na k atpase β2 subunit  (Millipore)


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    Structured Review

    Millipore na k atpase β2 subunit
    <t>Na,K-ATPase</t> β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained
    Na K Atpase β2 Subunit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase β2 subunit/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    na k atpase β2 subunit - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart"

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.751735

    Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained
    Figure Legend Snippet: Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained

    Techniques Used: Staining

    Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.
    Figure Legend Snippet: Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.

    Techniques Used: Expressing, Purification, Staining, SDS Page

    Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code
    Figure Legend Snippet: Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code

    Techniques Used: Derivative Assay

    The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome
    Figure Legend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome

    Techniques Used: Western Blot, Immunoprecipitation

    The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were
    Figure Legend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were

    Techniques Used:

    Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium
    Figure Legend Snippet: Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium

    Techniques Used: Inhibition, Purification

    Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent
    Figure Legend Snippet: Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent

    Techniques Used: Incubation, Binding Assay

    Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin
    Figure Legend Snippet: Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin

    Techniques Used: Inhibition

    2) Product Images from "Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart"

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.751735

    Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained
    Figure Legend Snippet: Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained

    Techniques Used: Staining

    Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.
    Figure Legend Snippet: Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.

    Techniques Used: Expressing, Purification, Staining, SDS Page

    Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code
    Figure Legend Snippet: Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code

    Techniques Used: Derivative Assay

    The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome
    Figure Legend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome

    Techniques Used: Western Blot, Immunoprecipitation

    The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were
    Figure Legend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were

    Techniques Used:

    Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium
    Figure Legend Snippet: Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium

    Techniques Used: Inhibition, Purification

    Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent
    Figure Legend Snippet: Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent

    Techniques Used: Incubation, Binding Assay

    Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin
    Figure Legend Snippet: Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin

    Techniques Used: Inhibition

    3) Product Images from "Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *"

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.370734

    Localization of the Na,K-ATPase β 2 subunit in nerve sections is similar to that of the Na,K-ATPase α 2 subunit, but not of the Na,K-ATPase α 1 subunit or β 1 subunit. A , frozen sections of rat sciatic nerve were double stained
    Figure Legend Snippet: Localization of the Na,K-ATPase β 2 subunit in nerve sections is similar to that of the Na,K-ATPase α 2 subunit, but not of the Na,K-ATPase α 1 subunit or β 1 subunit. A , frozen sections of rat sciatic nerve were double stained

    Techniques Used: Staining

    The Na,K-ATPase α 2 -β 2 complex is less stable than the Na,K-ATPase α-β 1 or α-β 3 complexes in detergent extracts obtained from mouse brain membranes. A , various concentrations of DDM were used to extract proteins
    Figure Legend Snippet: The Na,K-ATPase α 2 -β 2 complex is less stable than the Na,K-ATPase α-β 1 or α-β 3 complexes in detergent extracts obtained from mouse brain membranes. A , various concentrations of DDM were used to extract proteins

    Techniques Used:

    Localization of the Na,K-ATPase α 1 , α 2 , and β 2 subunits in rat kidney and brain sections. Frozen sections of rat kidney ( A ) and rat brain ( B ) were double-stained by using mouse antibodies against α 1 subunit ( green ) and
    Figure Legend Snippet: Localization of the Na,K-ATPase α 1 , α 2 , and β 2 subunits in rat kidney and brain sections. Frozen sections of rat kidney ( A ) and rat brain ( B ) were double-stained by using mouse antibodies against α 1 subunit ( green ) and

    Techniques Used: Staining

    The Na,K-ATPase α 1 -β 1 complex is more stable than the Na,K-ATPase α 1 -β 2 complex in detergent extracts from MDCK cells. A , MDCK cells stably expressing either YFP-β 1 or YFP-β 2 were lysed by incubation with
    Figure Legend Snippet: The Na,K-ATPase α 1 -β 1 complex is more stable than the Na,K-ATPase α 1 -β 2 complex in detergent extracts from MDCK cells. A , MDCK cells stably expressing either YFP-β 1 or YFP-β 2 were lysed by incubation with

    Techniques Used: Stable Transfection, Expressing, Incubation

    The Na,K-ATPase α 1 -β 2 complex is preserved in digitonin and CHAPS extracts from MDCK cells. A , proteins were extracted from MDCK cells expressing YFP-β 1 , or YFP-β 2 , or YFP-linked bile acid transporter (YFP-NTCP), with the
    Figure Legend Snippet: The Na,K-ATPase α 1 -β 2 complex is preserved in digitonin and CHAPS extracts from MDCK cells. A , proteins were extracted from MDCK cells expressing YFP-β 1 , or YFP-β 2 , or YFP-linked bile acid transporter (YFP-NTCP), with the

    Techniques Used: Expressing

    The Na,K-ATPase α 2 and β 2 subunits are selectively co-immunoprecipitated from mouse brain extracts. Proteins were extracted from mouse brain homogenate by using 1% Nonidet P-40/0.5% DOC. A , the antibodies against the Na,K-ATPase β
    Figure Legend Snippet: The Na,K-ATPase α 2 and β 2 subunits are selectively co-immunoprecipitated from mouse brain extracts. Proteins were extracted from mouse brain homogenate by using 1% Nonidet P-40/0.5% DOC. A , the antibodies against the Na,K-ATPase β

    Techniques Used: Immunoprecipitation

    Related Articles

    Transduction:

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart
    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore). .. For Western blotting analysis, the following monoclonal antibodies were used: against the Na,K-ATPase α1 subunit (mouse, clone C464.6, 1:1000; Millipore), against the Na,K-ATPase β2 subunit (mouse, clone 35; BD Transduction Laboratories), and Na,K-ATPase β3 subunit (goat, 1:500; Santa Cruz Biotechnology, Inc.).

    Surface Biotinylation Assay:

    Article Title: Functional characterization of ABCB4 mutations found in progressive familial intrahepatic cholestasis type 3
    Article Snippet: Paragraph title: Surface biotinylation assay ... A rabbit polyclonal anti-Na+ /K+ ATPase α-1 (EMD Millipore, Billerica, MA, USA) and a goat polyclonal anti-Aldolase A (Santa Cruz Biotechnology) antibodies were used as internal standards.

    Transfection:

    Article Title: Functional characterization of ABCB4 mutations found in progressive familial intrahepatic cholestasis type 3
    Article Snippet: To examine the effect of cyclosporin A on ABCB4 mutants, cells were treated with 10 μM cyclosporin A, 6 h after transfection. .. A rabbit polyclonal anti-Na+ /K+ ATPase α-1 (EMD Millipore, Billerica, MA, USA) and a goat polyclonal anti-Aldolase A (Santa Cruz Biotechnology) antibodies were used as internal standards.

    Article Title: Functional Characterization of ABCB4 Mutations Found in Low Phospholipid-Associated Cholelithiasis (LPAC)
    Article Snippet: Biotinylation of cell surface proteins Biotinylation experiments were conducted using a Cell Surface Protein Isolation Kit (Thermo Fisher Scientific Inc.) according to the manufacturer's protocol using the HEK-293T cells obtaining from transfection of the ABCB4 reference or mutant-bearing plasmids. .. A rabbit polyclonal anti-Na+ /K+ ATPase α-1 antibody (06-520, Millipore) was used as an internal standard.

    Mass Spectrometry:

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex). .. Secondary HRP-conjugated polyclonal antibodies employed were goat anti-mouse (cat. no. 1858413, Pierce Biotechnology) and goat anti-rabbit (cat. no. AP311, The Binding Site).

    Mutagenesis:

    Article Title: Functional characterization of ABCB4 mutations found in progressive familial intrahepatic cholestasis type 3
    Article Snippet: Surface biotinylation assay Biotinylation experiments were conducted using a Cell Surface Protein Isolation Kit (Thermo Fisher Scientific Inc.), performed according to the manufacturer’s protocol, using the cells obtained from transfection of the ABCB4 wild type or mutant-bearing plasmids into HEK-293T cells. .. A rabbit polyclonal anti-Na+ /K+ ATPase α-1 (EMD Millipore, Billerica, MA, USA) and a goat polyclonal anti-Aldolase A (Santa Cruz Biotechnology) antibodies were used as internal standards.

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: The C252S/C253S non-palmitoylated R7BP mutant (R7BP-SS), as described by Drenan and coworkers [ ], was prepared using the QuickChange II Site-Directed Mutagenesis Kit (Stratagene) with the HA-tagged wild-type R7BP in pcDNA3 as the template. .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex).

    Article Title: Functional Characterization of ABCB4 Mutations Found in Low Phospholipid-Associated Cholelithiasis (LPAC)
    Article Snippet: Biotinylation of cell surface proteins Biotinylation experiments were conducted using a Cell Surface Protein Isolation Kit (Thermo Fisher Scientific Inc.) according to the manufacturer's protocol using the HEK-293T cells obtaining from transfection of the ABCB4 reference or mutant-bearing plasmids. .. A rabbit polyclonal anti-Na+ /K+ ATPase α-1 antibody (06-520, Millipore) was used as an internal standard.

    Isolation:

    Article Title: Functional characterization of ABCB4 mutations found in progressive familial intrahepatic cholestasis type 3
    Article Snippet: Surface biotinylation assay Biotinylation experiments were conducted using a Cell Surface Protein Isolation Kit (Thermo Fisher Scientific Inc.), performed according to the manufacturer’s protocol, using the cells obtained from transfection of the ABCB4 wild type or mutant-bearing plasmids into HEK-293T cells. .. A rabbit polyclonal anti-Na+ /K+ ATPase α-1 (EMD Millipore, Billerica, MA, USA) and a goat polyclonal anti-Aldolase A (Santa Cruz Biotechnology) antibodies were used as internal standards.

    Article Title: Functional Characterization of ABCB4 Mutations Found in Low Phospholipid-Associated Cholelithiasis (LPAC)
    Article Snippet: Biotinylation of cell surface proteins Biotinylation experiments were conducted using a Cell Surface Protein Isolation Kit (Thermo Fisher Scientific Inc.) according to the manufacturer's protocol using the HEK-293T cells obtaining from transfection of the ABCB4 reference or mutant-bearing plasmids. .. A rabbit polyclonal anti-Na+ /K+ ATPase α-1 antibody (06-520, Millipore) was used as an internal standard.

    Subcloning:

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: An AU5 epitope-tagged mouse Gβ5 cDNA was prepared by PCR subcloning of residues 2 -353 into the BamHI (5') and EcoRI (3') sites of pcDNA3-AU5 vector thereby adding an in-frame N-terminal AU5 6-residue epitope tag (TDFYLK). .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex).

    Purification:

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex). .. Secondary HRP-conjugated polyclonal antibodies employed were goat anti-mouse (cat. no. 1858413, Pierce Biotechnology) and goat anti-rabbit (cat. no. AP311, The Binding Site).

    Immunoprecipitation:

    Article Title: Nuclear localization of the G protein ?5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein
    Article Snippet: .. Antibodies used for immunoblots and immunoprecipitation were rabbit anti-Gβ5 polyclonal N-terminal antibody ATDG , mouse anti-HA (Covance), mouse anti-α-tubulin (Calibiochem CP06), rabbit anti –Histone H3 (Abcam-Ab 8580), rabbit anti-RGS7 polyclonal antibody 7RC-1 , mouse anti-Na+ /K+ ATPase α1 subunit monoclonal antibody (Abcam, ab7671) and rabbit anti-Na+ /K+ ATPase β2 subunit (Sigma, A3979). .. Secondary antibodies utilized in immunoblots were bovine anti-mouse IgG-HRP-conjugated (Santa Cruz, sc-2380), sheep affinity-purified anti-rabbit IgG (H+L) HRP-conjugated (Binding Site, no. AP311), fluorescein anti-rabbit and anti-mouse IgG (H+L) from Vector Laboratories, nos.

    other:

    Article Title: Ctr1 Is an Apical Copper Transporter in Mammalian Intestinal Epithelial Cells in Vivo That Is Controlled at the Level of Protein Stability *
    Article Snippet: The anti-Na+ /K+ -ATPase α1 subunit antibody was purchased from Millipore (Temecula, CA).

    Expressing:

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: The expression construct encoding full-length bovine RGS7 in pcDNA4-HisMax-C (that adds an in-frame Xpress epitope and His-6 tag) was previously described [ ]. .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex).

    Staining:

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart
    Article Snippet: For immunofluorescent staining, the following monoclonal antibodies were used: Na,K-ATPase α1 subunit (mouse, clone C464.6, 1:20; Millipore) and Na,K-ATPase β1 subunit (mouse, clone M17 P5 F11, 1:100; Affinity Bioreagents). .. The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Polymerase Chain Reaction:

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: An AU5 epitope-tagged mouse Gβ5 cDNA was prepared by PCR subcloning of residues 2 -353 into the BamHI (5') and EcoRI (3') sites of pcDNA3-AU5 vector thereby adding an in-frame N-terminal AU5 6-residue epitope tag (TDFYLK). .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex).

    Western Blot:

    Article Title: Nuclear localization of the G protein ?5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein
    Article Snippet: .. Antibodies used for immunoblots and immunoprecipitation were rabbit anti-Gβ5 polyclonal N-terminal antibody ATDG , mouse anti-HA (Covance), mouse anti-α-tubulin (Calibiochem CP06), rabbit anti –Histone H3 (Abcam-Ab 8580), rabbit anti-RGS7 polyclonal antibody 7RC-1 , mouse anti-Na+ /K+ ATPase α1 subunit monoclonal antibody (Abcam, ab7671) and rabbit anti-Na+ /K+ ATPase β2 subunit (Sigma, A3979). .. Secondary antibodies utilized in immunoblots were bovine anti-mouse IgG-HRP-conjugated (Santa Cruz, sc-2380), sheep affinity-purified anti-rabbit IgG (H+L) HRP-conjugated (Binding Site, no. AP311), fluorescein anti-rabbit and anti-mouse IgG (H+L) from Vector Laboratories, nos.

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart
    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore). .. For Western blotting analysis, the following monoclonal antibodies were used: against the Na,K-ATPase α1 subunit (mouse, clone C464.6, 1:1000; Millipore), against the Na,K-ATPase β2 subunit (mouse, clone 35; BD Transduction Laboratories), and Na,K-ATPase β3 subunit (goat, 1:500; Santa Cruz Biotechnology, Inc.).

    Affinity Purification:

    Article Title: Nuclear localization of the G protein ?5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein
    Article Snippet: Antibodies used for immunoblots and immunoprecipitation were rabbit anti-Gβ5 polyclonal N-terminal antibody ATDG , mouse anti-HA (Covance), mouse anti-α-tubulin (Calibiochem CP06), rabbit anti –Histone H3 (Abcam-Ab 8580), rabbit anti-RGS7 polyclonal antibody 7RC-1 , mouse anti-Na+ /K+ ATPase α1 subunit monoclonal antibody (Abcam, ab7671) and rabbit anti-Na+ /K+ ATPase β2 subunit (Sigma, A3979). .. Secondary antibodies utilized in immunoblots were bovine anti-mouse IgG-HRP-conjugated (Santa Cruz, sc-2380), sheep affinity-purified anti-rabbit IgG (H+L) HRP-conjugated (Binding Site, no. AP311), fluorescein anti-rabbit and anti-mouse IgG (H+L) from Vector Laboratories, nos.

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: .. The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex). .. Secondary HRP-conjugated polyclonal antibodies employed were goat anti-mouse (cat. no. 1858413, Pierce Biotechnology) and goat anti-rabbit (cat. no. AP311, The Binding Site).

    Binding Assay:

    Article Title: Nuclear localization of the G protein ?5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein
    Article Snippet: Antibodies used for immunoblots and immunoprecipitation were rabbit anti-Gβ5 polyclonal N-terminal antibody ATDG , mouse anti-HA (Covance), mouse anti-α-tubulin (Calibiochem CP06), rabbit anti –Histone H3 (Abcam-Ab 8580), rabbit anti-RGS7 polyclonal antibody 7RC-1 , mouse anti-Na+ /K+ ATPase α1 subunit monoclonal antibody (Abcam, ab7671) and rabbit anti-Na+ /K+ ATPase β2 subunit (Sigma, A3979). .. Secondary antibodies utilized in immunoblots were bovine anti-mouse IgG-HRP-conjugated (Santa Cruz, sc-2380), sheep affinity-purified anti-rabbit IgG (H+L) HRP-conjugated (Binding Site, no. AP311), fluorescein anti-rabbit and anti-mouse IgG (H+L) from Vector Laboratories, nos.

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex). .. Secondary HRP-conjugated polyclonal antibodies employed were goat anti-mouse (cat. no. 1858413, Pierce Biotechnology) and goat anti-rabbit (cat. no. AP311, The Binding Site).

    Plasmid Preparation:

    Article Title: Nuclear localization of the G protein ?5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein
    Article Snippet: Antibodies used for immunoblots and immunoprecipitation were rabbit anti-Gβ5 polyclonal N-terminal antibody ATDG , mouse anti-HA (Covance), mouse anti-α-tubulin (Calibiochem CP06), rabbit anti –Histone H3 (Abcam-Ab 8580), rabbit anti-RGS7 polyclonal antibody 7RC-1 , mouse anti-Na+ /K+ ATPase α1 subunit monoclonal antibody (Abcam, ab7671) and rabbit anti-Na+ /K+ ATPase β2 subunit (Sigma, A3979). .. Secondary antibodies utilized in immunoblots were bovine anti-mouse IgG-HRP-conjugated (Santa Cruz, sc-2380), sheep affinity-purified anti-rabbit IgG (H+L) HRP-conjugated (Binding Site, no. AP311), fluorescein anti-rabbit and anti-mouse IgG (H+L) from Vector Laboratories, nos.

    Article Title: R7-binding protein targets the G protein ?5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain
    Article Snippet: Paragraph title: Plasmid construction and antibodies ... The primary antibodies used for immunoblotting were: mouse anti-HA monoclonal antibody (cat. No. MMS-101R, Covance), mouse anti-AU5 monoclonal antibody (cat. No. MMS-135R, Covance), affinity-purified rabbit ATDG polyclonal antibody against the N terminus of Gβ5 [ ], purified IgG fraction of rabbit 7RC-1 polyclonal antibody anti-RGS7 [ ], anti-R7BP N-terminal rabbit antibody (a generous gift from Dr. Kirill Martemyanov), anti-R7BP rabbit polyclonal antibody TRS (raised against the synthetic peptide CTRSSIFQISKPPLQSGDWERRG-amide (corresponding to residues 16 to 37 of human/mouse R7BP, with an added N-terminal cysteine) coupled to maleimide-activated KLH (Pierce), and affinity-purified on a column of synthetic peptide covalently bound to Ultralink Iodoacetyl Gel (Pierce)), mouse anti-flotillin-1 monoclonal antibody (cat. no. 610821, BD Transduction Laboratories), anti-LAT polyclonal rabbit IgG (cat. no. AB4093, Chemicon), anti-PSD-95 affinity-purified polyclonal rabbit antibody (cat. no. AB9634, Chemicon), anti-Na/K ATPase α1 subunit (cat. no. 06–520, Upstate Biotechnology), anti-Na/K ATPase β2 subunit (cat. no. A3979, Sigma), anti-transferrin receptor (TfR) (cat no. 13–6800, Zymed), mouse anti α-tubulin monoclonal antibody (cat. no. CP06, Calbiochem), and mouse anti-p84/N5 5E10 monoclonal (cat. no. MS-P8410-PX1, GeneTex).

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    Millipore anti na k atpase α1 subunit antibody
    Localization of Ctr1 in the jejuna of control and Ctr1 int/int mice in vivo . Jejuna from control mice ( Ctr1 flox/flox or Ctr1 flox/ + ) and Ctr1 int/int P14 mice were subjected to immunohistochemistry ( A–F ) and confocal immunofluorescence microscopy ( G ) analysis. A and B , control, anti-Ctr1 (1:200). C , control, anti-hephaestin (1:100). D and E , Ctr1 int/int , anti-Ctr1 (1:200). F , Ctr1 int/int , anti-hephaestin (1:100). The arrowheads indicate regions of immunopositive signals. G , confocal microscopy of Ctr1 in the jejuna of WT mice; green , anti-Ctr1 (1:200); red , anti-Na + /K + <t>-ATPase</t> <t>α1</t> subunit (1:200); blue , DAPI. A and D , scale bars = 50 μm; B, C, and E–G , scale bars = 20 μm.
    Anti Na K Atpase α1 Subunit Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti na k atpase α1 subunit antibody/product/Millipore
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    Localization of Ctr1 in the jejuna of control and Ctr1 int/int mice in vivo . Jejuna from control mice ( Ctr1 flox/flox or Ctr1 flox/ + ) and Ctr1 int/int P14 mice were subjected to immunohistochemistry ( A–F ) and confocal immunofluorescence microscopy ( G ) analysis. A and B , control, anti-Ctr1 (1:200). C , control, anti-hephaestin (1:100). D and E , Ctr1 int/int , anti-Ctr1 (1:200). F , Ctr1 int/int , anti-hephaestin (1:100). The arrowheads indicate regions of immunopositive signals. G , confocal microscopy of Ctr1 in the jejuna of WT mice; green , anti-Ctr1 (1:200); red , anti-Na + /K + -ATPase α1 subunit (1:200); blue , DAPI. A and D , scale bars = 50 μm; B, C, and E–G , scale bars = 20 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Ctr1 Is an Apical Copper Transporter in Mammalian Intestinal Epithelial Cells in Vivo That Is Controlled at the Level of Protein Stability *

    doi: 10.1074/jbc.M110.143826

    Figure Lengend Snippet: Localization of Ctr1 in the jejuna of control and Ctr1 int/int mice in vivo . Jejuna from control mice ( Ctr1 flox/flox or Ctr1 flox/ + ) and Ctr1 int/int P14 mice were subjected to immunohistochemistry ( A–F ) and confocal immunofluorescence microscopy ( G ) analysis. A and B , control, anti-Ctr1 (1:200). C , control, anti-hephaestin (1:100). D and E , Ctr1 int/int , anti-Ctr1 (1:200). F , Ctr1 int/int , anti-hephaestin (1:100). The arrowheads indicate regions of immunopositive signals. G , confocal microscopy of Ctr1 in the jejuna of WT mice; green , anti-Ctr1 (1:200); red , anti-Na + /K + -ATPase α1 subunit (1:200); blue , DAPI. A and D , scale bars = 50 μm; B, C, and E–G , scale bars = 20 μm.

    Article Snippet: The anti-Na+ /K+ -ATPase α1 subunit antibody was purchased from Millipore (Temecula, CA).

    Techniques: Mouse Assay, In Vivo, Immunohistochemistry, Immunofluorescence, Microscopy, Confocal Microscopy

    Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Staining

    Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Expressing, Purification, Staining, SDS Page

    Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Derivative Assay

    The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Western Blot, Immunoprecipitation

    The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques:

    Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Inhibition, Purification

    Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Incubation, Binding Assay

    Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin

    Article Snippet: The polyclonal antibodies used were Na,K-ATPase α2 subunit (rabbit, 1:200; Millipore) and Na,K-ATPase β2 subunit (rabbit, 1:200; Millipore).

    Techniques: Inhibition

    Localization of the Na,K-ATPase β 2 subunit in nerve sections is similar to that of the Na,K-ATPase α 2 subunit, but not of the Na,K-ATPase α 1 subunit or β 1 subunit. A , frozen sections of rat sciatic nerve were double stained

    Journal: The Journal of Biological Chemistry

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    doi: 10.1074/jbc.M112.370734

    Figure Lengend Snippet: Localization of the Na,K-ATPase β 2 subunit in nerve sections is similar to that of the Na,K-ATPase α 2 subunit, but not of the Na,K-ATPase α 1 subunit or β 1 subunit. A , frozen sections of rat sciatic nerve were double stained

    Article Snippet: For immunofluorescent staining, the monoclonal antibodies against the Na,K-ATPase α1 subunit, clone C464.6 (Millipore) and against the Na,K-ATPase β1 subunit, clone M17-P5-F11 (Affinity Bioreagents) and polyclonal antibodies against the Na,K-ATPase α2 subunit, (Millipore) and against the Na,K-ATPase β2 subunit (Millipore) were used.

    Techniques: Staining

    The Na,K-ATPase α 2 -β 2 complex is less stable than the Na,K-ATPase α-β 1 or α-β 3 complexes in detergent extracts obtained from mouse brain membranes. A , various concentrations of DDM were used to extract proteins

    Journal: The Journal of Biological Chemistry

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    doi: 10.1074/jbc.M112.370734

    Figure Lengend Snippet: The Na,K-ATPase α 2 -β 2 complex is less stable than the Na,K-ATPase α-β 1 or α-β 3 complexes in detergent extracts obtained from mouse brain membranes. A , various concentrations of DDM were used to extract proteins

    Article Snippet: For immunofluorescent staining, the monoclonal antibodies against the Na,K-ATPase α1 subunit, clone C464.6 (Millipore) and against the Na,K-ATPase β1 subunit, clone M17-P5-F11 (Affinity Bioreagents) and polyclonal antibodies against the Na,K-ATPase α2 subunit, (Millipore) and against the Na,K-ATPase β2 subunit (Millipore) were used.

    Techniques:

    Localization of the Na,K-ATPase α 1 , α 2 , and β 2 subunits in rat kidney and brain sections. Frozen sections of rat kidney ( A ) and rat brain ( B ) were double-stained by using mouse antibodies against α 1 subunit ( green ) and

    Journal: The Journal of Biological Chemistry

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    doi: 10.1074/jbc.M112.370734

    Figure Lengend Snippet: Localization of the Na,K-ATPase α 1 , α 2 , and β 2 subunits in rat kidney and brain sections. Frozen sections of rat kidney ( A ) and rat brain ( B ) were double-stained by using mouse antibodies against α 1 subunit ( green ) and

    Article Snippet: For immunofluorescent staining, the monoclonal antibodies against the Na,K-ATPase α1 subunit, clone C464.6 (Millipore) and against the Na,K-ATPase β1 subunit, clone M17-P5-F11 (Affinity Bioreagents) and polyclonal antibodies against the Na,K-ATPase α2 subunit, (Millipore) and against the Na,K-ATPase β2 subunit (Millipore) were used.

    Techniques: Staining

    The Na,K-ATPase α 1 -β 1 complex is more stable than the Na,K-ATPase α 1 -β 2 complex in detergent extracts from MDCK cells. A , MDCK cells stably expressing either YFP-β 1 or YFP-β 2 were lysed by incubation with

    Journal: The Journal of Biological Chemistry

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    doi: 10.1074/jbc.M112.370734

    Figure Lengend Snippet: The Na,K-ATPase α 1 -β 1 complex is more stable than the Na,K-ATPase α 1 -β 2 complex in detergent extracts from MDCK cells. A , MDCK cells stably expressing either YFP-β 1 or YFP-β 2 were lysed by incubation with

    Article Snippet: For immunofluorescent staining, the monoclonal antibodies against the Na,K-ATPase α1 subunit, clone C464.6 (Millipore) and against the Na,K-ATPase β1 subunit, clone M17-P5-F11 (Affinity Bioreagents) and polyclonal antibodies against the Na,K-ATPase α2 subunit, (Millipore) and against the Na,K-ATPase β2 subunit (Millipore) were used.

    Techniques: Stable Transfection, Expressing, Incubation

    The Na,K-ATPase α 1 -β 2 complex is preserved in digitonin and CHAPS extracts from MDCK cells. A , proteins were extracted from MDCK cells expressing YFP-β 1 , or YFP-β 2 , or YFP-linked bile acid transporter (YFP-NTCP), with the

    Journal: The Journal of Biological Chemistry

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    doi: 10.1074/jbc.M112.370734

    Figure Lengend Snippet: The Na,K-ATPase α 1 -β 2 complex is preserved in digitonin and CHAPS extracts from MDCK cells. A , proteins were extracted from MDCK cells expressing YFP-β 1 , or YFP-β 2 , or YFP-linked bile acid transporter (YFP-NTCP), with the

    Article Snippet: For immunofluorescent staining, the monoclonal antibodies against the Na,K-ATPase α1 subunit, clone C464.6 (Millipore) and against the Na,K-ATPase β1 subunit, clone M17-P5-F11 (Affinity Bioreagents) and polyclonal antibodies against the Na,K-ATPase α2 subunit, (Millipore) and against the Na,K-ATPase β2 subunit (Millipore) were used.

    Techniques: Expressing

    The Na,K-ATPase α 2 and β 2 subunits are selectively co-immunoprecipitated from mouse brain extracts. Proteins were extracted from mouse brain homogenate by using 1% Nonidet P-40/0.5% DOC. A , the antibodies against the Na,K-ATPase β

    Journal: The Journal of Biological Chemistry

    Article Title: Subunit Isoform Selectivity in Assembly of Na,K-ATPase ?-? Heterodimers *

    doi: 10.1074/jbc.M112.370734

    Figure Lengend Snippet: The Na,K-ATPase α 2 and β 2 subunits are selectively co-immunoprecipitated from mouse brain extracts. Proteins were extracted from mouse brain homogenate by using 1% Nonidet P-40/0.5% DOC. A , the antibodies against the Na,K-ATPase β

    Article Snippet: For immunofluorescent staining, the monoclonal antibodies against the Na,K-ATPase α1 subunit, clone C464.6 (Millipore) and against the Na,K-ATPase β1 subunit, clone M17-P5-F11 (Affinity Bioreagents) and polyclonal antibodies against the Na,K-ATPase α2 subunit, (Millipore) and against the Na,K-ATPase β2 subunit (Millipore) were used.

    Techniques: Immunoprecipitation