nano high pressure liquid chromatography system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nano high pressure liquid chromatography system
    Nano High Pressure Liquid Chromatography System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano high pressure liquid chromatography system/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    nano high pressure liquid chromatography system - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: NGF-induced TrkA/CD44 association is involved in tumor aggressiveness and resistance to lestaurtinib
    Article Snippet: .. Nano-LC-MS/MS Q-Star analysis Peptidic digests were extracted from the 1-D gel band and nanoLC-nanoESI-MS/MS analyses were performed on an hybrid quadrupole time-of-flight mass spectrometer (Q-Star, Applied Biosystems, France) equipped with a nano-electrospray ion source coupled with a nano high pressure liquid chromatography system (LC Packings Dionex, France) as previously described [ ]. .. Western blot Whole cell lysates or immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes (Westran® Clear Signal).

    Article Title: Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein *
    Article Snippet: .. A nanoLC-nanoESI/SIM-MS system was used for the detection of proNGF protein, as described previously , with an ion trap mass spectrometer (LCQ Deca XP+ ; Thermo Fisher) equipped with a nanoelectrospray ion source coupled with a nano high pressure liquid chromatography system (LC Packings, Dionex). ..

    Flow Cytometry:

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure ▿ Strain Newman Alters the Response to Biocide Exposure ▿ †
    Article Snippet: A 25-μl portion of each sample was analyzed with a nano-high-pressure liquid chromatography system (Ultimate; Dionex, The Netherlands; equipped with a Famos autosampler) coupled to an ESI QTOF hybrid mass spectrometer (Allied Biosystems). .. Samples were loaded on a C18 trapping column (Inertsil; LC Packings) with a flow rate of 20 μl/min 0.1% TFA.

    Article Title: The Cellular Protein Lyric Interacts with HIV-1 Gag ▿The Cellular Protein Lyric Interacts with HIV-1 Gag ▿ †
    Article Snippet: Twenty-five microliters of the sample was analyzed by a nano-high-pressure liquid chromatography system (Dionex, Amsterdam, Netherlands) coupled to an electron spray ionization (ESI) LTQ Orbitrap mass spectrometer (Thermo Fisher). .. The samples were loaded on a C18 trapping column (Inertsil; LC Packings, Amsterdam, Netherlands) with a flow rate of 10 μl/min 0.1% TFA.

    Article Title: Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina
    Article Snippet: Eight microliter of each sample was injected by autosampler and concentrated on a trapping column (Pepmap100, C18, 100 μm × 2 cm, 5 μm, Thermo Fisher Scientific) with water containing 0.1 % formic acid and 2 % ACN at a flow rate of 4 μl/min. .. First from 4 to 12 % over 5 min, then from 12 to 30 % over 30 min followed by a final step gradient to 90 % solvent B, which was maintained for 5 min using a nano-high-pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: Each sample of 8 μL was injected using an autosampler and concentrated on a trapping column (Pepmap100, C18 , 100 μm × 2 cm, 5 μm, Thermo Fisher Scientific) with water containing 0.1% formic acid and 2% acetonitrile at flow rates of 4 μL min− 1 . .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: The histone methyltransferase SUV420H2 and Heterochromatin Proteins HP1 interact but show different dynamic behaviours
    Article Snippet: Each tryptic digest sample was subjected to nano-LC-nano-ESI-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. The samples were first desalted and then concentrated on a reverse phase precolumn of 5 mm × 0.3 mm inner diameter (Dionex) by solvent A (95% H2 O, 5% acetonitrile, 0.1% HCOOH) delivered by the Switchos pumping device (LC Packings Dionex) at a flow rate of 10 μl/min for 3 min. Peptides were separated on a 15 cm × 75 μm-inner diameter C18 PepMap column (Dionex).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: Each sample of 8 μL was injected using an autosampler and concentrated on a trapping column (Pepmap100, C18 , 100 μm × 2 cm, 5 μm, Thermo Fisher Scientific) with water containing 0.1% formic acid and 2% acetonitrile at flow rates of 4 μL min−1 . .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Chromatography:

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure ▿ Strain Newman Alters the Response to Biocide Exposure ▿ †
    Article Snippet: Paragraph title: In-gel tryptic digestion and liquid chromatography-tandem mass spectrometry (MS/MS) analysis. ... A 25-μl portion of each sample was analyzed with a nano-high-pressure liquid chromatography system (Ultimate; Dionex, The Netherlands; equipped with a Famos autosampler) coupled to an ESI QTOF hybrid mass spectrometer (Allied Biosystems).

    Article Title: Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina
    Article Snippet: Chromatography was performed with 0.1 % formic acid in solvent A (100 % water) and B (100 % acetonitrile). .. First from 4 to 12 % over 5 min, then from 12 to 30 % over 30 min followed by a final step gradient to 90 % solvent B, which was maintained for 5 min using a nano-high-pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: Chromatography was performed using 0.1% formic acid in solvent A (100% water) and B (100% acetonitrile). .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: Chromatography was performed using 0.1% formic acid in solvent A (100% water) and B (100% acetonitrile). .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Protein Extraction:

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: Paragraph title: Protein Extraction, Mass Spectrometry and Data Analysis ... The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: Paragraph title: Protein extraction and mass spectrometry and data analysis ... The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Centrifugation:

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: The obtained peptides were purified and desalted using C18 StageTips ( , ), dried using vacuum centrifugation, and prior to LC-MS/MS measurements, they were reconstituted in 20 μL 0.1% trifluoroacetic acid/2% acetonitrile. .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: The obtained peptides were purified and desalted using C18 StageTips , dried using vacuum centrifugation, and prior to LC-MS/MS measurements, they were reconstituted in 20 μL 0.1% trifluoroacetic acid/2% acetonitrile. .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Purification:

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: The obtained peptides were purified and desalted using C18 StageTips ( , ), dried using vacuum centrifugation, and prior to LC-MS/MS measurements, they were reconstituted in 20 μL 0.1% trifluoroacetic acid/2% acetonitrile. .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: The obtained peptides were purified and desalted using C18 StageTips , dried using vacuum centrifugation, and prior to LC-MS/MS measurements, they were reconstituted in 20 μL 0.1% trifluoroacetic acid/2% acetonitrile. .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Concentration Assay:

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: The modified bicelles were mixed with R1–3 to reach a final concentration of 50 mM of lipids and 15 μ M of protein. .. The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific).

    Incubation:

    Article Title: The histone methyltransferase SUV420H2 and Heterochromatin Proteins HP1 interact but show different dynamic behaviours
    Article Snippet: The supernatant was replaced by 50 μl of 25 mM NH4 HCO3 , and the samples were incubated overnight at 37°C. .. Each tryptic digest sample was subjected to nano-LC-nano-ESI-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex).

    Article Title: Interaction Proteomics Analysis of Polycomb Proteins Defines Distinct PRC1 Complexes in Mammalian Cells *
    Article Snippet: The supernatant was replaced by 50 μl of 25 m m NH4 HCO3 , and the samples were incubated overnight at 37 °C. .. Each tryptic digest sample was subjected to nano-LC-nano-electrospray ionization (ESI)-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex).

    Mass Spectrometry:

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure ▿ Strain Newman Alters the Response to Biocide Exposure ▿ †
    Article Snippet: .. A 25-μl portion of each sample was analyzed with a nano-high-pressure liquid chromatography system (Ultimate; Dionex, The Netherlands; equipped with a Famos autosampler) coupled to an ESI QTOF hybrid mass spectrometer (Allied Biosystems). .. Samples were loaded on a C18 trapping column (Inertsil; LC Packings) with a flow rate of 20 μl/min 0.1% TFA.

    Article Title: The Cellular Protein Lyric Interacts with HIV-1 Gag ▿The Cellular Protein Lyric Interacts with HIV-1 Gag ▿ †
    Article Snippet: .. Twenty-five microliters of the sample was analyzed by a nano-high-pressure liquid chromatography system (Dionex, Amsterdam, Netherlands) coupled to an electron spray ionization (ESI) LTQ Orbitrap mass spectrometer (Thermo Fisher). .. The samples were loaded on a C18 trapping column (Inertsil; LC Packings, Amsterdam, Netherlands) with a flow rate of 10 μl/min 0.1% TFA.

    Article Title: Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina
    Article Snippet: First from 4 to 12 % over 5 min, then from 12 to 30 % over 30 min followed by a final step gradient to 90 % solvent B, which was maintained for 5 min using a nano-high-pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific). .. Ionized peptides were measured and fragmented by a Q Exactive mass spectrometer (Thermo Fisher Scientific).

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: Paragraph title: Protein Extraction, Mass Spectrometry and Data Analysis ... The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: The histone methyltransferase SUV420H2 and Heterochromatin Proteins HP1 interact but show different dynamic behaviours
    Article Snippet: .. Each tryptic digest sample was subjected to nano-LC-nano-ESI-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. Samples were resuspended in 3 μl of 0.1% HCOOH, and 1.4 μl were injected into the mass spectrometer using a Famos autosampler (LC Packings Dionex).

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: Paragraph title: Click chemistry and liquid chromatography coupled with MS ... The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific).

    Article Title: NGF-induced TrkA/CD44 association is involved in tumor aggressiveness and resistance to lestaurtinib
    Article Snippet: .. Nano-LC-MS/MS Q-Star analysis Peptidic digests were extracted from the 1-D gel band and nanoLC-nanoESI-MS/MS analyses were performed on an hybrid quadrupole time-of-flight mass spectrometer (Q-Star, Applied Biosystems, France) equipped with a nano-electrospray ion source coupled with a nano high pressure liquid chromatography system (LC Packings Dionex, France) as previously described [ ]. .. Western blot Whole cell lysates or immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes (Westran® Clear Signal).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: Paragraph title: Protein extraction and mass spectrometry and data analysis ... The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Article Title: Interaction Proteomics Analysis of Polycomb Proteins Defines Distinct PRC1 Complexes in Mammalian Cells *
    Article Snippet: .. Each tryptic digest sample was subjected to nano-LC-nano-electrospray ionization (ESI)-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. Samples were resuspended in 3 μl of 0.1% HCOOH, and 1.4 μl were injected into the mass spectrometer using a Famos autosampler (LC Packings Dionex).

    Article Title: Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein *
    Article Snippet: .. A nanoLC-nanoESI/SIM-MS system was used for the detection of proNGF protein, as described previously , with an ion trap mass spectrometer (LCQ Deca XP+ ; Thermo Fisher) equipped with a nanoelectrospray ion source coupled with a nano high pressure liquid chromatography system (LC Packings, Dionex). ..

    Tandem Mass Spectroscopy:

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure ▿ Strain Newman Alters the Response to Biocide Exposure ▿ †
    Article Snippet: Paragraph title: In-gel tryptic digestion and liquid chromatography-tandem mass spectrometry (MS/MS) analysis. ... A 25-μl portion of each sample was analyzed with a nano-high-pressure liquid chromatography system (Ultimate; Dionex, The Netherlands; equipped with a Famos autosampler) coupled to an ESI QTOF hybrid mass spectrometer (Allied Biosystems).

    Article Title: Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina
    Article Snippet: First from 4 to 12 % over 5 min, then from 12 to 30 % over 30 min followed by a final step gradient to 90 % solvent B, which was maintained for 5 min using a nano-high-pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific). .. For an unbiased analysis, continuous scanning of eluted peptide ions was carried out between 400 and 12,000 m /z , automatically switching to MS/MS higher energy collisional dissociation (HCD) mode and 12 MS/MS events per survey scan.

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific). .. For an unbiased analysis, continuous scanning of the eluted peptide ions was performed between 400 and 1200 m /z and then automatically switched to MS/MS higher energy collisional dissociation mode with twelve MS/MS events per survey scan.

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific). .. Peptides were directly eluted from the nano-high pressure liquid chromatography column to the nanoelectrospray ion source of an LTQ-Orbitrap XL (ThermoScientific) mass spectrometer operating in data-dependent mode by automatically switching between full MS scan and MS/MS acquisitions on the 15 most intense precursor ions, as described previously ( ).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific). .. For an unbiased analysis, continuous scanning of the eluted peptide ions was performed between 400–1200 m /z and then automatically switched to MS/MS higher energy collisional dissociation mode with twelve MS/MS events per survey scan.

    Modification:

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: The modified bicelles were mixed with R1–3 to reach a final concentration of 50 mM of lipids and 15 μ M of protein. .. The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific).

    Staining:

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: The staining was performed using Coomassie blue (InstantBlue, Expedeon). .. The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific).

    Injection:

    Article Title: Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina
    Article Snippet: Eight microliter of each sample was injected by autosampler and concentrated on a trapping column (Pepmap100, C18, 100 μm × 2 cm, 5 μm, Thermo Fisher Scientific) with water containing 0.1 % formic acid and 2 % ACN at a flow rate of 4 μl/min. .. First from 4 to 12 % over 5 min, then from 12 to 30 % over 30 min followed by a final step gradient to 90 % solvent B, which was maintained for 5 min using a nano-high-pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: Each sample of 8 μL was injected using an autosampler and concentrated on a trapping column (Pepmap100, C18 , 100 μm × 2 cm, 5 μm, Thermo Fisher Scientific) with water containing 0.1% formic acid and 2% acetonitrile at flow rates of 4 μL min− 1 . .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific).

    Article Title: The histone methyltransferase SUV420H2 and Heterochromatin Proteins HP1 interact but show different dynamic behaviours
    Article Snippet: Each tryptic digest sample was subjected to nano-LC-nano-ESI-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. Samples were resuspended in 3 μl of 0.1% HCOOH, and 1.4 μl were injected into the mass spectrometer using a Famos autosampler (LC Packings Dionex).

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: Each sample of 8 μL was injected using an autosampler and concentrated on a trapping column (Pepmap100, C18 , 100 μm × 2 cm, 5 μm, Thermo Fisher Scientific) with water containing 0.1% formic acid and 2% acetonitrile at flow rates of 4 μL min−1 . .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific).

    Article Title: Interaction Proteomics Analysis of Polycomb Proteins Defines Distinct PRC1 Complexes in Mammalian Cells *
    Article Snippet: Each tryptic digest sample was subjected to nano-LC-nano-electrospray ionization (ESI)-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. Samples were resuspended in 3 μl of 0.1% HCOOH, and 1.4 μl were injected into the mass spectrometer using a Famos autosampler (LC Packings Dionex).

    Liquid Chromatography:

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure ▿ Strain Newman Alters the Response to Biocide Exposure ▿ †
    Article Snippet: .. A 25-μl portion of each sample was analyzed with a nano-high-pressure liquid chromatography system (Ultimate; Dionex, The Netherlands; equipped with a Famos autosampler) coupled to an ESI QTOF hybrid mass spectrometer (Allied Biosystems). .. Samples were loaded on a C18 trapping column (Inertsil; LC Packings) with a flow rate of 20 μl/min 0.1% TFA.

    Article Title: The Cellular Protein Lyric Interacts with HIV-1 Gag ▿The Cellular Protein Lyric Interacts with HIV-1 Gag ▿ †
    Article Snippet: .. Twenty-five microliters of the sample was analyzed by a nano-high-pressure liquid chromatography system (Dionex, Amsterdam, Netherlands) coupled to an electron spray ionization (ESI) LTQ Orbitrap mass spectrometer (Thermo Fisher). .. The samples were loaded on a C18 trapping column (Inertsil; LC Packings, Amsterdam, Netherlands) with a flow rate of 10 μl/min 0.1% TFA.

    Article Title: Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina
    Article Snippet: .. First from 4 to 12 % over 5 min, then from 12 to 30 % over 30 min followed by a final step gradient to 90 % solvent B, which was maintained for 5 min using a nano-high-pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific). .. Ionized peptides were measured and fragmented by a Q Exactive mass spectrometer (Thermo Fisher Scientific).

    Article Title: Gut Bacteria Metabolism Impacts Immune Recovery in HIV-infected Individuals
    Article Snippet: .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate3000 UHPLC, Thermo Fisher Scientific). .. Ionized peptides were measured and fragmented using a Q Exactive mass spectrometer (Thermo Fisher Scientific).

    Article Title: The histone methyltransferase SUV420H2 and Heterochromatin Proteins HP1 interact but show different dynamic behaviours
    Article Snippet: .. Each tryptic digest sample was subjected to nano-LC-nano-ESI-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. Samples were resuspended in 3 μl of 0.1% HCOOH, and 1.4 μl were injected into the mass spectrometer using a Famos autosampler (LC Packings Dionex).

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: .. The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific). ..

    Article Title: HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases
    Article Snippet: .. The solvent B gradient was set from 4 to 8% for the first 15 min and subsequently increased to 20% for the next 110 min. After this, solvent B was increased from 20% to 30% over 15 min, from 30% to 40% over 10 min, and finally switched to 90% solvent B for an additional 10 min using a nano-high pressure liquid chromatography system (Ultimate 3000 UHPLC, Thermo Fisher Scientific). .. Ionized peptides were measured and fragmented using a Q Exactive mass spectrometer (Thermo Fisher Scientific).

    Article Title: Interaction Proteomics Analysis of Polycomb Proteins Defines Distinct PRC1 Complexes in Mammalian Cells *
    Article Snippet: .. Each tryptic digest sample was subjected to nano-LC-nano-electrospray ionization (ESI)-MS/MS analysis on an ion trap mass spectrometer (LCQ Deca XP+ , Thermo Electron Corp.), equipped with a nanoelectrospray ion source, coupled with a nano-high pressure liquid chromatography system (LC Packings Dionex). .. Samples were resuspended in 3 μl of 0.1% HCOOH, and 1.4 μl were injected into the mass spectrometer using a Famos autosampler (LC Packings Dionex).

    SDS Page:

    Article Title: The Cellular Protein Lyric Interacts with HIV-1 Gag ▿The Cellular Protein Lyric Interacts with HIV-1 Gag ▿ †
    Article Snippet: For TAP- and GFP-based screens, proteins were separated by SDS-PAGE and the whole lane was cut in pieces of about 4 mm in length. .. Twenty-five microliters of the sample was analyzed by a nano-high-pressure liquid chromatography system (Dionex, Amsterdam, Netherlands) coupled to an electron spray ionization (ESI) LTQ Orbitrap mass spectrometer (Thermo Fisher).

    Article Title: Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids
    Article Snippet: Then, the protein/lipid complexes were exposed to ultraviolet A radiation (5 × 15 W, Stratalinker; GE Healthcare) for 5 min just before SDS-PAGE. .. The peptide extract was loaded (10 μ L) on a nano-high pressure liquid chromatography system (LC Packings Ultimate 3000, Dionex, Sunnyvale, CA) equipped with a trapping precolumn (5 mm × 300 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific, Waltham, MA) and an analytical column (15 cm × 75 μ m internal diameter, 300 Å pore size, Pepmap C18, 5 μ m, ThermoScientific).

    Software:

    Article Title: Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein *
    Article Snippet: A nanoLC-nanoESI/SIM-MS system was used for the detection of proNGF protein, as described previously , with an ion trap mass spectrometer (LCQ Deca XP+ ; Thermo Fisher) equipped with a nanoelectrospray ion source coupled with a nano high pressure liquid chromatography system (LC Packings, Dionex). .. The deconvoluted mass spectrum was depicted with the Bioworks 3.1 software (Thermo Fisher).

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  • 78
    Thermo Fisher high resolution nano hplc tandem mass spectrometry analysis
    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to <t>nano-HPLC</t> tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.
    High Resolution Nano Hplc Tandem Mass Spectrometry Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution nano hplc tandem mass spectrometry analysis/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high resolution nano hplc tandem mass spectrometry analysis - by Bioz Stars, 2020-02
    78/100 stars
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    84
    Thermo Fisher ultimate nano hplc system
    <t>Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS</t> analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Ultimate Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate nano hplc system/product/Thermo Fisher
    Average 84 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ultimate nano hplc system - by Bioz Stars, 2020-02
    84/100 stars
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    99
    Thermo Fisher nano hplc
    Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a <t>nano-HPLC.</t> The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.
    Nano Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc/product/Thermo Fisher
    Average 99 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
    nano hplc - by Bioz Stars, 2020-02
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    92
    Thermo Fisher nano hplc system
    <t>Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS</t> analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.
    Nano Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano hplc system/product/Thermo Fisher
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nano hplc system - by Bioz Stars, 2020-02
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    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Journal: Scientific Reports

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans

    doi: 10.1038/srep36832

    Figure Lengend Snippet: Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Article Snippet: High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm).

    Techniques: Infection, High Performance Liquid Chromatography, Mass Spectrometry

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Journal: PLoS ONE

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    doi: 10.1371/journal.pone.0112886

    Figure Lengend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Article Snippet: Nano-HPLC/Nano-ESI-LTQ-Orbitrap-MS/MS Fractionation of peptide mixtures was carried out on an Ultimate nano-HPLC system (Thermo Fisher Scientific) using reversed phase C18 columns (precolumn: Acclaim PepMap, 300 µm • 5 mm, 5 µm, 100 Å, separation column: Acclaim PepMap, 75 µm • 250 mm, 3 µm, 100 Å, Thermo Fisher Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay

    Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a nano-HPLC. The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.

    Journal: Frontiers in Microbiology

    Article Title: Relative Quantitative Proteomic Analysis of Brucella abortus Reveals Metabolic Adaptation to Multiple Environmental Stresses

    doi: 10.3389/fmicb.2017.02347

    Figure Lengend Snippet: Experimental design of the quantitative proteomics of Brucella abortus under environmental stress. A label-free relative quantitative proteomics approach was utilized to investigate and compare the global proteomic changes of B. abortus in response to a variety of distinct stresses including a control condition, seven single-stress conditions, and a multi-stress condition. Protein samples were prepared and proteolytic-digested using trypsin enzymes. Peptides were analyzed on a Q-Exactive HF MS coupled online with a nano-HPLC. The identified proteins were quantified using a label-free approach and further functionally analyzed using the COG and KEGG databases.

    Article Snippet: Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) All experiments were performed on an LTQ Q-Exactive HF mass spectrometer (Thermo Scientific, USA) coupled online with a nano-HPLC (Ultimate 3000, Thermo Scientific) (Scheltema et al., ).

    Techniques: Mass Spectrometry, High Performance Liquid Chromatography

    Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Journal: PLoS ONE

    Article Title: Analysis of Nidogen-1/Laminin γ1 Interaction by Cross-Linking, Mass Spectrometry, and Computational Modeling Reveals Multiple Binding Modes

    doi: 10.1371/journal.pone.0112886

    Figure Lengend Snippet: Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of cross-linked peptides derived from nidogen-1 G2 and G3 domain. The cross-linked product comprises amino acids 407–420 of the G2 domain (α-peptide, red) and 939–949 of the G3 domain (β-peptide, green), in which K-407 is connected to K-948/949.

    Article Snippet: The nano-HPLC system was directly coupled to the nano-ESI source (Proxeon) of an LTQ-Orbitrap XL hybrid mass spectrometer (Thermo Fisher Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay