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Implen nanophotometer spectrophotometer
Nanophotometer Spectrophotometer, supplied by Implen, used in various techniques. Bioz Stars score: 99/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nanophotometer spectrophotometer/product/Implen
Average 99 stars, based on 456 article reviews
Price from $9.99 to $1999.99
nanophotometer spectrophotometer - by Bioz Stars, 2019-12
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Stable Transfection:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: 2.6 RNA of HepG2 cells stably expressing wild‐type (WT) or P24A mutant HDGF was extracted for RNA‐Seq analysis. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Synthesized:

Article Title: Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis
Article Snippet: Purity was evaluated using a NanoPhotometer spectrophotometer (IMPLEN, CA, U.S.). .. Purity was evaluated using a NanoPhotometer spectrophotometer (IMPLEN, CA, U.S.).

Construct:

Article Title: Transcriptomic profiling for prolonged drought in Dendrobium catenatum
Article Snippet: RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA). .. RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA).

Incubation:

Article Title: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach
Article Snippet: For inoculation of these media, a 0.1 mL spore suspension of Asp-4 in 0.9% NaCl (≥ 1 × 103 spores/ mL) from the −80 °C freezer stock was placed on a PDA starter plate and incubated at 28 °C. .. RNA quality was determined with a NanoPhotometer® spectrophotometer (IMPLEN, Inc., Westlake Village, CA).

Article Title: Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes
Article Snippet: The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm. .. The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm.

Expressing:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: 2.6 RNA of HepG2 cells stably expressing wild‐type (WT) or P24A mutant HDGF was extracted for RNA‐Seq analysis. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Article Title: Analysis of lncRNA Expression in Patients With Eosinophilic and Neutrophilic Asthma Focusing on LNC_000127
Article Snippet: Paragraph title: Whole Genome Gene Expression Analysis (RNA Isolation, Library Preparation, Sequencing, and Data Analysis) ... RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen, München, Germany).

Transfection:

Article Title: Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
Article Snippet: Transcriptome high-throughput sequencing was performed in the control group (SH-SY5Y cells transfected with liposome alone, Samples-ID: T04, T05, T06) and the treatment group (SH-SY5Y cells transfected with ZBTB38 siRNA, Samples-ID: T01, T02, T07). .. RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Cell Culture:

Article Title: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach
Article Snippet: Mycelia of isolate Asp-4 cultured for 48 h at 28 °C on five petri dishes containing crushed sclerotia were collected and combined and mycelia from Asp-4 cultured 48 h at 28 °C on five petri dishes containing the PDA control were collected and combined. .. RNA quality was determined with a NanoPhotometer® spectrophotometer (IMPLEN, Inc., Westlake Village, CA).

Digital PCR:

Article Title: Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes
Article Snippet: Paragraph title: Droplet digital PCR ... The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm.

Generated:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA). .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Article Title: Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
Article Snippet: RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA). .. RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: The Whole Transcriptome Involved in Denervated Muscle Atrophy Following Peripheral Nerve Injury
Article Snippet: A NanoPhotometer spectrophotometer (Implen; Westlake Village, CA, United States) was used to determine the quantity of purified RNA by measuring the absorbance at 260/280 nm. .. A NanoPhotometer spectrophotometer (Implen; Westlake Village, CA, United States) was used to determine the quantity of purified RNA by measuring the absorbance at 260/280 nm.

Sequencing:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA). .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Article Title: Transcriptomic profiling for prolonged drought in Dendrobium catenatum
Article Snippet: Paragraph title: RNA isolation and sequencing ... RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA).

Article Title: Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
Article Snippet: Paragraph title: RNA preparation and library construction for transcriptome sequencing ... RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: The Whole Transcriptome Involved in Denervated Muscle Atrophy Following Peripheral Nerve Injury
Article Snippet: Paragraph title: RNA Isolation and Sequencing Library Preparation ... A NanoPhotometer spectrophotometer (Implen; Westlake Village, CA, United States) was used to determine the quantity of purified RNA by measuring the absorbance at 260/280 nm.

Article Title: Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress
Article Snippet: The total RNA isolated from the leaves and from the root of a single plant was pooled in equal volume and concentration to prepare sequencing libraries. .. RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Effect on macrophage proliferation of a novel polysaccharide from Lactarius deliciosus (L. ex Fr.) Gray
Article Snippet: Paragraph title: RNA extraction, library preparation and high-throughput sequencing ... RNA purity was analyzed at a wavelength of 260 nm using a NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Analysis of lncRNA Expression in Patients With Eosinophilic and Neutrophilic Asthma Focusing on LNC_000127
Article Snippet: Paragraph title: Whole Genome Gene Expression Analysis (RNA Isolation, Library Preparation, Sequencing, and Data Analysis) ... RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen, München, Germany).

Article Title: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach
Article Snippet: Paragraph title: RNA isolation from Asp-4, cDNA library preparation, and transcriptome sequencing ... RNA quality was determined with a NanoPhotometer® spectrophotometer (IMPLEN, Inc., Westlake Village, CA).

Article Title: Analysis of miRNAs and their target genes in five Melilotus albus NILs with different coumarin content
Article Snippet: Paragraph title: RNA isolation, small RNA and mRNA library construction and sequencing ... And the total RNA quantity and purity were determined with the OD260/280 ratio and checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

DNA Extraction:

Article Title: Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes
Article Snippet: DNA extraction from MNCs and ATs (SAT, Mes and GO) was carried out using commercial QIAamp DNA Mini Kit according to the manufacturer’s protocol (Qiagen, USA). .. The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm.

Nucleic Acid Electrophoresis:

Article Title: Comparative Transcriptome Analysis of Male and Female Conelets and Development of Microsatellite Markers in Pinus bungeana, an Endemic Conifer in China
Article Snippet: The TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) was used to extract the total RNA of the samples, according to the manufacturer’s instructions. .. The quantity and quality of RNA were assessed by 1% gel electrophoresis and NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA). .. The RNA integrity was accurately assessed by the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA).

RNA Sequencing Assay:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: Paragraph title: RNA‐sequencing (RNA‐Seq) analysis ... RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Article Title: Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress
Article Snippet: In total, twelve RNA sequencing libraries were prepared (three each GFD, GFC, YYD, and YYC; three independent biological replicates were prepared for each treatment of each species in this study). .. RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Analysis of lncRNA Expression in Patients With Eosinophilic and Neutrophilic Asthma Focusing on LNC_000127
Article Snippet: Two milliliters were used for RNA-seq analysis. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen, München, Germany).

Mutagenesis:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: 2.6 RNA of HepG2 cells stably expressing wild‐type (WT) or P24A mutant HDGF was extracted for RNA‐Seq analysis. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Isolation:

Article Title: Transcriptomic profiling for prolonged drought in Dendrobium catenatum
Article Snippet: Paragraph title: RNA isolation and sequencing ... RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA).

Article Title: Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
Article Snippet: Total RNA was isolated from SH-SY5Y cells using TRIzol and the pure-link RNA mini kit (ThermoFisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions. .. RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: The Whole Transcriptome Involved in Denervated Muscle Atrophy Following Peripheral Nerve Injury
Article Snippet: Paragraph title: RNA Isolation and Sequencing Library Preparation ... A NanoPhotometer spectrophotometer (Implen; Westlake Village, CA, United States) was used to determine the quantity of purified RNA by measuring the absorbance at 260/280 nm.

Article Title: Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress
Article Snippet: Paragraph title: Plant materials, drought stress treatment, and RNA isolation ... RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis
Article Snippet: Paragraph title: 4.2. RNA Isolation and cDNA Synthesis ... Purity was evaluated using a NanoPhotometer spectrophotometer (IMPLEN, CA, U.S.).

Article Title: Identification of Differentially Expressed Non-coding RNA in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs Responded to PRRSV
Article Snippet: Paragraph title: Sample preparation and RNA isolation ... RNA purity was determined using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
Article Snippet: Paragraph title: Isolation of total RNA and quality assessment ... RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

Article Title: Analysis of lncRNA Expression in Patients With Eosinophilic and Neutrophilic Asthma Focusing on LNC_000127
Article Snippet: Paragraph title: Whole Genome Gene Expression Analysis (RNA Isolation, Library Preparation, Sequencing, and Data Analysis) ... RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen, München, Germany).

Article Title: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach
Article Snippet: Paragraph title: RNA isolation from Asp-4, cDNA library preparation, and transcriptome sequencing ... RNA quality was determined with a NanoPhotometer® spectrophotometer (IMPLEN, Inc., Westlake Village, CA).

Article Title: Analysis of miRNAs and their target genes in five Melilotus albus NILs with different coumarin content
Article Snippet: Paragraph title: RNA isolation, small RNA and mRNA library construction and sequencing ... And the total RNA quantity and purity were determined with the OD260/280 ratio and checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

Article Title: Comparative Transcriptome Analysis of Male and Female Conelets and Development of Microsatellite Markers in Pinus bungeana, an Endemic Conifer in China
Article Snippet: Paragraph title: 2.2. RNA Isolation and Quality Assay ... The quantity and quality of RNA were assessed by 1% gel electrophoresis and NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

RNA Extraction:

Article Title: Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress
Article Snippet: RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA). .. RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Effect on macrophage proliferation of a novel polysaccharide from Lactarius deliciosus (L. ex Fr.) Gray
Article Snippet: Paragraph title: RNA extraction, library preparation and high-throughput sequencing ... RNA purity was analyzed at a wavelength of 260 nm using a NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Labeling:

Article Title: Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes
Article Snippet: The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm. .. The components of the ddPCR itself included a commercial PCR mixture, selected fluorescent TaqMan probes, and oligonucleotide primers (Table ).

Purification:

Article Title: The Whole Transcriptome Involved in Denervated Muscle Atrophy Following Peripheral Nerve Injury
Article Snippet: The quality of the purified RNA was assessed using a BioAnalyzer 2100 (Agilent Technologies; Santa Clara, CA, United States). .. A NanoPhotometer spectrophotometer (Implen; Westlake Village, CA, United States) was used to determine the quantity of purified RNA by measuring the absorbance at 260/280 nm. .. All of the RNA samples were stored at -80°C and were sequenced by Novogene using the Illumina platform (Beijing, China).

Article Title: Analysis of miRNAs and their target genes in five Melilotus albus NILs with different coumarin content
Article Snippet: Total RNA of six fresh leaves as a sample in each M . albus genotype was isolated using the RNAprep pure Plant RNA Purification Kit (Tiangen Biotech, Beijing, China). .. And the total RNA quantity and purity were determined with the OD260/280 ratio and checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

Polymerase Chain Reaction:

Article Title: Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes
Article Snippet: The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm. .. For this, the extracted DNA samples were pretreated with ApaI restriction endonuclease (New England BioLabs, Ipswich, MA, USA) (1 μl (10 U) ApaI, 5 μl 10x CutSmart® buffer, and ~ 30 ng DNA sample), and then the reaction mixture was incubated for 3–4 h at 37 °C.

cDNA Library Assay:

Article Title: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach
Article Snippet: Paragraph title: RNA isolation from Asp-4, cDNA library preparation, and transcriptome sequencing ... RNA quality was determined with a NanoPhotometer® spectrophotometer (IMPLEN, Inc., Westlake Village, CA).

Multiplex Assay:

Article Title: Analysis of miRNAs and their target genes in five Melilotus albus NILs with different coumarin content
Article Snippet: And the total RNA quantity and purity were determined with the OD260/280 ratio and checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). .. And the total RNA quantity and purity were determined with the OD260/280 ratio and checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

Sample Prep:

Article Title: Transcriptomic profiling for prolonged drought in Dendrobium catenatum
Article Snippet: RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA). .. RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA).

Article Title: Identification of Differentially Expressed Non-coding RNA in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs Responded to PRRSV
Article Snippet: Paragraph title: Sample preparation and RNA isolation ... RNA purity was determined using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).

Spectrophotometry:

Article Title: Role of hepatoma‐derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma
Article Snippet: RNA degradation and contamination were monitored on 1% agarose gels. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA). .. RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, Waltham, MA, USA).

Article Title: Transcriptomic profiling for prolonged drought in Dendrobium catenatum
Article Snippet: Total RNA was extracted from the samples mentioned above ( ) using the RNAprep Pure Plant Kit (No. DP441; Polysaccharides & Polyphenolics-rich; Tiangen Co. Ltd, Beijing, China; http://www.tiangen.com/ ) according to the manufacturer’s protocols. .. RNA purity was estimated using a NanoPhotometer® spectrophotometer (Implen, CA, USA). .. RNA quality was assessed using an RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Article Title: Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
Article Snippet: Total RNA was isolated from SH-SY5Y cells using TRIzol and the pure-link RNA mini kit (ThermoFisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions. .. RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA). .. RNA concentration was measured using the Qubit RNA Assay Kit in Qubit 2.0 Fluorometer (Life Technologies, Camarillo, CA, USA).

Article Title: The Whole Transcriptome Involved in Denervated Muscle Atrophy Following Peripheral Nerve Injury
Article Snippet: The quality of the purified RNA was assessed using a BioAnalyzer 2100 (Agilent Technologies; Santa Clara, CA, United States). .. A NanoPhotometer spectrophotometer (Implen; Westlake Village, CA, United States) was used to determine the quantity of purified RNA by measuring the absorbance at 260/280 nm. .. All of the RNA samples were stored at -80°C and were sequenced by Novogene using the Illumina platform (Beijing, China).

Article Title: Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress
Article Snippet: In total, twelve RNA sequencing libraries were prepared (three each GFD, GFC, YYD, and YYC; three independent biological replicates were prepared for each treatment of each species in this study). .. RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA). .. RNA concentration was measured using a Qubit® RNA Assay Kit in a Qubit® 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA).

Article Title: Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis
Article Snippet: RNA degradation and contamination were monitored on 1% agarose gels. .. Purity was evaluated using a NanoPhotometer spectrophotometer (IMPLEN, CA, U.S.). .. RNA concentration was measured using a Qubit RNA Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, CA, U.S.).

Article Title: Identification of Differentially Expressed Non-coding RNA in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs Responded to PRRSV
Article Snippet: RNA degradation and contamination were initially monitored on 1% agarose gels. .. RNA purity was determined using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). .. Then, the quantity of RNA was measured through Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA).

Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
Article Snippet: RNA degradation and contamination was monitored on 1% agarose gels. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). .. RNA concentration was measured using the Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA).

Article Title: Effect on macrophage proliferation of a novel polysaccharide from Lactarius deliciosus (L. ex Fr.) Gray
Article Snippet: Total RNA was extracted using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and the RNA quality and purity were determined using 1% agarose gels ( ). .. RNA purity was analyzed at a wavelength of 260 nm using a NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA). .. The transcriptome libraries were generated using the Illumina TruSeq™ RNA Sample Preparation kit (Illumina, Inc., San Diego, CA, USA).

Article Title: Analysis of lncRNA Expression in Patients With Eosinophilic and Neutrophilic Asthma Focusing on LNC_000127
Article Snippet: RNA was isolated using the RNeasy mini extraction kit (#A419, Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen, München, Germany). .. RNA concentration was measured using a Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA).

Article Title: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach
Article Snippet: Mycelia in liquid N2 were ground with a mortar and pestle prior to RNA isolation with TRIZOL [ ]. .. RNA quality was determined with a NanoPhotometer® spectrophotometer (IMPLEN, Inc., Westlake Village, CA). .. Integrity and quantity of RNA was assessed using an RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system according to the manufacturer’s instructions (Agilent Technologies, Santa Clara, CA).

Article Title: Analysis of miRNAs and their target genes in five Melilotus albus NILs with different coumarin content
Article Snippet: RNA degradation and contamination was monitored on 1% agarose gels. .. And the total RNA quantity and purity were determined with the OD260/280 ratio and checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). .. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Article Title: Comparative Transcriptome Analysis of Male and Female Conelets and Development of Microsatellite Markers in Pinus bungeana, an Endemic Conifer in China
Article Snippet: The TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) was used to extract the total RNA of the samples, according to the manufacturer’s instructions. .. The quantity and quality of RNA were assessed by 1% gel electrophoresis and NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA). .. The RNA integrity was accurately assessed by the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA).

Concentration Assay:

Article Title: Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress
Article Snippet: The total RNA isolated from the leaves and from the root of a single plant was pooled in equal volume and concentration to prepare sequencing libraries. .. RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using a NanoPhotometer® spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Effect on macrophage proliferation of a novel polysaccharide from Lactarius deliciosus (L. ex Fr.) Gray
Article Snippet: The LDG-A concentration used to analyze the transcriptome of RAW264.7 cells was 15 µg/ml. .. RNA purity was analyzed at a wavelength of 260 nm using a NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes
Article Snippet: DNA extraction from MNCs and ATs (SAT, Mes and GO) was carried out using commercial QIAamp DNA Mini Kit according to the manufacturer’s protocol (Qiagen, USA). .. The DNA concentration in the samples was measured with an Implen NanoPhotometer spectrophotometer at a wavelength of 260 nm. .. The quality of extracted DNA was determined from the absorption ratio at 260 and 280 nm (A260/280 > 1.8). mtDNA copy number was determined by ddPCR.

High Throughput Screening Assay:

Article Title: Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
Article Snippet: Transcriptome high-throughput sequencing was performed in the control group (SH-SY5Y cells transfected with liposome alone, Samples-ID: T04, T05, T06) and the treatment group (SH-SY5Y cells transfected with ZBTB38 siRNA, Samples-ID: T01, T02, T07). .. RNA purity was checked using the NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

Article Title: Effect on macrophage proliferation of a novel polysaccharide from Lactarius deliciosus (L. ex Fr.) Gray
Article Snippet: Paragraph title: RNA extraction, library preparation and high-throughput sequencing ... RNA purity was analyzed at a wavelength of 260 nm using a NanoPhotometer spectrophotometer (Implen, Inc., Westlake Village, CA, USA).

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    Implen nanophotometer spectrophotometer
    Nanophotometer Spectrophotometer, supplied by Implen, used in various techniques. Bioz Stars score: 99/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanophotometer spectrophotometer/product/Implen
    Average 99 stars, based on 456 article reviews
    Price from $9.99 to $1999.99
    nanophotometer spectrophotometer - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

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