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Thermo Fisher nd 1000
Nd 1000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nd 1000 - by Bioz Stars, 2020-02
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Centrifugation:

Article Title: The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction
Article Snippet: The cell-free media containing secreted proteins was filtered through an Amicon® Ultra-4 3K Centrifugal Filter Unit (Merck Millipore, Darmstadt, Germany) and then collected by centrifugation at 959× g for 75 min [ ]. .. The protein concentration was measured with an ND-1000 (NanoDrop, Thermo, Waltham, MA, USA) and a protein assay (Bio-Rad, Hercules, CA, USA).

Article Title: Harmonization of pipeline for preclinical multicenter plasma protein and miRNA biomarker discovery in a rat model of post-traumatic epileptogenesis
Article Snippet: .. Following centrifugation , the supernatant (i.e., plasma) within the sampling tube was assessed for degree of hemolysis by measuring UV-Vis absorbance of hemoglobin at 414 nm (ND-1000, NanoDrop™ , Thermo Fisher Scientific, Wilmington, DE). ..

Amplification:

Article Title: Specific role of N-methyl-D-aspartate (NMDA) receptor in elastin-derived VGVAPG peptide-dependent calcium homeostasis in mouse cortical astrocytes in vitro
Article Snippet: Both the quality and quantity of the RNA were determined spectrophotometrically at 260 and 280 nm, respectively (ND/1000 UV/Vis; Thermo Fisher NanoDrop, USA). .. Products from the RT reaction were amplified using the FastStart Universal Probe Master (Rox) kit with TaqMan probes as primers for specific genes encoding Actb, Glb1, GluN1, GluN2A , and GluN2B .

Synthesized:

Article Title: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling
Article Snippet: RNA concentration was quantified (ND-1000, Nanodrop). .. First strand cDNA was synthesized (iScript, Biorad) from 500 ng of total RNA for Taqman (Life Technologies) probes Aggrecan (Mm00545794_m1), AdamtS5 (Mm00478620_m1), b-Catenin (Mm01350387_g1, Mm00483029_g1), Col1a (00801666_g1), Col2a (Mm001309565_m1), MMP13 (Mm00439491_m1), DKK1 (Mm00438422_m1), CCND1 (Mm00432359_m1), Brachyury (T) (Mm00436877_m1), osterix (Mm04209856), Axin2 (MM00443610), Sun1 (Mm00659179_m1), Sun2 (Mm01299500_m1), Nesp1 (Hs00326979_m1), Nesp2 (Hs00794881_m1) and Wnt ligands (1: Mm01300555, 2: Mm00470018, 2b: Mm00437330, 3a: Mm00437337, 3: Mm00437336, 4: Mm01194003, 5a: Mm00437347, 5b: Mm01183986, 6: Mm00437353, 7a: Mm00437356, 7b: Mm01301717, 8a: Mm01157914, 8b: Mm00442108, 9a: Mm00460518, 9b: Mm00457102, 10a: Mm00437325, 10b: Mm00442104, 11: Mm00437327, 16: Mm00446420).

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA. .. The primers in the reverse transcription-quantitative PCR (RT-qPCR) were designed and synthesized by the Takara Biotechnology Ltd., using Premier 5.0 software.

Quantitative RT-PCR:

Article Title: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling
Article Snippet: Paragraph title: qRT-PCR ... RNA concentration was quantified (ND-1000, Nanodrop).

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: .. RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: Paragraph title: Quantitative RT-PCR analysis. ... The quantity and quality of the isolated RNA was determined spectrophotometrically (ND-1000, NanoDrop Technologies, Wilmington, DE) as previously described ( , ).

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA. .. The primers in the reverse transcription-quantitative PCR (RT-qPCR) were designed and synthesized by the Takara Biotechnology Ltd., using Premier 5.0 software.

Real-time Polymerase Chain Reaction:

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Specific role of N-methyl-D-aspartate (NMDA) receptor in elastin-derived VGVAPG peptide-dependent calcium homeostasis in mouse cortical astrocytes in vitro
Article Snippet: Paragraph title: Real-time PCR analysis of mRNAs specific to genes encoding GluN1, GluN2A , and GluN2B ... Both the quality and quantity of the RNA were determined spectrophotometrically at 260 and 280 nm, respectively (ND/1000 UV/Vis; Thermo Fisher NanoDrop, USA).

Microarray:

Article Title: The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Article Snippet: Paragraph title: 3.10. Microarray Analysis ... The quality of the RNA samples were determined using a ND-1000 (NanoDrop Technologies, Thermo Scientific, Waltham, USA) and by agarose gel electrophoresis.

Incubation:

Article Title: Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin
Article Snippet: Before RNA extraction, frozen tissues were incubated in RNA later ICE (Invitrogen) solution over night at 4℃. .. RNA quantities and qualities were determined using UV spectrophotometer at 260 nm (ND-1000, Nano Drop Technologies, Inc.).

Expressing:

Article Title: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling
Article Snippet: RNA concentration was quantified (ND-1000, Nanodrop). .. The relative gene expression in each loaded intervertebral disc was represented by normalizing to Ipo8 (Mm01255158_m1) and then normalized to the control intervertebral disc (2−∆∆CT ) or to the average WT value.

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: The quantity and quality of the isolated RNA was determined spectrophotometrically (ND-1000, NanoDrop Technologies, Wilmington, DE) as previously described ( , ). .. Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) was carried out using SYBR gene expression master mixes (Applied Biosystems).

Sampling:

Article Title: Harmonization of pipeline for preclinical multicenter plasma protein and miRNA biomarker discovery in a rat model of post-traumatic epileptogenesis
Article Snippet: .. Following centrifugation , the supernatant (i.e., plasma) within the sampling tube was assessed for degree of hemolysis by measuring UV-Vis absorbance of hemoglobin at 414 nm (ND-1000, NanoDrop™ , Thermo Fisher Scientific, Wilmington, DE). ..

Hybridization:

Article Title: The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Article Snippet: The quality of the RNA samples were determined using a ND-1000 (NanoDrop Technologies, Thermo Scientific, Waltham, USA) and by agarose gel electrophoresis. .. The cDNA synthesis, labeling and hybridization experiments were carried out as described previously [ ].

Transfection:

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: Reverse transcription-quantitative PCR For 48 h after transfection, the trophoblast cells in each group were treated with lysis buffer (Beijing Ovia Bio) and placed on ice for lysis. .. Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA.

Cell Culture:

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: .. RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Specific role of N-methyl-D-aspartate (NMDA) receptor in elastin-derived VGVAPG peptide-dependent calcium homeostasis in mouse cortical astrocytes in vitro
Article Snippet: For the real-time PCR assay, astrocytes were seeded onto 12-well plates and initially cultured for 24 h. After 6-h exposure to 10 nM VGVAPG peptide, the samples were collected, and total RNA was extracted from the astrocytes using a Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. .. Both the quality and quantity of the RNA were determined spectrophotometrically at 260 and 280 nm, respectively (ND/1000 UV/Vis; Thermo Fisher NanoDrop, USA).

Protein Concentration:

Article Title: The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction
Article Snippet: .. The protein concentration was measured with an ND-1000 (NanoDrop, Thermo, Waltham, MA, USA) and a protein assay (Bio-Rad, Hercules, CA, USA). .. Cytopathic Effect Assay (CPE) and Giemsa Stain Human primary corneal epithelial cells were axenically cultured in an EpiGRO™ Human Epidermal Keratinocyte Complete Culture Media Kit (Merck Millipore, Darmstadt, Germany).

Polymerase Chain Reaction:

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: .. RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: The quantity and quality of the isolated RNA was determined spectrophotometrically (ND-1000, NanoDrop Technologies, Wilmington, DE) as previously described ( , ). .. The miR-29 primer design and PCR conditions have been described previously ( ).

Article Title: Differential Gene Expression During Compensatory Sprouting of Dendrites in the Auditory System of the Cricket Gryllus bimaculatus
Article Snippet: RNA was purified using RNApure Reagent (GenHunter), DNaseI-treated with the MessageClean Kit (GenHunter), and quantified by an ND-1000 (nanodrop) Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. The first-strand synthesis reaction was diluted in order to allow reproducible and accurate aliquots (5 μl) to be removed for subsequent PCR reactions ( ).

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: Paragraph title: Reverse transcription-quantitative PCR ... Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA.

Methylation:

Article Title: Glutathione Deficiency and Alterations in the Sulfur Amino Acid Homeostasis during Early Postnatal Development as Potential Triggering Factors for Schizophrenia-Like Behavior in Adult Rats
Article Snippet: Global changes in DNA methylation were measured in the tested tissue samples using a specific ELISA-based Kit (Imprint® Methylated DNA Quantification). .. The quantity and quality of DNA was spectrophotometrically measured at 260 nm and 260/280 nm (ND/1000 UV/VIS; Thermo Fisher NanoDrop, Waltham, MA, USA) [ , ].

Isolation:

Article Title: The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction
Article Snippet: Paragraph title: 4.3. Isolation of Secreted Proteins ... The protein concentration was measured with an ND-1000 (NanoDrop, Thermo, Waltham, MA, USA) and a protein assay (Bio-Rad, Hercules, CA, USA).

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: .. RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: .. The quantity and quality of the isolated RNA was determined spectrophotometrically (ND-1000, NanoDrop Technologies, Wilmington, DE) as previously described ( , ). .. An RNA sample of 1 μg each was reverse transcribed using primers for collagen type 1 (COL1A1) (Fitzgerald Industries, Acton, MA), collagen 3A1 (COL3A1) (Proteintech Group, Chicago, IL), collagen 4A5 (COL4A5) (Proteintech Group), and ELN (Santa Cruz Biotechnology, Dallas, TX).

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis
Article Snippet: Paragraph title: 4.2. RNA Isolation and Complementary DNA (cDNA) Synthesis ... The RNA concentration was measured spectroscopically (ND 1000; NanoDrop Technologies, Wilmington, DE, USA).

Article Title: The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Article Snippet: DMSO (untreated control) for 1 h and 6 h were obtained and total RNA was isolated using the Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions. .. The quality of the RNA samples were determined using a ND-1000 (NanoDrop Technologies, Thermo Scientific, Waltham, USA) and by agarose gel electrophoresis.

Labeling:

Article Title: The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Article Snippet: The quality of the RNA samples were determined using a ND-1000 (NanoDrop Technologies, Thermo Scientific, Waltham, USA) and by agarose gel electrophoresis. .. The cDNA synthesis, labeling and hybridization experiments were carried out as described previously [ ].

Purification:

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: .. RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Detection of Mycobacterium avium subsp. paratuberculosis by a Sonicate Immunoassay Based on Surface-Enhanced Raman Scattering ▿
Article Snippet: Three monoclonal antibodies (8G2, 13E1, and 12C9, with the former two being specific for the MAP2121c protein) were tested for performance after purification using Melon gel (Pierce, Rockford, IL). .. The concentrations of the mAb solutions were determined spectrophotometrically (ND-1000, using a standard mass extinction coefficient of 13.7 liters g−1 cm−1 for 10 mg/ml immunoglobulin G solution as a reference); all mAb dilutions employed 50 mM borate buffer (pH 8.3 borate buffer packs; Pierce).

Article Title: Differential Gene Expression During Compensatory Sprouting of Dendrites in the Auditory System of the Cricket Gryllus bimaculatus
Article Snippet: .. RNA was purified using RNApure Reagent (GenHunter), DNaseI-treated with the MessageClean Kit (GenHunter), and quantified by an ND-1000 (nanodrop) Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. Single-stranded cDNA was made from 3 μg total RNA using the Superscript III First-Strand Synthesis System for RT-PCR (Invitrogen).

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis
Article Snippet: RNA Isolation and Complementary DNA (cDNA) Synthesis Frozen samples of SM were homogenized with Speedmill Plus (Analytik Jena AG, Jena, Germany) and total RNA from healthy, OA, and RA samples of human SM was purified with isopropanol and repeated ethanol precipitation and isolated with Superscript™ II-RT-Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) using the following protocol: Contaminating DNA was eliminated by digestion of RNAse free DNAse I (30 min at 37 °C). .. The RNA concentration was measured spectroscopically (ND 1000; NanoDrop Technologies, Wilmington, DE, USA).

Article Title: The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Article Snippet: The quality of the RNA samples were determined using a ND-1000 (NanoDrop Technologies, Thermo Scientific, Waltham, USA) and by agarose gel electrophoresis. .. The amino-allyl cDNA was then purified and concentrated using the Zymo DNA clean and concentrator-5 column (Zymo Research, California, USA).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: The quantity and quality of the isolated RNA was determined spectrophotometrically (ND-1000, NanoDrop Technologies, Wilmington, DE) as previously described ( , ). .. Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) was carried out using SYBR gene expression master mixes (Applied Biosystems).

Article Title: Differential Gene Expression During Compensatory Sprouting of Dendrites in the Auditory System of the Cricket Gryllus bimaculatus
Article Snippet: Paragraph title: Semi-quantitative RT-PCR ... RNA was purified using RNApure Reagent (GenHunter), DNaseI-treated with the MessageClean Kit (GenHunter), and quantified by an ND-1000 (nanodrop) Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA).

Article Title: Specific role of N-methyl-D-aspartate (NMDA) receptor in elastin-derived VGVAPG peptide-dependent calcium homeostasis in mouse cortical astrocytes in vitro
Article Snippet: Both the quality and quantity of the RNA were determined spectrophotometrically at 260 and 280 nm, respectively (ND/1000 UV/Vis; Thermo Fisher NanoDrop, USA). .. Two-step real-time reverse transcription (RT)-PCR was conducted with both the RT reaction and the quantitative PCR (qPCR) run using the CFX Real Time System (BioRad, USA).

Software:

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA. .. The primers in the reverse transcription-quantitative PCR (RT-qPCR) were designed and synthesized by the Takara Biotechnology Ltd., using Premier 5.0 software.

Enzyme-linked Immunosorbent Assay:

Article Title: Glutathione Deficiency and Alterations in the Sulfur Amino Acid Homeostasis during Early Postnatal Development as Potential Triggering Factors for Schizophrenia-Like Behavior in Adult Rats
Article Snippet: Global changes in DNA methylation were measured in the tested tissue samples using a specific ELISA-based Kit (Imprint® Methylated DNA Quantification). .. The quantity and quality of DNA was spectrophotometrically measured at 260 nm and 260/280 nm (ND/1000 UV/VIS; Thermo Fisher NanoDrop, Waltham, MA, USA) [ , ].

RNA Extraction:

Article Title: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling
Article Snippet: Total RNA extraction was performed using a standard kit (RNeasy mini kit, Qiagen). .. RNA concentration was quantified (ND-1000, Nanodrop).

Article Title: Functional impact of the long non-coding RNA MEG3 deletion by CRISPR/Cas9 in the human triple negative metastatic Hs578T cancer cell line
Article Snippet: .. RNA extraction, cDNA synthesis and reverse transcription-quantitative PCR (RT-qPCR) Total RNA was isolated from cultured cells with Trizol (Life Technologies) and purified with an RNAspin Mini kit (GE Healthcare) according to the manufacturer's instructions, with an extended treatment with DNase I for 1 h. Total RNA was quantified using the ND-1000 (NanoDrop) and its integrity was assessed on a Bioanalyzer (Agilent Technologies). .. For measuring lncRNAs, reverse transcription was performed with the SuperScript III (Life Technologies) followed by qPCR.

Article Title: Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin
Article Snippet: Before RNA extraction, frozen tissues were incubated in RNA later ICE (Invitrogen) solution over night at 4℃. .. RNA quantities and qualities were determined using UV spectrophotometer at 260 nm (ND-1000, Nano Drop Technologies, Inc.).

Agarose Gel Electrophoresis:

Article Title: The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Article Snippet: .. The quality of the RNA samples were determined using a ND-1000 (NanoDrop Technologies, Thermo Scientific, Waltham, USA) and by agarose gel electrophoresis. .. The cDNA synthesis, labeling and hybridization experiments were carried out as described previously [ ].

Ethanol Precipitation:

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis
Article Snippet: RNA Isolation and Complementary DNA (cDNA) Synthesis Frozen samples of SM were homogenized with Speedmill Plus (Analytik Jena AG, Jena, Germany) and total RNA from healthy, OA, and RA samples of human SM was purified with isopropanol and repeated ethanol precipitation and isolated with Superscript™ II-RT-Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) using the following protocol: Contaminating DNA was eliminated by digestion of RNAse free DNAse I (30 min at 37 °C). .. The RNA concentration was measured spectroscopically (ND 1000; NanoDrop Technologies, Wilmington, DE, USA).

Spectrophotometry:

Article Title: Differential Gene Expression During Compensatory Sprouting of Dendrites in the Auditory System of the Cricket Gryllus bimaculatus
Article Snippet: .. RNA was purified using RNApure Reagent (GenHunter), DNaseI-treated with the MessageClean Kit (GenHunter), and quantified by an ND-1000 (nanodrop) Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. Single-stranded cDNA was made from 3 μg total RNA using the Superscript III First-Strand Synthesis System for RT-PCR (Invitrogen).

Article Title: Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin
Article Snippet: .. RNA quantities and qualities were determined using UV spectrophotometer at 260 nm (ND-1000, Nano Drop Technologies, Inc.). .. Total RNA (1 µg) was used to generate cDNA using SuperScript VILO reverse transcriptase kit (Invitrogen).

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: .. Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA. .. The GoScript™ Sensiscript RT kit (A5004; Promega Corp.) was used to reversely transcribe the RNA into cDNA.

DNA Methylation Assay:

Article Title: Glutathione Deficiency and Alterations in the Sulfur Amino Acid Homeostasis during Early Postnatal Development as Potential Triggering Factors for Schizophrenia-Like Behavior in Adult Rats
Article Snippet: Paragraph title: 4.3.5. Measurement of the Global DNA Methylation ... The quantity and quality of DNA was spectrophotometrically measured at 260 nm and 260/280 nm (ND/1000 UV/VIS; Thermo Fisher NanoDrop, Waltham, MA, USA) [ , ].

Produced:

Article Title: Detection of Mycobacterium avium subsp. paratuberculosis by a Sonicate Immunoassay Based on Surface-Enhanced Raman Scattering ▿
Article Snippet: Monoclonal antibodies (mAbs) to MAP2121c, an M. avium subsp. paratuberculosis surface protein, have recently been produced ( ). .. The concentrations of the mAb solutions were determined spectrophotometrically (ND-1000, using a standard mass extinction coefficient of 13.7 liters g−1 cm−1 for 10 mg/ml immunoglobulin G solution as a reference); all mAb dilutions employed 50 mM borate buffer (pH 8.3 borate buffer packs; Pierce).

Concentration Assay:

Article Title: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling
Article Snippet: .. RNA concentration was quantified (ND-1000, Nanodrop). .. First strand cDNA was synthesized (iScript, Biorad) from 500 ng of total RNA for Taqman (Life Technologies) probes Aggrecan (Mm00545794_m1), AdamtS5 (Mm00478620_m1), b-Catenin (Mm01350387_g1, Mm00483029_g1), Col1a (00801666_g1), Col2a (Mm001309565_m1), MMP13 (Mm00439491_m1), DKK1 (Mm00438422_m1), CCND1 (Mm00432359_m1), Brachyury (T) (Mm00436877_m1), osterix (Mm04209856), Axin2 (MM00443610), Sun1 (Mm00659179_m1), Sun2 (Mm01299500_m1), Nesp1 (Hs00326979_m1), Nesp2 (Hs00794881_m1) and Wnt ligands (1: Mm01300555, 2: Mm00470018, 2b: Mm00437330, 3a: Mm00437337, 3: Mm00437336, 4: Mm01194003, 5a: Mm00437347, 5b: Mm01183986, 6: Mm00437353, 7a: Mm00437356, 7b: Mm01301717, 8a: Mm01157914, 8b: Mm00442108, 9a: Mm00460518, 9b: Mm00457102, 10a: Mm00437325, 10b: Mm00442104, 11: Mm00437327, 16: Mm00446420).

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis
Article Snippet: .. The RNA concentration was measured spectroscopically (ND 1000; NanoDrop Technologies, Wilmington, DE, USA). .. This procedure was followed by performing cDNA synthesis using RevertAid H Minus reverse transcriptase (Thermo Fisher Scientific Inc., Waltham, MA, USA) and following a standard protocol. β-actin was used as an internal control to ensure the integrity of cDNA.

Marker:

Article Title: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling
Article Snippet: RNA concentration was quantified (ND-1000, Nanodrop). .. Brachyury (notochordal marker T) expression was used as Wnt signaling indicator because it requires TCF/LEF signaling and notochordal fate is driven by Wnt signaling .

Lysis:

Article Title: miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9
Article Snippet: Reverse transcription-quantitative PCR For 48 h after transfection, the trophoblast cells in each group were treated with lysis buffer (Beijing Ovia Bio) and placed on ice for lysis. .. Subsequently, the total RNA was dissolved in ultrapure water treated with diethylpyrocarbonate (E174-5G; Shanghai Haoran Biotechnology Co., Ltd.) and the optical density at 260 and 280 nm was measured using ND-1000 UV/Vis spectrophotometer (Evolution 201; Thermo Fisher Scientific, Inc.) in order to determine the quality of RNA.

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    Thermo Fisher nano drop 1000 spectrophotometer
    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by <t>Nano-drop</t> 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P
    Nano Drop 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop 1000 spectrophotometer/product/Thermo Fisher
    Average 90 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    nano drop 1000 spectrophotometer - by Bioz Stars, 2020-02
    90/100 stars
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    99
    Thermo Fisher nanodrop spectrophotometer nd 1000
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nanodrop Spectrophotometer Nd 1000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop spectrophotometer nd 1000/product/Thermo Fisher
    Average 99 stars, based on 466 article reviews
    Price from $9.99 to $1999.99
    nanodrop spectrophotometer nd 1000 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher nanodroptm nd 1000
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nanodroptm Nd 1000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodroptm nd 1000/product/Thermo Fisher
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    nanodroptm nd 1000 - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    Image Search Results


    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Journal: MethodsX

    Article Title: Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction

    doi: 10.1016/j.mex.2019.10.015

    Figure Lengend Snippet: RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Article Snippet: The yield of total RNA was determined by Nano-drop 1000 Spectrophotometer (Thermo Scientific).

    Techniques: Lysis, Spectrophotometry, RNA Extraction

    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Journal: Veterinary Research

    Article Title: Visualizing bovine leukemia virus (BLV)-infected cells and measuring BLV proviral loads in the milk of BLV seropositive dams

    doi: 10.1186/s13567-019-0724-1

    Figure Lengend Snippet: Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Article Snippet: The quantity and quality of DNA samples extracted from milk sample was determined based on the A260/280 ratio using a Nanodrop Spectrophotometer ND-1000 (Thermo Fisher Scientific).

    Techniques: Infection, Spectrophotometry