Journal: Advanced Science

Article Title: NCOA4 Accelerates Abdominal Aortic Aneurysm Formation by Regulating Macrophage Activation and Ferroptosis via STAT1–CH25H Axis

doi: 10.1002/advs.78079

Figure Lengend Snippet: Schematic representation of SI‐ Ch25h ‐LNP/MB for macrophage regulation in AAA. Under pathological stimulation, such as Ang II, NCOA4 in macrophages interacts with STAT1 to form a transcriptional complex that promotes CH25H transcription. The subsequent increase in 25‐HC production by CH25H activates macrophages, triggers inflammatory responses and ferroptosis, and accelerates the progression of AAA. Treatment with intravenous injection of SI‐ Ch25h ‐LNP/MB combined with localized ultrasound bursts can suppress CH25H expression in macrophages within the abdominal aortic wall, thereby offering a therapeutic strategy for AAA. 25‐HC, 25‐hydroxycholesterol; AAA, abdominal aortic aneurysm; Ang II, angiotensin II; CH25H, cholesterol 25‐hydroxylase; LNP/MB, lipid nanoparticle loaded microbubble; NCOA4, nuclear receptor coactivator 4; STAT1, signal transducer and activator of transcription 1. Created in BioRender. P, X. (2026) https://BioRender.com/4q3b4g4 .

Article Snippet: The zeta potential of SI‐ Ch25h ‐LNP, MB, and SI‐ Ch25h ‐LNP/MB was analyzed using nanoparticle tracking analysis (Zetasizer Lab, Malvern).

Techniques: Injection, Expressing

Journal: Advanced Science

Article Title: NCOA4 Accelerates Abdominal Aortic Aneurysm Formation by Regulating Macrophage Activation and Ferroptosis via STAT1–CH25H Axis

doi: 10.1002/advs.78079

Figure Lengend Snippet: Targeting CH25H via LNP/MBs Attenuates AAA Progression. (A) Diagram of experimental procedure: ApoE −/− mice were randomly divided into 2 groups and subjected to mini‐osmotic pump implantation, infused with Ang II (1000 ng/kg/min) or normal saline. 7 days after operation, the mice infused with Ang II or normal saline were randomly divided into 2 groups again and injected with SI‐NC‐LNP/MB or SI‐ Ch25h ‐LNP/MB. All mice were fed a Western diet during the procedure. (B) Representative macroscopic and echocardiographic images of abdominal aortas in indicated groups. Scale bars indicate 4 mm (macroscopic image) and 1 mm (echocardiographic image). (C) Survival curves in indicated groups (n = 8 per group infused with Saline, n = 16–20 per group infused with Ang II). Survival rates were analyzed by the Kaplan–Meier method and compared using log‐rank tests. (In the SI‐NC‐LNP/MB+Ang II group, 5 mice died with isolated abdominal aortic rupture, and 1 mouse died with combined thoracic/abdominal aortic rupture prior to the termination; in the SI‐ Ch25h ‐LNP/MB group, 4 mice died with isolated abdominal aortic rupture). (D) Incidence of AAA induced by Saline or Ang II in indicated groups (n = 8 per group infused with Saline, n = 14–15 per group infused with Ang II). Data were analyzed by a Fisher exact test. (E) Quantification of the maximal diameter of suprarenal abdominal aortas using a caliper at sacrifice (n = 8 per group infused with Saline, n = 14–15 per group infused with Ang II). Data were expressed as the mean ± SEM and analyzed by two‐way ANOVA following Tukey's multiple comparisons. (F) Representative images of HE, Masson, and EVG staining of cross‐sections of abdominal aortas. Scale bar indicates 100 µm. (G) Grade of elastin degradation (n = 8 per group). Data were expressed as median with interquartile range and analyzed by nonparametric Kruskal–Wallis test with Dunn's post hoc test. (H) Quantitative analysis of collagen deposition (n = 10 per group). Data were expressed as the mean ± SEM and analyzed by two‐way ANOVA followed by Tukey's multiple comparisons. ns indicates no significance, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. AAA, abdominal aortic aneurysm; Ang II, angiotensin II; EVG, elastic Van Gieson staining; HE, hematoxylin‐eosin staining; LNP/MB, lipid nanoparticle‐loaded microbubble.

Article Snippet: The zeta potential of SI‐ Ch25h ‐LNP, MB, and SI‐ Ch25h ‐LNP/MB was analyzed using nanoparticle tracking analysis (Zetasizer Lab, Malvern).

Techniques: Saline, Injection, Western Blot, Isolation, Staining, Analysis, H&E Stain

Journal: RSC Advances

Article Title: Production and characterization of structural nanocellulose from young palm fruit residues for reinforcement in bionanocomposite fabrication: from waste to nanomaterials

doi: 10.1039/d6ra05304a

Figure Lengend Snippet: Comparative FE-SEM images of the (a) raw fiber (RF), (b) alkali-treated fiber (ATF), (c) bleached fiber (BF), and (d) cellulose nanocrystals (CNCs) extracted from locally available young palm fruit residue-derived lignocellulosic fibers after harvesting the edible parts ( e.g. , ice apples).

Article Snippet: In this research work, a HORIBA Scientific Nano Partica SZ-100V2 nanoparticle analyzer (manufactured in Japan) located at the Fiber and Polymer Research Division, BCSIR Rajshahi Laboratories, Rajshahi, Bangladesh, was used to determine the particle size distribution of the produced CNCs.

Techniques: Residue, Derivative Assay