Journal: PLoS ONE
Article Title: Functionally Deregulated AML1/RUNX1 Cooperates with BCR-ABL to Induce a Blastic Phase-Like Phenotype of Chronic Myelogenous Leukemia in Mice
doi: 10.1371/journal.pone.0074864
Figure Lengend Snippet: (A) Quantitative RT-PCR of AML1 transcripts in CML cell lines infected with shRNA viruses for luciferase (control) and AML1 (shRNA-AML1–1 and shRNA-AML1–2). Two different viruses were used for AML1 silencing and effectively silenced AML1 expression. Results are normalized on the basis of β-actin transcript levels, and are presented as relative values to those of the control. (B) Growth curves of MegA2, MegO1 and Nalm1 cell lines infected with shRNA viruses for luciferase and AML1 . Viable cells were counted every other day with the use of trypan blue.
Article Snippet: CML cell lines used were as follows: K562 (ATCC-CLL-243), Nalm1 (ATCC-CRL-1567), BV-173(DSMZ ACC-20), MegO1 (provided by Dr. Michinori Ogura, Nagoya University), and MegA2 (provided by Dr. Akihiro Abe, Fujita Health University).
Techniques: Quantitative RT-PCR, Infection, shRNA, Luciferase, Expressing