nbqx  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs nbqx
    Addition of DL-AP5 attenuates volume-related effects on neuronal excitability. (A) Generally, the amount of holding current to maintain voltage-clamp at –70 mV was reduced in ∧ [K + ] o + mannitol compared to ∧ [K + ] o alone in all experiments. Holding currents for <t>mixed</t> <t>AMPA</t> + NMDA receptor experiments (blue, n = 9) and those isolating NMDA receptor currents <t>(+NBQX)</t> (green, n = 13) appeared remarkably similar and had no points during which they were significantly different. Holding currents recorded during the NMDA receptor inhibition experiments (+NBQX/+DL-AP5) were less negative overall and were significantly less negative following the second ∧ [K + ] o + mannitol co-application period (yellow, n = 8). (B) Comparison of resting membrane potentials for the NMDA receptor isolation and NMDA receptor inhibition experiments. Shifts in resting membrane potential indicated that cells became depolarized relative to baseline during application of ∧ [K + ] o , while co-application of ∧ [K + ] o + mannitol triggered slight hyperpolarizing shifts. Resting membrane potentials recorded during the NMDAR inhibition experiments (yellow, n = 8) indicated significantly less depolarization compared to the NMDAR isolation experiments following the initial application of ∧ [K + ] o alone, the mannitol wash period, and the second co-application of ∧ [K + ] o + mannitol (green, n = 13). * p
    Nbqx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nbqx/product/Alomone Labs
    Average 94 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    nbqx - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Contributions of Astrocyte and Neuronal Volume to CA1 Neuron Excitability Changes in Elevated Extracellular Potassium"

    Article Title: Contributions of Astrocyte and Neuronal Volume to CA1 Neuron Excitability Changes in Elevated Extracellular Potassium

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2022.930384

    Addition of DL-AP5 attenuates volume-related effects on neuronal excitability. (A) Generally, the amount of holding current to maintain voltage-clamp at –70 mV was reduced in ∧ [K + ] o + mannitol compared to ∧ [K + ] o alone in all experiments. Holding currents for mixed AMPA + NMDA receptor experiments (blue, n = 9) and those isolating NMDA receptor currents (+NBQX) (green, n = 13) appeared remarkably similar and had no points during which they were significantly different. Holding currents recorded during the NMDA receptor inhibition experiments (+NBQX/+DL-AP5) were less negative overall and were significantly less negative following the second ∧ [K + ] o + mannitol co-application period (yellow, n = 8). (B) Comparison of resting membrane potentials for the NMDA receptor isolation and NMDA receptor inhibition experiments. Shifts in resting membrane potential indicated that cells became depolarized relative to baseline during application of ∧ [K + ] o , while co-application of ∧ [K + ] o + mannitol triggered slight hyperpolarizing shifts. Resting membrane potentials recorded during the NMDAR inhibition experiments (yellow, n = 8) indicated significantly less depolarization compared to the NMDAR isolation experiments following the initial application of ∧ [K + ] o alone, the mannitol wash period, and the second co-application of ∧ [K + ] o + mannitol (green, n = 13). * p
    Figure Legend Snippet: Addition of DL-AP5 attenuates volume-related effects on neuronal excitability. (A) Generally, the amount of holding current to maintain voltage-clamp at –70 mV was reduced in ∧ [K + ] o + mannitol compared to ∧ [K + ] o alone in all experiments. Holding currents for mixed AMPA + NMDA receptor experiments (blue, n = 9) and those isolating NMDA receptor currents (+NBQX) (green, n = 13) appeared remarkably similar and had no points during which they were significantly different. Holding currents recorded during the NMDA receptor inhibition experiments (+NBQX/+DL-AP5) were less negative overall and were significantly less negative following the second ∧ [K + ] o + mannitol co-application period (yellow, n = 8). (B) Comparison of resting membrane potentials for the NMDA receptor isolation and NMDA receptor inhibition experiments. Shifts in resting membrane potential indicated that cells became depolarized relative to baseline during application of ∧ [K + ] o , while co-application of ∧ [K + ] o + mannitol triggered slight hyperpolarizing shifts. Resting membrane potentials recorded during the NMDAR inhibition experiments (yellow, n = 8) indicated significantly less depolarization compared to the NMDAR isolation experiments following the initial application of ∧ [K + ] o alone, the mannitol wash period, and the second co-application of ∧ [K + ] o + mannitol (green, n = 13). * p

    Techniques Used: Inhibition, Isolation

    DL-AP5 substantially attenuates NMDA receptor currents during application of ∧ [K + ] o . Approximately 1-min section of recording taken from the mannitol wash period for an experiment conducted in NBQX without DL-AP5 (A) compared to the mannitol wash period with 50 μM DL-AP5 (B) . Note the number of large SIC-like events in the absence, but not the presence, of DL-AP5. (C) Frequency of all NMDA receptor events was significantly lower in DL-AP5 (yellow, n = 8) compared to + NBQX alone (green, n = 13) during ∧ [K + ] o application both with and without mannitol. (D) When separating events into individual 10-min recording periods, NMDA receptor events were significantly reduced during the mannitol wash period and second ∧ [K + ] o + mannitol application period. (E) DL-AP5 also significantly inhibited the occurrence of SICs either with or without mannitol. (F) When grouping frequencies of SICs into 10-min time bins, DL-AP5 significantly inhibited SICs during the mannitol wash period and second ∧ [K + ] o + mannitol co-application period. SICs were blocked completely during the second ∧ [K + ] o + mannitol co-application period. * p
    Figure Legend Snippet: DL-AP5 substantially attenuates NMDA receptor currents during application of ∧ [K + ] o . Approximately 1-min section of recording taken from the mannitol wash period for an experiment conducted in NBQX without DL-AP5 (A) compared to the mannitol wash period with 50 μM DL-AP5 (B) . Note the number of large SIC-like events in the absence, but not the presence, of DL-AP5. (C) Frequency of all NMDA receptor events was significantly lower in DL-AP5 (yellow, n = 8) compared to + NBQX alone (green, n = 13) during ∧ [K + ] o application both with and without mannitol. (D) When separating events into individual 10-min recording periods, NMDA receptor events were significantly reduced during the mannitol wash period and second ∧ [K + ] o + mannitol application period. (E) DL-AP5 also significantly inhibited the occurrence of SICs either with or without mannitol. (F) When grouping frequencies of SICs into 10-min time bins, DL-AP5 significantly inhibited SICs during the mannitol wash period and second ∧ [K + ] o + mannitol co-application period. SICs were blocked completely during the second ∧ [K + ] o + mannitol co-application period. * p

    Techniques Used:

    2) Product Images from "NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection"

    Article Title: NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection

    Journal: Experimental neurology

    doi: 10.1016/j.expneurol.2016.04.010

    Gliosis within the hippocampus and dentate gyrus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. Gliosis was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p
    Figure Legend Snippet: Gliosis within the hippocampus and dentate gyrus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. Gliosis was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p

    Techniques Used: Mouse Assay, Infection

    Weight change in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were weighed daily through day 21 post infection. Data represents percent of daily weight in comparison to weight at day −1, given as mean ± standard error of the mean (SEM) for groups of 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p
    Figure Legend Snippet: Weight change in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were weighed daily through day 21 post infection. Data represents percent of daily weight in comparison to weight at day −1, given as mean ± standard error of the mean (SEM) for groups of 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p

    Techniques Used: Mouse Assay, Infection

    Mortality in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were monitored through day 21 post infection. Data represents percent daily survival in comparison to day 0. The numbers of mice per group at day 0 were 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p
    Figure Legend Snippet: Mortality in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were monitored through day 21 post infection. Data represents percent daily survival in comparison to day 0. The numbers of mice per group at day 0 were 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p

    Techniques Used: Mouse Assay, Infection

    Neuronal cell loss within the hippocampus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. A. Neuronal cell loss was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p
    Figure Legend Snippet: Neuronal cell loss within the hippocampus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. A. Neuronal cell loss was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p

    Techniques Used: Mouse Assay, Infection

    3) Product Images from "Expression of TRPV1 channels by Cajal‐Retzius cells and layer‐specific modulation of synaptic transmission by capsaicin in the mouse hippocampus"

    Article Title: Expression of TRPV1 channels by Cajal‐Retzius cells and layer‐specific modulation of synaptic transmission by capsaicin in the mouse hippocampus

    Journal: The Journal of Physiology

    doi: 10.1113/JP275685

    Responses to capsaicin are not dependent on the integrity of synaptic transmission and/or action potential generation A , left panel, control image in the presence of antagonists of synaptic ionotropic receptors (syn blockers; NBQX, 20 μM; D‐AP5, 50 μM; picrotoxinin, 50 μM) and after the addition of capsaicin (1 μM). Middle panel, similar to left panel with the additional constant presence of TTX. Right panel, control image in the presence of TTX and cadmium (150 μM) and after the introduction of capsaicin (1 μM). Notice that none of the experimental conditions was successful in preventing responses to capsaicin. Colour scale bar in arbitrary units. B , F / F 0 traces obtained from several CRs shown in different colours and superimposed in the same experimental conditions of the panels in A . C , summary plots from several experiments quantifying the peak of the F / F 0 response (left), its half‐width (middle) and latency (right).
    Figure Legend Snippet: Responses to capsaicin are not dependent on the integrity of synaptic transmission and/or action potential generation A , left panel, control image in the presence of antagonists of synaptic ionotropic receptors (syn blockers; NBQX, 20 μM; D‐AP5, 50 μM; picrotoxinin, 50 μM) and after the addition of capsaicin (1 μM). Middle panel, similar to left panel with the additional constant presence of TTX. Right panel, control image in the presence of TTX and cadmium (150 μM) and after the introduction of capsaicin (1 μM). Notice that none of the experimental conditions was successful in preventing responses to capsaicin. Colour scale bar in arbitrary units. B , F / F 0 traces obtained from several CRs shown in different colours and superimposed in the same experimental conditions of the panels in A . C , summary plots from several experiments quantifying the peak of the F / F 0 response (left), its half‐width (middle) and latency (right).

    Techniques Used: Transmission Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs nbqx
    Addition of DL-AP5 attenuates volume-related effects on neuronal excitability. (A) Generally, the amount of holding current to maintain voltage-clamp at –70 mV was reduced in ∧ [K + ] o + mannitol compared to ∧ [K + ] o alone in all experiments. Holding currents for <t>mixed</t> <t>AMPA</t> + NMDA receptor experiments (blue, n = 9) and those isolating NMDA receptor currents <t>(+NBQX)</t> (green, n = 13) appeared remarkably similar and had no points during which they were significantly different. Holding currents recorded during the NMDA receptor inhibition experiments (+NBQX/+DL-AP5) were less negative overall and were significantly less negative following the second ∧ [K + ] o + mannitol co-application period (yellow, n = 8). (B) Comparison of resting membrane potentials for the NMDA receptor isolation and NMDA receptor inhibition experiments. Shifts in resting membrane potential indicated that cells became depolarized relative to baseline during application of ∧ [K + ] o , while co-application of ∧ [K + ] o + mannitol triggered slight hyperpolarizing shifts. Resting membrane potentials recorded during the NMDAR inhibition experiments (yellow, n = 8) indicated significantly less depolarization compared to the NMDAR isolation experiments following the initial application of ∧ [K + ] o alone, the mannitol wash period, and the second co-application of ∧ [K + ] o + mannitol (green, n = 13). * p
    Nbqx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nbqx/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nbqx - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Addition of DL-AP5 attenuates volume-related effects on neuronal excitability. (A) Generally, the amount of holding current to maintain voltage-clamp at –70 mV was reduced in ∧ [K + ] o + mannitol compared to ∧ [K + ] o alone in all experiments. Holding currents for mixed AMPA + NMDA receptor experiments (blue, n = 9) and those isolating NMDA receptor currents (+NBQX) (green, n = 13) appeared remarkably similar and had no points during which they were significantly different. Holding currents recorded during the NMDA receptor inhibition experiments (+NBQX/+DL-AP5) were less negative overall and were significantly less negative following the second ∧ [K + ] o + mannitol co-application period (yellow, n = 8). (B) Comparison of resting membrane potentials for the NMDA receptor isolation and NMDA receptor inhibition experiments. Shifts in resting membrane potential indicated that cells became depolarized relative to baseline during application of ∧ [K + ] o , while co-application of ∧ [K + ] o + mannitol triggered slight hyperpolarizing shifts. Resting membrane potentials recorded during the NMDAR inhibition experiments (yellow, n = 8) indicated significantly less depolarization compared to the NMDAR isolation experiments following the initial application of ∧ [K + ] o alone, the mannitol wash period, and the second co-application of ∧ [K + ] o + mannitol (green, n = 13). * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Contributions of Astrocyte and Neuronal Volume to CA1 Neuron Excitability Changes in Elevated Extracellular Potassium

    doi: 10.3389/fncel.2022.930384

    Figure Lengend Snippet: Addition of DL-AP5 attenuates volume-related effects on neuronal excitability. (A) Generally, the amount of holding current to maintain voltage-clamp at –70 mV was reduced in ∧ [K + ] o + mannitol compared to ∧ [K + ] o alone in all experiments. Holding currents for mixed AMPA + NMDA receptor experiments (blue, n = 9) and those isolating NMDA receptor currents (+NBQX) (green, n = 13) appeared remarkably similar and had no points during which they were significantly different. Holding currents recorded during the NMDA receptor inhibition experiments (+NBQX/+DL-AP5) were less negative overall and were significantly less negative following the second ∧ [K + ] o + mannitol co-application period (yellow, n = 8). (B) Comparison of resting membrane potentials for the NMDA receptor isolation and NMDA receptor inhibition experiments. Shifts in resting membrane potential indicated that cells became depolarized relative to baseline during application of ∧ [K + ] o , while co-application of ∧ [K + ] o + mannitol triggered slight hyperpolarizing shifts. Resting membrane potentials recorded during the NMDAR inhibition experiments (yellow, n = 8) indicated significantly less depolarization compared to the NMDAR isolation experiments following the initial application of ∧ [K + ] o alone, the mannitol wash period, and the second co-application of ∧ [K + ] o + mannitol (green, n = 13). * p

    Article Snippet: Additional experiments in ∧ [K+ ]o ACSF + mannitol as described above were performed in the presence of 10 μM NBQX (Alomone Labs) to block AMPA receptor currents (NMDA receptor isolation), or with the addition of 50 μL DL-AP5 (Abcam) to block remaining NMDA receptor currents (NMDA receptor inhibition).

    Techniques: Inhibition, Isolation

    DL-AP5 substantially attenuates NMDA receptor currents during application of ∧ [K + ] o . Approximately 1-min section of recording taken from the mannitol wash period for an experiment conducted in NBQX without DL-AP5 (A) compared to the mannitol wash period with 50 μM DL-AP5 (B) . Note the number of large SIC-like events in the absence, but not the presence, of DL-AP5. (C) Frequency of all NMDA receptor events was significantly lower in DL-AP5 (yellow, n = 8) compared to + NBQX alone (green, n = 13) during ∧ [K + ] o application both with and without mannitol. (D) When separating events into individual 10-min recording periods, NMDA receptor events were significantly reduced during the mannitol wash period and second ∧ [K + ] o + mannitol application period. (E) DL-AP5 also significantly inhibited the occurrence of SICs either with or without mannitol. (F) When grouping frequencies of SICs into 10-min time bins, DL-AP5 significantly inhibited SICs during the mannitol wash period and second ∧ [K + ] o + mannitol co-application period. SICs were blocked completely during the second ∧ [K + ] o + mannitol co-application period. * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Contributions of Astrocyte and Neuronal Volume to CA1 Neuron Excitability Changes in Elevated Extracellular Potassium

    doi: 10.3389/fncel.2022.930384

    Figure Lengend Snippet: DL-AP5 substantially attenuates NMDA receptor currents during application of ∧ [K + ] o . Approximately 1-min section of recording taken from the mannitol wash period for an experiment conducted in NBQX without DL-AP5 (A) compared to the mannitol wash period with 50 μM DL-AP5 (B) . Note the number of large SIC-like events in the absence, but not the presence, of DL-AP5. (C) Frequency of all NMDA receptor events was significantly lower in DL-AP5 (yellow, n = 8) compared to + NBQX alone (green, n = 13) during ∧ [K + ] o application both with and without mannitol. (D) When separating events into individual 10-min recording periods, NMDA receptor events were significantly reduced during the mannitol wash period and second ∧ [K + ] o + mannitol application period. (E) DL-AP5 also significantly inhibited the occurrence of SICs either with or without mannitol. (F) When grouping frequencies of SICs into 10-min time bins, DL-AP5 significantly inhibited SICs during the mannitol wash period and second ∧ [K + ] o + mannitol co-application period. SICs were blocked completely during the second ∧ [K + ] o + mannitol co-application period. * p

    Article Snippet: Additional experiments in ∧ [K+ ]o ACSF + mannitol as described above were performed in the presence of 10 μM NBQX (Alomone Labs) to block AMPA receptor currents (NMDA receptor isolation), or with the addition of 50 μL DL-AP5 (Abcam) to block remaining NMDA receptor currents (NMDA receptor inhibition).

    Techniques:

    Gliosis within the hippocampus and dentate gyrus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. Gliosis was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p

    Journal: Experimental neurology

    Article Title: NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection

    doi: 10.1016/j.expneurol.2016.04.010

    Figure Lengend Snippet: Gliosis within the hippocampus and dentate gyrus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. Gliosis was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p

    Article Snippet: TMEV-infected mice were treated, via intraperitoneal (i.p.) injection, with MK 801 (1 mg/kg twice daily, Sigma, St. Louis, MO), GYKI-52466 (10 mg/kg twice daily, Sigma) ( ) or NBQX (approximately 22.5 mg/kg twice daily, Alomone Labs, Jerusalem, Israel) , all in a 25 μl volume, starting on day 2.5 p.i. and stopping on day 10.5 p.i.

    Techniques: Mouse Assay, Infection

    Weight change in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were weighed daily through day 21 post infection. Data represents percent of daily weight in comparison to weight at day −1, given as mean ± standard error of the mean (SEM) for groups of 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p

    Journal: Experimental neurology

    Article Title: NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection

    doi: 10.1016/j.expneurol.2016.04.010

    Figure Lengend Snippet: Weight change in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were weighed daily through day 21 post infection. Data represents percent of daily weight in comparison to weight at day −1, given as mean ± standard error of the mean (SEM) for groups of 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p

    Article Snippet: TMEV-infected mice were treated, via intraperitoneal (i.p.) injection, with MK 801 (1 mg/kg twice daily, Sigma, St. Louis, MO), GYKI-52466 (10 mg/kg twice daily, Sigma) ( ) or NBQX (approximately 22.5 mg/kg twice daily, Alomone Labs, Jerusalem, Israel) , all in a 25 μl volume, starting on day 2.5 p.i. and stopping on day 10.5 p.i.

    Techniques: Mouse Assay, Infection

    Mortality in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were monitored through day 21 post infection. Data represents percent daily survival in comparison to day 0. The numbers of mice per group at day 0 were 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p

    Journal: Experimental neurology

    Article Title: NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection

    doi: 10.1016/j.expneurol.2016.04.010

    Figure Lengend Snippet: Mortality in antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were monitored through day 21 post infection. Data represents percent daily survival in comparison to day 0. The numbers of mice per group at day 0 were 20 mice (MK 801, GYKI-52466), 19 mice (NBQX) and 60 mice (PBS). †p

    Article Snippet: TMEV-infected mice were treated, via intraperitoneal (i.p.) injection, with MK 801 (1 mg/kg twice daily, Sigma, St. Louis, MO), GYKI-52466 (10 mg/kg twice daily, Sigma) ( ) or NBQX (approximately 22.5 mg/kg twice daily, Alomone Labs, Jerusalem, Israel) , all in a 25 μl volume, starting on day 2.5 p.i. and stopping on day 10.5 p.i.

    Techniques: Mouse Assay, Infection

    Neuronal cell loss within the hippocampus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. A. Neuronal cell loss was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p

    Journal: Experimental neurology

    Article Title: NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection

    doi: 10.1016/j.expneurol.2016.04.010

    Figure Lengend Snippet: Neuronal cell loss within the hippocampus of antagonist-treated mice. TMEV-infected C57BL/6J mice were treated with MK 801, GYKI-52466 and NBQX or with PBS as a control (treatment: day 2.5–10.5 post infection). Mice were sacrificed on day 21 post infection. A. Neuronal cell loss was scored as described in the Methods. The number of mice in each group is given as N above each column. Data is given as mean + SEM. †p

    Article Snippet: TMEV-infected mice were treated, via intraperitoneal (i.p.) injection, with MK 801 (1 mg/kg twice daily, Sigma, St. Louis, MO), GYKI-52466 (10 mg/kg twice daily, Sigma) ( ) or NBQX (approximately 22.5 mg/kg twice daily, Alomone Labs, Jerusalem, Israel) , all in a 25 μl volume, starting on day 2.5 p.i. and stopping on day 10.5 p.i.

    Techniques: Mouse Assay, Infection

    Responses to capsaicin are not dependent on the integrity of synaptic transmission and/or action potential generation A , left panel, control image in the presence of antagonists of synaptic ionotropic receptors (syn blockers; NBQX, 20 μM; D‐AP5, 50 μM; picrotoxinin, 50 μM) and after the addition of capsaicin (1 μM). Middle panel, similar to left panel with the additional constant presence of TTX. Right panel, control image in the presence of TTX and cadmium (150 μM) and after the introduction of capsaicin (1 μM). Notice that none of the experimental conditions was successful in preventing responses to capsaicin. Colour scale bar in arbitrary units. B , F / F 0 traces obtained from several CRs shown in different colours and superimposed in the same experimental conditions of the panels in A . C , summary plots from several experiments quantifying the peak of the F / F 0 response (left), its half‐width (middle) and latency (right).

    Journal: The Journal of Physiology

    Article Title: Expression of TRPV1 channels by Cajal‐Retzius cells and layer‐specific modulation of synaptic transmission by capsaicin in the mouse hippocampus

    doi: 10.1113/JP275685

    Figure Lengend Snippet: Responses to capsaicin are not dependent on the integrity of synaptic transmission and/or action potential generation A , left panel, control image in the presence of antagonists of synaptic ionotropic receptors (syn blockers; NBQX, 20 μM; D‐AP5, 50 μM; picrotoxinin, 50 μM) and after the addition of capsaicin (1 μM). Middle panel, similar to left panel with the additional constant presence of TTX. Right panel, control image in the presence of TTX and cadmium (150 μM) and after the introduction of capsaicin (1 μM). Notice that none of the experimental conditions was successful in preventing responses to capsaicin. Colour scale bar in arbitrary units. B , F / F 0 traces obtained from several CRs shown in different colours and superimposed in the same experimental conditions of the panels in A . C , summary plots from several experiments quantifying the peak of the F / F 0 response (left), its half‐width (middle) and latency (right).

    Article Snippet: D‐AP5, NBQX, QX314‐Cl, and TTX were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Transmission Assay