nanodrop nd 1000 spectrophotometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nanodrop nd 1000 spectrophotometer
    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the <t>NanoDrop</t> <t>ND-1000</t> Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Nanodrop Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 9098 article reviews
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    nanodrop nd 1000 spectrophotometer - by Bioz Stars, 2020-01
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    Images

    1) Product Images from "A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue"

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152157

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Figure Legend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Techniques Used: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    2) Product Images from "A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue"

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152157

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.
    Figure Legend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Techniques Used: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    3) Product Images from "Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling"

    Article Title: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-5-9

    Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) NanoDrop ® ND-1000 Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.
    Figure Legend Snippet: Effect of aRNA purity and labelled-aRNA purification . (A) Graph showing coupling efficiency (C) and 320/650 (B) values for six MCF-7 aRNA samples with different 260/280 ratios. (B) Agilent Bioanalyzer pattern of MCF-7 Cy5-labelled target using an aRNA with 260/280 ratio of 1.6. Sharp spikes represent uncoupled Cy5 dye. (C) Same as B for aRNA with 260/280 ratio of 2 and coupling efficiency near 1. (D) NanoDrop ® ND-1000 Spectrophotometer absorptions at 320 and 650 nm wavelengths of a MCF-7 Cy5-labelled target. 320/650 ratio-0.6. (E) Same as in d (different sample). 320/650 ratio-0.09. (F) Recovery rates (R) and coupling efficiencies (C) for different labelled-aRNA purification methods. Data is presented for MCF-7 cell line and measurements represent an average of three separate reactions. Li-ETOH – LiCl-ethanol; PCI – phenol/chloroform/isoamyl alcohol.

    Techniques Used: Purification, Spectrophotometry

    Related Articles

    Amplification:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. 8 µg of cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 250K Nsp Assay Kit (Affymetrix, 900753).

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: A second PCR amplification introduced Illumina adapters tagged with a unique 6 bp barcode (on the P7 adapter) for each sample and time point. .. The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer.

    Article Title: A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
    Article Snippet: Expression profiling and differential expression analysis RNA quality and quantity were assessed by Nanodrop ND-1000 spectrophotometer and Agilent bioanalyzer, respectively. .. Paired RNA samples, selected based on RNA quality and quantity, were amplified and biotin labeled using the Illumina TotalPrep-96 RNA amplification kit, hybridized to Illumina HumanRef-8v3 beadarrays (Illumina), and scanned using an Illumina BeadXpress reader.

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer. .. The cDNAs from each sample were amplified for muscarinic receptors via reverse-transcriptase PCR (RT-PCR) and transcript level was quantified by qRT-PCR as previously described ( ).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. 8.5 ug of the cDNA from each sample were fragmented and labeling with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753) following the instructions of manufacturer.

    Synthesized:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. Lep, LEPR, LEPR-b, PNPLA2 , and LIPE , were amplified from cetacean, rodent and human cDNAs using specifically designed gene and species-specific primers ( Online Resource 1 ).

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: .. RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer. .. The cDNAs from each sample were amplified for muscarinic receptors via reverse-transcriptase PCR (RT-PCR) and transcript level was quantified by qRT-PCR as previously described ( ).

    Quantitative RT-PCR:

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: Paragraph title: RNA preparation, RT-PCR and qRT-PCR ... RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer.

    SYBR Green Assay:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: When the SYBR Green signal reached a plateau after 27 cycles, the reaction was stopped. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Microarray:

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Biotin-labeled cRNA (20 μg) was fragmented and hybridized to an Affymetrix Rat Genome 230 2.0 microarray overnight in the Affymetrix 640 hybridization oven with a speed of 60 rpm for 16 h. Microarrays were washed and stained using an Affymetrix Fluidics Station FS400.

    Incubation:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. Human: samples ready to hybridize were denatured at 99ºC for 2 min prior to incubation into the GeneChip Human PrimeView arrays (Affymetrix, 901838).

    Expressing:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA.

    Article Title: A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
    Article Snippet: .. Expression profiling and differential expression analysis RNA quality and quantity were assessed by Nanodrop ND-1000 spectrophotometer and Agilent bioanalyzer, respectively. .. Paired RNA samples, selected based on RNA quality and quantity, were amplified and biotin labeled using the Illumina TotalPrep-96 RNA amplification kit, hybridized to Illumina HumanRef-8v3 beadarrays (Illumina), and scanned using an Illumina BeadXpress reader.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Paragraph title: Gene Expression Profiling and data analysis ... Samples were quantified using the NanoDrop ND-1000 spectrophotometer.

    Hybridization:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. After hybridization for 16h at 45ºC, washing and staining was performed in the Affymetrix GeneAtlas Fluidics Station.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Biotin-labeled cRNA (20 μg) was fragmented and hybridized to an Affymetrix Rat Genome 230 2.0 microarray overnight in the Affymetrix 640 hybridization oven with a speed of 60 rpm for 16 h. Microarrays were washed and stained using an Affymetrix Fluidics Station FS400.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. Hybridization was performed for 16h at 45ºC / 60 rpm in the GeneChip Hybridization Oven 645 (Affymetrix, 00-0331).

    Transfection:

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: RNA preparation, RT-PCR and qRT-PCR Total RNA was isolated from six mouse sclera of each strain (Chrm2 +/+ , Chrm2 +/− , Chrm2 −/− , 6 weeks lens-treated and control WT eyes; n =6 eyes), and from human and mouse SF cell lysates with siRNA transfection and after drug treatment using the MELT™ Total Nucleic Acid Isolation System (Ambion Inc., Austin, TX) according to the manufacturer’s instructions. .. RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer.

    Concentration Assay:

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: By Flory–Huggins mean-field theory for polymer solutions, this PEG in PBS renders a simple technique for separation and concentration process (Johansson et al. ) The main criteria to isolate extracellular vesicles lies in the gentle sample processing which could finally result in the isolation of these delicate structures. .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer.

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer. .. RNA samples were selected for further cDNA library and small RNA (sRNA) library construction according to the following selection criteria: OD260/280≥1.8, OD260/230≥1.8, RIN≥9.0, 28S/18S≥1.6, a concentration of ≥200 ng/μl, and total mass of ≥30 μg.

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: .. RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer. .. The cDNAs from each sample were amplified for muscarinic receptors via reverse-transcriptase PCR (RT-PCR) and transcript level was quantified by qRT-PCR as previously described ( ).

    Polymerase Chain Reaction:

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: The PCR and purification protocols were identical to those of PCR1 except that the products from PCR1 were used as template and different primers were used. .. The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer.

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. Lep, LEPR, LEPR-b, PNPLA2 , and LIPE , were amplified from cetacean, rodent and human cDNAs using specifically designed gene and species-specific primers ( Online Resource 1 ).

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer. .. The cDNAs from each sample were amplified for muscarinic receptors via reverse-transcriptase PCR (RT-PCR) and transcript level was quantified by qRT-PCR as previously described ( ).

    Imaging:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. The arrays were scanned in the GeneAtlas Imaging Station.

    Protein Concentration:

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer. ..

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: EVs isolated with UC were also quantified using Nanodrop spectrophotometer which showed an average yield of 7.564 mg/ml of seminal plasma. .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer.

    Sequencing:

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer. .. The P7 and Index1 reads were performed with standard sequencing primers, whereas the P5 read was sequenced with a custom primer (Sol-P5_alt-PCR, ).

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer. .. For mRNA sequencing, the total RNA was cleaned up using the RNeasy Plant Mini Kit (74904; Qiagen, Valencia, CA, USA).

    Isolation:

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: Paragraph title: Quantification of isolated extracellular vesicles ... Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Paragraph title: RNA isolation, Whole Transcriptome Amplification (WTA) and microarrays processing ... Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: Paragraph title: RNA isolation/cDNA synthesis ... RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA.

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: Paragraph title: RNA isolation ... The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer.

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: Nanodrop spectrophotometer showed presence of EVs by indirectly measuring protein content of precipitated EVs. .. EVs isolated by PEG precipitation showed a wide size range from 30 to 1000 nm with Z average of 75.4 nm and a PI of 0.464, whereas ultracentrifuge sample showed size range of 60–1000 nm with Z average of 501.3 nm with a PI of 0.692.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Gene Expression Profiling and data analysis Total RNA was isolated from HEK cells as described above. .. Samples were quantified using the NanoDrop ND-1000 spectrophotometer.

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Quantification of isolated extracellular vesicles was done with nanodrop spectrophotometer (Thermo Fisher). ..

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. EVs isolated with UC were also quantified using Nanodrop spectrophotometer which showed an average yield of 7.564 mg/ml of seminal plasma. .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer.

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: RNA preparation, RT-PCR and qRT-PCR Total RNA was isolated from six mouse sclera of each strain (Chrm2 +/+ , Chrm2 +/− , Chrm2 −/− , 6 weeks lens-treated and control WT eyes; n =6 eyes), and from human and mouse SF cell lysates with siRNA transfection and after drug treatment using the MELT™ Total Nucleic Acid Isolation System (Ambion Inc., Austin, TX) according to the manufacturer’s instructions. .. RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer.

    Labeling:

    Article Title: A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
    Article Snippet: Expression profiling and differential expression analysis RNA quality and quantity were assessed by Nanodrop ND-1000 spectrophotometer and Agilent bioanalyzer, respectively. .. Paired RNA samples, selected based on RNA quality and quantity, were amplified and biotin labeled using the Illumina TotalPrep-96 RNA amplification kit, hybridized to Illumina HumanRef-8v3 beadarrays (Illumina), and scanned using an Illumina BeadXpress reader.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. 8.5 ug of the cDNA from each sample were fragmented and labeling with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753) following the instructions of manufacturer.

    Purification:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. 8 µg of cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 250K Nsp Assay Kit (Affymetrix, 900753).

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: The PCR and purification protocols were identical to those of PCR1 except that the products from PCR1 were used as template and different primers were used. .. The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer.

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer. .. For sRNA sequencing, RNA fragments of 18–30 nt were isolated and purified from total RNA samples extracted using Trizol reagent after electrophoresing on a denaturing 15% polyacrylamide gel.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: The cDNA was amplified for 17 cycles and purified using PureLink Quick PCR Purification Kit (Invitrogen, K310001). .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: Paragraph title: RNA preparation, RT-PCR and qRT-PCR ... RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer.

    Immunoprecipitation:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Library preparation and amplification were performed following the distributor’s (Sigma-Aldrich) recommendations for WTA2 from purified immunoprecipitated or diluted input RNA. .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer).

    cDNA Library Assay:

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer. .. RNA samples were selected for further cDNA library and small RNA (sRNA) library construction according to the following selection criteria: OD260/280≥1.8, OD260/230≥1.8, RIN≥9.0, 28S/18S≥1.6, a concentration of ≥200 ng/μl, and total mass of ≥30 μg.

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Human PrimeView and GeneAtlas MG-430 PM microarrays analysis cDNA library preparation and amplification were performed according to the manufacturer’s instructions (Sigma-Aldrich) for the WTA2 kit from 25 ng starting material. .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA).

    RNA Extraction:

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: .. 3.2 Analysis of RNA following RNA Extraction The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ). ..

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: Nanodrop spectrophotometer showed presence of EVs by indirectly measuring protein content of precipitated EVs. .. Total RNA extraction from PEG EVs analysed with nanodrop spectrophotometer, showed presence of RNA content in varying concentrations obtained from different ratios in nanograms.

    Selection:

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer. .. RNA samples were selected for further cDNA library and small RNA (sRNA) library construction according to the following selection criteria: OD260/280≥1.8, OD260/230≥1.8, RIN≥9.0, 28S/18S≥1.6, a concentration of ≥200 ng/μl, and total mass of ≥30 μg.

    Agarose Gel Electrophoresis:

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: .. The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer. .. Equimolar samples of all 40 libraries were combined in a single tube and sequenced using a SE50 kit on an Illumina MiSeq (Dana Farber Cancer Institute Molecular Biology Core Facility).

    Next-Generation Sequencing:

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: Paragraph title: Next-generation sequencing of populations with degeneracy introduced at UAG-positions ... The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer.

    Spectrophotometry:

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: .. 3.2 Analysis of RNA following RNA Extraction The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ). ..

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer. ..

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: .. Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. 8 µg of cDNA were subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from GeneChip Mapping 250K Nsp Assay Kit (Affymetrix, 900753).

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: .. 2.3 Analysis of RNA Quantity, Purity and Quality Samples were assessed for RNA quantity using NanoDrop ND-1000 Spectrophotometer, with the purity determined by absorbance ratio measurement at 260nm/280nm (ideal ratio of 2.0). .. Sample quality was determined from the RNA Integrity Number (RIN) on the Agilent 2100 Bioanalyser using either the Agilent RNA 6000 Nano Kit (samples greater than 25ng/μl) or Agilent RNA 6000 Pico Kit (samples less than 25ng/μl).

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: .. The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer. .. Equimolar samples of all 40 libraries were combined in a single tube and sequenced using a SE50 kit on an Illumina MiSeq (Dana Farber Cancer Institute Molecular Biology Core Facility).

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA. .. Lep, LEPR, LEPR-b, PNPLA2 , and LIPE , were amplified from cetacean, rodent and human cDNAs using specifically designed gene and species-specific primers ( Online Resource 1 ).

    Article Title: A statin-dependent QTL for GATM expression is associated with statin-induced myopathy
    Article Snippet: .. Expression profiling and differential expression analysis RNA quality and quantity were assessed by Nanodrop ND-1000 spectrophotometer and Agilent bioanalyzer, respectively. .. Paired RNA samples, selected based on RNA quality and quantity, were amplified and biotin labeled using the Illumina TotalPrep-96 RNA amplification kit, hybridized to Illumina HumanRef-8v3 beadarrays (Illumina), and scanned using an Illumina BeadXpress reader.

    Article Title: Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips
    Article Snippet: .. The quality of RNA was verified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA) and a 2100 Bioanalyzer RNA Nanochip (Agilent, Santa Clara, CA, USA), and the quantity of total RNA was determined using the NanoDrop ND-1000 Spectrophotometer. .. RNA samples were selected for further cDNA library and small RNA (sRNA) library construction according to the following selection criteria: OD260/280≥1.8, OD260/230≥1.8, RIN≥9.0, 28S/18S≥1.6, a concentration of ≥200 ng/μl, and total mass of ≥30 μg.

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Nanodrop spectrophotometer showed presence of EVs by indirectly measuring protein content of precipitated EVs. .. EVs isolated by PEG precipitation showed a wide size range from 30 to 1000 nm with Z average of 75.4 nm and a PI of 0.464, whereas ultracentrifuge sample showed size range of 60–1000 nm with Z average of 501.3 nm with a PI of 0.692.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: .. Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Double-stranded cDNA was prepared from 1 μg of total RNA using the Affymetrix cDNA synthesis kit and was then in vitro-transcribed using an IVT labeling kit (Affymetrix), with the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix).

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. Quantification of isolated extracellular vesicles was done with nanodrop spectrophotometer (Thermo Fisher). ..

    Article Title: Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab
    Article Snippet: .. EVs isolated with UC were also quantified using Nanodrop spectrophotometer which showed an average yield of 7.564 mg/ml of seminal plasma. .. Yield of EVs with PEG and UC is calculated by the average protein concentration measured with Nanodrop spectrophotometer.

    Article Title: Muscarinic cholinergic receptor (M2) plays a crucial role in the development of myopia in mice
    Article Snippet: .. RNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNAs were synthesized using iScriptTM select cDNA synthesis kit (Bio-Rad Laboratories, CA). cDNA concentration and quality were assessed by absorbance at 260 nm and the absorbance ratio of 260/280, respectively, using Nanodrop® ND-1000 Spectrophotometer. .. The cDNAs from each sample were amplified for muscarinic receptors via reverse-transcriptase PCR (RT-PCR) and transcript level was quantified by qRT-PCR as previously described ( ).

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: .. Quantification of amplified cDNA was done on a Nanodrop ND-1000 spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, USA). .. 8.5 ug of the cDNA from each sample were fragmented and labeling with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753) following the instructions of manufacturer.

    Sampling:

    Article Title: Beyond Thermoregulation: Metabolic Function of Cetacean Blubber in Migrating Bowhead and Beluga Whales
    Article Snippet: Beluga blubber was sampled in a similar fashion: superficial samples were isolated 2cm from the base of the epidermis, deep blubber was sampled 2cm from the connected hypodermis and intermediate samples were taken midway between these two sampling locations. .. RNA was quantified with triplicate reads using a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and gDNA contamination was assessed via PCR ( Online Resource 1 ) in an Eppendorf Personal minicycler (Eppendorf, Hauppauge, NY, USA). cDNAs and no reverse transcriptase controls were synthesized from extracted mRNAs and purchased human RNAs following manufacturer recommended protocols for the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), quantified based on triplicate reads on a Nanodrop® ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA) and normalized to 50ng total RNA.

    Produced:

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue
    Article Snippet: 3.2 Analysis of RNA following RNA Extraction The Livibrush cytobrush and Isohelix swab collection methods resulted in a low RNA yield and low purity as determined using the NanoDrop ND-1000 Spectrophotometer ( ). .. The papillae biopsy produced the highest RNA integrity , with a RIN average greater than 8, with the other collection techniques resulting in lower quality RNA (see for representative Bio-analyser profiles).

    Activation Assay:

    Article Title: Biocontainment of genetically modified organisms by synthetic protein design
    Article Snippet: Thermocycling (BioRad C1000 thermocycler) involved heat activation at 95 °C for 3 minutes, followed by 30 cycles of denaturation at 98 °C for 20 seconds, annealing at 62 °C for 15 seconds, and elongation at 72 °C for 30 seconds with a final elongation for 1 minute. .. The final DNA libraries were checked on a 1.5% w/v agarose gel and quantitated using a NanoDrop ND-1000 spectrophotometer.

    Staining:

    Article Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing
    Article Snippet: Amplified cDNA was purified and quantified on a Nanodrop ND-1000 spectrophotometer (Thermo-Fischer). .. After hybridization for 16h at 45ºC, washing and staining was performed in the Affymetrix GeneAtlas Fluidics Station.

    Article Title: Striatal microRNA controls cocaine intake through CREB signaling
    Article Snippet: Samples were quantified using the NanoDrop ND-1000 spectrophotometer. .. Biotin-labeled cRNA (20 μg) was fragmented and hybridized to an Affymetrix Rat Genome 230 2.0 microarray overnight in the Affymetrix 640 hybridization oven with a speed of 60 rpm for 16 h. Microarrays were washed and stained using an Affymetrix Fluidics Station FS400.

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