells with diagnostic intention
    Article Snippet: The two labeling reactions (test and reference) are pooled and purified using Amicon Ultra 0.5 columns. .. DNA yields and dye incorporation rates were quantified using the NanoDrop ND-1000.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis. .. RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis.

    Article Title: Alcohol consumption promotes colorectal carcinoma metastasis via a CCL5-induced and AMPK-pathway-mediated activation of autophagy
    Article Snippet: RNA purity was assessed using the ND-1000 Nanodrop. .. RNA purity was assessed using the ND-1000 Nanodrop.

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: The purity of RNA samples was assessed using the ND-1000 Nanodrop. .. The purity of RNA samples was assessed using the ND-1000 Nanodrop.

    Sequencing:

    Article Title: Inference and quantification of peptidoforms in large sample cohorts by SWATH-MS
    Article Snippet: The samples were then resolved by 100mM NH4 HCO3 and were digested with sequencing-grade porcine trypsin (Promega) at a protease/protein ratio of 1:50 overnight at 37 °C . .. Peptide amount was determined by using Nanodrop ND-1000 (Thermo Scientific).

    Article Title: Alcohol consumption promotes colorectal carcinoma metastasis via a CCL5-induced and AMPK-pathway-mediated activation of autophagy
    Article Snippet: Paragraph title: High-throughput sequencing and bioinformatics analysis ... RNA purity was assessed using the ND-1000 Nanodrop.

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: Paragraph title: Drug resistance study and gene sequencing ... The purity of RNA samples was assessed using the ND-1000 Nanodrop.

    Agarose Gel Electrophoresis:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: The integrity and quantity of each total RNA samples extracted from primary MSCs was examined using agarose gel electrophoresis and Nanodrop™ instrument. mRNA was isolated from total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (For next generation sequencing using). .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: Total RNAs from experimental samples were isolated using the RNeasy plus mini kit (Qiagen), incorporating on-column DNase treatment using the RNase-Free DNase Set. .. RNA quantity and quality were assessed by a NanoDrop ND-1000, with RNA integrity determined by standard denaturing agarose gel electrophoresis. .. Arraystar Human LncRNA microarray v3.0 designed for global profiling of human LncRNAs (32,586) and protein-coding transcripts (26,109) covering all entries of Refseq, UCSC known genes and Gencode was employed.

    Liquid Chromatography:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: Paragraph title: LC-MS/MS analysis of m6 A level ... Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Software:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix. .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

    Article Title: Molecular profiling of single circulating tumor cells with diagnostic intention
    Article Snippet: DNA yields and dye incorporation rates were quantified using the NanoDrop ND-1000. .. Array CGH was performed on oligonucleotide-based SurePrint G3 Human CGH 4 × 180K microarray slides (design code: 022060) according the protocol provided by the manufacturer (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, version 7.1, December 2011) with slight modifications (Czyz et al , ).

    SYBR Green Assay:

    Article Title: Polytherapy with a combination of three repurposed drugs (PXT3003) down-regulates Pmp22 over-expression and improves myelination, axonal and functional parameters in models of CMT1A neuropathy
    Article Snippet: RNA concentrations were estimated by NanoDrop ND-1000. .. 80 ng of total RNA was reverse transcribed using SuperScript™ II Reverse Transcriptase with Oligo(dT)12–18 (Invitrogen), then, qPCR was performed with a rapid thermal cycler system (LightCycler® 480 II, Roche).

    RNA Extraction:

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: Paragraph title: 2.2. RNA extraction, miRNA microarray analysis, and quantitative real-time PCR (qRT-PCR) ... The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels.

    Positron Emission Tomography:

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: After treatment with pEt_20 or imipenem for 30 passages, the RNA samples of the bacteria at 30 passages and the first passage were extracted, sequenced, and analyzed. .. The purity of RNA samples was assessed using the ND-1000 Nanodrop.

    Sample Prep:

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: The purity of RNA samples was assessed using the ND-1000 Nanodrop. .. The purity of RNA samples was assessed using the ND-1000 Nanodrop.

    In Vitro:

    Article Title: Type 2 diabetes and obesity induce similar transcriptional reprogramming in human myocytes
    Article Snippet: In vitro myocytes, cultured as described above (but excluding insulin stimulation), from an additional set of 12 male subjects (from the same larger cohort as the 24 main subjects, described above) were used for follow-up analysis using quantitative real-time PCR (qPCR). .. Total RNA was dissolved in RNase-free water and quantified using a Nanodrop ND 1000 (Saveen biotech ApS, Arhus, Denmark).

    Electrophoresis:

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: To prevent RNA degradation, all steps were performed on ice. .. The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels. .. RNA was reverse transcribed using the specific Megaplex RT primers (Life Technologies).

    Next-Generation Sequencing:

    Article Title: Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis
    Article Snippet: The integrity and quantity of each total RNA samples extracted from primary MSCs was examined using agarose gel electrophoresis and Nanodrop™ instrument. mRNA was isolated from total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (For next generation sequencing using). .. Purified mRNA was quantified using NanoDrop ND-1000. mRNA was hydrolyzed to single nucleosides and then nucleosides were dephosphorylated by enzyme mix.

    Spectrophotometry:

    Article Title: Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria
    Article Snippet: This work was supported by the Swedish Energy Agency, the Knut and Alice Wallenberg Foundation, the Nordic Energy Research Program (project BioH2), the EU/NEST project BioModularH2 (contract # 043340), and the EU/Energy project SOLAR-H2 (contract # 212508). .. The authors would like to thank Elin Övernäs and Marie Englund, from the Department of Evolution, Genomics and Systematics, Uppsala University, for access to, and help with the use of the NanoDrop ND-1000 UV/Vis spectrophotometer. ..

    Article Title: Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria
    Article Snippet: For the "PGTX" protocols, Trizol was simply replaced by the disruption mixture we propose in this paper. .. RNA concentration and purity was measured using the NanoDrop ND-1000 UV/Vis spectrophotometer according to manufacturer's instructions (NanoDrop Technologies, USA). .. RNA integrity was verified using Biorad's automated electrophoresis system Experion (RNA StdSens analysis kit), according to manufacturer's instructions.

    Article Title: Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development
    Article Snippet: The concentration of each sample is determined by NanoDrop ND-1000. .. The concentration of each sample is determined by NanoDrop ND-1000.

    DNA Methylation Assay:

    Article Title: Epigenome-wide association studies identify DNA methylation associated with kidney function
    Article Snippet: Paragraph title: Kidney tissue cohort and DNA methylation quantification ... Purified DNA quality and concentration were assessed with NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) and by Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA) prior to bisulfite conversion.

    Concentration Assay:

    Article Title: Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria
    Article Snippet: For the "PGTX" protocols, Trizol was simply replaced by the disruption mixture we propose in this paper. .. RNA concentration and purity was measured using the NanoDrop ND-1000 UV/Vis spectrophotometer according to manufacturer's instructions (NanoDrop Technologies, USA). .. RNA integrity was verified using Biorad's automated electrophoresis system Experion (RNA StdSens analysis kit), according to manufacturer's instructions.

    Article Title: Autoimmune Gastro-Pancreatitis with Anti-Protein Disulfide Isomerase-Associated 2 Autoantibody in Aire-Deficient BALB/cAnN Mice
    Article Snippet: The data were recorded as the absorbance at 490 nm corrected by a background and a reference wavelength of 655 nm. .. Total RNA from thymus and pancreas were prepared using ISOGEN (NIPPON GENE, Tokyo, Japan) and the concentration of total RNA was measured by NanoDrop ND-1000 (LMS, Tokyo, Japan). .. PCR was carried out on cDNA prepared using High-Capacity cDNA Reverse Transcription kit (Life Technologies, Tokyo, Japan) from 1 µg RNA.

    Article Title: Epigenome-wide association studies identify DNA methylation associated with kidney function
    Article Snippet: Genomic DNA (200 ng) was purified using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Purified DNA quality and concentration were assessed with NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) and by Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA) prior to bisulfite conversion. .. DNA methylation was measured using the Infinium HumanMethylation450 Beadchip (Illumina, San Diego, CA), with 485,000 CpG sites across the genome examined.

    Article Title: Elevated microRNA-141-3p in placenta of non-diabetic macrosomia regulate trophoblast proliferation
    Article Snippet: To prevent RNA degradation, all steps were performed on ice. .. The concentration and purity of RNA were measured using NanoDrop® ND-1000 (samples with A260/A280 ratio > 1·8 and < 2·0 were stored at −80 °C until use), while its integrity was assessed using electrophoresis with 1·5% denaturing agarose gels. .. RNA was reverse transcribed using the specific Megaplex RT primers (Life Technologies).

    Article Title: The senescence-associated secretory phenotype is potentiated by feedforward regulatory mechanisms involving Zscan4 and TAK1
    Article Snippet: The labeled cRNAs were purified by RNeasy Mini kit (Qiagen). .. The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. .. One microgram of each labeled cRNA was fragmented by adding 5 μl 10 × blocking agent and 1 μl of 25 × fragmentation buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE hybridization buffer was added to dilute the labeled cRNA.

    Article Title: Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development
    Article Snippet: Total RNA, unbound, and bound fractions of mRNA are precipitated by traditional methods (0.1 vol NaCl, 0.1 vol linear acrylamide and 3× volume 100% EtOH) followed by resuspension in 20 μL DEPC-treated water. .. The concentration of each sample is determined by NanoDrop ND-1000. .. Starting with 1.0–2.5 μg of RNA, single-strand aminoallyl-containing cDNA synthesis and Amersham CyDye-coupling (GE Healthcare) was carried out as previously described with the addition of control RNAs to aid in the normalization between samples and arrays (two color RNA spike-in kit, Agilent Technologies).

    In Situ:

    Article Title: A macromolecular approach to eradicate multidrug resistant bacterial infections while mitigating drug resistance onset
    Article Snippet: The purity of RNA samples was assessed using the ND-1000 Nanodrop. .. The purified RNAs were subsequently subjected to first-strand and second-strand cDNA synthesis, followed by adapter ligation and enrichment with a low cycle according to the instructions from TruSeq® RNA LT/HT Sample Prep Kit (Illumina, USA).

    High Throughput Screening Assay:

    Article Title: Alcohol consumption promotes colorectal carcinoma metastasis via a CCL5-induced and AMPK-pathway-mediated activation of autophagy
    Article Snippet: Paragraph title: High-throughput sequencing and bioinformatics analysis ... RNA purity was assessed using the ND-1000 Nanodrop.

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    Thermo Fisher nd 1000 spectrophotometer
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