nanodrop 1000  (Thermo Fisher)


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    Name:
    NanoDrop One Spectrophotometer
    Description:
    For DNA RNA or protein samples that are 100 pure the results obtained on the Thermo Scientific NanoDrop One Microvolume UV Vis Spectrophotometer and the Invitrogen Qubit 4 Fluorometer would be identical for samples within their dynamic ranges However few samples are 100 pure As a bundled offering the NanoDrop One Microvolume UV Vis Spectrophotometer and the Qubit 4 Fluorometer together allow you to obtain the most complete information about the concentration and quality of your DNA RNA or protein samples helping to prevent costly troubleshooting downstream While both the NanoDrop One spectrophotometer and the Qubit 4 fluorometer offer accurate measurements of DNA RNA and protein samples each also has its unique advantages NanoDrop One Spectrophotometer Cited in more than 30 000 publications the NanoDrop UV Vis microvolume spectrophotometers have fundamentally changed the way scientists evaluate nucleic acid and protein samples by minimizing the need for large sample volumes The next generation NanoDrop One Microvolume UV Vis Spectrophotometer utilizes innovative Thermo Scientific Acclaro Sample Intelligence technology that identifies contaminants provides corrected concentration values and helps you understand the quality of your sample before you use it in downstream applications Qubit 4 Fluorometer The Qubit 4 Fluorometer is a cutting edge benchtop device that accurately measures DNA RNA and protein quantity and now also RNA integrity and quality using the highly sensitive Qubit assays For all Qubit assays the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target which minimizes the effects of contaminants on the result The easy to use touchscreen menus make it easy to select and run the assays you need with results displayed in just a few seconds Comparison of the NanoDrop One spectrophotometer and Qubit 4 Fluorometer NanoDrop One Spectrophotometer Qubit 4 Fluorometer Fast and simple Allows for direct measurement of purified samples with no dilutions required Overview Sensitive and specific Accurately measures concentration whether dsDNA ssDNA total RNA microRNA or protein even in the presence of contaminants UV absorbance Technology Fluorescence Pipette sample onto pedestal measure Workflow Mix sample with reagents incubate for 2 5 minutes read Routine quantitation and qualification of DNA RNA and proteins Applications Routine samples and with very dilute or difficult to process samples when higher measurement sensitivity is required or when nucleic acid contaminants are suspected e g DNA in RNA dNTPs RNA sample quality 0 21 27 500 dsDNA quantitation range ng ul 0 01 1 000 Acclaro Sample Intelligence technology identifies presence of contaminants such as protein or guanidine provides corrected analyte concentration value Sample qualification purity Unique fluorescent dyes enable differentiation between nucleic acid types e g DNA vs RNA without any sample pre treatment RNA IQ assay enables fast and facile detection of intact vs degraded in 5 seconds per sample Selection of pre programmed applications Direct A280 quantitation with Protein Editor A205 peptide bond absorbance Colorimetric protein assays Bradford BCA Lowry and Thermo Scientific Pierce 660 nm assay Protein Quantitation Qubit assays exhibit minimal protein to protein variability and are resistant to most contaminants including salts reducing agents e g DTT b mercaoptoethanol DNA or amino acids but not detergents 1dsDNA quantitation range for NanoDrop One is 0 2 ng µL with cuvette and 2 0 ng µL without Key instrument features include NanoDrop One Microvolume UV Vis Spectrophotometer • Fast and easy sample evaluation of nucleic acids and proteins with “pipette measure clean workflow and no sample carryover • Modern stand alone design with local control and high resolution touchscreen interface which saves bench space • Accurate measurements up to 27 500 ng µL dsDNA with extended auto range path length technology • No need for cuvettes or consumables needed so you can pipette directly onto pedestal sample retention system • Pre programmed applications designed specifically for life scientists • Enhanced connectivity with data transfer via USB Ethernet Bluetooth and WiFi options PC software available for data management Qubit 4 Fluorometer • Fast and highly accurate quantitation of DNA RNA and protein in less than three seconds per sample • Measures intact RNA in less than 5 seconds per sample • High levels of accuracy using only 1 20 μL of sample even with very dilute samples • On board Reagent Calculator quickly generates Qubit working solution preparation instructions • Stores results from up to 1000 samples • Large 5 7 inch state of the art color touchscreen for easy workflow navigation • Graphical display indicates when samples are in the extended range or out of range • Small footprint saves space on your bench • Exports data to a USB drive or directly to your computer via a USB cable • Ability to personalize your Qubit fluorometer with the assays you run most add new assays or even create your own assays with the MyQubit software and web tool • Language of your choice including English French Spanish Italian German simplified Chinese and Japanese
    Catalog Number:
    a38189
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Quantitation|Nucleic Acid Quantitation
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher nanodrop 1000
    For DNA RNA or protein samples that are 100 pure the results obtained on the Thermo Scientific NanoDrop One Microvolume UV Vis Spectrophotometer and the Invitrogen Qubit 4 Fluorometer would be identical for samples within their dynamic ranges However few samples are 100 pure As a bundled offering the NanoDrop One Microvolume UV Vis Spectrophotometer and the Qubit 4 Fluorometer together allow you to obtain the most complete information about the concentration and quality of your DNA RNA or protein samples helping to prevent costly troubleshooting downstream While both the NanoDrop One spectrophotometer and the Qubit 4 fluorometer offer accurate measurements of DNA RNA and protein samples each also has its unique advantages NanoDrop One Spectrophotometer Cited in more than 30 000 publications the NanoDrop UV Vis microvolume spectrophotometers have fundamentally changed the way scientists evaluate nucleic acid and protein samples by minimizing the need for large sample volumes The next generation NanoDrop One Microvolume UV Vis Spectrophotometer utilizes innovative Thermo Scientific Acclaro Sample Intelligence technology that identifies contaminants provides corrected concentration values and helps you understand the quality of your sample before you use it in downstream applications Qubit 4 Fluorometer The Qubit 4 Fluorometer is a cutting edge benchtop device that accurately measures DNA RNA and protein quantity and now also RNA integrity and quality using the highly sensitive Qubit assays For all Qubit assays the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target which minimizes the effects of contaminants on the result The easy to use touchscreen menus make it easy to select and run the assays you need with results displayed in just a few seconds Comparison of the NanoDrop One spectrophotometer and Qubit 4 Fluorometer NanoDrop One Spectrophotometer Qubit 4 Fluorometer Fast and simple Allows for direct measurement of purified samples with no dilutions required Overview Sensitive and specific Accurately measures concentration whether dsDNA ssDNA total RNA microRNA or protein even in the presence of contaminants UV absorbance Technology Fluorescence Pipette sample onto pedestal measure Workflow Mix sample with reagents incubate for 2 5 minutes read Routine quantitation and qualification of DNA RNA and proteins Applications Routine samples and with very dilute or difficult to process samples when higher measurement sensitivity is required or when nucleic acid contaminants are suspected e g DNA in RNA dNTPs RNA sample quality 0 21 27 500 dsDNA quantitation range ng ul 0 01 1 000 Acclaro Sample Intelligence technology identifies presence of contaminants such as protein or guanidine provides corrected analyte concentration value Sample qualification purity Unique fluorescent dyes enable differentiation between nucleic acid types e g DNA vs RNA without any sample pre treatment RNA IQ assay enables fast and facile detection of intact vs degraded in 5 seconds per sample Selection of pre programmed applications Direct A280 quantitation with Protein Editor A205 peptide bond absorbance Colorimetric protein assays Bradford BCA Lowry and Thermo Scientific Pierce 660 nm assay Protein Quantitation Qubit assays exhibit minimal protein to protein variability and are resistant to most contaminants including salts reducing agents e g DTT b mercaoptoethanol DNA or amino acids but not detergents 1dsDNA quantitation range for NanoDrop One is 0 2 ng µL with cuvette and 2 0 ng µL without Key instrument features include NanoDrop One Microvolume UV Vis Spectrophotometer • Fast and easy sample evaluation of nucleic acids and proteins with “pipette measure clean workflow and no sample carryover • Modern stand alone design with local control and high resolution touchscreen interface which saves bench space • Accurate measurements up to 27 500 ng µL dsDNA with extended auto range path length technology • No need for cuvettes or consumables needed so you can pipette directly onto pedestal sample retention system • Pre programmed applications designed specifically for life scientists • Enhanced connectivity with data transfer via USB Ethernet Bluetooth and WiFi options PC software available for data management Qubit 4 Fluorometer • Fast and highly accurate quantitation of DNA RNA and protein in less than three seconds per sample • Measures intact RNA in less than 5 seconds per sample • High levels of accuracy using only 1 20 μL of sample even with very dilute samples • On board Reagent Calculator quickly generates Qubit working solution preparation instructions • Stores results from up to 1000 samples • Large 5 7 inch state of the art color touchscreen for easy workflow navigation • Graphical display indicates when samples are in the extended range or out of range • Small footprint saves space on your bench • Exports data to a USB drive or directly to your computer via a USB cable • Ability to personalize your Qubit fluorometer with the assays you run most add new assays or even create your own assays with the MyQubit software and web tool • Language of your choice including English French Spanish Italian German simplified Chinese and Japanese
    https://www.bioz.com/result/nanodrop 1000/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    nanodrop 1000 - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular subtyping of nasopharyngeal carcinoma (NPC) and a microRNA-based prognostic model for distant metastasis
    Article Snippet: .. RNA concentration was determined by NanoDrop One spectrophotometer (Thermo Fisher Scientific, USA). cDNA was reverse transcribed from 1 μg of total RNA using miScript II RT Kit (QIAGEN, USA). qPCR was carried out with miScript SYBR Green PCR Kit (QIAGEN, USA) on the LightCycler 480 System (Roche, Switzerland). hsa-miR-26a, hsa-miR-29b, hsa-miR-200b, hsa-miR-370, hsa-miR-622, hsa-miR-1248, hsa-miR-1293, hsa-miR-2053, hsa-let-7d and hsa-let-7 were predesigned primers (miScript Primer Assays MS00029239, MS00009289, MS00009023, MS00045885, MS00005117, MS00014238, MS00014539, MS00044569, MS00003136 and MS00006489, QIAGEN, USA). .. Amplification reactions were done in triplicate for each examined sample.

    Article Title: Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants
    Article Snippet: .. Briefly, total RNA from tracheal graft was extracted using RNeasy mini kit 50 (Qiagen Sciences, Maryland, USA.) and quantified using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies, USA). cDNA from each isolated RNA was synthesized with a Superscript™ II cDNA reverse transcription kit (ThermoFisher Scientific) and real time-PCR was performed using gene-specific primers on an AB 7500 Fast Real-Time PCR system in triplicates using Power SYBR Green (ThermoFisher Scientific). .. Data were analyzed with integrated software, and expression levels were analyzed by the 2−∆∆Ct method after normalization to the housekeeping genes glutaraldehyde dehydrogenase (GAPDH).

    Agarose Gel Electrophoresis:

    Article Title: The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination
    Article Snippet: .. For Southern blot analyses, genomic DNA from the wild type and two transformants was extracted from filtered samples following Weinkove et al. , and digested with Nco I and Bcl I. DNA was quantified using a Nanodrop ND-100 spectrophotometer (Nanodrop Technologies), and the same amounts of digested DNA were loaded per lane on a 0.8% agarose gel. .. After electrophoresis, DNA fragmentes were transferred to a Hybond-N membrane (GE Healthcare).

    Southern Blot:

    Article Title: The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination
    Article Snippet: .. For Southern blot analyses, genomic DNA from the wild type and two transformants was extracted from filtered samples following Weinkove et al. , and digested with Nco I and Bcl I. DNA was quantified using a Nanodrop ND-100 spectrophotometer (Nanodrop Technologies), and the same amounts of digested DNA were loaded per lane on a 0.8% agarose gel. .. After electrophoresis, DNA fragmentes were transferred to a Hybond-N membrane (GE Healthcare).

    Synthesized:

    Article Title: Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants
    Article Snippet: .. Briefly, total RNA from tracheal graft was extracted using RNeasy mini kit 50 (Qiagen Sciences, Maryland, USA.) and quantified using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies, USA). cDNA from each isolated RNA was synthesized with a Superscript™ II cDNA reverse transcription kit (ThermoFisher Scientific) and real time-PCR was performed using gene-specific primers on an AB 7500 Fast Real-Time PCR system in triplicates using Power SYBR Green (ThermoFisher Scientific). .. Data were analyzed with integrated software, and expression levels were analyzed by the 2−∆∆Ct method after normalization to the housekeeping genes glutaraldehyde dehydrogenase (GAPDH).

    Isolation:

    Article Title: Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants
    Article Snippet: .. Briefly, total RNA from tracheal graft was extracted using RNeasy mini kit 50 (Qiagen Sciences, Maryland, USA.) and quantified using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies, USA). cDNA from each isolated RNA was synthesized with a Superscript™ II cDNA reverse transcription kit (ThermoFisher Scientific) and real time-PCR was performed using gene-specific primers on an AB 7500 Fast Real-Time PCR system in triplicates using Power SYBR Green (ThermoFisher Scientific). .. Data were analyzed with integrated software, and expression levels were analyzed by the 2−∆∆Ct method after normalization to the housekeeping genes glutaraldehyde dehydrogenase (GAPDH).

    Spectrophotometry:

    Article Title: Isolation and identification of a novel protein elicitor from a Bacillus subtilis strain BU412
    Article Snippet: .. All protein concentrations were measured using NanoDrop One UV Spectrophotometer (Thermo Scientific). .. HR and Trypan blue staining In order to check the effect of the new protein elicitor for the HR-inducing activity in tobacco, 1 mg mL−1 protein elicitor was infiltrated into the leaves using a syringe without needle to cover areas of 1 cm2 .

    Article Title: Molecular subtyping of nasopharyngeal carcinoma (NPC) and a microRNA-based prognostic model for distant metastasis
    Article Snippet: .. RNA concentration was determined by NanoDrop One spectrophotometer (Thermo Fisher Scientific, USA). cDNA was reverse transcribed from 1 μg of total RNA using miScript II RT Kit (QIAGEN, USA). qPCR was carried out with miScript SYBR Green PCR Kit (QIAGEN, USA) on the LightCycler 480 System (Roche, Switzerland). hsa-miR-26a, hsa-miR-29b, hsa-miR-200b, hsa-miR-370, hsa-miR-622, hsa-miR-1248, hsa-miR-1293, hsa-miR-2053, hsa-let-7d and hsa-let-7 were predesigned primers (miScript Primer Assays MS00029239, MS00009289, MS00009023, MS00045885, MS00005117, MS00014238, MS00014539, MS00044569, MS00003136 and MS00006489, QIAGEN, USA). .. Amplification reactions were done in triplicate for each examined sample.

    Article Title: Functional and mechanistic advantage of the use of a bifunctional anti-PD-L1/IL-15 superagonist
    Article Snippet: .. RNA purity was assessed on the Nanodrop One Spectrophotometer (Thermo Fisher Scientific) and Agilent Bioanalyzer (Agilent). .. RNA analysis was performed using the PanCancer Mouse IO 360 Panel and data analyzed using the nSolver Software and nCounter Advanced Analysis Software (NanoString).

    Article Title: Investigation of candidate genes involved in the rhodoquinone biosynthetic pathway in Rhodospirillum rubrum
    Article Snippet: .. The concentration and quality of RNA (A260/280 ) was determined using a Nanodrop One UV-Vis spectrophotometer (Thermofisher Scientific, Waltham, MA). .. PCR with the Rru_A3231 in-gene primers ( 5’- TTCTGACCTTGCTGGCGATC-3’ and 3’- GGCTGATGCTGGCATCAAG-5’ ) was performed on each sample in order to test for genomic DNA contamination.

    Article Title: Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants
    Article Snippet: .. Briefly, total RNA from tracheal graft was extracted using RNeasy mini kit 50 (Qiagen Sciences, Maryland, USA.) and quantified using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies, USA). cDNA from each isolated RNA was synthesized with a Superscript™ II cDNA reverse transcription kit (ThermoFisher Scientific) and real time-PCR was performed using gene-specific primers on an AB 7500 Fast Real-Time PCR system in triplicates using Power SYBR Green (ThermoFisher Scientific). .. Data were analyzed with integrated software, and expression levels were analyzed by the 2−∆∆Ct method after normalization to the housekeeping genes glutaraldehyde dehydrogenase (GAPDH).

    Article Title: Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling
    Article Snippet: .. The concentration and purity of RNA were measured with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific), and RNA was kept at − 80 °C. .. Residual DNAs in total RNAs were removed and cDNAs were synthesized by Hifair II 1st Strand cDNA Synthesis SuperMix (11123ES60, YEASEN, Shanghai, China) following the manufacturer’s protocol.

    Article Title: Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632
    Article Snippet: .. RNA purity and concentration was assessed using the Nanodrop One UV-Vis Spectrophotometer (ThermoFischer Scientific, Waltham, MA). .. Library preparation was completed using the Illumina Neoprep Library Preparation System (Illumina, San Diego, CA).

    Article Title: The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination
    Article Snippet: .. For Southern blot analyses, genomic DNA from the wild type and two transformants was extracted from filtered samples following Weinkove et al. , and digested with Nco I and Bcl I. DNA was quantified using a Nanodrop ND-100 spectrophotometer (Nanodrop Technologies), and the same amounts of digested DNA were loaded per lane on a 0.8% agarose gel. .. After electrophoresis, DNA fragmentes were transferred to a Hybond-N membrane (GE Healthcare).

    SYBR Green Assay:

    Article Title: Molecular subtyping of nasopharyngeal carcinoma (NPC) and a microRNA-based prognostic model for distant metastasis
    Article Snippet: .. RNA concentration was determined by NanoDrop One spectrophotometer (Thermo Fisher Scientific, USA). cDNA was reverse transcribed from 1 μg of total RNA using miScript II RT Kit (QIAGEN, USA). qPCR was carried out with miScript SYBR Green PCR Kit (QIAGEN, USA) on the LightCycler 480 System (Roche, Switzerland). hsa-miR-26a, hsa-miR-29b, hsa-miR-200b, hsa-miR-370, hsa-miR-622, hsa-miR-1248, hsa-miR-1293, hsa-miR-2053, hsa-let-7d and hsa-let-7 were predesigned primers (miScript Primer Assays MS00029239, MS00009289, MS00009023, MS00045885, MS00005117, MS00014238, MS00014539, MS00044569, MS00003136 and MS00006489, QIAGEN, USA). .. Amplification reactions were done in triplicate for each examined sample.

    Article Title: Hypoxia-induced complement dysregulation is associated with microvascular impairments in mouse tracheal transplants
    Article Snippet: .. Briefly, total RNA from tracheal graft was extracted using RNeasy mini kit 50 (Qiagen Sciences, Maryland, USA.) and quantified using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies, USA). cDNA from each isolated RNA was synthesized with a Superscript™ II cDNA reverse transcription kit (ThermoFisher Scientific) and real time-PCR was performed using gene-specific primers on an AB 7500 Fast Real-Time PCR system in triplicates using Power SYBR Green (ThermoFisher Scientific). .. Data were analyzed with integrated software, and expression levels were analyzed by the 2−∆∆Ct method after normalization to the housekeeping genes glutaraldehyde dehydrogenase (GAPDH).

    Concentration Assay:

    Article Title: Molecular subtyping of nasopharyngeal carcinoma (NPC) and a microRNA-based prognostic model for distant metastasis
    Article Snippet: .. RNA concentration was determined by NanoDrop One spectrophotometer (Thermo Fisher Scientific, USA). cDNA was reverse transcribed from 1 μg of total RNA using miScript II RT Kit (QIAGEN, USA). qPCR was carried out with miScript SYBR Green PCR Kit (QIAGEN, USA) on the LightCycler 480 System (Roche, Switzerland). hsa-miR-26a, hsa-miR-29b, hsa-miR-200b, hsa-miR-370, hsa-miR-622, hsa-miR-1248, hsa-miR-1293, hsa-miR-2053, hsa-let-7d and hsa-let-7 were predesigned primers (miScript Primer Assays MS00029239, MS00009289, MS00009023, MS00045885, MS00005117, MS00014238, MS00014539, MS00044569, MS00003136 and MS00006489, QIAGEN, USA). .. Amplification reactions were done in triplicate for each examined sample.

    Article Title: Investigation of candidate genes involved in the rhodoquinone biosynthetic pathway in Rhodospirillum rubrum
    Article Snippet: .. The concentration and quality of RNA (A260/280 ) was determined using a Nanodrop One UV-Vis spectrophotometer (Thermofisher Scientific, Waltham, MA). .. PCR with the Rru_A3231 in-gene primers ( 5’- TTCTGACCTTGCTGGCGATC-3’ and 3’- GGCTGATGCTGGCATCAAG-5’ ) was performed on each sample in order to test for genomic DNA contamination.

    Article Title: Pao Pereira extract suppresses benign prostatic hyperplasia by inhibiting inflammation-associated NFκB signaling
    Article Snippet: .. The concentration and purity of RNA were measured with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific), and RNA was kept at − 80 °C. .. Residual DNAs in total RNAs were removed and cDNAs were synthesized by Hifair II 1st Strand cDNA Synthesis SuperMix (11123ES60, YEASEN, Shanghai, China) following the manufacturer’s protocol.

    Article Title: Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632
    Article Snippet: .. RNA purity and concentration was assessed using the Nanodrop One UV-Vis Spectrophotometer (ThermoFischer Scientific, Waltham, MA). .. Library preparation was completed using the Illumina Neoprep Library Preparation System (Illumina, San Diego, CA).

    Polymerase Chain Reaction:

    Article Title: Molecular subtyping of nasopharyngeal carcinoma (NPC) and a microRNA-based prognostic model for distant metastasis
    Article Snippet: .. RNA concentration was determined by NanoDrop One spectrophotometer (Thermo Fisher Scientific, USA). cDNA was reverse transcribed from 1 μg of total RNA using miScript II RT Kit (QIAGEN, USA). qPCR was carried out with miScript SYBR Green PCR Kit (QIAGEN, USA) on the LightCycler 480 System (Roche, Switzerland). hsa-miR-26a, hsa-miR-29b, hsa-miR-200b, hsa-miR-370, hsa-miR-622, hsa-miR-1248, hsa-miR-1293, hsa-miR-2053, hsa-let-7d and hsa-let-7 were predesigned primers (miScript Primer Assays MS00029239, MS00009289, MS00009023, MS00045885, MS00005117, MS00014238, MS00014539, MS00044569, MS00003136 and MS00006489, QIAGEN, USA). .. Amplification reactions were done in triplicate for each examined sample.

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  • 89
    Thermo Fisher 1000 uv vis spectrophotometer
    Efficiency of genomic and plasmid DNA recovery with the QIAamp DNA mini kit columns. Genomic and plasmid DNA were isolated from E . coli DH5α and E . coli DH5α [pBR322] with the use of Genomic and Plasmid DNA mini kits, respectively (A A Biotechnology). DNA concentrations were measured by NanoDrop <t>1000</t> <t>UV-VIS</t> spectrophotometer (Thermo Scientific). Genomic DNA in the following amounts: 2110 ng, 1855 ng and 3922 ng, was coupled with 524 ng, 1100 ng and 1684 ng of plasmid DNA, respectively. Then, 100 μl of the lysis buffer (Qiagen) was added separately to genomic and plasmid DNA and the nucleic acids isolation was performed according to the QIAamp DNA mini kit manufacturer’s manual. The level of isolated DNA is indicated as a percentage relative to the unprocessed sample. The diagram represents three independent experiments. Error bars represent standard deviation (n = 3); (* P
    1000 Uv Vis Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1000 uv vis spectrophotometer/product/Thermo Fisher
    Average 89 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    1000 uv vis spectrophotometer - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    89
    Thermo Fisher 1000 spectrophotometric analysis
    Plant growth under P sufficient and P limiting conditions . Maize plants (var. B73) were grown for 21-days post emergence in 9L of inert sand substrate. From day 10, plants were fertilized with Hoagland solution (Hoagland and Broyer, 1936 ) containing either <t>1000</t> μM (left; +P) or 10 μM (right; –P) Pi.
    1000 Spectrophotometric Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1000 spectrophotometric analysis/product/Thermo Fisher
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    1000 spectrophotometric analysis - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    94
    Thermo Fisher nanodrop spectrophotometer nd 1000
    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a <t>NanoDrop</t> spectrophotometer <t>ND-1000.</t> B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.
    Nanodrop Spectrophotometer Nd 1000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop spectrophotometer nd 1000/product/Thermo Fisher
    Average 94 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    nanodrop spectrophotometer nd 1000 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Efficiency of genomic and plasmid DNA recovery with the QIAamp DNA mini kit columns. Genomic and plasmid DNA were isolated from E . coli DH5α and E . coli DH5α [pBR322] with the use of Genomic and Plasmid DNA mini kits, respectively (A A Biotechnology). DNA concentrations were measured by NanoDrop 1000 UV-VIS spectrophotometer (Thermo Scientific). Genomic DNA in the following amounts: 2110 ng, 1855 ng and 3922 ng, was coupled with 524 ng, 1100 ng and 1684 ng of plasmid DNA, respectively. Then, 100 μl of the lysis buffer (Qiagen) was added separately to genomic and plasmid DNA and the nucleic acids isolation was performed according to the QIAamp DNA mini kit manufacturer’s manual. The level of isolated DNA is indicated as a percentage relative to the unprocessed sample. The diagram represents three independent experiments. Error bars represent standard deviation (n = 3); (* P

    Journal: PLoS ONE

    Article Title: Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR

    doi: 10.1371/journal.pone.0169846

    Figure Lengend Snippet: Efficiency of genomic and plasmid DNA recovery with the QIAamp DNA mini kit columns. Genomic and plasmid DNA were isolated from E . coli DH5α and E . coli DH5α [pBR322] with the use of Genomic and Plasmid DNA mini kits, respectively (A A Biotechnology). DNA concentrations were measured by NanoDrop 1000 UV-VIS spectrophotometer (Thermo Scientific). Genomic DNA in the following amounts: 2110 ng, 1855 ng and 3922 ng, was coupled with 524 ng, 1100 ng and 1684 ng of plasmid DNA, respectively. Then, 100 μl of the lysis buffer (Qiagen) was added separately to genomic and plasmid DNA and the nucleic acids isolation was performed according to the QIAamp DNA mini kit manufacturer’s manual. The level of isolated DNA is indicated as a percentage relative to the unprocessed sample. The diagram represents three independent experiments. Error bars represent standard deviation (n = 3); (* P

    Article Snippet: DNA concentrations were measured by NanoDrop 1000 UV-VIS spectrophotometer (Thermo Scientific).

    Techniques: Plasmid Preparation, Isolation, Spectrophotometry, Lysis, Standard Deviation

    Plant growth under P sufficient and P limiting conditions . Maize plants (var. B73) were grown for 21-days post emergence in 9L of inert sand substrate. From day 10, plants were fertilized with Hoagland solution (Hoagland and Broyer, 1936 ) containing either 1000 μM (left; +P) or 10 μM (right; –P) Pi.

    Journal: Frontiers in Plant Science

    Article Title: The maize (Zea mays ssp. mays var. B73) genome encodes 33 members of the purple acid phosphatase family

    doi: 10.3389/fpls.2015.00341

    Figure Lengend Snippet: Plant growth under P sufficient and P limiting conditions . Maize plants (var. B73) were grown for 21-days post emergence in 9L of inert sand substrate. From day 10, plants were fertilized with Hoagland solution (Hoagland and Broyer, 1936 ) containing either 1000 μM (left; +P) or 10 μM (right; –P) Pi.

    Article Snippet: The integrity, quality and quantity of the RNA was assayed by agarose-electrophoresis, NanoDrop 1000 spectrophotometric analysis (Thermo Fisher Scientific Inc., DE, USA) and by using the Agilent Bioanalyzer system (Agilent Technologies Inc., CA, USA).

    Techniques:

    Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Journal: Veterinary Research

    Article Title: Visualizing bovine leukemia virus (BLV)-infected cells and measuring BLV proviral loads in the milk of BLV seropositive dams

    doi: 10.1186/s13567-019-0724-1

    Figure Lengend Snippet: Scatter diagrams showing the quantity and quality of DNA in blood and milk. DNA samples were obtained from 2 BLV-negative cattle in Farm 3, 43 BLV-infected cattle without lymphoma in Farms 2–6, and 3 BLV-infected cattle with lymphoma in Farm 1. A The average quantities of genomic DNA in blood and milk samples were 7.9 µg and 6.7 µg, respectively, as determined with a NanoDrop spectrophotometer ND-1000. B The average A260/A280 ratios of genomic DNA in blood and milk were 1.88 and 1.87, respectively, as determined using a NanoDrop spectrophotometer ND-1000. C The threshold cycle values of the blood and milk samples were 22.12 and 22.37, respectively, as indicated with the red bold lines.

    Article Snippet: The quantity and quality of DNA samples extracted from milk sample was determined based on the A260/280 ratio using a Nanodrop Spectrophotometer ND-1000 (Thermo Fisher Scientific).

    Techniques: Infection, Spectrophotometry