nano high pressure liquid chromatography system  (Thermo Fisher)


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    Thermo Fisher nano high pressure liquid chromatography system
    Nano High Pressure Liquid Chromatography System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano high pressure liquid chromatography system/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    nano high pressure liquid chromatography system - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites
    Article Snippet: .. HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany). ..

    Article Title: Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis
    Article Snippet: .. The microtitre plate was removed from the Triversa Nanomate and placed in the autosampler of the HPLC system (Ultimate 3000; Dionex, Thermo Fisher Scientific, Loughborough, UK), which is coupled to a Thermo Fisher Orbitrap Velos ETD mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via the Triversa Nanomate. .. Five μL aliquots were injected onto a Pepmap 100, C18 100 μm nanoviper trap (Thermo Fisher Scientific, Loughborough UK) for online desalting.

    Article Title: Adenosine diphosphate restricts the protein remodeling activity of the Hsp104 chaperone to Hsp70 assisted disaggregation
    Article Snippet: .. ADP concentration measurements Samples of the GFP renaturation reaction mixture (containing 1 μM Hsp104 D484K and optionally 2 μM Ssa1 and 0.5 μM Ydj1) were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) according to ( ) on GBC 1150 HPLC Pump, Spectra System AS3000 autosampler, Thermo Finnigan Spectra System UV6000LP. .. The separation was performed on 50 x 4,6 mm HyperClone 3u BDS C18, 130 A column (Phenomenex).

    Article Title: Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells
    Article Snippet: .. HPLC-PDA Analysis For the HPLC analysis of the extracts, a Thermo Finnigan® HPLC-PDA System (P4000 Pump, AS3000 Autosampler, PDA Detector UV8000, ChromQuest™ 4.2 Software) and a Supelco® RP18 Discovery HS-C18 (250 mm, 4.6 mm, and 5 μ m) column were employed. ..

    Article Title: Polyamines in biological samples: Rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography-tandem mass spectrometry
    Article Snippet: .. 2.4 Chromatographic methods All experiments were carried out on an Ultimate 3000 HPLC system comprising an autosampler and a 10-way switching valve unit coupled to a triple-quadrupole mass spectrometer, a Quantum TSQ Ultra AM, both from Thermo Fisher Scientific (San Jose, CA). .. The system was controlled by Xcalibur Software 2.1.

    Concentration Assay:

    Article Title: Adenosine diphosphate restricts the protein remodeling activity of the Hsp104 chaperone to Hsp70 assisted disaggregation
    Article Snippet: .. ADP concentration measurements Samples of the GFP renaturation reaction mixture (containing 1 μM Hsp104 D484K and optionally 2 μM Ssa1 and 0.5 μM Ydj1) were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) according to ( ) on GBC 1150 HPLC Pump, Spectra System AS3000 autosampler, Thermo Finnigan Spectra System UV6000LP. .. The separation was performed on 50 x 4,6 mm HyperClone 3u BDS C18, 130 A column (Phenomenex).

    Purification:

    Article Title: Off-Line Multidimensional Liquid Chromatography and Auto Sampling Result in Sample Loss in LC/LC–MS/MS
    Article Snippet: .. Fractions were then loaded into a 2.5 RP resin column (offline MudPIT) or purified by stage tip and placed in autosampler vials (offline MudPIT with autosampler [EASY-nLC II, Thermo]). .. Both MudPIT and analytical columns were assembled using a zero-dead volume union (Upchurch Scientific, Oak Harbor, WA).

    Mass Spectrometry:

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites
    Article Snippet: .. HPLC-FTMS The HPLC-FTMS system used an HPLC system (Accela LC with Accela Pump 60057-60010 and Accela Autosampler 60057-60020, Thermo Scientific, Dreieich, Germany) coupled to a Fourier transform mass spectrometer with a heated ESI source (LTQ Orbitrap XL, Thermo Scientific, Dreieich, Germany). ..

    Article Title: Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis
    Article Snippet: .. The microtitre plate was removed from the Triversa Nanomate and placed in the autosampler of the HPLC system (Ultimate 3000; Dionex, Thermo Fisher Scientific, Loughborough, UK), which is coupled to a Thermo Fisher Orbitrap Velos ETD mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via the Triversa Nanomate. .. Five μL aliquots were injected onto a Pepmap 100, C18 100 μm nanoviper trap (Thermo Fisher Scientific, Loughborough UK) for online desalting.

    Article Title: Polyamines in biological samples: Rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography-tandem mass spectrometry
    Article Snippet: .. 2.4 Chromatographic methods All experiments were carried out on an Ultimate 3000 HPLC system comprising an autosampler and a 10-way switching valve unit coupled to a triple-quadrupole mass spectrometer, a Quantum TSQ Ultra AM, both from Thermo Fisher Scientific (San Jose, CA). .. The system was controlled by Xcalibur Software 2.1.

    Software:

    Article Title: Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells
    Article Snippet: .. HPLC-PDA Analysis For the HPLC analysis of the extracts, a Thermo Finnigan® HPLC-PDA System (P4000 Pump, AS3000 Autosampler, PDA Detector UV8000, ChromQuest™ 4.2 Software) and a Supelco® RP18 Discovery HS-C18 (250 mm, 4.6 mm, and 5 μ m) column were employed. ..

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    Thermo Fisher high resolution nano hplc tandem mass spectrometry analysis
    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to <t>nano-HPLC</t> tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.
    High Resolution Nano Hplc Tandem Mass Spectrometry Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution nano hplc tandem mass spectrometry analysis/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high resolution nano hplc tandem mass spectrometry analysis - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    88
    Thermo Fisher ultimate nano hplc system
    <t>Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS</t> analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Ultimate Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate nano hplc system/product/Thermo Fisher
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    ultimate nano hplc system - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    Thermo Fisher nano hplc
    Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a <t>nano-HPLC.</t> The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.
    Nano Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc/product/Thermo Fisher
    Average 92 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    nano hplc - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Journal: Scientific Reports

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans

    doi: 10.1038/srep36832

    Figure Lengend Snippet: Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Article Snippet: High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm).

    Techniques: Infection, High Performance Liquid Chromatography, Mass Spectrometry

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Journal: PLoS ONE

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    doi: 10.1371/journal.pone.0112886

    Figure Lengend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Article Snippet: Nano-HPLC/Nano-ESI-LTQ-Orbitrap-MS/MS Fractionation of peptide mixtures was carried out on an Ultimate nano-HPLC system (Thermo Fisher Scientific) using reversed phase C18 columns (precolumn: Acclaim PepMap, 300 µm • 5 mm, 5 µm, 100 Å, separation column: Acclaim PepMap, 75 µm • 250 mm, 3 µm, 100 Å, Thermo Fisher Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay

    Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a nano-HPLC. The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.

    Journal: Frontiers in Microbiology

    Article Title: Relative Quantitative Proteomic Analysis of Brucella abortus Reveals Metabolic Adaptation to Multiple Environmental Stresses

    doi: 10.3389/fmicb.2017.02347

    Figure Lengend Snippet: Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a nano-HPLC. The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.

    Article Snippet: Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) All experiments were performed on an LTQ Q-Exactive HF mass spectrometer (Thermo Scientific, USA) coupled online with a nano-HPLC (Ultimate 3000, Thermo Scientific) (Scheltema et al., ).

    Techniques: Mass Spectrometry, High Performance Liquid Chromatography

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Journal: PLoS ONE

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    doi: 10.1371/journal.pone.0112886

    Figure Lengend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Article Snippet: The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay