ncii  (New England Biolabs)


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    Structured Review

    New England Biolabs ncii
    Types of restriction profiles of blaA with <t>NciI</t> (Lanes 1–2) and HaeIII (Lanes 3–5), and blaB with HaeIII (Lanes 6–7) and RsaI (Lanes 8–9). <t>PCR</t> amplification and restriction analysis were carried out for 49 strains of Y. intermedia and Y. frederiksenii . M, molecular weight DNA marker (100 bp DNA ladder)
    Ncii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncii/product/New England Biolabs
    Average 86 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    ncii - by Bioz Stars, 2022-08
    86/100 stars

    Images

    1) Product Images from "Characteristics of ?-lactamases and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii"

    Article Title: Characteristics of ?-lactamases and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-7-25

    Types of restriction profiles of blaA with NciI (Lanes 1–2) and HaeIII (Lanes 3–5), and blaB with HaeIII (Lanes 6–7) and RsaI (Lanes 8–9). PCR amplification and restriction analysis were carried out for 49 strains of Y. intermedia and Y. frederiksenii . M, molecular weight DNA marker (100 bp DNA ladder)
    Figure Legend Snippet: Types of restriction profiles of blaA with NciI (Lanes 1–2) and HaeIII (Lanes 3–5), and blaB with HaeIII (Lanes 6–7) and RsaI (Lanes 8–9). PCR amplification and restriction analysis were carried out for 49 strains of Y. intermedia and Y. frederiksenii . M, molecular weight DNA marker (100 bp DNA ladder)

    Techniques Used: Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    2) Product Images from "CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes"

    Article Title: CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes

    Journal: Blood

    doi: 10.1182/blood-2015-10-675751

    Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red
    Figure Legend Snippet: Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red

    Techniques Used: Isolation, Transfection, Polymerase Chain Reaction, Amplification

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    New England Biolabs nci i
    Gene targeting of zebrafish rh1-1 . A : Schematic representation of the zebrafish rh1-1 locus characterized by a single exon encoding 354 amino acid proteins and 5′ and 3′ UTRs. B : Alignment of Rho N- and C-terminal amino acid sequences across species. Numbering is based on the predicted zebrafish rh1–1 . The conserved N-linked glycosylation sequence and VXPX targeting sequence are underlined. C : DNA sequence and amino acid overlay of zebrafish rh1–1 5′- (left) and 3′- (right) coding sequences. Blue text represents the CRISPR PAM sequence. Underlined DNA represents the target for guide RNA hybridization. The purple overscore represent restriction sites for <t>Nci</t> I or Bbs I. Arrows indicate predicted Cas9 cleavage sites. D : Diagrammatic representation of the 253-bp 5′- rh1–1 gene amplicon and the predicted products following digestion with Nci I. E : RFLP analysis of a 253-bp PCR product spanning the 5′ rh1–1 gene (C, undigested control) and digested with Nci I (N) shows retention of the original amplicon (arrow) from pooled DNA from injected embryos versus complete digestion of the PCR product of uninjected control DNA (arrowhead). F : Diagrammatic representation of the 325-bp 3′-amplicon and the predicted products following digestion with Bbs I. G : RFLP analysis of a 325-bp PCR product (C, undigested control) spanning the 3′ rh1–1 sequence digested with Bbs I (B). The PCR product (arrow) from uninjected-controls was digested to near completion, yielding two visible bands of 102 and 182 bp. The 284-bp band (arrowhead) following loss of the 5′- Bbs I site is clearly visible in the injected embryos relative to the controls. The following sequences were used for the RHO alignment: Danio rerio ( NP571159.1 ), X. laevis ( NP001080517.1 ), Mus musculus ( NP663358.1 ), Bos taurus ( NP001014890.1 ), and Homo sapiens ( NP000530.1 ).
    Nci I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nci i/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nci i - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    86
    New England Biolabs ncii
    Types of restriction profiles of blaA with <t>NciI</t> (Lanes 1–2) and HaeIII (Lanes 3–5), and blaB with HaeIII (Lanes 6–7) and RsaI (Lanes 8–9). <t>PCR</t> amplification and restriction analysis were carried out for 49 strains of Y. intermedia and Y. frederiksenii . M, molecular weight DNA marker (100 bp DNA ladder)
    Ncii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncii/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncii - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    Image Search Results


    Gene targeting of zebrafish rh1-1 . A : Schematic representation of the zebrafish rh1-1 locus characterized by a single exon encoding 354 amino acid proteins and 5′ and 3′ UTRs. B : Alignment of Rho N- and C-terminal amino acid sequences across species. Numbering is based on the predicted zebrafish rh1–1 . The conserved N-linked glycosylation sequence and VXPX targeting sequence are underlined. C : DNA sequence and amino acid overlay of zebrafish rh1–1 5′- (left) and 3′- (right) coding sequences. Blue text represents the CRISPR PAM sequence. Underlined DNA represents the target for guide RNA hybridization. The purple overscore represent restriction sites for Nci I or Bbs I. Arrows indicate predicted Cas9 cleavage sites. D : Diagrammatic representation of the 253-bp 5′- rh1–1 gene amplicon and the predicted products following digestion with Nci I. E : RFLP analysis of a 253-bp PCR product spanning the 5′ rh1–1 gene (C, undigested control) and digested with Nci I (N) shows retention of the original amplicon (arrow) from pooled DNA from injected embryos versus complete digestion of the PCR product of uninjected control DNA (arrowhead). F : Diagrammatic representation of the 325-bp 3′-amplicon and the predicted products following digestion with Bbs I. G : RFLP analysis of a 325-bp PCR product (C, undigested control) spanning the 3′ rh1–1 sequence digested with Bbs I (B). The PCR product (arrow) from uninjected-controls was digested to near completion, yielding two visible bands of 102 and 182 bp. The 284-bp band (arrowhead) following loss of the 5′- Bbs I site is clearly visible in the injected embryos relative to the controls. The following sequences were used for the RHO alignment: Danio rerio ( NP571159.1 ), X. laevis ( NP001080517.1 ), Mus musculus ( NP663358.1 ), Bos taurus ( NP001014890.1 ), and Homo sapiens ( NP000530.1 ).

    Journal: Molecular Vision

    Article Title: Targeted disruption of the endogenous zebrafish rhodopsin locus as models of rapid rod photoreceptor degeneration

    doi:

    Figure Lengend Snippet: Gene targeting of zebrafish rh1-1 . A : Schematic representation of the zebrafish rh1-1 locus characterized by a single exon encoding 354 amino acid proteins and 5′ and 3′ UTRs. B : Alignment of Rho N- and C-terminal amino acid sequences across species. Numbering is based on the predicted zebrafish rh1–1 . The conserved N-linked glycosylation sequence and VXPX targeting sequence are underlined. C : DNA sequence and amino acid overlay of zebrafish rh1–1 5′- (left) and 3′- (right) coding sequences. Blue text represents the CRISPR PAM sequence. Underlined DNA represents the target for guide RNA hybridization. The purple overscore represent restriction sites for Nci I or Bbs I. Arrows indicate predicted Cas9 cleavage sites. D : Diagrammatic representation of the 253-bp 5′- rh1–1 gene amplicon and the predicted products following digestion with Nci I. E : RFLP analysis of a 253-bp PCR product spanning the 5′ rh1–1 gene (C, undigested control) and digested with Nci I (N) shows retention of the original amplicon (arrow) from pooled DNA from injected embryos versus complete digestion of the PCR product of uninjected control DNA (arrowhead). F : Diagrammatic representation of the 325-bp 3′-amplicon and the predicted products following digestion with Bbs I. G : RFLP analysis of a 325-bp PCR product (C, undigested control) spanning the 3′ rh1–1 sequence digested with Bbs I (B). The PCR product (arrow) from uninjected-controls was digested to near completion, yielding two visible bands of 102 and 182 bp. The 284-bp band (arrowhead) following loss of the 5′- Bbs I site is clearly visible in the injected embryos relative to the controls. The following sequences were used for the RHO alignment: Danio rerio ( NP571159.1 ), X. laevis ( NP001080517.1 ), Mus musculus ( NP663358.1 ), Bos taurus ( NP001014890.1 ), and Homo sapiens ( NP000530.1 ).

    Article Snippet: For the restriction fragment length polymorphism (RFLP) analysis of 5′ rh1-1 , a 253-bp product was amplified (forward primer: 5′ ACA GTC CTG CCC AGA CAT CTA 3′; reverse primer: 5′ ATG GTG ACG TAC AGC GTG AG 3′) and digested with Nci I ( New England Biolabs , Ipswich, MA).

    Techniques: Sequencing, CRISPR, Hybridization, Amplification, Polymerase Chain Reaction, Injection

    Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red

    Journal: Blood

    Article Title: CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes

    doi: 10.1182/blood-2015-10-675751

    Figure Lengend Snippet: Conversion of iPSCs from Pl A1 to Pl A2 . (A) Genomic DNA, isolated from iPSCs that had been transfected with px462-gRNA1, px462-gRNA2, and Pl A2 -ssODN, was PCR amplified and digested with NciI, which differentiates the Pl A1 allelic isoform from Pl A2 . Red

    Article Snippet: PCR products were purified using QiaQuick Spin Column, digested with NciI (New England Biolabs Inc., Ipswich, MA) and analyzed on 2% agarose gels.

    Techniques: Isolation, Transfection, Polymerase Chain Reaction, Amplification

    Presence of the mutation within the index family. A: Pedigree of the family, with the filled symbols representing the affected members and the proband indicated by an arrow . B: Restriction analysis with Nci I; mutation present in the mother and daughter

    Journal:

    Article Title: A Novel Missense Mutation in the Second Extracellular Domain of GJB2, p.Ser183Phe, Causes a Syndrome of Focal Palmoplantar Keratoderma with Deafness

    doi: 10.2353/ajpath.2008.080049

    Figure Lengend Snippet: Presence of the mutation within the index family. A: Pedigree of the family, with the filled symbols representing the affected members and the proband indicated by an arrow . B: Restriction analysis with Nci I; mutation present in the mother and daughter

    Article Snippet: The mutation eliminates an Nci I (New England Biolabs, Frankfurt am Main, Germany) restriction site (the wild type contains two Nci I sites).

    Techniques: Mutagenesis

    Types of restriction profiles of blaA with NciI (Lanes 1–2) and HaeIII (Lanes 3–5), and blaB with HaeIII (Lanes 6–7) and RsaI (Lanes 8–9). PCR amplification and restriction analysis were carried out for 49 strains of Y. intermedia and Y. frederiksenii . M, molecular weight DNA marker (100 bp DNA ladder)

    Journal: BMC Microbiology

    Article Title: Characteristics of ?-lactamases and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii

    doi: 10.1186/1471-2180-7-25

    Figure Lengend Snippet: Types of restriction profiles of blaA with NciI (Lanes 1–2) and HaeIII (Lanes 3–5), and blaB with HaeIII (Lanes 6–7) and RsaI (Lanes 8–9). PCR amplification and restriction analysis were carried out for 49 strains of Y. intermedia and Y. frederiksenii . M, molecular weight DNA marker (100 bp DNA ladder)

    Article Snippet: PCR-Restriction Fragment Length Polymorphisms of bla genes PCR-RFLP of blaA was carried out with NciI and HaeIII, and that of blaB with HaeIII and RsaI (New England Biolabs Inc., Schwalbach, Germany) as per manufacturer's instructions.

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight, Marker