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Plasma-based RNA quantification and characterisation (A) Concentration of RNA isolated from plasma specimens, as assessed by NanoDrop <t>ND-1000</t> spectrophotometer. (B(i)) Electropherograms show the presence of 5S and 5.8S subunits, tRNAs and small RNA fragments (
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Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome

Journal: Oncotarget

doi: 10.18632/oncotarget.24314

Plasma-based RNA quantification and characterisation (A) Concentration of RNA isolated from plasma specimens, as assessed by NanoDrop ND-1000 spectrophotometer. (B(i)) Electropherograms show the presence of 5S and 5.8S subunits, tRNAs and small RNA fragments (
Figure Legend Snippet: Plasma-based RNA quantification and characterisation (A) Concentration of RNA isolated from plasma specimens, as assessed by NanoDrop ND-1000 spectrophotometer. (B(i)) Electropherograms show the presence of 5S and 5.8S subunits, tRNAs and small RNA fragments (

Techniques Used: Concentration Assay, Isolation, Spectrophotometry

Related Articles

Centrifugation:

Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome
Article Snippet: RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions. .. RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

Synthesized:

Article Title: Long-distance endosome trafficking drives fungal effector production during plant infection
Article Snippet: In brief, leaf samples were removed 2–4 cm beneath the injection point and harvested from four independent experiments, 30 plants each. .. The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously . .. Putative components of EE-associated signalling were identified by reciprocal BLAST search ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Quantitative RT-PCR:

Article Title: Long-distance endosome trafficking drives fungal effector production during plant infection
Article Snippet: In brief, leaf samples were removed 2–4 cm beneath the injection point and harvested from four independent experiments, 30 plants each. .. The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously . .. Putative components of EE-associated signalling were identified by reciprocal BLAST search ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Article Title: WNK1 kinase and its partners Akt, SGK1 and NBC-family Na+/HCO3− cotransporters are potential therapeutic targets for glioblastoma stem-like cells linked to Bisacodyl signaling
Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment.

Real-time Polymerase Chain Reaction:

Article Title: Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice
Article Snippet: Paragraph title: Quantitative Real-Time PCR Analysis ... The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech, France).

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: Paragraph title: mRNA Expression Analysis by Real-time PCR ... The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France).

Article Title: Long-distance endosome trafficking drives fungal effector production during plant infection
Article Snippet: In brief, leaf samples were removed 2–4 cm beneath the injection point and harvested from four independent experiments, 30 plants each. .. The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously . .. Putative components of EE-associated signalling were identified by reciprocal BLAST search ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Article Title: Vitamin A status regulates glucocorticoid availability in Wistar rats: consequences on cognitive functions and hippocampal neurogenesis?
Article Snippet: Paragraph title: Real-time PCR analyzes of retinoid target gene expression in the hippocampus ... The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech).

Incubation:

Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome
Article Snippet: RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions. .. RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

Article Title: Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice
Article Snippet: The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech, France). .. The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech, France).

Article Title: In vitro antiplasmodial activity of cepharanthine
Article Snippet: Cultures were pooled and divided into 12 samples to constitute four biological replicates for each the three analytic groups: UT8 and UT16 were the controls 8 hr and 16 hr post-merozoite invasion (h pmi), respectively; T16 was the group exposed to a 8-hr period of cepharanthine incubation started at 8 hr pmi (Figure ). .. RNA samples were checked for integrity using the NanoDrop ND-1000 (Labtech, Palaiseau, France) and qualified using a 2100 Bioanalyzer RNA Nano Chip (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol.

Expressing:

Article Title: Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice
Article Snippet: Hippocampal and striatal gene expression were measured as previously described (Touyarot et al., ). .. The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech, France).

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: Paragraph title: mRNA Expression Analysis by Real-time PCR ... The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France).

Article Title: Long-distance endosome trafficking drives fungal effector production during plant infection
Article Snippet: In brief, leaf samples were removed 2–4 cm beneath the injection point and harvested from four independent experiments, 30 plants each. .. The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously . .. Putative components of EE-associated signalling were identified by reciprocal BLAST search ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Article Title: Vitamin A status regulates glucocorticoid availability in Wistar rats: consequences on cognitive functions and hippocampal neurogenesis?
Article Snippet: Paragraph title: Real-time PCR analyzes of retinoid target gene expression in the hippocampus ... The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech).

Article Title: Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
Article Snippet: Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water. .. Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water.

Modification:

Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome
Article Snippet: Specifically, RNA was extracted using TriReagent (Sigma-Aldrich, T9424) using a modified procedure that we had previously reported [ ]. .. RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

Western Blot:

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France). .. The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France).

SYBR Green Assay:

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France). .. The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France).

Article Title: Long-distance endosome trafficking drives fungal effector production during plant infection
Article Snippet: In brief, leaf samples were removed 2–4 cm beneath the injection point and harvested from four independent experiments, 30 plants each. .. The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously . .. Putative components of EE-associated signalling were identified by reciprocal BLAST search ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Article Title: Vitamin A status regulates glucocorticoid availability in Wistar rats: consequences on cognitive functions and hippocampal neurogenesis?
Article Snippet: The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech). .. Using oligodT and random primers (Promega, Charbonnières les bains, France), cDNA was synthesized from 1 μg of RNA with ImPromII reverse transcriptase (Promega, Charbonnières les Bains, France) according to the manufacturer protocol.

Cell Culture:

Article Title: MLVA for Salmonella enterica subsp. enterica Serovar Dublin: Development of a Method Suitable for Inter-Laboratory Surveillance and Application in the Context of a Raw Milk Cheese Outbreak in France in 2012
Article Snippet: Strains were cultured overnight at 37°C on tryptone soya yeast extract agar plate. .. The DNA concentration was measured with Nanodrop ND-1000 (Labtech, France).

Sequencing:

Article Title: Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
Article Snippet: Paragraph title: Sample preparation and mRNA sequencing ... Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water.

Injection:

Article Title: Long-distance endosome trafficking drives fungal effector production during plant infection
Article Snippet: The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously . .. The material was frozen in liquid nitrogen, ground by mortar and pestle, and total RNA was extracted by using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) with DNase I treatment, according to the manufacturer’s instructions. cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, USA). qRT–PCR was performed with an Mx3005P thermocycler (Stratagene, La Jolla, USA) by using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Stratagene) and ~250 or 500 ng cDNAs, estimated by NanoDrop ND-1000 (LabTech International Ltd, East Sussex, UK), from samples of liquid cultures or plants, respectively. qRT–PCR conditions were as follows: 3 min at 95 °C, followed by 40 cycles of 95 °C for 20 s and 60 °C for 20 s. To normalize expression levels, the constitutively expressed peptidyl–prolyl cis–trans isomerase gene (ppi ) was used, as described previously .

DNA Extraction:

Article Title: MLVA for Salmonella enterica subsp. enterica Serovar Dublin: Development of a Method Suitable for Inter-Laboratory Surveillance and Application in the Context of a Raw Milk Cheese Outbreak in France in 2012
Article Snippet: Paragraph title: DNA Extraction ... The DNA concentration was measured with Nanodrop ND-1000 (Labtech, France).

Isolation:

Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome
Article Snippet: RNA was isolated from 250 μL of each of the plasma specimens. .. RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Liver from all 10 individual fish per dietary treatment and population (n = 40) were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, United Kingdom) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, United States). .. Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. To perform the RNA amplifications, 2500 ng RNA from each of two fish from the same tank and population were pooled to obtain five samples per treatment and stock (n = 5).

Article Title: Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: Biochemical, pharmacological and toxicological implications
Article Snippet: For the proteomic study, livers were harvested from wild type and Nrf2(−/−) mice that had been dosed with 3 mg/kg CDDO-me or DMSO vehicle control (n = 6), using the protocol as described for the pilot study. .. 2.3 RNA isolation was performed using the RNeasy Mini-kit according to the manufacturer’s instructions, and RNA concentration was determined using a NanoDrop ND-1000 (Labtech, East Sussex, UK). .. The quality of the RNA was determined using the Agilent RNA 6000 Nano Kit according to the manufacturer’s instructions, with samples analysed using the Agilent 2100 bioanalyzer (Agilent, Berkshire, UK).

Article Title: A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish
Article Snippet: Liver from six individual fish per dietary treatment were homogenised in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, USA). .. Total RNA was isolated following manufacturer's instructions and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. Transcriptome analysis was performed in liver tissues using an Atlantic salmon custom-made oligoarray with 44 k features per array on a four-array-per-slide format (Agilent Technologies UK Ltd., Wokingham, UK).

Article Title: An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
Article Snippet: Individual methyl esters were identified by comparison with known standards and a well-characterized fish oil, and also by reference to published data [ ]. .. Liver, midgut and hindgut from eighteen individual fish per dietary treatment were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK), total RNA isolated following manufacturer’s instructions, and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 200 ng of total RNA in a 1% agarose gel. .. Additionally the Agilent Bioanalyzer with the RNA LabChip kit (Agilent Technologies) was used to analyze approximately 300 ng of total RNA from a randomly selected number of samples (72 samples; 12 samples per treatment and tissue) and provide an RNA integrity number (RIN), which was higher than 8.0 in all samples (average RIN = 8.2). cDNA was synthesized as detailed in [ ] and samples pooled to obtain n = 6 per dietary treatment.

Article Title: Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
Article Snippet: After 5 days (fourth stage larvae), 30 fresh larvae from each batch were used for total RNA extraction using the PicoPure™ RNA isolation kit (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturer's instructions. .. Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water.

Article Title: WNK1 kinase and its partners Akt, SGK1 and NBC-family Na+/HCO3− cotransporters are potential therapeutic targets for glioblastoma stem-like cells linked to Bisacodyl signaling
Article Snippet: Total RNA was isolated from 5–10×106 proliferating or quiescent (9 days without medium renewal) TG1 and TG1-C1 GSC using the TRI Reagent (Euromedex, France) according to the manufacturer’s instructions. .. NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment.

Purification:

Article Title: Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice
Article Snippet: The integrity of the purified RNA was verified using the RNA 6000 Nano LabChip kit in combination with the 2100 Bioanalyzer (Agilent Technologies, France). .. The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech, France).

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: The organic phase containing proteins was stored at -20°C for subsequent Western blot analysis. .. The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France). .. The integrity of RNA samples was assessed using the RNA 6000 Nano LabChip kit in combination with the 2100 Bioanalyzer (Agilent Technologies, France).

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Liver from all 10 individual fish per dietary treatment and population (n = 40) were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, United Kingdom) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, United States). .. Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. To perform the RNA amplifications, 2500 ng RNA from each of two fish from the same tank and population were pooled to obtain five samples per treatment and stock (n = 5).

Article Title: Vitamin A status regulates glucocorticoid availability in Wistar rats: consequences on cognitive functions and hippocampal neurogenesis?
Article Snippet: The integrity of the purified RNA was verified using the RNA 6000 Nano LabChip kit in combination with the 2100 Bioanalyzer (Agilent Technologies). .. The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech).

Article Title: WNK1 kinase and its partners Akt, SGK1 and NBC-family Na+/HCO3− cotransporters are potential therapeutic targets for glioblastoma stem-like cells linked to Bisacodyl signaling
Article Snippet: RNeasy mini kit columns (Qiagen) were used for further purification of RNA samples. .. NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment.

cDNA Library Assay:

Article Title: Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
Article Snippet: Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water. .. Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water.

Agarose Gel Electrophoresis:

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Liver from all 10 individual fish per dietary treatment and population (n = 40) were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, United Kingdom) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, United States). .. Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. To perform the RNA amplifications, 2500 ng RNA from each of two fish from the same tank and population were pooled to obtain five samples per treatment and stock (n = 5).

Article Title: A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish
Article Snippet: Liver from six individual fish per dietary treatment were homogenised in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, USA). .. Total RNA was isolated following manufacturer's instructions and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. Transcriptome analysis was performed in liver tissues using an Atlantic salmon custom-made oligoarray with 44 k features per array on a four-array-per-slide format (Agilent Technologies UK Ltd., Wokingham, UK).

Article Title: An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
Article Snippet: Individual methyl esters were identified by comparison with known standards and a well-characterized fish oil, and also by reference to published data [ ]. .. Liver, midgut and hindgut from eighteen individual fish per dietary treatment were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK), total RNA isolated following manufacturer’s instructions, and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 200 ng of total RNA in a 1% agarose gel. .. Additionally the Agilent Bioanalyzer with the RNA LabChip kit (Agilent Technologies) was used to analyze approximately 300 ng of total RNA from a randomly selected number of samples (72 samples; 12 samples per treatment and tissue) and provide an RNA integrity number (RIN), which was higher than 8.0 in all samples (average RIN = 8.2). cDNA was synthesized as detailed in [ ] and samples pooled to obtain n = 6 per dietary treatment.

Chromatin Immunoprecipitation:

Article Title: In vitro antiplasmodial activity of cepharanthine
Article Snippet: RNA was extracted from erythrocyte pellets blocked by TRIZOL™ reagent according to manufacturer’s recommendations (Invitrogen, Carlsbad, CA, USA), and treated with DNase (DNAfree™, Ambion, Foster City, CA, USA). .. RNA samples were checked for integrity using the NanoDrop ND-1000 (Labtech, Palaiseau, France) and qualified using a 2100 Bioanalyzer RNA Nano Chip (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol. .. No trace of DNA contamination was detected by Bioanalyzer analysis.

Software:

Article Title: WNK1 kinase and its partners Akt, SGK1 and NBC-family Na+/HCO3− cotransporters are potential therapeutic targets for glioblastoma stem-like cells linked to Bisacodyl signaling
Article Snippet: NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment. .. NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment.

Electrophoresis:

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Liver from all 10 individual fish per dietary treatment and population (n = 40) were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, United Kingdom) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, United States). .. Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. To perform the RNA amplifications, 2500 ng RNA from each of two fish from the same tank and population were pooled to obtain five samples per treatment and stock (n = 5).

Article Title: A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish
Article Snippet: Liver from six individual fish per dietary treatment were homogenised in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, USA). .. Total RNA was isolated following manufacturer's instructions and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. Transcriptome analysis was performed in liver tissues using an Atlantic salmon custom-made oligoarray with 44 k features per array on a four-array-per-slide format (Agilent Technologies UK Ltd., Wokingham, UK).

Article Title: An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
Article Snippet: Individual methyl esters were identified by comparison with known standards and a well-characterized fish oil, and also by reference to published data [ ]. .. Liver, midgut and hindgut from eighteen individual fish per dietary treatment were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK), total RNA isolated following manufacturer’s instructions, and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 200 ng of total RNA in a 1% agarose gel. .. Additionally the Agilent Bioanalyzer with the RNA LabChip kit (Agilent Technologies) was used to analyze approximately 300 ng of total RNA from a randomly selected number of samples (72 samples; 12 samples per treatment and tissue) and provide an RNA integrity number (RIN), which was higher than 8.0 in all samples (average RIN = 8.2). cDNA was synthesized as detailed in [ ] and samples pooled to obtain n = 6 per dietary treatment.

RNA Extraction:

Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome
Article Snippet: Paragraph title: RNA extraction and characterisation ... RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

Article Title: Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice
Article Snippet: RNA extraction was conducted using TRIzol reagent kit (Invitrogen, France) according to the manufacturer’s instructions. .. The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech, France).

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Paragraph title: RNA Extraction ... Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel.

Article Title: Associations between fetal size, sex and both proliferation and apoptosis at the porcine feto-maternal interface
Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... The RNA was quantified spectrophotometrically using a Nanodrop ND-1000 (Labtech International Ltd., Heathfield, U.K.), and the quality assessed electrophoretically using a Tapestation 2200 (Agilent Technologies, Edinburgh, U.K.) ( ).

Article Title: Associations between fetal size, sex and placental angiogenesis in the pig
Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... The RNA was quantified, and the quality assessed spectrophotometrically using a Nanodrop ND-1000 (Labtech International Ltd) and electrophoretically using a Tapestation 2200 (Agilent Technologies).

Article Title: Vitamin A status regulates glucocorticoid availability in Wistar rats: consequences on cognitive functions and hippocampal neurogenesis?
Article Snippet: RNA extraction was conducted using TRIzol reagent (Invitrogen, Saint Aubin, France) according to the manufacturer's instructions. .. The concentrations of RNA were determined by using a Nanodrop ND-1000 (Labtech).

Article Title: In vitro antiplasmodial activity of cepharanthine
Article Snippet: Paragraph title: Parasitic treatment and RNA extraction ... RNA samples were checked for integrity using the NanoDrop ND-1000 (Labtech, Palaiseau, France) and qualified using a 2100 Bioanalyzer RNA Nano Chip (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol.

Article Title: A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish
Article Snippet: Paragraph title: RNA extraction ... Total RNA was isolated following manufacturer's instructions and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 500 ng of total RNA in a 1% agarose gel.

Article Title: An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... Liver, midgut and hindgut from eighteen individual fish per dietary treatment were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK), total RNA isolated following manufacturer’s instructions, and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 200 ng of total RNA in a 1% agarose gel.

Article Title: Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
Article Snippet: After 5 days (fourth stage larvae), 30 fresh larvae from each batch were used for total RNA extraction using the PicoPure™ RNA isolation kit (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturer's instructions. .. Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water.

Article Title: Association of foetal size and sex with porcine foeto-maternal interface integrin expression
Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... The RNA was quantified, and the quality was assessed spectrophotometrically using a Nanodrop ND-1000 (Labtech International Ltd.) and electrophoretically using a Tapestation 2200 (Agilent Technologies).

Article Title: WNK1 kinase and its partners Akt, SGK1 and NBC-family Na+/HCO3− cotransporters are potential therapeutic targets for glioblastoma stem-like cells linked to Bisacodyl signaling
Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment.

Sample Prep:

Article Title: Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
Article Snippet: Paragraph title: Sample preparation and mRNA sequencing ... Total RNA quality and quantity were measured using a NanoDrop ND-1000 (LabTech, East Sussex, UK) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before dilution to 750 ng/μL in nuclease-free water.

Spectrophotometry:

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: The organic phase containing proteins was stored at -20°C for subsequent Western blot analysis. .. The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France). .. The integrity of RNA samples was assessed using the RNA 6000 Nano LabChip kit in combination with the 2100 Bioanalyzer (Agilent Technologies, France).

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Liver from all 10 individual fish per dietary treatment and population (n = 40) were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, United Kingdom) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, United States). .. Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. To perform the RNA amplifications, 2500 ng RNA from each of two fish from the same tank and population were pooled to obtain five samples per treatment and stock (n = 5).

Article Title: A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish
Article Snippet: Liver from six individual fish per dietary treatment were homogenised in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, USA). .. Total RNA was isolated following manufacturer's instructions and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 500 ng of total RNA in a 1% agarose gel. .. Transcriptome analysis was performed in liver tissues using an Atlantic salmon custom-made oligoarray with 44 k features per array on a four-array-per-slide format (Agilent Technologies UK Ltd., Wokingham, UK).

Article Title: An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
Article Snippet: Individual methyl esters were identified by comparison with known standards and a well-characterized fish oil, and also by reference to published data [ ]. .. Liver, midgut and hindgut from eighteen individual fish per dietary treatment were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK), total RNA isolated following manufacturer’s instructions, and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 200 ng of total RNA in a 1% agarose gel. .. Additionally the Agilent Bioanalyzer with the RNA LabChip kit (Agilent Technologies) was used to analyze approximately 300 ng of total RNA from a randomly selected number of samples (72 samples; 12 samples per treatment and tissue) and provide an RNA integrity number (RIN), which was higher than 8.0 in all samples (average RIN = 8.2). cDNA was synthesized as detailed in [ ] and samples pooled to obtain n = 6 per dietary treatment.

Concentration Assay:

Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome
Article Snippet: Glycogen (Sigma, G8751) (final concentration 120 μg/mL) was used as a carrier and 500 μL isopropanol (Sigma, I9516) was added to precipitate out the RNA. .. RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

Article Title: MLVA for Salmonella enterica subsp. enterica Serovar Dublin: Development of a Method Suitable for Inter-Laboratory Surveillance and Application in the Context of a Raw Milk Cheese Outbreak in France in 2012
Article Snippet: The extraction was performed with Instagene Matrix (Biorad, France) according to the manufacturer’s instructions for Gram-negative organisms. .. The DNA concentration was measured with Nanodrop ND-1000 (Labtech, France). .. The DNA concentration of the samples was normalized at a final concentration of 50 ng/μL.

Article Title: Isolation of human genomic DNA for genetic analysis from premature neonates: a comparison between newborn dried blood spots, whole blood and umbilical cord tissue
Article Snippet: The key steps of the DNA isolation protocols are summarised in Table . .. DNA concentration was measured at 260 nm against nuclease free water using a NanoDrop ND-1000 (Labtech International Ltd, Ringmer East Sussex, UK). .. The purity of gDNA was determined by measuring the 260-280 nm absorbance ratio (A260 :A280 ; Table ).

Article Title: EPA/DHA and Vitamin A Supplementation Improves Spatial Memory and Alleviates the Age-related Decrease in Hippocampal RXRγ and Kinase Expression in Rats
Article Snippet: The organic phase containing proteins was stored at -20°C for subsequent Western blot analysis. .. The concentration of the purified RNA was measured by spectrophotometry at 260 nm using a Nanodrop ND-1000 (Labtech, France). .. The integrity of RNA samples was assessed using the RNA 6000 Nano LabChip kit in combination with the 2100 Bioanalyzer (Agilent Technologies, France).

Article Title: Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: Biochemical, pharmacological and toxicological implications
Article Snippet: For the proteomic study, livers were harvested from wild type and Nrf2(−/−) mice that had been dosed with 3 mg/kg CDDO-me or DMSO vehicle control (n = 6), using the protocol as described for the pilot study. .. 2.3 RNA isolation was performed using the RNeasy Mini-kit according to the manufacturer’s instructions, and RNA concentration was determined using a NanoDrop ND-1000 (Labtech, East Sussex, UK). .. The quality of the RNA was determined using the Agilent RNA 6000 Nano Kit according to the manufacturer’s instructions, with samples analysed using the Agilent 2100 bioanalyzer (Agilent, Berkshire, UK).

Article Title: In vitro antiplasmodial activity of cepharanthine
Article Snippet: Cepharanthine was used at the concentration corresponding to the IC90 estimated graphically from the curve modeled for IC50 calculation on the 3D7 strain. .. RNA samples were checked for integrity using the NanoDrop ND-1000 (Labtech, Palaiseau, France) and qualified using a 2100 Bioanalyzer RNA Nano Chip (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol.

Article Title: WNK1 kinase and its partners Akt, SGK1 and NBC-family Na+/HCO3− cotransporters are potential therapeutic targets for glioblastoma stem-like cells linked to Bisacodyl signaling
Article Snippet: NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment. .. NanoDrop ND-1000 (Labtech) was used for absorption spectra analysis and RNA purity assessment.

Fluorescence In Situ Hybridization:

Article Title: Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Article Snippet: Liver from all 10 individual fish per dietary treatment and population (n = 40) were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, United Kingdom) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, United States). .. Total RNA was isolated following manufacturer’s instructions, purified using a commercial kit (RNeasy Mini Kit, Qiagen, Manchester, United Kingdom) and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, United Kingdom) and electrophoresis using 500 ng of total RNA in a 1% agarose gel.

Article Title: A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish
Article Snippet: Liver from six individual fish per dietary treatment were homogenised in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK) RNA extraction buffer using a bead tissue disruptor (Bio Spec, Bartlesville, Oklahoma, USA). .. Total RNA was isolated following manufacturer's instructions and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 500 ng of total RNA in a 1% agarose gel.

Article Title: An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
Article Snippet: Individual methyl esters were identified by comparison with known standards and a well-characterized fish oil, and also by reference to published data [ ]. .. Liver, midgut and hindgut from eighteen individual fish per dietary treatment were homogenized in 1 ml of TriReagent® (Sigma-Aldrich, Dorset, UK), total RNA isolated following manufacturer’s instructions, and quantity and quality determined by spectrophotometry using a Nanodrop ND-1000 (Labtech Int., East Sussex, UK) and electrophoresis using 200 ng of total RNA in a 1% agarose gel. .. Additionally the Agilent Bioanalyzer with the RNA LabChip kit (Agilent Technologies) was used to analyze approximately 300 ng of total RNA from a randomly selected number of samples (72 samples; 12 samples per treatment and tissue) and provide an RNA integrity number (RIN), which was higher than 8.0 in all samples (average RIN = 8.2). cDNA was synthesized as detailed in [ ] and samples pooled to obtain n = 6 per dietary treatment.

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    LabTech Inc nd 1000
    Plasma-based RNA quantification and characterisation (A) Concentration of RNA isolated from plasma specimens, as assessed by NanoDrop <t>ND-1000</t> spectrophotometer. (B(i)) Electropherograms show the presence of 5S and 5.8S subunits, tRNAs and small RNA fragments (
    Nd 1000, supplied by LabTech Inc, used in various techniques. Bioz Stars score: 92/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Plasma-based RNA quantification and characterisation (A) Concentration of RNA isolated from plasma specimens, as assessed by NanoDrop ND-1000 spectrophotometer. (B(i)) Electropherograms show the presence of 5S and 5.8S subunits, tRNAs and small RNA fragments (

    Journal: Oncotarget

    Article Title: miR-758-3p: a blood-based biomarker that’s influence on the expression of CERP/ABCA1 may contribute to the progression of obesity to metabolic syndrome

    doi: 10.18632/oncotarget.24314

    Figure Lengend Snippet: Plasma-based RNA quantification and characterisation (A) Concentration of RNA isolated from plasma specimens, as assessed by NanoDrop ND-1000 spectrophotometer. (B(i)) Electropherograms show the presence of 5S and 5.8S subunits, tRNAs and small RNA fragments (

    Article Snippet: RNA quantity was subsequently assessed using a NanoDrop ND-1000 (Labtech International, Uckfield, England) and Agilent Pico 6000 and Small RNA kits (Agilent Technologies, CA, USA) as per manufacturers’ instructions.

    Techniques: Concentration Assay, Isolation, Spectrophotometry