nanodrop nd 1000 spectrophotometer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    NanoDrop spectrophotometer
    Description:

    Catalog Number:
    ND-1000
    Price:
    None
    Score:
    85
    Buy from Supplier
    Name:
    NanoDrop 2000 packaged
    Description:
    Thermo Scientific NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0.5μL.
    Catalog Number:
    ND2000LAPTOP
    Price:
    None
    Applications:
    Industrial & Applied Science|Spectroscopy Elemental Isotope Analysis
    Category:
    Instruments and Equipment, Spectroscopy & Elemental Analysis Instruments & Equipment, UV-Vis & Vis Instrumentation
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher nanodrop nd 1000 spectrophotometer
    Thermo Scientific NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0.5μL.
    https://www.bioz.com/result/nanodrop nd 1000 spectrophotometer/product/Thermo Fisher
    Average 95 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    nanodrop nd 1000 spectrophotometer - by Bioz Stars, 2019-12
    95/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: Following centrifugation, the RNA pellet was dissolved in 100 μL of RNAse-free water. .. All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide.

    Amplification:

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA). .. The viral RNA expression was examined using the real master mix (Tiangen Biotech Co. Ltd, Beijing, China).

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide. .. All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide.

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: Paragraph title: DNA isolation and PCR amplification ... DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).

    Synthesized:

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: Total RNA was isolated from pelleted cells and tumor tissue using the Qiagen RNeasy Mini Kit (74104, Hilden, Germany) according to the manufacturer’s protocol. .. The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol. .. PCR was performed in a 25 μl reaction mixture containing 2 μl of cDNA (from isolated RNA), 12.5 μl of the JumpStart REDTaq® Ready Mix (Sigma-Aldrich, P0982), 0.5 μl of 10 μM forward and reverse primers, and 9.5 μl of ddH2O.

    Construct:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively. .. RNA had a 280/260 ratio > 1.8, RNA integrity number (RIN) > 8 and total RNA > 1 μg, and was further processed for Illumina deep sequencing.

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    SYBR Green Assay:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: Total RNA was extracted from skeletal muscles using Trizol (Invitrogen) according to the manufacturer's instructions. .. RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume. .. PCR reactions were performed as follows: 8 min at 95 °C followed by 50 cycles: 15 s at 95 °C, 15 s at 60 °C and 15 s at 72 °C.

    Article Title: Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells
    Article Snippet: RNA was quantified using an ND-1000 NanoDrop Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, Delaware). .. Sample cDNA was produced using the iScript cDNA synthesis kit (Bio-Rad, 1708891).

    Incubation:

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: For DNA isolation, both mitochondrial and nuclear pellets were re-suspendedseparately in a lysis buffer with proteinase K and incubated at 37 ° C overnight. .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE).

    Article Title: A fully human CXCR4 antibody demonstrates diagnostic utility and therapeutic efficacy in solid tumor xenografts
    Article Snippet: To this solution a 5 fold excess of DFO in DMSO was added, ensuring less than 2% (v/v) DMSO in the final solution, followed by incubation at 37°C for 45 min. .. The final concentration of the mAb-DFO conjugate was determined using a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA).

    Infection:

    Article Title: Intensity of Mutualism Breakdown Is Determined by Temperature Not Amplification of Wolbachia Genes
    Article Snippet: Both Wolbachia infection density in adult flies and the copy number of the Octomom repeat locus in the Wolbachia genome were estimated using relative qPCR assays [ ]. .. Total DNA was estimated using an ND-1000 Nanodrop Spectrophotometer (Analytical Technologies, Collegeville, PA).

    Expressing:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: Paragraph title: Affymetrix mouse 2.0 ST arrays expression profiling ... RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) followed by Quality Control analysis (Agilent 2100 Bioanalyzer, Eukaryote Total RNA Nano assay, Agilent RNA 6000 Nano Kit, Agilent RNA Nano LabChip) to determine the RNA integrity number (RIN, 28S/18S ratio).

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume. .. RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume.

    Article Title: Connexin-43-dependent ATP release mediates macrophage activation during sepsis
    Article Snippet: Paragraph title: Gene expression analysis ... RNA concentration and quality were analysed by spectrophotometer NanoDrop ND-1000 (Thermo Scientific).

    Modification:

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    Western Blot:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Two adult female greater mouse-eared bats (Myotis myotis ) were captured in Béganne, western France (47°35′N, 2°14′W). .. The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively.

    Transformation Assay:

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    DNA Methylation Assay:

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: DNA for the analysis of DNA methylation was harvested and isolated using the FitAmpTM Blood & cultured Cell DNA Extraction Kit from EPIGENTEK® as previously described . .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE).

    Hybridization:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) followed by Quality Control analysis (Agilent 2100 Bioanalyzer, Eukaryote Total RNA Nano assay, Agilent RNA 6000 Nano Kit, Agilent RNA Nano LabChip) to determine the RNA integrity number (RIN, 28S/18S ratio). .. The sense strand ss cDNA was purified, fragmented and labelled using DNA-labelled reagent and then hybridized overnight onto the Mouse 2.0 ST arrays (100 or 81/4-Format).

    Transfection:

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: Paragraph title: Isolation of nuclear and mitochondrial DNA from transfected A549 and A549 DDP cells ... Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE).

    Cell Culture:

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: DNA for the analysis of DNA methylation was harvested and isolated using the FitAmpTM Blood & cultured Cell DNA Extraction Kit from EPIGENTEK® as previously described . .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE).

    Polymerase Chain Reaction:

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA). .. The viral RNA expression was examined using the real master mix (Tiangen Biotech Co. Ltd, Beijing, China).

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide. .. Quality cDNA control was performed by the amplification of an ACTB (NM 001101) fragment using forward (5´-CACTGTGTTG GCGTACAGGT-3′ and reverse primers (5´-TCATCACCATTGGCAATGAG-3′).

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: Paragraph title: Reverse transcription PCR ... The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol.

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume. .. PCR reactions were performed as follows: 8 min at 95 °C followed by 50 cycles: 15 s at 95 °C, 15 s at 60 °C and 15 s at 72 °C.

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: Paragraph title: DNA isolation and PCR amplification ... DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).

    Cellular Antioxidant Activity Assay:

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol. .. In total, 35 cycles were conducted with a final extension at 72 °C for 10 min. PCR products were electrophoresed on 2 % agarose gel and visualized under UV light.

    DNA Extraction:

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: DNA for the analysis of DNA methylation was harvested and isolated using the FitAmpTM Blood & cultured Cell DNA Extraction Kit from EPIGENTEK® as previously described . .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE).

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: Paragraph title: DNA isolation and PCR amplification ... DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).

    Real-time Polymerase Chain Reaction:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: Total RNA was extracted from skeletal muscles using Trizol (Invitrogen) according to the manufacturer's instructions. .. RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume. .. PCR reactions were performed as follows: 8 min at 95 °C followed by 50 cycles: 15 s at 95 °C, 15 s at 60 °C and 15 s at 72 °C.

    Article Title: Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells
    Article Snippet: Paragraph title: Quantitative Real-time PCR ... RNA was quantified using an ND-1000 NanoDrop Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, Delaware).

    Article Title: Design, optimization and validation of genes commonly used in expression studies on DMH/AOM rat colon carcinogenesis model
    Article Snippet: RNA quantity and purity (260/280 and 260/230 ratios) were assessed with a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was performed with the Maxima H Minus First Strand cDNA Synthesis kit with dsDNase (Ref. K1682; Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions (≤ 5 µg of total RNA as template and using 100 pmol random hexamer primer).

    Article Title: Intensity of Mutualism Breakdown Is Determined by Temperature Not Amplification of Wolbachia Genes
    Article Snippet: Both Wolbachia infection density in adult flies and the copy number of the Octomom repeat locus in the Wolbachia genome were estimated using relative qPCR assays [ ]. .. Total DNA was estimated using an ND-1000 Nanodrop Spectrophotometer (Analytical Technologies, Collegeville, PA).

    Mutagenesis:

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    Isolation:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Total RNA was isolated from the whole blood using RNAzol ® BD kit (catalog number RB192, Molecular Biology Center, Inc) according to the manufacturer’s instructions with minor modifications. .. The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively.

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: DNA for the analysis of DNA methylation was harvested and isolated using the FitAmpTM Blood & cultured Cell DNA Extraction Kit from EPIGENTEK® as previously described . .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE). .. The ELISA assays were conducted as described previously .

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: Total RNA was isolated from pelleted cells and tumor tissue using the Qiagen RNeasy Mini Kit (74104, Hilden, Germany) according to the manufacturer’s protocol. .. The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol.

    Article Title: Transcriptome changes in apple peel tissues during CO2 injury symptom development under controlled atmosphere storage regimens
    Article Snippet: Paragraph title: RNA isolation from apple fruit peel tissue ... The total RNA was quantified using an ND-1000 Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE), and the RNA quality was verified by agarose gel electrophoresis.

    Article Title: FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Article Snippet: Paragraph title: Isolation of RNA ... The concentration of RNA was determined using a ThermoFisher ND-1000 NanoDrop Spectrophotometer.

    Article Title: Connexin-43-dependent ATP release mediates macrophage activation during sepsis
    Article Snippet: In the liver, one cubic millimetre of the median lobe was used for RNA isolation. .. RNA concentration and quality were analysed by spectrophotometer NanoDrop ND-1000 (Thermo Scientific).

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: Genomic DNA was isolated from SLK and SLKK cells using the PureLink Genomic DNA Mini kit (Invitrogen) following the manufacturer’s instruction. .. DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. Eight individual regions of the 14q32 locus were probed using a set of custom-designed primers at 10μM ( ) and AmpliTaq Gold 360 Master Mix (Applied Biosystems).

    Article Title: Differential Targeting of c-Maf, Bach-1, and Elmo-1 by microRNA-143 and microRNA-365 Promotes the Intracellular Growth of Mycobacterium tuberculosis in Alternatively IL-4/IL-13 Activated Macrophages
    Article Snippet: Total RNA was isolated from the lysate using miRNeasy Mini kit (Qiagen, Germany) according to the manufacturer's instructions. .. RNA quantity and purity were measured using the ND-1000 NanoDrop spectrophotometer (ThermoScientific, DE, USA).

    Article Title: Design, optimization and validation of genes commonly used in expression studies on DMH/AOM rat colon carcinogenesis model
    Article Snippet: Paragraph title: RNA isolation & cDNA synthesis ... RNA quantity and purity (260/280 and 260/230 ratios) were assessed with a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Intensity of Mutualism Breakdown Is Determined by Temperature Not Amplification of Wolbachia Genes
    Article Snippet: Genomic DNA was isolated using a QIAGEN DNeasy Blood & Tissue Kit according to manufacturer instructions (QIAGEN, Doncaster, VIC). .. Total DNA was estimated using an ND-1000 Nanodrop Spectrophotometer (Analytical Technologies, Collegeville, PA).

    Article Title: Characterization of the general stress response in Bartonella henselae
    Article Snippet: Paragraph title: 2.5 RNA isolation ... The yield and quality of the extracted total RNA was evaluated by measuring the ultraviolet (UV) absorbance using the ND-1000 nanodrop spectrophotometer (Thermo Scientific, Wilmington, DE) and by agarose gel electrophoresis.

    RNA Extraction:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Paragraph title: Bat blood sampling, total RNA extraction and miRNA sequencing ... The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively.

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide.

    Article Title: Differential Targeting of c-Maf, Bach-1, and Elmo-1 by microRNA-143 and microRNA-365 Promotes the Intracellular Growth of Mycobacterium tuberculosis in Alternatively IL-4/IL-13 Activated Macrophages
    Article Snippet: Paragraph title: RNA Extraction ... RNA quantity and purity were measured using the ND-1000 NanoDrop spectrophotometer (ThermoScientific, DE, USA).

    Purification:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) followed by Quality Control analysis (Agilent 2100 Bioanalyzer, Eukaryote Total RNA Nano assay, Agilent RNA 6000 Nano Kit, Agilent RNA Nano LabChip) to determine the RNA integrity number (RIN, 28S/18S ratio). .. Ninety nanograms of total RNA per sample was further processed using the GeneChip WT PLUS Reagent Kit for whole transcript expression profiling (Affymetrix, Santa Clara, CA).

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    Article Title: FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Article Snippet: Ethanol (100%—1.5 volumes) was added and the RNA purified using a Qiagen miRNeasy mini kit according to the manufacturer's instructions. .. The concentration of RNA was determined using a ThermoFisher ND-1000 NanoDrop Spectrophotometer.

    Article Title: A fully human CXCR4 antibody demonstrates diagnostic utility and therapeutic efficacy in solid tumor xenografts
    Article Snippet: The resulting mAb-DFO was purified by size exclusion chromatography using Amicon Ultracel® 10K centrifugal filters (Merck Millipore Ltd., Tullagreen, Carrigtwohill, Co. Cork, IRL). .. The final concentration of the mAb-DFO conjugate was determined using a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA).

    Sequencing:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Paragraph title: Bat blood sampling, total RNA extraction and miRNA sequencing ... The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively.

    Quantitative RT-PCR:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: Paragraph title: qRT-PCR analysis ... RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume.

    Article Title: Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells
    Article Snippet: RNA was quantified using an ND-1000 NanoDrop Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, Delaware). .. Target mRNA was quantified using MyIQ single color real-time PCR detection system from Bio-Rad (Bio-Rad, Hercules, CA) and iQ SYBR green Supermix (Bio-Rad, 1708882).

    Staining:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) followed by Quality Control analysis (Agilent 2100 Bioanalyzer, Eukaryote Total RNA Nano assay, Agilent RNA 6000 Nano Kit, Agilent RNA Nano LabChip) to determine the RNA integrity number (RIN, 28S/18S ratio). .. Prior to hybridization, the arrays were registered on GeneChip Command Console Software (AGCC) (Affymetrix, Santa Clara, CA).

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: Following centrifugation, the RNA pellet was dissolved in 100 μL of RNAse-free water. .. All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide. .. Two micrograms of template RNA were used for first-strand cDNA synthesis using oligo (dT) primers and the reverse transcriptase Superscript III (Invitrogen, Carlsbad, CA) following manufacturer’s instructions.

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol. .. In total, 35 cycles were conducted with a final extension at 72 °C for 10 min. PCR products were electrophoresed on 2 % agarose gel and visualized under UV light.

    Mouse Assay:

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA). .. Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA).

    Plasmid Preparation:

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab).

    Software:

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) followed by Quality Control analysis (Agilent 2100 Bioanalyzer, Eukaryote Total RNA Nano assay, Agilent RNA 6000 Nano Kit, Agilent RNA Nano LabChip) to determine the RNA integrity number (RIN, 28S/18S ratio). .. The sense strand ss cDNA was purified, fragmented and labelled using DNA-labelled reagent and then hybridized overnight onto the Mouse 2.0 ST arrays (100 or 81/4-Format).

    Electrophoresis:

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: Following centrifugation, the RNA pellet was dissolved in 100 μL of RNAse-free water. .. All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide. .. Two micrograms of template RNA were used for first-strand cDNA synthesis using oligo (dT) primers and the reverse transcriptase Superscript III (Invitrogen, Carlsbad, CA) following manufacturer’s instructions.

    Virus Quantification:

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Paragraph title: 2.5. Lung Index and Virus Quantification ... Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA).

    RNA Expression:

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA). .. The RNA of each sample was diluted to the same concentration and was reverse-transcribed to complementary DNA (cDNA) using the Reverse Transcription Kit (Tiangen, Biotech Co. Ltd, Beijing, China) according to the manufacturer’s protocol.

    Agarose Gel Electrophoresis:

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: Following centrifugation, the RNA pellet was dissolved in 100 μL of RNAse-free water. .. All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide. .. Two micrograms of template RNA were used for first-strand cDNA synthesis using oligo (dT) primers and the reverse transcriptase Superscript III (Invitrogen, Carlsbad, CA) following manufacturer’s instructions.

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol. .. The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol.

    Article Title: Transcriptome changes in apple peel tissues during CO2 injury symptom development under controlled atmosphere storage regimens
    Article Snippet: Total RNAs from three biological replicates were pooled to represent the sample per treatment and storage duration. .. The total RNA was quantified using an ND-1000 Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE), and the RNA quality was verified by agarose gel electrophoresis. .. The quantity and quality of isolated peel RNA was examined using a Nanodrop ND-1000 (Thermo Scientific, Waltham, MA) and 2100 bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Characterization of the general stress response in Bartonella henselae
    Article Snippet: The RNA was subsequently treated with DNase using the Turbo DNA-free kit (Thermo Fisher Scientific, Grand Island, NY) according to the manufacturer’s protocol to remove any contaminating DNA. .. The yield and quality of the extracted total RNA was evaluated by measuring the ultraviolet (UV) absorbance using the ND-1000 nanodrop spectrophotometer (Thermo Scientific, Wilmington, DE) and by agarose gel electrophoresis. .. The DNase-treated RNA (1µg) was reverse transcribed into cDNA in a 20-µl reaction using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA).

    Size-exclusion Chromatography:

    Article Title: A fully human CXCR4 antibody demonstrates diagnostic utility and therapeutic efficacy in solid tumor xenografts
    Article Snippet: The resulting mAb-DFO was purified by size exclusion chromatography using Amicon Ultracel® 10K centrifugal filters (Merck Millipore Ltd., Tullagreen, Carrigtwohill, Co. Cork, IRL). .. The final concentration of the mAb-DFO conjugate was determined using a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA).

    Random Hexamer Labeling:

    Article Title: Design, optimization and validation of genes commonly used in expression studies on DMH/AOM rat colon carcinogenesis model
    Article Snippet: RNA quantity and purity (260/280 and 260/230 ratios) were assessed with a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Furthermore, the integrity of the total RNA obtained was evaluated through 1% agarose gel ( ).

    Spectrophotometry:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Bat sampling, blood collection and total RNA extraction were carried out as described in our previous study [ ]. .. The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively. .. RNA had a 280/260 ratio > 1.8, RNA integrity number (RIN) > 8 and total RNA > 1 μg, and was further processed for Illumina deep sequencing.

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Approximately 20 mg of lung tissue was separated from the lung, and the total RNA was extracted with 1 mL of Beyozol reagent (Beyotime Biotechnology, Haimen, China). .. Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA). .. The purity of RNA samples was assessed by measuring the optical density ratio at 260 and 280 nm (OD260/OD280).

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: Quality control was performed on total RNA extracted from mouse muscles using a Nanodrop and Bioanalyzer (Agilent Technologies, Santa Clara, CA). .. RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) followed by Quality Control analysis (Agilent 2100 Bioanalyzer, Eukaryote Total RNA Nano assay, Agilent RNA 6000 Nano Kit, Agilent RNA Nano LabChip) to determine the RNA integrity number (RIN, 28S/18S ratio). .. Ninety nanograms of total RNA per sample was further processed using the GeneChip WT PLUS Reagent Kit for whole transcript expression profiling (Affymetrix, Santa Clara, CA).

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: DNA for the analysis of DNA methylation was harvested and isolated using the FitAmpTM Blood & cultured Cell DNA Extraction Kit from EPIGENTEK® as previously described . .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE). .. The ELISA assays were conducted as described previously .

    Article Title: In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer
    Article Snippet: Following centrifugation, the RNA pellet was dissolved in 100 μL of RNAse-free water. .. All extracted RNA samples were quantified in the spectrophotometer NanoDrop-ND 1000 (Thermo Scientific, Wilmington, DE) and analyzed by electrophoresis in 1% agarose gel stained by 0.5 μg/mL ethidium bromide. .. Two micrograms of template RNA were used for first-strand cDNA synthesis using oligo (dT) primers and the reverse transcriptase Superscript III (Invitrogen, Carlsbad, CA) following manufacturer’s instructions.

    Article Title: Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG
    Article Snippet: The plasmid expressing D637L mutant of the RNA edited (containing 8As at editing site) GP gene of Zaire EBOV strain Mayinga under the control of a modified human cytomegalovirus promoter was constructed from phCMVGP by mutating amino acid codon 637 in EBOV GP from D to L. The plasmid DNA for immunizations was purified from transformed E. coli DH5α strain using EndoFree Plasmid Giga Kit (Qiagen). .. DNA concentrations were determined using ND-1000 NanoDrop Spectrophotometer (Biolab). .. KUN-VLP-GP/D637L were prepared as described previously and concentrated by centrifugation through Amicon Ultra centrifugal filter devices, with a 100,000 molecular weight cut off (Millipore, Billerica, Massachusetts).

    Article Title: The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
    Article Snippet: Total RNA was isolated from pelleted cells and tumor tissue using the Qiagen RNeasy Mini Kit (74104, Hilden, Germany) according to the manufacturer’s protocol. .. The quantity and quality of the extracted RNA was determined with the use of a spectrophotometer NanoDrop ND-1000 (Wilmington, DE, USA). cDNA was synthesized from 1 μg of total RNA using the QuantiTect Reverse Transcription Kit (205310, Hilden) according to the manufacturer’s protocol. .. PCR was performed in a 25 μl reaction mixture containing 2 μl of cDNA (from isolated RNA), 12.5 μl of the JumpStart REDTaq® Ready Mix (Sigma-Aldrich, P0982), 0.5 μl of 10 μM forward and reverse primers, and 9.5 μl of ddH2O.

    Article Title: Transcriptome changes in apple peel tissues during CO2 injury symptom development under controlled atmosphere storage regimens
    Article Snippet: Total RNAs from three biological replicates were pooled to represent the sample per treatment and storage duration. .. The total RNA was quantified using an ND-1000 Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE), and the RNA quality was verified by agarose gel electrophoresis. .. The quantity and quality of isolated peel RNA was examined using a Nanodrop ND-1000 (Thermo Scientific, Waltham, MA) and 2100 bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).

    Article Title: PABPN1 gene therapy for oculopharyngeal muscular dystrophy
    Article Snippet: Total RNA was extracted from skeletal muscles using Trizol (Invitrogen) according to the manufacturer's instructions. .. RNA quality and purity was determined using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and RNA (50–250 ng for muscle biopsies, 1–3 μg for cell pellet) was reverse transcribed using M-MLV reverse transcriptase (Invitrogen) according to the manufacturer's instructions. cDNA was used for qPCR reaction using SYBR green mix buffer (LightCycler 480 Sybr green I Master) in a total of 9 μl reaction volume. .. PCR reactions were performed as follows: 8 min at 95 °C followed by 50 cycles: 15 s at 95 °C, 15 s at 60 °C and 15 s at 72 °C.

    Article Title: FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Article Snippet: The optional DNase step, the optional drying of the column, and back-elution were all included. .. The concentration of RNA was determined using a ThermoFisher ND-1000 NanoDrop Spectrophotometer. .. The quality of the RNA was determined using an Agilent Bioanalyzer according to the manufacturer's instructions.

    Article Title: A fully human CXCR4 antibody demonstrates diagnostic utility and therapeutic efficacy in solid tumor xenografts
    Article Snippet: The resulting mAb-DFO was purified by size exclusion chromatography using Amicon Ultracel® 10K centrifugal filters (Merck Millipore Ltd., Tullagreen, Carrigtwohill, Co. Cork, IRL). .. The final concentration of the mAb-DFO conjugate was determined using a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. In a typical reaction, 89 Zr-oxalate was neutralized with 2M Na2 CO3 to pH 7.

    Article Title: Connexin-43-dependent ATP release mediates macrophage activation during sepsis
    Article Snippet: In the liver, one cubic millimetre of the median lobe was used for RNA isolation. .. RNA concentration and quality were analysed by spectrophotometer NanoDrop ND-1000 (Thermo Scientific). .. 500 ng of total RNA was used for cDNA synthesis. cDNA was synthesised by using Omniscript RT Kit 200 (Quiagen, #205113). qPCR analysis was performed by using TaqMan gene expression assays (Applied Biosystem) according to manufacturer’s protocol. mRNA was analysed by reverse transcriptase-quantitative PCR (ABI 7900, SDS 2.3 software).

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: Genomic DNA was isolated from SLK and SLKK cells using the PureLink Genomic DNA Mini kit (Invitrogen) following the manufacturer’s instruction. .. DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. Eight individual regions of the 14q32 locus were probed using a set of custom-designed primers at 10μM ( ) and AmpliTaq Gold 360 Master Mix (Applied Biosystems).

    Article Title: Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells
    Article Snippet: Total RNA was extracted from cells using the MiRNeasy Mini Kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany, 217004). .. RNA was quantified using an ND-1000 NanoDrop Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, Delaware). .. The 260/280 ratios were routinely over 1.9.

    Article Title: Differential Targeting of c-Maf, Bach-1, and Elmo-1 by microRNA-143 and microRNA-365 Promotes the Intracellular Growth of Mycobacterium tuberculosis in Alternatively IL-4/IL-13 Activated Macrophages
    Article Snippet: Total RNA was isolated from the lysate using miRNeasy Mini kit (Qiagen, Germany) according to the manufacturer's instructions. .. RNA quantity and purity were measured using the ND-1000 NanoDrop spectrophotometer (ThermoScientific, DE, USA). .. For protein coding gene expression analysis, 100 ng total RNA was reverse-transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany) according to the manufacturer's instructions.

    Article Title: Design, optimization and validation of genes commonly used in expression studies on DMH/AOM rat colon carcinogenesis model
    Article Snippet: Total RNA was extracted using the Trizol™ Plus PureLink™ Kit RNA Mini Kit (Invitrogen, USA) following the kit instructions. .. RNA quantity and purity (260/280 and 260/230 ratios) were assessed with a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Furthermore, the integrity of the total RNA obtained was evaluated through 1% agarose gel ( ).

    Article Title: Ag85A DNA Vaccine Delivery by Nanoparticles: Influence of the Formulation Characteristics on Immune Responses
    Article Snippet: The loading capacity of pDNA at different concentrations with a fixed amount of TMC nanoparticles was quantified by the separation of the nanoparticles from unloaded pDNA using centrifugal ultrafiltration (Vivaspin, Sartorius, Göttingen, Germany). .. Samples were centrifuged through 300-kDa polyethersulfone (PES) membranes at 3220× g for 20 min, and non-adsorbed pDNA in the filtrate was determined by measuring the absorption at a wavelength of 260 nm with an ND-1000 NanoDrop spectrophotometer (Thermo Scientific, Illkirch-Graffenstaden, France). .. Loading efficiency was expressed as a percentage as the amount of adsorbed pDNA compared to the amount of pDNA initially used to form the nanocomplexes.

    Article Title: Intensity of Mutualism Breakdown Is Determined by Temperature Not Amplification of Wolbachia Genes
    Article Snippet: Genomic DNA was isolated using a QIAGEN DNeasy Blood & Tissue Kit according to manufacturer instructions (QIAGEN, Doncaster, VIC). .. Total DNA was estimated using an ND-1000 Nanodrop Spectrophotometer (Analytical Technologies, Collegeville, PA). .. To estimate the relative abundance of Wolbachia bacteria in each sample, we compared the abundance of the single-copy Wolbachia ankyrin repeat gene WD0550 to that of the single-copy D . melanogaster gene Act88F [ ].

    Article Title: Characterization of the general stress response in Bartonella henselae
    Article Snippet: The RNA was subsequently treated with DNase using the Turbo DNA-free kit (Thermo Fisher Scientific, Grand Island, NY) according to the manufacturer’s protocol to remove any contaminating DNA. .. The yield and quality of the extracted total RNA was evaluated by measuring the ultraviolet (UV) absorbance using the ND-1000 nanodrop spectrophotometer (Thermo Scientific, Wilmington, DE) and by agarose gel electrophoresis. .. The DNase-treated RNA (1µg) was reverse transcribed into cDNA in a 20-µl reaction using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA).

    Sampling:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Paragraph title: Bat blood sampling, total RNA extraction and miRNA sequencing ... The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively.

    Produced:

    Article Title: Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells
    Article Snippet: RNA was quantified using an ND-1000 NanoDrop Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, Delaware). .. RNA was quantified using an ND-1000 NanoDrop Spectrophotometer (NanoDrop Technologies, Inc., Wilmington, Delaware).

    Concentration Assay:

    Article Title: Blood miRNomes and transcriptomes reveal novel longevity mechanisms in the long-lived bat, Myotis myotis
    Article Snippet: Bat sampling, blood collection and total RNA extraction were carried out as described in our previous study [ ]. .. The concentration and quality of total RNA were quantified and evaluated for each sample by a Nanodrop-ND-1000 Spectrophotometer (Thermo Scientific) and a 2100 Bioanalyzer (Aligent Technologies), respectively. .. RNA had a 280/260 ratio > 1.8, RNA integrity number (RIN) > 8 and total RNA > 1 μg, and was further processed for Illumina deep sequencing.

    Article Title: Alteration of Intestinal Flora Stimulates Pulmonary microRNAs to Interfere with Host Antiviral Immunity in Influenza
    Article Snippet: Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA). .. Nucleic acid concentrations were quantified with a Thermo Nanodrop ND-1000 spectrophotometer (Rockland, DE, USA).

    Article Title: FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Article Snippet: The optional DNase step, the optional drying of the column, and back-elution were all included. .. The concentration of RNA was determined using a ThermoFisher ND-1000 NanoDrop Spectrophotometer. .. The quality of the RNA was determined using an Agilent Bioanalyzer according to the manufacturer's instructions.

    Article Title: A fully human CXCR4 antibody demonstrates diagnostic utility and therapeutic efficacy in solid tumor xenografts
    Article Snippet: The resulting mAb-DFO was purified by size exclusion chromatography using Amicon Ultracel® 10K centrifugal filters (Merck Millipore Ltd., Tullagreen, Carrigtwohill, Co. Cork, IRL). .. The final concentration of the mAb-DFO conjugate was determined using a ND-1000 Nanodrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. In a typical reaction, 89 Zr-oxalate was neutralized with 2M Na2 CO3 to pH 7.

    Article Title: Connexin-43-dependent ATP release mediates macrophage activation during sepsis
    Article Snippet: In the liver, one cubic millimetre of the median lobe was used for RNA isolation. .. RNA concentration and quality were analysed by spectrophotometer NanoDrop ND-1000 (Thermo Scientific). .. 500 ng of total RNA was used for cDNA synthesis. cDNA was synthesised by using Omniscript RT Kit 200 (Quiagen, #205113). qPCR analysis was performed by using TaqMan gene expression assays (Applied Biosystem) according to manufacturer’s protocol. mRNA was analysed by reverse transcriptase-quantitative PCR (ABI 7900, SDS 2.3 software).

    Article Title: Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells
    Article Snippet: Genomic DNA was isolated from SLK and SLKK cells using the PureLink Genomic DNA Mini kit (Invitrogen) following the manufacturer’s instruction. .. DNA concentration and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. Eight individual regions of the 14q32 locus were probed using a set of custom-designed primers at 10μM ( ) and AmpliTaq Gold 360 Master Mix (Applied Biosystems).

    Lysis:

    Article Title: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells
    Article Snippet: For DNA isolation, both mitochondrial and nuclear pellets were re-suspendedseparately in a lysis buffer with proteinase K and incubated at 37 ° C overnight. .. Isolated DNA was quantified using a ND-1000 NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE).

    Hood:

    Article Title: Transcriptome changes in apple peel tissues during CO2 injury symptom development under controlled atmosphere storage regimens
    Article Snippet: The supernatant was discarded, and tubes were placed in the hood inverted on Kimwipes for 3–5 min and air dried for 15–20 min. Total RNA pellets were dissolved in 30 µL of molecular biology–grade water (Sigma-Aldrich). .. The total RNA was quantified using an ND-1000 Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE), and the RNA quality was verified by agarose gel electrophoresis.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher 2000c spectrophotometer
    2000c Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2000c spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 1330 article reviews
    Price from $9.99 to $1999.99
    2000c spectrophotometer - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher nanodrop nd 1000 spectrophotometer
    Nanodrop Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop nd 1000 spectrophotometer/product/Thermo Fisher
    Average 95 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    nanodrop nd 1000 spectrophotometer - by Bioz Stars, 2019-12
    95/100 stars
      Buy from Supplier

    85
    Thermo Fisher nano drop instrument
    Nano Drop Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop instrument/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nano drop instrument - by Bioz Stars, 2019-12
    85/100 stars
      Buy from Supplier

    82
    Thermo Fisher nano drop 1000 spectrophotometry
    Nano Drop 1000 Spectrophotometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano drop 1000 spectrophotometry/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano drop 1000 spectrophotometry - by Bioz Stars, 2019-12
    82/100 stars
      Buy from Supplier

    Image Search Results