nanodrop nd 1000 spectrophotometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nanodrop nd 1000 spectrophotometer
    Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) <t>NanoDrop</t> ® <t>ND-1000</t> Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.
    Nanodrop Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop nd 1000 spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 13044 article reviews
    Price from $9.99 to $1999.99
    nanodrop nd 1000 spectrophotometer - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling"

    Article Title: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-5-9

    Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) NanoDrop ® ND-1000 Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.
    Figure Legend Snippet: Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) NanoDrop ® ND-1000 Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.

    Techniques Used: Purification, Spectrophotometry

    2) Product Images from "A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue"

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152157

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Figure Legend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Techniques Used: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    3) Product Images from "A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue"

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152157

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Figure Legend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Techniques Used: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    Related Articles

    Produced:

    Article Title: A MODIFIED PROTEIN ASSAY FROM MICROGRAM TO LOW NANOGRAM LEVELS IN DILUTE SAMPLES
    Article Snippet: .. The absorbency of eluted protein samples from macroassay on Nanodrop™ spectrophotometer at 630 nm produced linear curves with small SD and high r2 values ( ). .. However, spectrophotometry was found to be less favorable for microassay as indicated by relatively lower r2 values, suggesting less than consistent protein elution from the NC membrane ( ).

    Isolation:

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Quantification of isolated extracellular vesicles was done by measuring total protein content by nanodrop spectrophotometer. .. Nanodrop observations showed the presence of extracellular vesicles by indirectly measuring the protein content of the sample as PEG precipitation would isolate extracellular vesicles.

    Spectrophotometry:

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Quantification of isolated extracellular vesicles was done by measuring total protein content by nanodrop spectrophotometer. .. Nanodrop observations showed the presence of extracellular vesicles by indirectly measuring the protein content of the sample as PEG precipitation would isolate extracellular vesicles.

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer. ..

    Article Title: A MODIFIED PROTEIN ASSAY FROM MICROGRAM TO LOW NANOGRAM LEVELS IN DILUTE SAMPLES
    Article Snippet: .. Samples eluted from the microassay on the other hand, due to its small volume size can be analyzed only by Nanodrop™ spectrophotometer. .. The absorbency of eluted protein samples from macroassay on Nanodrop™ spectrophotometer at 630 nm produced linear curves with small SD and high r2 values ( ).

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Finally, RNA was eluted and quantified with nanodrop spectrophotometer. ..

    Article Title: A MODIFIED PROTEIN ASSAY FROM MICROGRAM TO LOW NANOGRAM LEVELS IN DILUTE SAMPLES
    Article Snippet: .. The absorbency of eluted protein samples from macroassay on Nanodrop™ spectrophotometer at 630 nm produced linear curves with small SD and high r2 values ( ). .. However, spectrophotometry was found to be less favorable for microassay as indicated by relatively lower r2 values, suggesting less than consistent protein elution from the NC membrane ( ).

    Article Title: A MODIFIED PROTEIN ASSAY FROM MICROGRAM TO LOW NANOGRAM LEVELS IN DILUTE SAMPLES
    Article Snippet: .. Nanodrop™ spectrophotometer suitable for handling small size volumes (2 μl) can be used for macro as well as microassays. ..

    Article Title: Biological Effects of Functionalizing Copolymer Scaffolds with Nanodiamond Particles
    Article Snippet: .. The protein and DNA level were also measured directly on a Nanodrop® Spectrophotometer as described above. ..

    Article Title: A MODIFIED PROTEIN ASSAY FROM MICROGRAM TO LOW NANOGRAM LEVELS IN DILUTE SAMPLES
    Article Snippet: .. Samples eluted from macroassay can be analyzed individually using Nanodrop™ spectrophotometer or simultaneously (up to 96) using LT-4000 multi-well plate reader. .. Samples eluted from the microassay on the other hand, due to its small volume size can be analyzed only by Nanodrop™ spectrophotometer.

    Protein Concentration:

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer. ..

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    Thermo Fisher nano drop 1000 spectrophotometer
    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by <t>Nano-drop</t> 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P
    Nano Drop 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop 1000 spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    nano drop 1000 spectrophotometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher nanodrop spectrophotometer nd 1000
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nanodrop Spectrophotometer Nd 1000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop spectrophotometer nd 1000/product/Thermo Fisher
    Average 94 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    nanodrop spectrophotometer nd 1000 - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    85
    Thermo Fisher nano drop kit
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nano Drop Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop kit/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano drop kit - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Journal: MethodsX

    Article Title: Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction

    doi: 10.1016/j.mex.2019.10.015

    Figure Lengend Snippet: RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Article Snippet: The yield of total RNA was determined by Nano-drop 1000 Spectrophotometer (Thermo Scientific).

    Techniques: Lysis, Spectrophotometry, RNA Extraction

    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Journal: Veterinary Research

    Article Title: Visualizing bovine leukemia virus (BLV)-infected cells and measuring BLV proviral loads in the milk of BLV seropositive dams

    doi: 10.1186/s13567-019-0724-1

    Figure Lengend Snippet: Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Article Snippet: The quantity and quality of DNA samples extracted from milk sample was determined based on the A260/280 ratio using a Nanodrop Spectrophotometer ND-1000 (Thermo Fisher Scientific).

    Techniques: Infection, Spectrophotometry