nano hplc  (Agilent technologies)

 
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    Name:
    Capillary Nano
    Description:
    ZORBAX capillary 0 5 mm 0 3 mm id and nano 0 1 mm 0 075 mm id columns are available in a wide range of phases pore sizes 80 Å and 300 Å and dimensions These columns are particularly well suited for 1D and 2D proteomics applications By reducing on column dilution capillary and nano columns deliver the enhanced sensitivity and exceptional reproducibility required by the most sample limited applications
    Catalog Number:
    PL1312-1300
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    None
    Category:
    Products Biopharma Hplc Analysis Peptide Mapping Capillary Nano
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    Structured Review

    Agilent technologies nano hplc
    HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography <t>(RP-HPLC)</t> to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by <t>nano-HPLC</t> coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.
    ZORBAX capillary 0 5 mm 0 3 mm id and nano 0 1 mm 0 075 mm id columns are available in a wide range of phases pore sizes 80 Å and 300 Å and dimensions These columns are particularly well suited for 1D and 2D proteomics applications By reducing on column dilution capillary and nano columns deliver the enhanced sensitivity and exceptional reproducibility required by the most sample limited applications
    https://www.bioz.com/result/nano hplc/product/Agilent technologies
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano hplc - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Paneth cell α-defensin 6 (HD-6) is an antimicrobial peptide"

    Article Title: Paneth cell α-defensin 6 (HD-6) is an antimicrobial peptide

    Journal: Mucosal Immunology

    doi: 10.1038/mi.2014.100

    HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by nano-HPLC coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.
    Figure Legend Snippet: HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by nano-HPLC coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.

    Techniques Used: Incubation, Mass Spectrometry, High Performance Liquid Chromatography, Derivative Assay

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    Article Snippet: .. HPLC-ESI-MS: The chromatography conditions described above were used with an Agilent PLRP-S PSDVB column (PL1312-1300) and 1290 HPLC system. ..

    Article Title: Decarboxylation involving a ferryl, propionate, and a tyrosyl group in a radical relay yields heme b
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    Chromatography:

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    Reversed-phase Chromatography:

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    Article Snippet: .. The peptide mixtures were loaded onto a Zorbax 300SB-C18 pre-column (Agilent Technologies, Cat # 5065-9913) and were separated by reversed-phase chromatography using a 12 cm column with an inner diameter of 75 μm, packed with 5 μm C18 particles (Nikkyo Technos Co., Ltd., Tokyo, Japan). ..

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    Agilent technologies nano hplc
    HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography <t>(RP-HPLC)</t> to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by <t>nano-HPLC</t> coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.
    Nano Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc/product/Agilent technologies
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano hplc - by Bioz Stars, 2021-09
    94/100 stars
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    94
    Agilent technologies nano hplc system 1100
    MS/MS spectrum of the phosphopeptide GQSTVS#SELK from GDF-9. After isolation of the phosphopeptides by TiO2 affinity chromatography, the analysis was carried out with an Ion trap mass spectrometer coupled to a <t>nano-HPLC.</t> In the spectrum are evident the
    Nano Hplc System 1100, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc system 1100/product/Agilent technologies
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano hplc system 1100 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    93
    Agilent technologies nano flow high performance liquid chromatography hplc system
    MS/MS spectrum of the phosphopeptide GQSTVS#SELK from GDF-9. After isolation of the phosphopeptides by TiO2 affinity chromatography, the analysis was carried out with an Ion trap mass spectrometer coupled to a <t>nano-HPLC.</t> In the spectrum are evident the
    Nano Flow High Performance Liquid Chromatography Hplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano flow high performance liquid chromatography hplc system/product/Agilent technologies
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano flow high performance liquid chromatography hplc system - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

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    HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by nano-HPLC coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.

    Journal: Mucosal Immunology

    Article Title: Paneth cell α-defensin 6 (HD-6) is an antimicrobial peptide

    doi: 10.1038/mi.2014.100

    Figure Lengend Snippet: HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by nano-HPLC coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.

    Article Snippet: Chromatographic separation was carried out by nano-HPLC (ChipCube, Agilent) with a reversed-phase column using an Agilent HPLC ProtID-Chip-43 with a 40 nl enrichment column and a 75 μl × 43 mm analytical column made of ZORBAX 300SB-C18 5 μm material.

    Techniques: Incubation, Mass Spectrometry, High Performance Liquid Chromatography, Derivative Assay

    MS/MS spectrum of the phosphopeptide GQSTVS#SELK from GDF-9. After isolation of the phosphopeptides by TiO2 affinity chromatography, the analysis was carried out with an Ion trap mass spectrometer coupled to a nano-HPLC. In the spectrum are evident the

    Journal: FEBS letters

    Article Title: Golgi apparatus casein kinase phosphorylates bioactive Ser-6 of bone morphogenetic protein 15 and growth and differentiation factor 9

    doi: 10.1016/j.febslet.2009.12.052

    Figure Lengend Snippet: MS/MS spectrum of the phosphopeptide GQSTVS#SELK from GDF-9. After isolation of the phosphopeptides by TiO2 affinity chromatography, the analysis was carried out with an Ion trap mass spectrometer coupled to a nano-HPLC. In the spectrum are evident the

    Article Snippet: Phosphopeptides were enriched according to [ ] using in-house made microcolumns of TiO2 and analyzed using a LCQ XP mass spectrometer (Thermo Scientific) coupled to a nano-HPLC system 1100 (Agilent Technologies) for the rhGDF-9 samples, whereas the rhBMP-15 samples were analyzed with a 4800 Plus MALDI TOF/TOF (Applied Biosystems).

    Techniques: Mass Spectrometry, Isolation, Affinity Chromatography, High Performance Liquid Chromatography