nd 1000 spectrophotometer  (Thermo Fisher)


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    Thermo Fisher nd 1000 spectrophotometer
    Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nd 1000 spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 293 article reviews
    Price from $9.99 to $1999.99
    nd 1000 spectrophotometer - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: A High-Efficiency Superhydrophobic Plasma Separator
    Article Snippet: After 10 min, the plasma was recovered, and the S. mansoni DNA in the plasma was detected by real time quantitative loop-mediated isothermal amplification (LAMP). .. Optical density (OD) of the supernatant was measured at 540 nm using a ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE).

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined. .. [ ] DNA amplification was carried out with GoTaq® Hot Start Polymerase (Promega, Madison, WI, USA) and 0.2 μM of each primer using the GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA).

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: One hundred ng of each total RNA sample was used for the linear T7-based amplification and Cy3-labeled cRNA were prepared using the Agilent Low Input Quick Amp Labeling Kit. .. Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA).

    Quantitative RT-PCR:

    Article Title: Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR analysis ... RNA concentration was determined using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA).

    Electrophoresis:

    Article Title: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
    Article Snippet: The concentration of RNA was identified by the optical density ( OD ) at 260 nm, and the purity of RNA was determined the 260 nm/280 nm ratio with expected values between 1.8 and 2.0 using the ND-1000 spectrophotometer (NanoDrop Technologies, Winooski, VT, USA). .. Total RNA integrity was evaluated by electrophoresis on 2% (w/v) agarose gels according to the 28S to 18S rRNA ratio.

    Microarray:

    Article Title: Splice variant rs72613567 prevents worst histologic outcomes in patients with nonalcoholic fatty liver disease
    Article Snippet: After extraction, RNA quantity was measured using the ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE), the ratio of absorbance 260 nm/280 nm being 1.9–2.0. .. The same proportion of NASH and NAFL patients were included per rs72613567-genotype group; selection of cases was based on the availability of RNA of sufficient quality and quantity to perform the microarray experiment. ) and 30,606 long noncoding RNAs.

    Article Title: Formation of phenotypic lineages in Salmonella enterica by a pleiotropic fimbrial switch
    Article Snippet: Paragraph title: RNA isolation, microarray procedures, and data analysis ... The quantity and quality of the RNA was determined using a ND-1000 spectrophotometer (NanoDrop Technologies).

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: Paragraph title: Whole genome microarray hybridization ... Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA).

    Random Hexamer Labeling:

    Article Title: Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes
    Article Snippet: The extracted RNA concentration and purity was estimated using a ND-1000 spectrophotometer (NanoDrop Technologies, Delaware USA). .. Reverse transcription was carried out with 50ng of total RNA using SuperScript®III First-Strand Synthesis System for RT-PCR (Invitrogen™ ) and a random hexamer primer in a final reaction volume of 10 μl according to the manufacturer’s instructions.

    Article Title: Estradiol Prevents High Glucose-Induced β-cell Apoptosis by Decreased BTG2 Expression
    Article Snippet: The concentration of total RNA was measured with a ND-1000 Spectrophotometer (Nanodrop, USA). .. First-strand complementary DNA (cDNA) was generated from 0.5–1 μg of total RNA using the SuperScript III Reverse Transcriptase (RT) and Random Hexamer Primer (Invitrogen, USA) according to the manufacturer’s instructions.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Differential expression of miRNA199b-5p as a novel biomarker for sporadic and hereditary parathyroid tumors
    Article Snippet: Total RNA was extracted from the formalin-fixed and paraffin-embedded (FFPE) samples using TRIzol reagent (GIBCO, BRL, Gaithersburg, MD, USA) according to the manufacturer’s protocol. .. Following extraction, total RNA was quantified by an ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA).

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Genomic deoxyribonucleic acid (DNA) was extracted with QIAamp DNA FFPE Tissue Kit Qiagen (Hilden, Germany) in accordance with the manufacturer's protocol, and cancer cell rich regions were ascertained beforehand using the application of hematoxylin and eosin (H and E) staining to ensure that all cases tested for enrichment of ≥70% tumor cells. .. Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined.

    Expressing:

    Article Title: Splice variant rs72613567 prevents worst histologic outcomes in patients with nonalcoholic fatty liver disease
    Article Snippet: Paragraph title: Gene expression profiling of the liver transcriptome ... After extraction, RNA quantity was measured using the ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE), the ratio of absorbance 260 nm/280 nm being 1.9–2.0.

    Article Title: Formation of phenotypic lineages in Salmonella enterica by a pleiotropic fimbrial switch
    Article Snippet: The quantity and quality of the RNA was determined using a ND-1000 spectrophotometer (NanoDrop Technologies). .. Differential gene expression was assessed using the Limma’s R package [ ].

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Paragraph title: Determination of COX-2/C-MET/KRAS expression status ... Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined.

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA). .. The hybridization of Cy3-labeled cRNA was performed using the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit.

    Hybridization:

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: Paragraph title: Whole genome microarray hybridization ... Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA).

    Lab-on-a-Chip:

    Article Title: RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed
    Article Snippet: RNA concentration was determined using a ND-1000 Spectrophotometer (Thermo Scientific, Illkirch, France). .. Integrity of RNA was checked with Lab-on-a-Chip Eukaryote Total RNA Nano and Bionalyzer 2100 (Agilent Technologies France, Massy, France).

    Generated:

    Article Title: Estradiol Prevents High Glucose-Induced β-cell Apoptosis by Decreased BTG2 Expression
    Article Snippet: The concentration of total RNA was measured with a ND-1000 Spectrophotometer (Nanodrop, USA). .. First-strand complementary DNA (cDNA) was generated from 0.5–1 μg of total RNA using the SuperScript III Reverse Transcriptase (RT) and Random Hexamer Primer (Invitrogen, USA) according to the manufacturer’s instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes
    Article Snippet: The extracted RNA concentration and purity was estimated using a ND-1000 spectrophotometer (NanoDrop Technologies, Delaware USA). .. Reverse transcription was carried out with 50ng of total RNA using SuperScript®III First-Strand Synthesis System for RT-PCR (Invitrogen™ ) and a random hexamer primer in a final reaction volume of 10 μl according to the manufacturer’s instructions.

    Article Title: Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor
    Article Snippet: RNA concentration was determined using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. We performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis by using a LightCycler 480 system (Roche, Mannheim, Germany).

    Software:

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined. .. Primer pairs for gene amplifications were designed with AmpliSeq Designer software, version 1.2.6 software (Life Technologies).

    Staining:

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: The isolated CD45+ cells were collected in staining buffer and kept chilled on ice until RNA extraction could be commenced. .. Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Genomic deoxyribonucleic acid (DNA) was extracted with QIAamp DNA FFPE Tissue Kit Qiagen (Hilden, Germany) in accordance with the manufacturer's protocol, and cancer cell rich regions were ascertained beforehand using the application of hematoxylin and eosin (H and E) staining to ensure that all cases tested for enrichment of ≥70% tumor cells. .. Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined.

    RNA Sequencing Assay:

    Article Title: RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed
    Article Snippet: RNA concentration was determined using a ND-1000 Spectrophotometer (Thermo Scientific, Illkirch, France). .. RNA-seq paired-end libraries have been prepared according to Illumina’s protocols using the Illumina TruSeq RNA Sample Prep Kit v2 to analyze mRNA (Illumina, San Diego, CA).

    Fluorescence:

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: Paragraph title: Fluorescence-Activated Cell Sorting of CD45+ Leukocytes ... Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA). .. Subsequently, the microarrays were washed once with the Agilent Gene Expression Wash Buffer-1 for 1 min at 25 °C followed by a second wash with preheated Agilent Gene Expression Wash Buffer-2 (37 °C) for 1 min Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System.

    Isolation:

    Article Title: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
    Article Snippet: Paragraph title: RNA isolation and reverse transcription ... The concentration of RNA was identified by the optical density ( OD ) at 260 nm, and the purity of RNA was determined the 260 nm/280 nm ratio with expected values between 1.8 and 2.0 using the ND-1000 spectrophotometer (NanoDrop Technologies, Winooski, VT, USA).

    Article Title: Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes
    Article Snippet: Paragraph title: RNA isolation and reverse transcription ... The extracted RNA concentration and purity was estimated using a ND-1000 spectrophotometer (NanoDrop Technologies, Delaware USA).

    Article Title: The mitochondrial genome encodes abundant small noncoding RNAs
    Article Snippet: Paragraph title: Small RNA isolation ... The concentration of small RNAs was measured using a ND-1000 spectrophotometer (NanoDrop Technologies).

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: .. Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. RNA samples were prepared for RNA-seq by the Australian Cancer Research Foundation (ACRF) Biomolecular Resource Facility (John Curtin School of Medical Research, Australian National University, CAN, Australia). cDNA library preparation was performed using the SMARTer Low RNA Kit (Clontech, Mountain View, CA, USA) as per the manufacturer’s instructions, using 679 pg of RNA per sample was used as starting material for the preparation of the cDNA libraries.

    Article Title: Differential expression of miRNA199b-5p as a novel biomarker for sporadic and hereditary parathyroid tumors
    Article Snippet: Paragraph title: RNA isolation ... Following extraction, total RNA was quantified by an ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA).

    Article Title: Formation of phenotypic lineages in Salmonella enterica by a pleiotropic fimbrial switch
    Article Snippet: Paragraph title: RNA isolation, microarray procedures, and data analysis ... The quantity and quality of the RNA was determined using a ND-1000 spectrophotometer (NanoDrop Technologies).

    Article Title: Estradiol Prevents High Glucose-Induced β-cell Apoptosis by Decreased BTG2 Expression
    Article Snippet: Paragraph title: RNA isolation and reverse transcriptase-polymerase chain reaction ... The concentration of total RNA was measured with a ND-1000 Spectrophotometer (Nanodrop, USA).

    Article Title: Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor
    Article Snippet: Paragraph title: RNA isolation and qRT-PCR analysis ... RNA concentration was determined using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA).

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: Whole genome microarray hybridization RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. .. Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA).

    Sequencing:

    Article Title: RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed
    Article Snippet: Paragraph title: RNA preparation and sequencing ... RNA concentration was determined using a ND-1000 Spectrophotometer (Thermo Scientific, Illkirch, France).

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: KRAS mutations were analyzed for each FFPE specimen using polymerase chain reaction (PCR)-based direct gene sequencing. .. Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined.

    Labeling:

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: Viability of the sorted cells was assessed by labeling with DAPI. .. Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: One hundred ng of each total RNA sample was used for the linear T7-based amplification and Cy3-labeled cRNA were prepared using the Agilent Low Input Quick Amp Labeling Kit. .. Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA).

    Purification:

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined. .. [ ] After identification by agarose gel electrophoresis, the PCR products were purified with the DNA Clean/Extraction Kit (GeneMark), then submitted to direct sequencing on an ABI 3730xl genetic analyzer (Invitrogen Life Technologies, Carlsbad, CA, USA), and detailed steps were consistent with those reported earlier.

    Polymerase Chain Reaction:

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: KRAS mutations were analyzed for each FFPE specimen using polymerase chain reaction (PCR)-based direct gene sequencing. .. Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined.

    Lysis:

    Article Title: Formation of phenotypic lineages in Salmonella enterica by a pleiotropic fimbrial switch
    Article Snippet: After lysis, RNA was extracted using 1 ml of TRIsure reagent following manufacture’s instructions (Bioline, Taunton, Massachusetts, USA). .. The quantity and quality of the RNA was determined using a ND-1000 spectrophotometer (NanoDrop Technologies).

    Sample Prep:

    Article Title: RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed
    Article Snippet: RNA concentration was determined using a ND-1000 Spectrophotometer (Thermo Scientific, Illkirch, France). .. RNA-seq paired-end libraries have been prepared according to Illumina’s protocols using the Illumina TruSeq RNA Sample Prep Kit v2 to analyze mRNA (Illumina, San Diego, CA).

    TaqMan Assay:

    Article Title: Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation
    Article Snippet: RNA and DNA concentrations were measured with an ND-1000 Spectrophotometer (NanoDrop, Wilmington, DE). .. HIV RNA copy numbers were measured with a locked nucleic acid (LNA) Taqman assay.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
    Article Snippet: RNA quality was analyzed basing on strict processes as indicated in the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines [ ]. .. The concentration of RNA was identified by the optical density ( OD ) at 260 nm, and the purity of RNA was determined the 260 nm/280 nm ratio with expected values between 1.8 and 2.0 using the ND-1000 spectrophotometer (NanoDrop Technologies, Winooski, VT, USA).

    Article Title: Estradiol Prevents High Glucose-Induced β-cell Apoptosis by Decreased BTG2 Expression
    Article Snippet: The concentration of total RNA was measured with a ND-1000 Spectrophotometer (Nanodrop, USA). .. The rat primers for real-time PCR were as follows.

    RNA Extraction:

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: RNA extraction was performed with a combination of TRIzol Reagent (Life Technologies, Carlsbad, CA, USA) and an RNAqueous-small scale kit (Life Technologies, Carlsbad, CA, USA) utilized in tandem to extract and purify the RNA, respectively. .. Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).

    Selection:

    Article Title: Splice variant rs72613567 prevents worst histologic outcomes in patients with nonalcoholic fatty liver disease
    Article Snippet: After extraction, RNA quantity was measured using the ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE), the ratio of absorbance 260 nm/280 nm being 1.9–2.0. .. The same proportion of NASH and NAFL patients were included per rs72613567-genotype group; selection of cases was based on the availability of RNA of sufficient quality and quantity to perform the microarray experiment. ) and 30,606 long noncoding RNAs.

    Agarose Gel Electrophoresis:

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined. .. [ ] After identification by agarose gel electrophoresis, the PCR products were purified with the DNA Clean/Extraction Kit (GeneMark), then submitted to direct sequencing on an ABI 3730xl genetic analyzer (Invitrogen Life Technologies, Carlsbad, CA, USA), and detailed steps were consistent with those reported earlier.

    Article Title: Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor
    Article Snippet: RNA concentration was determined using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. RNA integrity was confirmed through 1% agarose gel electrophoresis.

    Incubation:

    Article Title: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
    Article Snippet: The concentration of RNA was identified by the optical density ( OD ) at 260 nm, and the purity of RNA was determined the 260 nm/280 nm ratio with expected values between 1.8 and 2.0 using the ND-1000 spectrophotometer (NanoDrop Technologies, Winooski, VT, USA). .. To remove contaminating DNA, the first reaction mixture consisted of 2 μL 5× gDNA Buffer and 1000 ng RNA template in a total volume of 10 μL, and followed by incubation at 42°C for 3 min.

    Spectrophotometry:

    Article Title: Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation
    Article Snippet: .. RNA and DNA concentrations were measured with an ND-1000 Spectrophotometer (NanoDrop, Wilmington, DE). .. Supernatant HIV-1 RNA was extracted with the QIAamp Viral RNA mini kit (Qiagen, Valencia, CA).

    Article Title: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
    Article Snippet: .. The concentration of RNA was identified by the optical density ( OD ) at 260 nm, and the purity of RNA was determined the 260 nm/280 nm ratio with expected values between 1.8 and 2.0 using the ND-1000 spectrophotometer (NanoDrop Technologies, Winooski, VT, USA). .. Total RNA integrity was evaluated by electrophoresis on 2% (w/v) agarose gels according to the 28S to 18S rRNA ratio.

    Article Title: Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes
    Article Snippet: .. The extracted RNA concentration and purity was estimated using a ND-1000 spectrophotometer (NanoDrop Technologies, Delaware USA). ..

    Article Title: The mitochondrial genome encodes abundant small noncoding RNAs
    Article Snippet: .. The concentration of small RNAs was measured using a ND-1000 spectrophotometer (NanoDrop Technologies). .. For 454 sequencing, small RNA cDNA (srcDNA) libraries were prepared as described .

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: .. Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. RNA samples were prepared for RNA-seq by the Australian Cancer Research Foundation (ACRF) Biomolecular Resource Facility (John Curtin School of Medical Research, Australian National University, CAN, Australia). cDNA library preparation was performed using the SMARTer Low RNA Kit (Clontech, Mountain View, CA, USA) as per the manufacturer’s instructions, using 679 pg of RNA per sample was used as starting material for the preparation of the cDNA libraries.

    Article Title: Differential expression of miRNA199b-5p as a novel biomarker for sporadic and hereditary parathyroid tumors
    Article Snippet: .. Following extraction, total RNA was quantified by an ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA). .. For the miRNA microarray study, 3 sporadic and 3 MEN1-related parathyroid tumors and 2 normal parathyroid tissue samples were used.

    Article Title: Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies
    Article Snippet: .. DNA samples were then quantified using a ND-1000 Spectrophotometer (Thermo Fisher Scientific). .. Total protein was extracted from native or decellularized RPE/choroid punches using the Complete Protease Inhibitor kit (Roche Diagnostic, Indianapolis, IN) with PBS and 1% Triton X-100.

    Article Title: Splice variant rs72613567 prevents worst histologic outcomes in patients with nonalcoholic fatty liver disease
    Article Snippet: .. After extraction, RNA quantity was measured using the ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE), the ratio of absorbance 260 nm/280 nm being 1.9–2.0. ..

    Article Title: A High-Efficiency Superhydrophobic Plasma Separator
    Article Snippet: .. Optical density (OD) of the supernatant was measured at 540 nm using a ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). .. De-identified, EDTA anticoagulated, whole blood samples from healthy donors were collected by the Hospital of the University of Pennsylvania with the approval of the Institutional Review Board (protocol: 814752).

    Article Title: Formation of phenotypic lineages in Salmonella enterica by a pleiotropic fimbrial switch
    Article Snippet: .. The quantity and quality of the RNA was determined using a ND-1000 spectrophotometer (NanoDrop Technologies). ..

    Article Title: Estradiol Prevents High Glucose-Induced β-cell Apoptosis by Decreased BTG2 Expression
    Article Snippet: .. The concentration of total RNA was measured with a ND-1000 Spectrophotometer (Nanodrop, USA). .. First-strand complementary DNA (cDNA) was generated from 0.5–1 μg of total RNA using the SuperScript III Reverse Transcriptase (RT) and Random Hexamer Primer (Invitrogen, USA) according to the manufacturer’s instructions.

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: .. Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined. .. Primer pairs for gene amplifications were designed with AmpliSeq Designer software, version 1.2.6 software (Life Technologies).

    Article Title: Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor
    Article Snippet: .. RNA concentration was determined using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. RNA integrity was confirmed through 1% agarose gel electrophoresis.

    Article Title: T4SS-dependent TLR5 activation by Helicobacter pylori infection
    Article Snippet: .. Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, USA). .. The hybridization of Cy3-labeled cRNA was performed using the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit.

    Produced:

    Article Title: A High-Efficiency Superhydrophobic Plasma Separator
    Article Snippet: Positive and negative controls were, respectively, produced by adding 200 μL of whole blood to 4 mL of distilled water and to isotonic saline. .. Optical density (OD) of the supernatant was measured at 540 nm using a ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE).

    Concentration Assay:

    Article Title: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
    Article Snippet: .. The concentration of RNA was identified by the optical density ( OD ) at 260 nm, and the purity of RNA was determined the 260 nm/280 nm ratio with expected values between 1.8 and 2.0 using the ND-1000 spectrophotometer (NanoDrop Technologies, Winooski, VT, USA). .. Total RNA integrity was evaluated by electrophoresis on 2% (w/v) agarose gels according to the 28S to 18S rRNA ratio.

    Article Title: Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes
    Article Snippet: .. The extracted RNA concentration and purity was estimated using a ND-1000 spectrophotometer (NanoDrop Technologies, Delaware USA). ..

    Article Title: The mitochondrial genome encodes abundant small noncoding RNAs
    Article Snippet: .. The concentration of small RNAs was measured using a ND-1000 spectrophotometer (NanoDrop Technologies). .. For 454 sequencing, small RNA cDNA (srcDNA) libraries were prepared as described .

    Article Title: A High-Efficiency Superhydrophobic Plasma Separator
    Article Snippet: To determine whether the superhydrophobic surfaces can reduce biomolecular adhesion, we sandwiched 100 μL of plasma sample spiked with a known S. mansoni DNA concentration (50 fg) between the two spray-on superhydrophobic substrates. .. Optical density (OD) of the supernatant was measured at 540 nm using a ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE).

    Article Title: Estradiol Prevents High Glucose-Induced β-cell Apoptosis by Decreased BTG2 Expression
    Article Snippet: .. The concentration of total RNA was measured with a ND-1000 Spectrophotometer (Nanodrop, USA). .. First-strand complementary DNA (cDNA) was generated from 0.5–1 μg of total RNA using the SuperScript III Reverse Transcriptase (RT) and Random Hexamer Primer (Invitrogen, USA) according to the manufacturer’s instructions.

    Article Title: COX-2/C-MET/KRAS status-based prognostic nomogram for colorectal cancer: A multicenter cohort study
    Article Snippet: .. Subsequently, DNA concentration was measured using an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and KRAS mutations (including exons 2, 3, and 4) of all tumor samples were determined. .. Primer pairs for gene amplifications were designed with AmpliSeq Designer software, version 1.2.6 software (Life Technologies).

    Article Title: Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor
    Article Snippet: .. RNA concentration was determined using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. RNA integrity was confirmed through 1% agarose gel electrophoresis.

    FACS:

    Article Title: Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome
    Article Snippet: Paragraph title: Fluorescence-Activated Cell Sorting of CD45+ Leukocytes ... Isolated total RNA was analyzed for quantity and purity with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).

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    Thermo Fisher nano drop nd 2000
    Nano Drop Nd 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop nd 2000/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nano drop nd 2000 - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher nano drop nd2000c spectrophotometer
    Nano Drop Nd2000c Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop nd2000c spectrophotometer/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nano drop nd2000c spectrophotometer - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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