nano hplc system  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher nano hplc system
    <t>Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS</t> analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc system/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nano hplc system - by Bioz Stars, 2020-03
    99/100 stars

    Images

    1) Product Images from "Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes"

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0112886

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Figure Legend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay

    Related Articles

    Centrifugation:

    Article Title: Neisseria gonorrhoeae MlaA influences gonococcal virulence and membrane vesicle production
    Article Snippet: Fractionated samples were hydrated in 0.1% TFA to load onto the cartridge, washed with 0.1% TFA, eluted in 1 mL 70% ACN/0.1% TFA, and dried by vacuum centrifugation. .. Desalted samples were subsequently analyzed by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA) mass spectrometer at the Proteomic Core at The Fred Hutchinson Cancer Research Center, Seattle, WA.

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: Peptide fraction was recovered as supernatants by centrifugation at 21,500 × g for 15 min at 4 °C. .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker).

    Zymography:

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: Identification of the Exoprotease Produced Under Starving Conditions In order to identify the proteases produced by the strains PA14 and P729, casein zymography was done as described ( ). .. Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm).

    Incubation:

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: Pit2 and PID14 peptide MS analysis Recombinant UmPit2 (1 µg) and PID14 peptide (20 µM) were incubated with F24 fractions for 30 min at RT. .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker).

    Mass Spectrometry:

    Article Title: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
    Article Snippet: .. The samples then underwent shotgun proteomic analysis on a nano-HPLC system (NanoLC 2D; Eksigent, Dublin, CA) coupled to a hybrid mass spectrometer (Orbitrap Velos Pro; Thermo-Electron, Bremen, Germany). .. Samples were injected using an auto-sampler and loaded onto a self-packed trap column (2 cm, 100 µm ID, packed with C18 Magic AQ from Michrom Bioresources, Auburn, CA), and the samples were then analyzed on a self-packed C18 (15 cm, 2.7 µm HALO Peptide ES C-18, MAC-MOD, Chadds Ford, PA) column with a laser-pulled tip (P-2000, Sutter, Novato, CA) using a flow rate of 200nL/min.

    Article Title: Identification of Novel Substrates for the Serine Protease HTRA1 in the Human RPE Secretome
    Article Snippet: .. LC-MS/MS was performed on a nano-HPLC system (NanoLC 2D; Eksignet, Dublin, CA) connected to a hybrid mass spectrometer (LTQ/Orbitrap; Thermo Fisher Scientific, Inc., San Jose, CA). .. Eluting peptides were introduced into the mass spectrometer via a 10-μm silica tip (New Objective Inc., Ringoes, NJ) adapted to a nano-electrospray source (Thermo Fisher Scientific, Inc.).

    Article Title: Neisseria gonorrhoeae MlaA influences gonococcal virulence and membrane vesicle production
    Article Snippet: .. Desalted samples were subsequently analyzed by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA) mass spectrometer at the Proteomic Core at The Fred Hutchinson Cancer Research Center, Seattle, WA. .. Samples were further desalted in-line using a reversed-phase trap column (100 μm × 20 mm) packed with Magic C18 AQ (5 μm 200 Å resin; Michrom Bioresources) and peptides were separated on a reversed phase column directly mounted to the electrospray ion source [75 μm × 250 mm column packed with Magic C18 AQ resin (5 μm 200 Å resin; Michrom Bioresources)].

    Article Title: The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease
    Article Snippet: .. The samples were identified with a nano-HPLC system (LC Packings, Dionex), which was directly coupled with a Q-Tof1 (Micromass, Waters) mass spectrometer. .. The separated peptides coming from the C18 PepMap column (Dionex) were selected automatically for low energy collision-induced dissociation experiments.

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker). ..

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm). .. Electrospray ionization of the eluted peptides was performed with a CaptiveSpray source (Bruker) assisted by a flow of nitrogen boiled on acetonitrile (0.2 bar) and the mass spectra were acquired with a quadrupole time-of-flight mass spectrometer (Impact II, Bruker).

    Article Title: Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens
    Article Snippet: .. The resulting peptide solution was separated using the Dionex Ultimate 3,000 nano-HPLC system (ThermoFischer Scientific, USA) and then analyzed using a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFischer Scientific, USA). .. The spectra generated by LC-MS were analyzed with Byonic Software (Protein Metrics USA) using publically available sequences retrieved from UniProt ( www.uniprot.org ).

    Article Title: Crucial role of calbindin-D28k in the pathogenesis of Alzheimer's disease mouse model
    Article Snippet: .. LC-MS/MS analysis Peptides isolated from the in-gel digestion were resuspended in 50 μ l of solvent A (2% acetonitrile in 0.1% formic acid) and 2 μ l of sample was injected into nano-HPLC system (Easy nLC; Thermo Fisher Scientific, San Jose, CA, USA) and LTQ-Velos mass spectrometer (Thermo Fisher Scientific). .. The sample was loaded onto an in-house packed 75 μ m (inner diameter) × 10 cm C18 column and separated with a linear gradient of 2–38% solvent B (98% acetonitrile in 0.1% formic acid) for 90 min at a flow rate of 300 nl/min.

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes
    Article Snippet: .. The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific). .. Data were acquired in data-dependent MS/MS mode: Each high-resolution full scan (m/z 350 to 2000, R = 60,000) in the orbitrap was followed by five product ion scans in the LTQ (collision-induced dissociation with 35% normalized collision energy) of the five most intense signals in the full-scan mass spectrum (isolation window 1.5 u).

    Article Title: Identification of Enolase as the Target of 2-Aminothiazoles in Mycobacterium tuberculosis
    Article Snippet: .. Desalted peptide samples were resuspended in 10 μL 2% acetonitrile/0.1% formic acid and were analyzed (8 μL) by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA, United States) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA, United States) mass spectrometer. .. In-line de-salting was accomplished using a reversed-phase trap column (100 μm × 20 mm) packed with Magic C18 AQ (5-μm 200 Å resin; Michrom Bioresources, Bruker, Billerica, MA) followed by peptide separations on a reversed-phase column (75 μm × 250 mm) packed with Magic C18 AQ (5-μm 100Å resin; Michrom Bioresources, Bruker, Billerica, MA, United States) directly mounted on the electrospray ion source.

    Article Title: RNA binding to APOBEC3G induces the disassembly of functional deaminase complexes by displacing single-stranded DNA substrates
    Article Snippet: .. MS analysis of tryptic peptides was performed using LTQ Orbitrap XL (Thermo Fisher Scientific) analysis, a nano-HPLC system (Easy-nLC II, Thermo Fisher Scientific) coupled to the electrospray ionization source of an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific). ..

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: .. Separation was performed with a binary gradient from 5 to 32% B for 90 min with a total runtime of 2 h per sample on a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 15 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker) as described by ref. . .. The Bruker HyStar Software (v3.2) was used to acquire line-mode MS spectra in a mass range from 200 to 1750 m/z at an acquisition rate of 4 Hz and the Top17 most intense ions were selected for fragmentation.

    Article Title: Cell-Cycle-Dependent Structural Transitions in the Human CENP-A Nucleosome In Vivo
    Article Snippet: .. LC-MS/MS was performed on nano-HPLC system (Proxeon EASY; ThermoElectron, Bremen, Germany) connected to a hybrid mass spectrometer (Velos/Orbitrap; ThermoElectron, Bremen, Germany). .. Each sample was injected via an auto-sampler and directly loaded on to packed analytical column at the flow rate of 1.2ul/min and the sample was subsequently separated at the flow rate of 400 nl/min.

    High Performance Liquid Chromatography:

    Article Title: Neisseria gonorrhoeae MlaA influences gonococcal virulence and membrane vesicle production
    Article Snippet: .. Desalted samples were subsequently analyzed by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA) mass spectrometer at the Proteomic Core at The Fred Hutchinson Cancer Research Center, Seattle, WA. .. Samples were further desalted in-line using a reversed-phase trap column (100 μm × 20 mm) packed with Magic C18 AQ (5 μm 200 Å resin; Michrom Bioresources) and peptides were separated on a reversed phase column directly mounted to the electrospray ion source [75 μm × 250 mm column packed with Magic C18 AQ resin (5 μm 200 Å resin; Michrom Bioresources)].

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: .. Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm). .. Chromatographic runs were performed at a constant flow of 250 nL/min of a mix of 0.1% (v/v) FA in water (Buffer A), and 0.1% (v/v) FA in HPLC grade acetonitrile (Buffer B) in a linear gradient of 50 min from 1 to 50% B.

    Article Title: Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species
    Article Snippet: .. Sample (10 μL) from each fraction was injected 3 times to the Nano HPLC system. .. Peptides were separated on a C18 analytical reverse phase column (75 μm × 12 cm, 3 μm, 200 Å, Thermo, USA) at a flow rate of 350 nL/min and a linear LC gradient profile was used to elute peptides from the column.

    Article Title: Identification of Enolase as the Target of 2-Aminothiazoles in Mycobacterium tuberculosis
    Article Snippet: .. Desalted peptide samples were resuspended in 10 μL 2% acetonitrile/0.1% formic acid and were analyzed (8 μL) by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA, United States) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA, United States) mass spectrometer. .. In-line de-salting was accomplished using a reversed-phase trap column (100 μm × 20 mm) packed with Magic C18 AQ (5-μm 200 Å resin; Michrom Bioresources, Bruker, Billerica, MA) followed by peptide separations on a reversed-phase column (75 μm × 250 mm) packed with Magic C18 AQ (5-μm 100Å resin; Michrom Bioresources, Bruker, Billerica, MA, United States) directly mounted on the electrospray ion source.

    Flow Cytometry:

    Article Title: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
    Article Snippet: The samples then underwent shotgun proteomic analysis on a nano-HPLC system (NanoLC 2D; Eksigent, Dublin, CA) coupled to a hybrid mass spectrometer (Orbitrap Velos Pro; Thermo-Electron, Bremen, Germany). .. Samples were injected using an auto-sampler and loaded onto a self-packed trap column (2 cm, 100 µm ID, packed with C18 Magic AQ from Michrom Bioresources, Auburn, CA), and the samples were then analyzed on a self-packed C18 (15 cm, 2.7 µm HALO Peptide ES C-18, MAC-MOD, Chadds Ford, PA) column with a laser-pulled tip (P-2000, Sutter, Novato, CA) using a flow rate of 200nL/min.

    Article Title: Neisseria gonorrhoeae MlaA influences gonococcal virulence and membrane vesicle production
    Article Snippet: The sample was brought up in 200 μL buffer A. Peptides were separated over 60 min with a 2.1 mm x 100 mm Polysulfoethyl A column (PolyLC) at a 200 μL/min flow rate using the following separation profile: hold 2% B for 5 min, 2% to 8% B in 0.1 min, 8% to 18% B in 14.9 min, 18% to 34% B in 12 min, 34% to 60% B in 18 min, 60% to 98% B in 0.1 min and hold for 10 min. We collected 1 min fractions in 96-well microtiter plates. .. Desalted samples were subsequently analyzed by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA) mass spectrometer at the Proteomic Core at The Fred Hutchinson Cancer Research Center, Seattle, WA.

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm). .. Chromatographic runs were performed at a constant flow of 250 nL/min of a mix of 0.1% (v/v) FA in water (Buffer A), and 0.1% (v/v) FA in HPLC grade acetonitrile (Buffer B) in a linear gradient of 50 min from 1 to 50% B.

    Article Title: Evaluation of the Compact High-Field Orbitrap for Top-Down Proteomics of Human Cells
    Article Snippet: The nano-HPLC system (Dionex, Thermo Fisher, Sunnyvale, CA) was operated at 2.5 µL/min for loading onto the trap for 10 min. .. Afterwards, proteins were eluted at a constant flow rate of 300 nL/min using the following gradient: 5% B (95% acetonitrile, 5% water, 0.2% formic acid) at start, 25% at 5 min, 60% B at 45 min, 80% B at 50 min, 5% B at 60 min.

    Article Title: Crucial role of calbindin-D28k in the pathogenesis of Alzheimer's disease mouse model
    Article Snippet: LC-MS/MS analysis Peptides isolated from the in-gel digestion were resuspended in 50 μ l of solvent A (2% acetonitrile in 0.1% formic acid) and 2 μ l of sample was injected into nano-HPLC system (Easy nLC; Thermo Fisher Scientific, San Jose, CA, USA) and LTQ-Velos mass spectrometer (Thermo Fisher Scientific). .. The sample was loaded onto an in-house packed 75 μ m (inner diameter) × 10 cm C18 column and separated with a linear gradient of 2–38% solvent B (98% acetonitrile in 0.1% formic acid) for 90 min at a flow rate of 300 nl/min.

    Article Title: Identification of Enolase as the Target of 2-Aminothiazoles in Mycobacterium tuberculosis
    Article Snippet: Desalted peptide samples were resuspended in 10 μL 2% acetonitrile/0.1% formic acid and were analyzed (8 μL) by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA, United States) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA, United States) mass spectrometer. .. A 45-min gradient from 7 to 35% acetonitrile in 0.1% formic acid at a flow rate of 400 nL/min was used for chromatographic separations.

    Article Title: Cell-Cycle-Dependent Structural Transitions in the Human CENP-A Nucleosome In Vivo
    Article Snippet: LC-MS/MS was performed on nano-HPLC system (Proxeon EASY; ThermoElectron, Bremen, Germany) connected to a hybrid mass spectrometer (Velos/Orbitrap; ThermoElectron, Bremen, Germany). .. Each sample was injected via an auto-sampler and directly loaded on to packed analytical column at the flow rate of 1.2ul/min and the sample was subsequently separated at the flow rate of 400 nl/min.

    Chromatography:

    Article Title: Identification of Novel Substrates for the Serine Protease HTRA1 in the Human RPE Secretome
    Article Snippet: Paragraph title: Liquid Chromatography-Tandem Mass Spectrometry ... LC-MS/MS was performed on a nano-HPLC system (NanoLC 2D; Eksignet, Dublin, CA) connected to a hybrid mass spectrometer (LTQ/Orbitrap; Thermo Fisher Scientific, Inc., San Jose, CA).

    Article Title: Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens
    Article Snippet: Paragraph title: Protein Identity Determination by Liquid Chromatography-Mass Spectrometry (LC-MS) ... The resulting peptide solution was separated using the Dionex Ultimate 3,000 nano-HPLC system (ThermoFischer Scientific, USA) and then analyzed using a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFischer Scientific, USA).

    Article Title: Cell-Cycle-Dependent Structural Transitions in the Human CENP-A Nucleosome In Vivo
    Article Snippet: Paragraph title: Liquid Chromatography-Tandem Mass Spectrometry ... LC-MS/MS was performed on nano-HPLC system (Proxeon EASY; ThermoElectron, Bremen, Germany) connected to a hybrid mass spectrometer (Velos/Orbitrap; ThermoElectron, Bremen, Germany).

    SDS Page:

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: To determine the zone of migration of casein exoproteases, in parallel, SDS-PAGE using the same conditions was performed, except casein was added to the gel, and the bands of interest were cut with a scalpel in pieces of approximately 1 mm3 , and thoroughly washed with (i) 25 mM ammonium bicarbonate (ABC), 50% acetonitrile (ACN); (ii) 100% ACN; (iii) 50 mM ABC; and (iv) 100% ACN. .. Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm).

    Article Title: Identification of Enolase as the Target of 2-Aminothiazoles in Mycobacterium tuberculosis
    Article Snippet: Bands of interest were extracted from both the 2-AT and control lanes of the SDS-PAGE gels and digested using the In-Gel Tryptic Digestion Kit (Thermo Fisher Scientific) according to manufacturer’s instructions. .. Desalted peptide samples were resuspended in 10 μL 2% acetonitrile/0.1% formic acid and were analyzed (8 μL) by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA, United States) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA, United States) mass spectrometer.

    Article Title: RNA binding to APOBEC3G induces the disassembly of functional deaminase complexes by displacing single-stranded DNA substrates
    Article Snippet: Preparation of A3G for mass spectrometry Nucleic acid-free A3G and A3G cross-linked to nucleic acid were cut from SDS-PAGE gels and washed three times in 20 mM Tris pH 7.5 2.5 mM MgCl2 (for ssDNA cross-linking) or in 25 mM Tris pH 7.5 2 mM EDTA (for RNA cross-linking) and processed as described in Supplemental Methods. .. MS analysis of tryptic peptides was performed using LTQ Orbitrap XL (Thermo Fisher Scientific) analysis, a nano-HPLC system (Easy-nLC II, Thermo Fisher Scientific) coupled to the electrospray ionization source of an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific).

    Generated:

    Article Title: Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens
    Article Snippet: The resulting peptide solution was separated using the Dionex Ultimate 3,000 nano-HPLC system (ThermoFischer Scientific, USA) and then analyzed using a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFischer Scientific, USA). .. The spectra generated by LC-MS were analyzed with Byonic Software (Protein Metrics USA) using publically available sequences retrieved from UniProt ( www.uniprot.org ).

    Inhibition:

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: Samples were preincubated with E-64 for inhibition of PLCPs or alternative inhibitor mix (EDTA 1 mM, Pepstatin A 30 µM, DCI 30 µM) for 10 min. After addition of final 3 M guanidinium hydrochloride, samples were precipitated with 10% v/v TCA o/N at 4 °C. .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker).

    Tandem Mass Spectroscopy:

    Article Title: Identification of Novel Substrates for the Serine Protease HTRA1 in the Human RPE Secretome
    Article Snippet: LC-MS/MS was performed on a nano-HPLC system (NanoLC 2D; Eksignet, Dublin, CA) connected to a hybrid mass spectrometer (LTQ/Orbitrap; Thermo Fisher Scientific, Inc., San Jose, CA). .. The mass spectrometer was operated in data-dependent mode in which one cycle of experiments consisted of one full-MS survey followed by three sequential pairs of intercalated MS/MS experiments.

    Article Title: Neisseria gonorrhoeae MlaA influences gonococcal virulence and membrane vesicle production
    Article Snippet: .. Desalted samples were subsequently analyzed by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA) mass spectrometer at the Proteomic Core at The Fred Hutchinson Cancer Research Center, Seattle, WA. .. Samples were further desalted in-line using a reversed-phase trap column (100 μm × 20 mm) packed with Magic C18 AQ (5 μm 200 Å resin; Michrom Bioresources) and peptides were separated on a reversed phase column directly mounted to the electrospray ion source [75 μm × 250 mm column packed with Magic C18 AQ resin (5 μm 200 Å resin; Michrom Bioresources)].

    Article Title: The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease
    Article Snippet: The samples were identified with a nano-HPLC system (LC Packings, Dionex), which was directly coupled with a Q-Tof1 (Micromass, Waters) mass spectrometer. .. The resulting tandem mass spectrometry (MS/MS) fragmentation spectra were used for the analysis and for identification of the protein sample.

    Article Title: Crucial role of calbindin-D28k in the pathogenesis of Alzheimer's disease mouse model
    Article Snippet: LC-MS/MS analysis Peptides isolated from the in-gel digestion were resuspended in 50 μ l of solvent A (2% acetonitrile in 0.1% formic acid) and 2 μ l of sample was injected into nano-HPLC system (Easy nLC; Thermo Fisher Scientific, San Jose, CA, USA) and LTQ-Velos mass spectrometer (Thermo Fisher Scientific). .. The LTQ was operated in data-dependent mode with one survey MS scan at the mass range 400–1400 m/z and followed by five MS/MS scans.

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes
    Article Snippet: The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific). .. Data were acquired in data-dependent MS/MS mode: Each high-resolution full scan (m/z 350 to 2000, R = 60,000) in the orbitrap was followed by five product ion scans in the LTQ (collision-induced dissociation with 35% normalized collision energy) of the five most intense signals in the full-scan mass spectrum (isolation window 1.5 u).

    Article Title: Identification of Enolase as the Target of 2-Aminothiazoles in Mycobacterium tuberculosis
    Article Snippet: .. Desalted peptide samples were resuspended in 10 μL 2% acetonitrile/0.1% formic acid and were analyzed (8 μL) by LC/ESI MS/MS with a Thermo Scientific Easy-nLC II (Thermo Scientific, Waltham, MA, United States) nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA, United States) mass spectrometer. .. In-line de-salting was accomplished using a reversed-phase trap column (100 μm × 20 mm) packed with Magic C18 AQ (5-μm 200 Å resin; Michrom Bioresources, Bruker, Billerica, MA) followed by peptide separations on a reversed-phase column (75 μm × 250 mm) packed with Magic C18 AQ (5-μm 100Å resin; Michrom Bioresources, Bruker, Billerica, MA, United States) directly mounted on the electrospray ion source.

    Article Title: RNA binding to APOBEC3G induces the disassembly of functional deaminase complexes by displacing single-stranded DNA substrates
    Article Snippet: MS analysis of tryptic peptides was performed using LTQ Orbitrap XL (Thermo Fisher Scientific) analysis, a nano-HPLC system (Easy-nLC II, Thermo Fisher Scientific) coupled to the electrospray ionization source of an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific). .. Search parameters included: trypsin as an enzyme; three missed cleavages; 0.05 Da for MS and MS/MS data from the LTQ Obitrap XL (1 for #13C, +2; +3 for charge state), decoy search and acceptance criteria of a minimum one peptide greater than identity score (minimum score of 15).

    Sonication:

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: After washing the gel pieces, digestion were made with 25 ng/μL trypsin in the minimum volume of 25 mM ABC necessary to cover the gel pieces for 24 h. The peptide extraction was performed in two steps: the first extraction was made with 200 μL of 25% ACN, 1% FA during 15 min stirring every 5 min; the second extraction was made with 200 μL of 50% ACN, 1% FA for 30 min and then sonicated for 5 min. .. Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm).

    Injection:

    Article Title: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
    Article Snippet: The samples then underwent shotgun proteomic analysis on a nano-HPLC system (NanoLC 2D; Eksigent, Dublin, CA) coupled to a hybrid mass spectrometer (Orbitrap Velos Pro; Thermo-Electron, Bremen, Germany). .. Samples were injected using an auto-sampler and loaded onto a self-packed trap column (2 cm, 100 µm ID, packed with C18 Magic AQ from Michrom Bioresources, Auburn, CA), and the samples were then analyzed on a self-packed C18 (15 cm, 2.7 µm HALO Peptide ES C-18, MAC-MOD, Chadds Ford, PA) column with a laser-pulled tip (P-2000, Sutter, Novato, CA) using a flow rate of 200nL/min.

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker). ..

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: .. Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm). .. Chromatographic runs were performed at a constant flow of 250 nL/min of a mix of 0.1% (v/v) FA in water (Buffer A), and 0.1% (v/v) FA in HPLC grade acetonitrile (Buffer B) in a linear gradient of 50 min from 1 to 50% B.

    Article Title: Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species
    Article Snippet: .. Sample (10 μL) from each fraction was injected 3 times to the Nano HPLC system. .. Peptides were separated on a C18 analytical reverse phase column (75 μm × 12 cm, 3 μm, 200 Å, Thermo, USA) at a flow rate of 350 nL/min and a linear LC gradient profile was used to elute peptides from the column.

    Article Title: Crucial role of calbindin-D28k in the pathogenesis of Alzheimer's disease mouse model
    Article Snippet: .. LC-MS/MS analysis Peptides isolated from the in-gel digestion were resuspended in 50 μ l of solvent A (2% acetonitrile in 0.1% formic acid) and 2 μ l of sample was injected into nano-HPLC system (Easy nLC; Thermo Fisher Scientific, San Jose, CA, USA) and LTQ-Velos mass spectrometer (Thermo Fisher Scientific). .. The sample was loaded onto an in-house packed 75 μ m (inner diameter) × 10 cm C18 column and separated with a linear gradient of 2–38% solvent B (98% acetonitrile in 0.1% formic acid) for 90 min at a flow rate of 300 nl/min.

    Article Title: Cell-Cycle-Dependent Structural Transitions in the Human CENP-A Nucleosome In Vivo
    Article Snippet: LC-MS/MS was performed on nano-HPLC system (Proxeon EASY; ThermoElectron, Bremen, Germany) connected to a hybrid mass spectrometer (Velos/Orbitrap; ThermoElectron, Bremen, Germany). .. Each sample was injected via an auto-sampler and directly loaded on to packed analytical column at the flow rate of 1.2ul/min and the sample was subsequently separated at the flow rate of 400 nl/min.

    Recombinant:

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: Pit2 and PID14 peptide MS analysis Recombinant UmPit2 (1 µg) and PID14 peptide (20 µM) were incubated with F24 fractions for 30 min at RT. .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker).

    Isolation:

    Article Title: Crucial role of calbindin-D28k in the pathogenesis of Alzheimer's disease mouse model
    Article Snippet: .. LC-MS/MS analysis Peptides isolated from the in-gel digestion were resuspended in 50 μ l of solvent A (2% acetonitrile in 0.1% formic acid) and 2 μ l of sample was injected into nano-HPLC system (Easy nLC; Thermo Fisher Scientific, San Jose, CA, USA) and LTQ-Velos mass spectrometer (Thermo Fisher Scientific). .. The sample was loaded onto an in-house packed 75 μ m (inner diameter) × 10 cm C18 column and separated with a linear gradient of 2–38% solvent B (98% acetonitrile in 0.1% formic acid) for 90 min at a flow rate of 300 nl/min.

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes
    Article Snippet: The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific). .. Data were acquired in data-dependent MS/MS mode: Each high-resolution full scan (m/z 350 to 2000, R = 60,000) in the orbitrap was followed by five product ion scans in the LTQ (collision-induced dissociation with 35% normalized collision energy) of the five most intense signals in the full-scan mass spectrum (isolation window 1.5 u).

    Size-exclusion Chromatography:

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes
    Article Snippet: The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific). .. Dynamic exclusion (exclusion duration 120 sec, exclusion window −1 to 2 u) was enabled to allow detection of less abundant ions.

    Purification:

    Article Title: The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease
    Article Snippet: Mass spectrometry Identification of the purified XSP10 protein was done with the in-gel digestion method as described ( ). .. The samples were identified with a nano-HPLC system (LC Packings, Dionex), which was directly coupled with a Q-Tof1 (Micromass, Waters) mass spectrometer.

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: .. Supernatants were purified by C18 StageTips and injected onto a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 50 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker). ..

    Liquid Chromatography:

    Article Title: Evaluation of the Compact High-Field Orbitrap for Top-Down Proteomics of Human Cells
    Article Snippet: Paragraph title: Reversed-phase Nano-capillary Liquid Chromatography ... The nano-HPLC system (Dionex, Thermo Fisher, Sunnyvale, CA) was operated at 2.5 µL/min for loading onto the trap for 10 min.

    Software:

    Article Title: Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens
    Article Snippet: The resulting peptide solution was separated using the Dionex Ultimate 3,000 nano-HPLC system (ThermoFischer Scientific, USA) and then analyzed using a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFischer Scientific, USA). .. The spectra generated by LC-MS were analyzed with Byonic Software (Protein Metrics USA) using publically available sequences retrieved from UniProt ( www.uniprot.org ).

    Article Title: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif
    Article Snippet: Separation was performed with a binary gradient from 5 to 32% B for 90 min with a total runtime of 2 h per sample on a nano-HPLC system (Ultimate 3000 nano-RSLC, Thermo) operated in a two-column setup (Acclaim PepMap 100 C18, ID 75 µm, trap column length 2 cm, particle size 3 µm, analytical column length 15 cm, particle size 2 µm, Thermo) coupled online to a high-resolution Q-TOF mass spectrometer (ImpactII, Bruker) as described by ref. . .. The Bruker HyStar Software (v3.2) was used to acquire line-mode MS spectra in a mass range from 200 to 1750 m/z at an acquisition rate of 4 Hz and the Top17 most intense ions were selected for fragmentation.

    Produced:

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: Paragraph title: Identification of the Exoprotease Produced Under Starving Conditions ... Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm).

    Fractionation:

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes
    Article Snippet: Nano-HPLC/Nano-ESI-LTQ-Orbitrap-MS/MS Fractionation of peptide mixtures was carried out on an Ultimate nano-HPLC system (Thermo Fisher Scientific) using reversed phase C18 columns (precolumn: Acclaim PepMap, 300 µm • 5 mm, 5 µm, 100 Å, separation column: Acclaim PepMap, 75 µm • 250 mm, 3 µm, 100 Å, Thermo Fisher Scientific). .. The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific).

    Migration:

    Article Title: Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa
    Article Snippet: To determine the zone of migration of casein exoproteases, in parallel, SDS-PAGE using the same conditions was performed, except casein was added to the gel, and the bands of interest were cut with a scalpel in pieces of approximately 1 mm3 , and thoroughly washed with (i) 25 mM ammonium bicarbonate (ABC), 50% acetonitrile (ACN); (ii) 100% ACN; (iii) 50 mM ABC; and (iv) 100% ACN. .. Just before injection to the nano HPLC system, samples were reconstituted in 15 μL of 0.1% FA, 5% ACN of which 3 μL were loaded into a Thermo UltiMate 3000 HPLC system using a pre-column/peptide trap Acclaim PepMap 100 C18 (300 μm × 1.5 cm), and a separation column Acclaim PepMap RSLC C18 (75 μm × 15 cm).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
    Article Snippet: Paragraph title: LC–MS ... The samples then underwent shotgun proteomic analysis on a nano-HPLC system (NanoLC 2D; Eksigent, Dublin, CA) coupled to a hybrid mass spectrometer (Orbitrap Velos Pro; Thermo-Electron, Bremen, Germany).

    Article Title: Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens
    Article Snippet: Paragraph title: Protein Identity Determination by Liquid Chromatography-Mass Spectrometry (LC-MS) ... The resulting peptide solution was separated using the Dionex Ultimate 3,000 nano-HPLC system (ThermoFischer Scientific, USA) and then analyzed using a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFischer Scientific, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 79
    Thermo Fisher high resolution nano hplc tandem mass spectrometry analysis
    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to <t>nano-HPLC</t> tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.
    High Resolution Nano Hplc Tandem Mass Spectrometry Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution nano hplc tandem mass spectrometry analysis/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high resolution nano hplc tandem mass spectrometry analysis - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    89
    Thermo Fisher ultimate nano hplc system
    <t>Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS</t> analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Ultimate Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate nano hplc system/product/Thermo Fisher
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ultimate nano hplc system - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    99
    Thermo Fisher nano hplc
    Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a <t>nano-HPLC.</t> The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.
    Nano Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc/product/Thermo Fisher
    Average 99 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
    nano hplc - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher nano hplc system
    <t>Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS</t> analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc system/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nano hplc system - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Journal: Scientific Reports

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans

    doi: 10.1038/srep36832

    Figure Lengend Snippet: Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Article Snippet: High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm).

    Techniques: Infection, High Performance Liquid Chromatography, Mass Spectrometry

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Journal: PLoS ONE

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    doi: 10.1371/journal.pone.0112886

    Figure Lengend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Article Snippet: Nano-HPLC/Nano-ESI-LTQ-Orbitrap-MS/MS Fractionation of peptide mixtures was carried out on an Ultimate nano-HPLC system (Thermo Fisher Scientific) using reversed phase C18 columns (precolumn: Acclaim PepMap, 300 µm • 5 mm, 5 µm, 100 Å, separation column: Acclaim PepMap, 75 µm • 250 mm, 3 µm, 100 Å, Thermo Fisher Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay

    Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a nano-HPLC. The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.

    Journal: Frontiers in Microbiology

    Article Title: Relative Quantitative Proteomic Analysis of Brucella abortus Reveals Metabolic Adaptation to Multiple Environmental Stresses

    doi: 10.3389/fmicb.2017.02347

    Figure Lengend Snippet: Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a nano-HPLC. The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.

    Article Snippet: Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) All experiments were performed on an LTQ Q-Exactive HF mass spectrometer (Thermo Scientific, USA) coupled online with a nano-HPLC (Ultimate 3000, Thermo Scientific) (Scheltema et al., ).

    Techniques: Mass Spectrometry, High Performance Liquid Chromatography

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Journal: PLoS ONE

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    doi: 10.1371/journal.pone.0112886

    Figure Lengend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Article Snippet: The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay