nd 1000 spectrophotometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nd 1000 spectrophotometer
    Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nd 1000 spectrophotometer/product/Thermo Fisher
    Average 93 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    nd 1000 spectrophotometer - by Bioz Stars, 2020-09
    93/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat
    Article Snippet: .. Final elution was performed with 40 µl DNA/RNAase-Free water supplied and the eluted RNA was subsequently quantified using a ND-1000 Spectrophotometer (NanoDrop Technologies) and quantified on a 1% (w/v) agarose gel (Bioline) by electrophoresis. cDNA synthesis was performed on 500 ng of RNA using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to the manufacturer’s instruction in a 20 µl reaction and stored at −20 °C until use. .. TaHKT1;5-D coding sequence amplification and sequencing The entire coding sequence of the TaHKT1;5-D gene from Mocho de Espiga Branca, Gladius and Scout were amplified using Phusion® High-Fidelity DNA polymerase (New England Biolabs) following the manufacturer’s instruction.

    Agarose Gel Electrophoresis:

    Article Title: A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat
    Article Snippet: .. Final elution was performed with 40 µl DNA/RNAase-Free water supplied and the eluted RNA was subsequently quantified using a ND-1000 Spectrophotometer (NanoDrop Technologies) and quantified on a 1% (w/v) agarose gel (Bioline) by electrophoresis. cDNA synthesis was performed on 500 ng of RNA using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to the manufacturer’s instruction in a 20 µl reaction and stored at −20 °C until use. .. TaHKT1;5-D coding sequence amplification and sequencing The entire coding sequence of the TaHKT1;5-D gene from Mocho de Espiga Branca, Gladius and Scout were amplified using Phusion® High-Fidelity DNA polymerase (New England Biolabs) following the manufacturer’s instruction.

    Quantitative RT-PCR:

    Article Title: A Single Bolus of Docosahexaenoic Acid Promotes Neuroplastic Changes in the Innervation of Spinal Cord Interneurons and Motor Neurons and Improves Functional Recovery after Spinal Cord Injury
    Article Snippet: .. Total RNA concentration was quantified using an ND 1000 spectrophotometer (NanoDrop Technologies). qRT-PCR analysis of miR-21 expression was performed using TaqMan miRNA assays for miR-21 (assay ID: 000397) and RNU6B as internal control (assay ID: 001093) according to the manufacturer's instructions and performed on 7500 Real-Time PCR System (Applied Biosystems). .. These results show that a single bolus injection of DHA induced significant functional improvement after cervical SCI, and this is similar to the functional improvement previously reported after a thoracic cord hemisection ( ).

    Purification:

    Article Title: Microbiome–host systems interactions: Protective effects of propionate upon the blood–brain barrier
    Article Snippet: .. After purification, the cDNA was quantified using the ND-1000 Spectrophotometer (NanoDrop, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: A Single Bolus of Docosahexaenoic Acid Promotes Neuroplastic Changes in the Innervation of Spinal Cord Interneurons and Motor Neurons and Improves Functional Recovery after Spinal Cord Injury
    Article Snippet: .. Total RNA concentration was quantified using an ND 1000 spectrophotometer (NanoDrop Technologies). qRT-PCR analysis of miR-21 expression was performed using TaqMan miRNA assays for miR-21 (assay ID: 000397) and RNU6B as internal control (assay ID: 001093) according to the manufacturer's instructions and performed on 7500 Real-Time PCR System (Applied Biosystems). .. These results show that a single bolus injection of DHA induced significant functional improvement after cervical SCI, and this is similar to the functional improvement previously reported after a thoracic cord hemisection ( ).

    Concentration Assay:

    Article Title: Data Aggregation at the Level of Molecular Pathways Improves Stability of Experimental Transcriptomic and Proteomic Data
    Article Snippet: .. The cRNA concentration was quantified and adjusted to 150 ng/ml using an ND-1000 Spectrophotometer (NanoDrop Technologies, USA). ..

    Article Title: A Single Bolus of Docosahexaenoic Acid Promotes Neuroplastic Changes in the Innervation of Spinal Cord Interneurons and Motor Neurons and Improves Functional Recovery after Spinal Cord Injury
    Article Snippet: .. Total RNA concentration was quantified using an ND 1000 spectrophotometer (NanoDrop Technologies). qRT-PCR analysis of miR-21 expression was performed using TaqMan miRNA assays for miR-21 (assay ID: 000397) and RNU6B as internal control (assay ID: 001093) according to the manufacturer's instructions and performed on 7500 Real-Time PCR System (Applied Biosystems). .. These results show that a single bolus injection of DHA induced significant functional improvement after cervical SCI, and this is similar to the functional improvement previously reported after a thoracic cord hemisection ( ).

    Article Title: Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells
    Article Snippet: .. The RNA concentration was determined by ND-1000 spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). .. The electrophoretic analysis of 28S and 18S rRNA subunits was used to assess the RNA integrity.

    Spectrophotometry:

    Article Title: Data Aggregation at the Level of Molecular Pathways Improves Stability of Experimental Transcriptomic and Proteomic Data
    Article Snippet: .. The cRNA concentration was quantified and adjusted to 150 ng/ml using an ND-1000 Spectrophotometer (NanoDrop Technologies, USA). ..

    Article Title: Heterobasidion annosum Induces Apoptosis in DLD-1 Cells and Decreases Colon Cancer Growth in In Vivo Model
    Article Snippet: .. The purity and quantity of the DNA samples were evaluated using an ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). .. PCRs were performed using DreamTaq Green PCR Master Mix (Thermo Scientific, Waltham, MA, USA) in a T-Personal thermal cycler (Biometra, Göttingen, Germany).

    Article Title: A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat
    Article Snippet: .. Final elution was performed with 40 µl DNA/RNAase-Free water supplied and the eluted RNA was subsequently quantified using a ND-1000 Spectrophotometer (NanoDrop Technologies) and quantified on a 1% (w/v) agarose gel (Bioline) by electrophoresis. cDNA synthesis was performed on 500 ng of RNA using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to the manufacturer’s instruction in a 20 µl reaction and stored at −20 °C until use. .. TaHKT1;5-D coding sequence amplification and sequencing The entire coding sequence of the TaHKT1;5-D gene from Mocho de Espiga Branca, Gladius and Scout were amplified using Phusion® High-Fidelity DNA polymerase (New England Biolabs) following the manufacturer’s instruction.

    Article Title: A Single Bolus of Docosahexaenoic Acid Promotes Neuroplastic Changes in the Innervation of Spinal Cord Interneurons and Motor Neurons and Improves Functional Recovery after Spinal Cord Injury
    Article Snippet: .. Total RNA concentration was quantified using an ND 1000 spectrophotometer (NanoDrop Technologies). qRT-PCR analysis of miR-21 expression was performed using TaqMan miRNA assays for miR-21 (assay ID: 000397) and RNU6B as internal control (assay ID: 001093) according to the manufacturer's instructions and performed on 7500 Real-Time PCR System (Applied Biosystems). .. These results show that a single bolus injection of DHA induced significant functional improvement after cervical SCI, and this is similar to the functional improvement previously reported after a thoracic cord hemisection ( ).

    Article Title: Phenotype and molecular signature of CD8+ T cell subsets in T cell- mediated rejections after kidney transplantation
    Article Snippet: .. RNA purity and integrity were evaluated by ND-1000 Spectrophotometer (NanoDrop, Wilmington, DE, USA). .. Affymetrix whole transcript expression array methodWe carried out the Affymetrix Whole Transcript Expression array process according to the manufacturer's protocol (GeneChip WT Pico Reagent Kit).

    Article Title: Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells
    Article Snippet: .. The RNA concentration was determined by ND-1000 spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). .. The electrophoretic analysis of 28S and 18S rRNA subunits was used to assess the RNA integrity.

    Article Title: Microbiome–host systems interactions: Protective effects of propionate upon the blood–brain barrier
    Article Snippet: .. After purification, the cDNA was quantified using the ND-1000 Spectrophotometer (NanoDrop, USA). ..

    Article Title: IL-17A both initiates (via IFNγ suppression) and limits the pulmonary type-2 immune response to nematode infection
    Article Snippet: .. RNA was quantified using a ND-1000 Spectrophotometer (NanoDrop Technologies). .. Reverse transcription of 1 μg of total RNA was performed using Tetro reverse transcriptase (Bioline).

    Expressing:

    Article Title: A Single Bolus of Docosahexaenoic Acid Promotes Neuroplastic Changes in the Innervation of Spinal Cord Interneurons and Motor Neurons and Improves Functional Recovery after Spinal Cord Injury
    Article Snippet: .. Total RNA concentration was quantified using an ND 1000 spectrophotometer (NanoDrop Technologies). qRT-PCR analysis of miR-21 expression was performed using TaqMan miRNA assays for miR-21 (assay ID: 000397) and RNU6B as internal control (assay ID: 001093) according to the manufacturer's instructions and performed on 7500 Real-Time PCR System (Applied Biosystems). .. These results show that a single bolus injection of DHA induced significant functional improvement after cervical SCI, and this is similar to the functional improvement previously reported after a thoracic cord hemisection ( ).

    Control Assay:

    Article Title: A Single Bolus of Docosahexaenoic Acid Promotes Neuroplastic Changes in the Innervation of Spinal Cord Interneurons and Motor Neurons and Improves Functional Recovery after Spinal Cord Injury
    Article Snippet: .. Total RNA concentration was quantified using an ND 1000 spectrophotometer (NanoDrop Technologies). qRT-PCR analysis of miR-21 expression was performed using TaqMan miRNA assays for miR-21 (assay ID: 000397) and RNU6B as internal control (assay ID: 001093) according to the manufacturer's instructions and performed on 7500 Real-Time PCR System (Applied Biosystems). .. These results show that a single bolus injection of DHA induced significant functional improvement after cervical SCI, and this is similar to the functional improvement previously reported after a thoracic cord hemisection ( ).

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    Thermo Fisher nano drop 1000 spectrophotometer
    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by <t>Nano-drop</t> 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P
    Nano Drop 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop 1000 spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 13132 article reviews
    Price from $9.99 to $1999.99
    nano drop 1000 spectrophotometer - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher nanodrop spectrophotometer nd 1000
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nanodrop Spectrophotometer Nd 1000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop spectrophotometer nd 1000/product/Thermo Fisher
    Average 94 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    nanodrop spectrophotometer nd 1000 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    85
    Thermo Fisher nano drop kit
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nano Drop Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop kit/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano drop kit - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Journal: MethodsX

    Article Title: Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction

    doi: 10.1016/j.mex.2019.10.015

    Figure Lengend Snippet: RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Article Snippet: The yield of total RNA was determined by Nano-drop 1000 Spectrophotometer (Thermo Scientific).

    Techniques: Lysis, Spectrophotometry, RNA Extraction

    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Journal: Veterinary Research

    Article Title: Visualizing bovine leukemia virus (BLV)-infected cells and measuring BLV proviral loads in the milk of BLV seropositive dams

    doi: 10.1186/s13567-019-0724-1

    Figure Lengend Snippet: Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Article Snippet: The quantity and quality of DNA samples extracted from milk sample was determined based on the A260/280 ratio using a Nanodrop Spectrophotometer ND-1000 (Thermo Fisher Scientific).

    Techniques: Infection, Spectrophotometry