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Santa Cruz Biotechnology na3 vo4
Na3 Vo4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 21 article reviews
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na3 vo4 - by Bioz Stars, 2020-07
93/100 stars

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Homogenization:

Article Title: DIFFERENTIAL POSTPARTUM SENSITIVITY TO THE ANXIETY-MODULATING EFFECTS OF OFFSPRING CONTACT IS ASSOCIATED WITH INNATE ANXIETY AND BRAINSTEM LEVELS OF DOPAMINE BETA-HYDROXYLASE IN FEMALE LABORATORY RATS
Article Snippet: .. For homogenization, the brainstem was placed in a solution of 1 mL of RIPA, 10 μL Na3 VO4 , 10μL PMSF, and 10 μL protease inhibitor (SC-24948, all Santa Cruz Biotechnology, Santa Cruz, CA, USA) and homogenized on ice with pulses of a sonic dismembrator (Fisher Scientific, Pittsburgh, PA, USA) for 20 s at 100% amplitude. ..

Protease Inhibitor:

Article Title: DIFFERENTIAL POSTPARTUM SENSITIVITY TO THE ANXIETY-MODULATING EFFECTS OF OFFSPRING CONTACT IS ASSOCIATED WITH INNATE ANXIETY AND BRAINSTEM LEVELS OF DOPAMINE BETA-HYDROXYLASE IN FEMALE LABORATORY RATS
Article Snippet: .. For homogenization, the brainstem was placed in a solution of 1 mL of RIPA, 10 μL Na3 VO4 , 10μL PMSF, and 10 μL protease inhibitor (SC-24948, all Santa Cruz Biotechnology, Santa Cruz, CA, USA) and homogenized on ice with pulses of a sonic dismembrator (Fisher Scientific, Pittsburgh, PA, USA) for 20 s at 100% amplitude. ..

Article Title: Sustained Radiosensitization of Hypoxic Glioma Cells after Oxygen Pretreatment in an Animal Model of Glioblastoma and In Vitro Models of Tumor Hypoxia
Article Snippet: .. Nuclear lysates were prepared in buffer containing 20 mM HEPES (pH 7.9), 25% (v/v) glycerol, 0.4 M KCl, 1.5 mM MgCl2 , 0.2 M EDTA, supplemented with 10 mM Na3 VO4 , 5 mM NaFl, 5 mM DTT, 0.2 mM PMSF, and Complete Protease Inhibitor Cocktail (Santa Cruz). .. Protein concentrations were measured using Coomassie Plus Reagent (Thermo Scientific).

Article Title: Macroautophagy regulates nuclear NOTCH1 activity through multiple p62 binding sites
Article Snippet: .. Whole cell protein extraction was performed by lysing cell monolayers in a RIPA lysis buffer containing 1 mM PMSF, 1 mM Na3 VO4 and protease inhibitor cocktail (Santa Cruz Biotechnology). .. The nuclear and cytoplasmic protein was extracted using Nuclear Extract Kit (Active Motif).

Protein Extraction:

Article Title: Macroautophagy regulates nuclear NOTCH1 activity through multiple p62 binding sites
Article Snippet: .. Whole cell protein extraction was performed by lysing cell monolayers in a RIPA lysis buffer containing 1 mM PMSF, 1 mM Na3 VO4 and protease inhibitor cocktail (Santa Cruz Biotechnology). .. The nuclear and cytoplasmic protein was extracted using Nuclear Extract Kit (Active Motif).

Immunoprecipitation:

Article Title: Genomically Incorporated 5-Fluorouracil that Escapes UNG-Initiated Base Excision Repair Blocks DNA Replication and Activates Homologous Recombination
Article Snippet: .. To detect SMUG1, cells were lysed in 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 0.15 M NaCl, 0.01 M sodium phosphate (pH 7.2), 2 mM EDTA, 1 mM Na3 VO4 , 10 mM 2-glycerophosphate, 10 µ g/ml leupeptin, 5 µ g/ml aprotinin, 5 µ g/ml pepstatin, and 20 nM microcystin-LR, and equal amounts of protein were immunoprecipitated using 1 μ g anti-SMUG1 immunoglobulin G (Santa Cruz, N-19) and 15 μ l packed protein G Sepharose. .. Washed precipitates were separated by SDS-PAGE and immunoblotted with anti-SMUG1 IgG (Santa Cruz, H-11).

Incubation:

Article Title: The PAAD/PYRIN-Family Protein ASC Is a Dual Regulator of a Conserved Step in Nuclear Factor ?B Activation Pathways
Article Snippet: .. Immune-complexes were washed twice in lysis buffer (as above), once in lysis buffer containing 2 M urea followed by two washes in kinase buffer (20 mM Hepes [pH 7.6], 50 mM NaCl, 20 mM β-glycerophosphate, 1 mM Na3 VO4 , 0.5 mM DTT), equilibrated for 5 min in kinase buffer, adjusted to 10 mM MgCl2 and 1 mM DTT, and finally incubated in 20 μl kinase buffer supplemented with 35 μM ATP, 5 μCi γ[32 P] ATP and 1 μg glutathionine-S-transferase (GST)-IκBα (Santa Cruz Biotechnology, Inc.) at 30°C for 30 min ( ). .. NF-κB DNA-binding Activity Assays.

other:

Article Title: Microtubule sliding drives proplatelet elongation and is dependent on cytoplasmic dynein
Article Snippet: Methylcellulose (STEMCELL Technologies Inc., Vancouver, BC, Canada), Ciliobrevin D, erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA; Calbiochem, San Diego, CA), nocodazole, taxol (Sigma-Aldrich, St. Louis, MO), and Na3 VO4 (Santa Cruz Biotechnology, Dallas, TX) were used.

Lysis:

Article Title: Macroautophagy regulates nuclear NOTCH1 activity through multiple p62 binding sites
Article Snippet: .. Whole cell protein extraction was performed by lysing cell monolayers in a RIPA lysis buffer containing 1 mM PMSF, 1 mM Na3 VO4 and protease inhibitor cocktail (Santa Cruz Biotechnology). .. The nuclear and cytoplasmic protein was extracted using Nuclear Extract Kit (Active Motif).

Article Title: VCE‐004.3, a cannabidiol aminoquinone derivative, prevents bleomycin‐induced skin fibrosis and inflammation through PPARγ‐ and CB2 receptor‐dependent pathways
Article Snippet: .. After the various treatments, cells were washed with PBS and proteins extracted in 50 μL of lysis buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol and 1% NP‐40) with 10 mM NaF, 1 mM Na3 VO4 , 10 μg·mL−1 leupeptine, 1 μg·mL−1 pepstatin and aprotinin and 1 μL·mL−1 (1:500; sc‐7383, Santa Cruz Biotechnology, Dallas, TX, USA) and ERK 1/2 (1:2000; M5670, Sigma, St Louis, MO, USA) overnight at 4°C. .. Membranes were washed and incubated with the appropriate HRP‐conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).

Article Title: The PAAD/PYRIN-Family Protein ASC Is a Dual Regulator of a Conserved Step in Nuclear Factor ?B Activation Pathways
Article Snippet: .. Immune-complexes were washed twice in lysis buffer (as above), once in lysis buffer containing 2 M urea followed by two washes in kinase buffer (20 mM Hepes [pH 7.6], 50 mM NaCl, 20 mM β-glycerophosphate, 1 mM Na3 VO4 , 0.5 mM DTT), equilibrated for 5 min in kinase buffer, adjusted to 10 mM MgCl2 and 1 mM DTT, and finally incubated in 20 μl kinase buffer supplemented with 35 μM ATP, 5 μCi γ[32 P] ATP and 1 μg glutathionine-S-transferase (GST)-IκBα (Santa Cruz Biotechnology, Inc.) at 30°C for 30 min ( ). .. NF-κB DNA-binding Activity Assays.

Staining:

Article Title: Kinetics and extent of protein tyrosine kinase activation in individual T cells upon antigenic stimulation
Article Snippet: .. The cells were fixed with PBS containing 3% paraformaldehyde, 2 mm Na3 VO4, permeabilized with washing buffer and stained with an anti-PTyr antibody (Santa Cruz) and an anti-human immunoglobulin biotin-labelled antibody (SBA) in HEPES-buffered PBS containing 0·1% saponin, 5% BSA, 2 mm Na3 VO4, followed by a FITC-labelled goat anti-mouse antibody (SBA) and Texas Red-labelled streptavidin (SBA). .. The samples were mounted in 90% glycerol–PBS containing 2·5% 1-4-diazabicyclo (2.2.2) octane (DABCO, Fluka AG, Buchs, Switzerland) and were examined using a Carl Zeiss, LSM 410, confocal microscope (Carl Zeiss, Jena, Germany).

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  • 93
    Santa Cruz Biotechnology immunoprecipitation buffer
    PTP1B dephosphorylates endocytosed soma surface-derived Trk receptors (A) NGF-induced association of TrkA with PTP1B Y46F/D181A substrate trapping mutant, but not PTP1B WT , in sympathetic neurons. Adenoviruses under Tet-On regulation were used to express PTP1B WT -Myc or PTP1B Y46F/D181A -Myc in sympathetic neurons. No exogenous PTP1B-Myc expression is observed in absence of doxycycline (Dox). <t>Immunoprecipitation</t> was done with anti-Myc, and immunoblotting with anti-Myc or anti-TrkA antibodies. (B) NGF-induced tyrosine phosphorylation of TrkA is suppressed by PTP1B WT over-expression, and enhanced by PTP1B Y46F/D181A substrate-trapping mutant. (C) Densitometric quantification of P-TrkA levels normalized to total TrkA. **p
    Immunoprecipitation Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoprecipitation buffer/product/Santa Cruz Biotechnology
    Average 93 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    immunoprecipitation buffer - by Bioz Stars, 2020-07
    93/100 stars
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    92
    Santa Cruz Biotechnology inhibitors
    PTP1B dephosphorylates endocytosed soma surface-derived Trk receptors (A) NGF-induced association of TrkA with PTP1B Y46F/D181A substrate trapping mutant, but not PTP1B WT , in sympathetic neurons. Adenoviruses under Tet-On regulation were used to express PTP1B WT -Myc or PTP1B Y46F/D181A -Myc in sympathetic neurons. No exogenous PTP1B-Myc expression is observed in absence of doxycycline (Dox). <t>Immunoprecipitation</t> was done with anti-Myc, and immunoblotting with anti-Myc or anti-TrkA antibodies. (B) NGF-induced tyrosine phosphorylation of TrkA is suppressed by PTP1B WT over-expression, and enhanced by PTP1B Y46F/D181A substrate-trapping mutant. (C) Densitometric quantification of P-TrkA levels normalized to total TrkA. **p
    Inhibitors, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inhibitors/product/Santa Cruz Biotechnology
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    inhibitors - by Bioz Stars, 2020-07
    92/100 stars
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    93
    Santa Cruz Biotechnology na3 vo4
    PTP1B dephosphorylates endocytosed soma surface-derived Trk receptors (A) NGF-induced association of TrkA with PTP1B Y46F/D181A substrate trapping mutant, but not PTP1B WT , in sympathetic neurons. Adenoviruses under Tet-On regulation were used to express PTP1B WT -Myc or PTP1B Y46F/D181A -Myc in sympathetic neurons. No exogenous PTP1B-Myc expression is observed in absence of doxycycline (Dox). <t>Immunoprecipitation</t> was done with anti-Myc, and immunoblotting with anti-Myc or anti-TrkA antibodies. (B) NGF-induced tyrosine phosphorylation of TrkA is suppressed by PTP1B WT over-expression, and enhanced by PTP1B Y46F/D181A substrate-trapping mutant. (C) Densitometric quantification of P-TrkA levels normalized to total TrkA. **p
    Na3 Vo4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Santa Cruz Biotechnology
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology phosphatase
    PTP1B dephosphorylates endocytosed soma surface-derived Trk receptors (A) NGF-induced association of TrkA with PTP1B Y46F/D181A substrate trapping mutant, but not PTP1B WT , in sympathetic neurons. Adenoviruses under Tet-On regulation were used to express PTP1B WT -Myc or PTP1B Y46F/D181A -Myc in sympathetic neurons. No exogenous PTP1B-Myc expression is observed in absence of doxycycline (Dox). <t>Immunoprecipitation</t> was done with anti-Myc, and immunoblotting with anti-Myc or anti-TrkA antibodies. (B) NGF-induced tyrosine phosphorylation of TrkA is suppressed by PTP1B WT over-expression, and enhanced by PTP1B Y46F/D181A substrate-trapping mutant. (C) Densitometric quantification of P-TrkA levels normalized to total TrkA. **p
    Phosphatase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase/product/Santa Cruz Biotechnology
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosphatase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    PTP1B dephosphorylates endocytosed soma surface-derived Trk receptors (A) NGF-induced association of TrkA with PTP1B Y46F/D181A substrate trapping mutant, but not PTP1B WT , in sympathetic neurons. Adenoviruses under Tet-On regulation were used to express PTP1B WT -Myc or PTP1B Y46F/D181A -Myc in sympathetic neurons. No exogenous PTP1B-Myc expression is observed in absence of doxycycline (Dox). Immunoprecipitation was done with anti-Myc, and immunoblotting with anti-Myc or anti-TrkA antibodies. (B) NGF-induced tyrosine phosphorylation of TrkA is suppressed by PTP1B WT over-expression, and enhanced by PTP1B Y46F/D181A substrate-trapping mutant. (C) Densitometric quantification of P-TrkA levels normalized to total TrkA. **p

    Journal: Developmental cell

    Article Title: Phospho-regulation of soma-to-axon transcytosis of neurotrophin receptors

    doi: 10.1016/j.devcel.2017.08.009

    Figure Lengend Snippet: PTP1B dephosphorylates endocytosed soma surface-derived Trk receptors (A) NGF-induced association of TrkA with PTP1B Y46F/D181A substrate trapping mutant, but not PTP1B WT , in sympathetic neurons. Adenoviruses under Tet-On regulation were used to express PTP1B WT -Myc or PTP1B Y46F/D181A -Myc in sympathetic neurons. No exogenous PTP1B-Myc expression is observed in absence of doxycycline (Dox). Immunoprecipitation was done with anti-Myc, and immunoblotting with anti-Myc or anti-TrkA antibodies. (B) NGF-induced tyrosine phosphorylation of TrkA is suppressed by PTP1B WT over-expression, and enhanced by PTP1B Y46F/D181A substrate-trapping mutant. (C) Densitometric quantification of P-TrkA levels normalized to total TrkA. **p

    Article Snippet: After 48 hr, cells were lysed with immunoprecipitation buffer (excluding Na3 VO4 ), and then immunoprecipitated using mouse anti-FLAG antibody (1 μg) and Protein-G agarose beads (Santa Cruz).

    Techniques: Derivative Assay, Mutagenesis, Expressing, Immunoprecipitation, Over Expression