na k atpase α1  (Millipore)


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    Structured Review

    Millipore na k atpase α1
    Expressions of NCX1 ( A ) and the major subunits of Na + /K + <t>-ATPase</t> ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,
    Na K Atpase α1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase α1/product/Millipore
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    na k atpase α1 - by Bioz Stars, 2020-02
    93/100 stars

    Images

    1) Product Images from "Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy"

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00462.2012

    Expressions of NCX1 ( A ) and the major subunits of Na + /K + -ATPase ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,
    Figure Legend Snippet: Expressions of NCX1 ( A ) and the major subunits of Na + /K + -ATPase ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,

    Techniques Used: Isolation

    Schematic presentation of the two parallel cell signaling cascades that are linked to digitalis-inhibited Na + /K + -ATPase of the cardiac myocytes, and their relations to digitalis-induced hypertrophy and positive inotropy. See discussion .
    Figure Legend Snippet: Schematic presentation of the two parallel cell signaling cascades that are linked to digitalis-inhibited Na + /K + -ATPase of the cardiac myocytes, and their relations to digitalis-induced hypertrophy and positive inotropy. See discussion .

    Techniques Used:

    2) Product Images from "A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines"

    Article Title: A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines

    Journal: Oncotarget

    doi: 10.18632/oncotarget.14644

    Cytotoxicity effects of cardiac glycosides and inhibition of Na+/K+-ATPase in four RCC cell lines IC50 values for cytotoxicity and IC50 values for Na+/K+ - ATPase inhibition were related to each other after treatment of the four cell lines with the three cardiac glycosides (A) or only with AMANTADIG (B) .
    Figure Legend Snippet: Cytotoxicity effects of cardiac glycosides and inhibition of Na+/K+-ATPase in four RCC cell lines IC50 values for cytotoxicity and IC50 values for Na+/K+ - ATPase inhibition were related to each other after treatment of the four cell lines with the three cardiac glycosides (A) or only with AMANTADIG (B) .

    Techniques Used: Inhibition

    3) Product Images from "New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification"

    Article Title: New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification

    Journal: ACS Omega

    doi: 10.1021/acsomega.9b03167

    Inhibition of Na + /K + -ATPase activity by DIG, DTPA, DTPA–DIG, and 99m Tc-DTPA–DIG tested at 0.1 μM. Effects of DIG, DTPA–DIG, and 99m Tc-DTPA–DIG on Na + /K + -ATPase activity were assayed with the Na + /K + -ATPase α1,2,3 subunit of the porcine cortex. Results indicated Na + /K + -ATPase inhibition by DIG, DTPA–DIG, and 99m Tc-DTPA–DIG. However, labeling solution, as well as DTPA alone, has no influence on the activity of Na + /K + -ATPase.
    Figure Legend Snippet: Inhibition of Na + /K + -ATPase activity by DIG, DTPA, DTPA–DIG, and 99m Tc-DTPA–DIG tested at 0.1 μM. Effects of DIG, DTPA–DIG, and 99m Tc-DTPA–DIG on Na + /K + -ATPase activity were assayed with the Na + /K + -ATPase α1,2,3 subunit of the porcine cortex. Results indicated Na + /K + -ATPase inhibition by DIG, DTPA–DIG, and 99m Tc-DTPA–DIG. However, labeling solution, as well as DTPA alone, has no influence on the activity of Na + /K + -ATPase.

    Techniques Used: Inhibition, Activity Assay, Labeling

    4) Product Images from "Long-term exposure to hypoxia inhibits tumor progression of lung cancer in rats and mice"

    Article Title: Long-term exposure to hypoxia inhibits tumor progression of lung cancer in rats and mice

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-11-331

    Cell proliferation, apoptosis and Na+-K+ ATPase expression in lung cancer tumors. Immunohistochemistry was used to evaluate cell proliferation and apoptosis. Mitosis was analyzed in slides with H E stain. (A) Percent Ki67 positive cell number for cell proliferation; (B) Mitosis index and (C) Apoptosis. Left panel showing quantitative data and right panel showing representative micrographs. n = 5 for each group. Expression of Na + -K + ATPase α1 in lung cancer tumors and colon cancer tumors (D to F) : Proteins were isolated from the tumors and Western blot was performed for analysis of Na + -K + ATPase α1 expression. Expression of Na + -K + ATPase α1 in lung cancer tumor in rats (D) and in A549 cells and in the lung cancer tumor from hypoxia-pretreated A549 cells in mice (E). (F) Na + -K + ATPase α1 expression in colon cancer tumor in rats (F). n = 3 for each group. Upper panels show representative images and lower panels show quantitative data, setting normoxia as 1. *p
    Figure Legend Snippet: Cell proliferation, apoptosis and Na+-K+ ATPase expression in lung cancer tumors. Immunohistochemistry was used to evaluate cell proliferation and apoptosis. Mitosis was analyzed in slides with H E stain. (A) Percent Ki67 positive cell number for cell proliferation; (B) Mitosis index and (C) Apoptosis. Left panel showing quantitative data and right panel showing representative micrographs. n = 5 for each group. Expression of Na + -K + ATPase α1 in lung cancer tumors and colon cancer tumors (D to F) : Proteins were isolated from the tumors and Western blot was performed for analysis of Na + -K + ATPase α1 expression. Expression of Na + -K + ATPase α1 in lung cancer tumor in rats (D) and in A549 cells and in the lung cancer tumor from hypoxia-pretreated A549 cells in mice (E). (F) Na + -K + ATPase α1 expression in colon cancer tumor in rats (F). n = 3 for each group. Upper panels show representative images and lower panels show quantitative data, setting normoxia as 1. *p

    Techniques Used: Expressing, Immunohistochemistry, Staining, Isolation, Western Blot, Mouse Assay

    Related Articles

    Negative Control:

    Article Title: New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification
    Article Snippet: Na+ /K+ -ATPase Assay Enzymatic activities of the Na+ /K+ -ATPase α1,2,3 subunit of the porcine cortex (Sigma) were assayed using 4 mM ATP as the substrate in a final volume of 40 μL assay buffer containing 40 mM Tris–HCl pH 7.5, 80 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), and 8 mM MgAc2 . .. The negative control was assayed without enzyme, and 4 mM ATP was added after 30 min of incubation at room temperature.

    Article Title: A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines
    Article Snippet: Na+ /K+ -ATPase assay Enzymatic activities of Na+ /K+ -ATPase α1,2,3 subunit of porcine cortex (Sigma) were assayed using 4 mM ATP as substrate in a final volume of 40 μL assay puffer containing 40 mM Tris-HCl pH 7.5, 80 mM NaCl, 1 mM EDTA, 8 mM MgAc2 . .. Negative control was assayed without enzyme and 4 mM ATP was added after 30 min of incubation at room temperature.

    Positive Control:

    Article Title: New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification
    Article Snippet: Na+ /K+ -ATPase Assay Enzymatic activities of the Na+ /K+ -ATPase α1,2,3 subunit of the porcine cortex (Sigma) were assayed using 4 mM ATP as the substrate in a final volume of 40 μL assay buffer containing 40 mM Tris–HCl pH 7.5, 80 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), and 8 mM MgAc2 . .. The positive control contained 0.05 U/mL Na+ /K+ -ATPase α1,2,3 subunits of the porcine cortex in a 30 min preincubated mixture with assay buffer; later, 4 mM ATP was added.

    Article Title: A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines
    Article Snippet: Na+ /K+ -ATPase assay Enzymatic activities of Na+ /K+ -ATPase α1,2,3 subunit of porcine cortex (Sigma) were assayed using 4 mM ATP as substrate in a final volume of 40 μL assay puffer containing 40 mM Tris-HCl pH 7.5, 80 mM NaCl, 1 mM EDTA, 8 mM MgAc2 . .. Positive control contained 0.05 U/mL of Na+ /K+ -ATPase α1, 2, 3 subunit of porcine cortex in a 30 min pre-incubated mixture with assay puffer after 4 mM ATP was added.

    Electrophoresis:

    Article Title: Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells
    Article Snippet: Equal amounts of protein (20 μg) were denatured in sample buffer (Laemmli sample buffer, BIO-RAD #3100010639; Bio-Rad Laboratories, Hercules, CA, USA) and separated by electrophoresis on an 8%–12% gradient gel. .. Proteins on the gel were transferred to a polyvinylidene fluoride membrane which was blocked with 5% dry milk or BSA in TBS (20 mM Tris pH 7.5, 150 mM NaCl) with 0.1% Tween-20 and then incubated with one of the following primary antibodies overnight at 4 °C: Na-K-ATPase α1 (Millipore, # 05-369); Na-K-ATPase β1 (ab2873; Abcam PLC, Cambridge, MA, USA); p-Serine (ab9332; Abcam PLC, Cambridge, MA, USA); p-Threonine (ab179530; Abcam PLC, Cambridge, MA, US); p-Tyrosine (ab9337; Abcam PLC, Cambridge, MA, US); p-Na-K-ATPase α1-Ser23 (Cell Signaling Technology, #4006) and p-Na-K-ATPase α1-Ser16 (Cell Signaling Technology, #4020).

    Activation Assay:

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy
    Article Snippet: Activation of Akt was determined by probing phosphorylated Akt (Ser473) and followed by total Akt. .. Primary antibodies and their sources were as follows: RDI Research Diagnostics, rabbit anti-Na+ /Ca2+ exchanger; BD Transduction Laboratories, anti-PI3K p85; Cell Signaling Technology, rabbit anti-phospho 473-Akt and anti-Akt; Developmental Studies Hybridoma Bank, University of Iowa: Na+ -K+ -ATPase α1 (α6F); ABR, Na+ /K+ -ATPase α2 ; Millipore, Na+ /K+ ATPase β1; Santa Cruz, anti-phospho-ERK and ERK.

    Incubation:

    Article Title: New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification
    Article Snippet: Na+ /K+ -ATPase Assay Enzymatic activities of the Na+ /K+ -ATPase α1,2,3 subunit of the porcine cortex (Sigma) were assayed using 4 mM ATP as the substrate in a final volume of 40 μL assay buffer containing 40 mM Tris–HCl pH 7.5, 80 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), and 8 mM MgAc2 . .. The negative control was assayed without enzyme, and 4 mM ATP was added after 30 min of incubation at room temperature.

    Article Title: A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines
    Article Snippet: Na+ /K+ -ATPase assay Enzymatic activities of Na+ /K+ -ATPase α1,2,3 subunit of porcine cortex (Sigma) were assayed using 4 mM ATP as substrate in a final volume of 40 μL assay puffer containing 40 mM Tris-HCl pH 7.5, 80 mM NaCl, 1 mM EDTA, 8 mM MgAc2 . .. Negative control was assayed without enzyme and 4 mM ATP was added after 30 min of incubation at room temperature.

    Article Title: Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells
    Article Snippet: .. Proteins on the gel were transferred to a polyvinylidene fluoride membrane which was blocked with 5% dry milk or BSA in TBS (20 mM Tris pH 7.5, 150 mM NaCl) with 0.1% Tween-20 and then incubated with one of the following primary antibodies overnight at 4 °C: Na-K-ATPase α1 (Millipore, # 05-369); Na-K-ATPase β1 (ab2873; Abcam PLC, Cambridge, MA, USA); p-Serine (ab9332; Abcam PLC, Cambridge, MA, USA); p-Threonine (ab179530; Abcam PLC, Cambridge, MA, US); p-Tyrosine (ab9337; Abcam PLC, Cambridge, MA, US); p-Na-K-ATPase α1-Ser23 (Cell Signaling Technology, #4006) and p-Na-K-ATPase α1-Ser16 (Cell Signaling Technology, #4020). ..

    Article Title: Long-term exposure to hypoxia inhibits tumor progression of lung cancer in rats and mice
    Article Snippet: Western blot analysis Antibodies included HIF1α and Na+ -K+ ATPase α1 (Sigma) and HIF2α and ß-actin (Santa Cruz Biotechnology). .. Briefly, homogenized tissues were incubated on ice for 30 minutes in lysis buffer and then centrifuged at 14,000 rpm for 10 minutes at 4°C.

    Inhibition:

    Article Title: New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification
    Article Snippet: Na+ /K+ -ATPase Assay Enzymatic activities of the Na+ /K+ -ATPase α1,2,3 subunit of the porcine cortex (Sigma) were assayed using 4 mM ATP as the substrate in a final volume of 40 μL assay buffer containing 40 mM Tris–HCl pH 7.5, 80 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), and 8 mM MgAc2 . .. Inhibition assays were performed by co-incubating the enzyme and individual inhibitor at concentrations of 0.1 μM for 30 min and adding 4 mM ATP solution.

    Article Title: A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines
    Article Snippet: Na+ /K+ -ATPase assay Enzymatic activities of Na+ /K+ -ATPase α1,2,3 subunit of porcine cortex (Sigma) were assayed using 4 mM ATP as substrate in a final volume of 40 μL assay puffer containing 40 mM Tris-HCl pH 7.5, 80 mM NaCl, 1 mM EDTA, 8 mM MgAc2 . .. Inhibition assays were performed by co-incubating enzyme and inhibitor with increasing concentrations from 0.2 μM–1000 μM for 30 min and adding of 4 mM ATP solution.

    Expressing:

    Article Title: Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells
    Article Snippet: Proteins on the gel were transferred to a polyvinylidene fluoride membrane which was blocked with 5% dry milk or BSA in TBS (20 mM Tris pH 7.5, 150 mM NaCl) with 0.1% Tween-20 and then incubated with one of the following primary antibodies overnight at 4 °C: Na-K-ATPase α1 (Millipore, # 05-369); Na-K-ATPase β1 (ab2873; Abcam PLC, Cambridge, MA, USA); p-Serine (ab9332; Abcam PLC, Cambridge, MA, USA); p-Threonine (ab179530; Abcam PLC, Cambridge, MA, US); p-Tyrosine (ab9337; Abcam PLC, Cambridge, MA, US); p-Na-K-ATPase α1-Ser23 (Cell Signaling Technology, #4006) and p-Na-K-ATPase α1-Ser16 (Cell Signaling Technology, #4020). .. The chemiluminescence was detected using a FluorChem M instrument (Alpha Innotech, San Leandro, CA, USA) and its software analyzed the intensity of the bands. β-actin was used to normalize the expression levels in cellular homogenate.

    Western Blot:

    Article Title: Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells
    Article Snippet: Paragraph title: 4.9. Western Blot Analysis ... Proteins on the gel were transferred to a polyvinylidene fluoride membrane which was blocked with 5% dry milk or BSA in TBS (20 mM Tris pH 7.5, 150 mM NaCl) with 0.1% Tween-20 and then incubated with one of the following primary antibodies overnight at 4 °C: Na-K-ATPase α1 (Millipore, # 05-369); Na-K-ATPase β1 (ab2873; Abcam PLC, Cambridge, MA, USA); p-Serine (ab9332; Abcam PLC, Cambridge, MA, USA); p-Threonine (ab179530; Abcam PLC, Cambridge, MA, US); p-Tyrosine (ab9337; Abcam PLC, Cambridge, MA, US); p-Na-K-ATPase α1-Ser23 (Cell Signaling Technology, #4006) and p-Na-K-ATPase α1-Ser16 (Cell Signaling Technology, #4020).

    Article Title: Long-term exposure to hypoxia inhibits tumor progression of lung cancer in rats and mice
    Article Snippet: .. Western blot analysis Antibodies included HIF1α and Na+ -K+ ATPase α1 (Sigma) and HIF2α and ß-actin (Santa Cruz Biotechnology). ..

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy
    Article Snippet: Immunoblots were then quantified and the ratios of p-ERK1/2 to ERK1/2, and p-Akt to Akt band density were calculated. .. Primary antibodies and their sources were as follows: RDI Research Diagnostics, rabbit anti-Na+ /Ca2+ exchanger; BD Transduction Laboratories, anti-PI3K p85; Cell Signaling Technology, rabbit anti-phospho 473-Akt and anti-Akt; Developmental Studies Hybridoma Bank, University of Iowa: Na+ -K+ -ATPase α1 (α6F); ABR, Na+ /K+ -ATPase α2 ; Millipore, Na+ /K+ ATPase β1; Santa Cruz, anti-phospho-ERK and ERK.

    Lysis:

    Article Title: Long-term exposure to hypoxia inhibits tumor progression of lung cancer in rats and mice
    Article Snippet: Western blot analysis Antibodies included HIF1α and Na+ -K+ ATPase α1 (Sigma) and HIF2α and ß-actin (Santa Cruz Biotechnology). .. Briefly, homogenized tissues were incubated on ice for 30 minutes in lysis buffer and then centrifuged at 14,000 rpm for 10 minutes at 4°C.

    ATPase Assay:

    Article Title: New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification
    Article Snippet: .. Na+ /K+ -ATPase Assay Enzymatic activities of the Na+ /K+ -ATPase α1,2,3 subunit of the porcine cortex (Sigma) were assayed using 4 mM ATP as the substrate in a final volume of 40 μL assay buffer containing 40 mM Tris–HCl pH 7.5, 80 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), and 8 mM MgAc2 . .. The negative control was assayed without enzyme, and 4 mM ATP was added after 30 min of incubation at room temperature.

    Article Title: A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)- 1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines
    Article Snippet: .. Na+ /K+ -ATPase assay Enzymatic activities of Na+ /K+ -ATPase α1,2,3 subunit of porcine cortex (Sigma) were assayed using 4 mM ATP as substrate in a final volume of 40 μL assay puffer containing 40 mM Tris-HCl pH 7.5, 80 mM NaCl, 1 mM EDTA, 8 mM MgAc2 . .. Negative control was assayed without enzyme and 4 mM ATP was added after 30 min of incubation at room temperature.

    Software:

    Article Title: Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells
    Article Snippet: Proteins on the gel were transferred to a polyvinylidene fluoride membrane which was blocked with 5% dry milk or BSA in TBS (20 mM Tris pH 7.5, 150 mM NaCl) with 0.1% Tween-20 and then incubated with one of the following primary antibodies overnight at 4 °C: Na-K-ATPase α1 (Millipore, # 05-369); Na-K-ATPase β1 (ab2873; Abcam PLC, Cambridge, MA, USA); p-Serine (ab9332; Abcam PLC, Cambridge, MA, USA); p-Threonine (ab179530; Abcam PLC, Cambridge, MA, US); p-Tyrosine (ab9337; Abcam PLC, Cambridge, MA, US); p-Na-K-ATPase α1-Ser23 (Cell Signaling Technology, #4006) and p-Na-K-ATPase α1-Ser16 (Cell Signaling Technology, #4020). .. The chemiluminescence was detected using a FluorChem M instrument (Alpha Innotech, San Leandro, CA, USA) and its software analyzed the intensity of the bands. β-actin was used to normalize the expression levels in cellular homogenate.

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    Millipore mouse monoclonal anti na k atpase α1 antibody
    Down-regulation of <t>α1</t> <t>Na/K-ATPase</t> in prostate cancer cells. A , α1 expression patterns in paired human normal prostate tissue ( left ) and carcinoma ( right ). Human tissue arrays were immunostained with monoclonal antibody against α1
    Mouse Monoclonal Anti Na K Atpase α1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti na k atpase α1 antibody/product/Millipore
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti na k atpase α1 antibody - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    93
    Millipore na k atpase α1
    Expressions of NCX1 ( A ) and the major subunits of Na + /K + <t>-ATPase</t> ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,
    Na K Atpase α1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase α1/product/Millipore
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    na k atpase α1 - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    83
    Millipore polyclonal rabbit anti α1 na k atpase
    Y260 phosphorylation and Src- (a) Y260 phosphorylation in purified pig kidney <t>α1</t> <t>Na/K-ATPase</t> (2 µg) by Src (4.5 units) in presence of 2 mM Mg 2+ -ATP in 10 minutes. Representative blots are shown, n = 3. Control blots showing Src phosphorylation at Y418 and Y529 sites by Mg 2+ -ATP are shown. (b) Effects of PP2 (5 μM, 30 minutes) on tyrosine phosphorylation of α1 Na/K-ATPase in LLC-PK1 cells. A representative immunoblot is shown. n = 4–5. (c) Effects of Src family kinase knockout on Y260 phosphorylation. Cell lysates were prepared from Src, Yes, Fyn knockout SYF, and Src-rescued SYF cells. Representative blots are shown, n = 4. (d) Effects of ouabain on Y260 phosphorylation as a function of time in LLC-PK1 cells. **p
    Polyclonal Rabbit Anti α1 Na K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti α1 na k atpase/product/Millipore
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti α1 na k atpase - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    Image Search Results


    Down-regulation of α1 Na/K-ATPase in prostate cancer cells. A , α1 expression patterns in paired human normal prostate tissue ( left ) and carcinoma ( right ). Human tissue arrays were immunostained with monoclonal antibody against α1

    Journal: The Journal of Biological Chemistry

    Article Title: Na/K-ATPase Mimetic pNaKtide Peptide Inhibits the Growth of Human Cancer Cells *

    doi: 10.1074/jbc.M110.207597

    Figure Lengend Snippet: Down-regulation of α1 Na/K-ATPase in prostate cancer cells. A , α1 expression patterns in paired human normal prostate tissue ( left ) and carcinoma ( right ). Human tissue arrays were immunostained with monoclonal antibody against α1

    Article Snippet: Tissue sections were incubated with a mouse monoclonal anti-Na/K-ATPase α1 antibody (Millipore) solution (final concentration 3.3 ng/μl) for 1 h at room temperature.

    Techniques: Expressing

    Decreases in surface expression of α1 Na/K-ATPase in prostate cancer cells. A , cellular distribution of α1 Na/K-ATPase. The fixed cells were immunostained with anti-α1 antibody and visualized (in red ) with a Leica DMIRE2 confocal

    Journal: The Journal of Biological Chemistry

    Article Title: Na/K-ATPase Mimetic pNaKtide Peptide Inhibits the Growth of Human Cancer Cells *

    doi: 10.1074/jbc.M110.207597

    Figure Lengend Snippet: Decreases in surface expression of α1 Na/K-ATPase in prostate cancer cells. A , cellular distribution of α1 Na/K-ATPase. The fixed cells were immunostained with anti-α1 antibody and visualized (in red ) with a Leica DMIRE2 confocal

    Article Snippet: Tissue sections were incubated with a mouse monoclonal anti-Na/K-ATPase α1 antibody (Millipore) solution (final concentration 3.3 ng/μl) for 1 h at room temperature.

    Techniques: Expressing

    Rab10 is required for the translocation of Na + /K + -ATPase to the plasma membrane. ( A ) COS-7 cells were transiently transfected with GFP-Rab10-T23N; after 48 hours, cells were serum starved and treated with 100 nM insulin (INS) for 30 minutes. Na + /K + -ATPase

    Journal: Journal of Cell Science

    Article Title: Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt

    doi: 10.1242/jcs.066464

    Figure Lengend Snippet: Rab10 is required for the translocation of Na + /K + -ATPase to the plasma membrane. ( A ) COS-7 cells were transiently transfected with GFP-Rab10-T23N; after 48 hours, cells were serum starved and treated with 100 nM insulin (INS) for 30 minutes. Na + /K + -ATPase

    Article Snippet: The following mouse monoclonal antibodies were purchased: FLAG (Sigma-Aldrich), Na+ /K+ -ATPase α1 subunit (clone 464.6, Millipore), GFP (Santa Cruz Biotechnology), HA (Covance), V5 (Invitrogen) and Rab8 (Transduction Laboratories).

    Techniques: Translocation Assay, Transfection

    Subcellular distribution and colocalization of Rab8, Rab10 or Rab14 with Na + /K + -ATPase in alveolar epithelial cells. ( A ) Total membranes were isolated from GFP-Rab10 A549 cells and the membranes subfractionated on a sucrose gradient as described in Materials

    Journal: Journal of Cell Science

    Article Title: Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt

    doi: 10.1242/jcs.066464

    Figure Lengend Snippet: Subcellular distribution and colocalization of Rab8, Rab10 or Rab14 with Na + /K + -ATPase in alveolar epithelial cells. ( A ) Total membranes were isolated from GFP-Rab10 A549 cells and the membranes subfractionated on a sucrose gradient as described in Materials

    Article Snippet: The following mouse monoclonal antibodies were purchased: FLAG (Sigma-Aldrich), Na+ /K+ -ATPase α1 subunit (clone 464.6, Millipore), GFP (Santa Cruz Biotechnology), HA (Covance), V5 (Invitrogen) and Rab8 (Transduction Laboratories).

    Techniques: Isolation

    Insulin increases Na + /K + -ATPase recruitment to the plasma membrane in a PI3K-dependent way. ( A ) Serum-starved ATII cells were pretreated with genistein (G; 10 μM, 30 minutes preincubation) and then incubated in the absence (CT) or presence of

    Journal: Journal of Cell Science

    Article Title: Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt

    doi: 10.1242/jcs.066464

    Figure Lengend Snippet: Insulin increases Na + /K + -ATPase recruitment to the plasma membrane in a PI3K-dependent way. ( A ) Serum-starved ATII cells were pretreated with genistein (G; 10 μM, 30 minutes preincubation) and then incubated in the absence (CT) or presence of

    Article Snippet: The following mouse monoclonal antibodies were purchased: FLAG (Sigma-Aldrich), Na+ /K+ -ATPase α1 subunit (clone 464.6, Millipore), GFP (Santa Cruz Biotechnology), HA (Covance), V5 (Invitrogen) and Rab8 (Transduction Laboratories).

    Techniques: Incubation

    Insulin increases AFR in isolated perfused rat lungs, and Na + /K + -ATPase activity and recruitment to the plasma membrane in isolated rat ATII cells. ( A ) Rats were injected with insulin (0.1, 0.3 and 1.0 units/kilo) or pretreated with propranolol (P; 10

    Journal: Journal of Cell Science

    Article Title: Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt

    doi: 10.1242/jcs.066464

    Figure Lengend Snippet: Insulin increases AFR in isolated perfused rat lungs, and Na + /K + -ATPase activity and recruitment to the plasma membrane in isolated rat ATII cells. ( A ) Rats were injected with insulin (0.1, 0.3 and 1.0 units/kilo) or pretreated with propranolol (P; 10

    Article Snippet: The following mouse monoclonal antibodies were purchased: FLAG (Sigma-Aldrich), Na+ /K+ -ATPase α1 subunit (clone 464.6, Millipore), GFP (Santa Cruz Biotechnology), HA (Covance), V5 (Invitrogen) and Rab8 (Transduction Laboratories).

    Techniques: Isolation, Activity Assay, Injection

    Insulin-stimulated Na + /K + -ATPase recruitment requires Akt. ( A ) Serum-starved ATII cells were incubated with 100 nM insulin (INS) for 1 or 5 minutes. Then, phosphorylated Akt (p-Akt) or the total amount of Akt was measured by western blot analysis of aliquots

    Journal: Journal of Cell Science

    Article Title: Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt

    doi: 10.1242/jcs.066464

    Figure Lengend Snippet: Insulin-stimulated Na + /K + -ATPase recruitment requires Akt. ( A ) Serum-starved ATII cells were incubated with 100 nM insulin (INS) for 1 or 5 minutes. Then, phosphorylated Akt (p-Akt) or the total amount of Akt was measured by western blot analysis of aliquots

    Article Snippet: The following mouse monoclonal antibodies were purchased: FLAG (Sigma-Aldrich), Na+ /K+ -ATPase α1 subunit (clone 464.6, Millipore), GFP (Santa Cruz Biotechnology), HA (Covance), V5 (Invitrogen) and Rab8 (Transduction Laboratories).

    Techniques: Incubation, Western Blot

    Role of AS160 in insulin-induced Na + /K + -ATPase recruitment to the plasma membrane. ( A ) A549 cells were transiently transfected with FLAG-WT-AS160; 48 hours later, the cells were serum starved and treated with 100 nM insulin for 1 or 5 minutes. Cells were

    Journal: Journal of Cell Science

    Article Title: Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt

    doi: 10.1242/jcs.066464

    Figure Lengend Snippet: Role of AS160 in insulin-induced Na + /K + -ATPase recruitment to the plasma membrane. ( A ) A549 cells were transiently transfected with FLAG-WT-AS160; 48 hours later, the cells were serum starved and treated with 100 nM insulin for 1 or 5 minutes. Cells were

    Article Snippet: The following mouse monoclonal antibodies were purchased: FLAG (Sigma-Aldrich), Na+ /K+ -ATPase α1 subunit (clone 464.6, Millipore), GFP (Santa Cruz Biotechnology), HA (Covance), V5 (Invitrogen) and Rab8 (Transduction Laboratories).

    Techniques: Transfection

    Expressions of NCX1 ( A ) and the major subunits of Na + /K + -ATPase ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy

    doi: 10.1152/ajpheart.00462.2012

    Figure Lengend Snippet: Expressions of NCX1 ( A ) and the major subunits of Na + /K + -ATPase ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,

    Article Snippet: Primary antibodies and their sources were as follows: RDI Research Diagnostics, rabbit anti-Na+ /Ca2+ exchanger; BD Transduction Laboratories, anti-PI3K p85; Cell Signaling Technology, rabbit anti-phospho 473-Akt and anti-Akt; Developmental Studies Hybridoma Bank, University of Iowa: Na+ -K+ -ATPase α1 (α6F); ABR, Na+ /K+ -ATPase α2 ; Millipore, Na+ /K+ ATPase β1; Santa Cruz, anti-phospho-ERK and ERK.

    Techniques: Isolation

    Schematic presentation of the two parallel cell signaling cascades that are linked to digitalis-inhibited Na + /K + -ATPase of the cardiac myocytes, and their relations to digitalis-induced hypertrophy and positive inotropy. See discussion .

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy

    doi: 10.1152/ajpheart.00462.2012

    Figure Lengend Snippet: Schematic presentation of the two parallel cell signaling cascades that are linked to digitalis-inhibited Na + /K + -ATPase of the cardiac myocytes, and their relations to digitalis-induced hypertrophy and positive inotropy. See discussion .

    Article Snippet: Primary antibodies and their sources were as follows: RDI Research Diagnostics, rabbit anti-Na+ /Ca2+ exchanger; BD Transduction Laboratories, anti-PI3K p85; Cell Signaling Technology, rabbit anti-phospho 473-Akt and anti-Akt; Developmental Studies Hybridoma Bank, University of Iowa: Na+ -K+ -ATPase α1 (α6F); ABR, Na+ /K+ -ATPase α2 ; Millipore, Na+ /K+ ATPase β1; Santa Cruz, anti-phospho-ERK and ERK.

    Techniques:

    Y260 phosphorylation and Src- (a) Y260 phosphorylation in purified pig kidney α1 Na/K-ATPase (2 µg) by Src (4.5 units) in presence of 2 mM Mg 2+ -ATP in 10 minutes. Representative blots are shown, n = 3. Control blots showing Src phosphorylation at Y418 and Y529 sites by Mg 2+ -ATP are shown. (b) Effects of PP2 (5 μM, 30 minutes) on tyrosine phosphorylation of α1 Na/K-ATPase in LLC-PK1 cells. A representative immunoblot is shown. n = 4–5. (c) Effects of Src family kinase knockout on Y260 phosphorylation. Cell lysates were prepared from Src, Yes, Fyn knockout SYF, and Src-rescued SYF cells. Representative blots are shown, n = 4. (d) Effects of ouabain on Y260 phosphorylation as a function of time in LLC-PK1 cells. **p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Y260 phosphorylation and Src- (a) Y260 phosphorylation in purified pig kidney α1 Na/K-ATPase (2 µg) by Src (4.5 units) in presence of 2 mM Mg 2+ -ATP in 10 minutes. Representative blots are shown, n = 3. Control blots showing Src phosphorylation at Y418 and Y529 sites by Mg 2+ -ATP are shown. (b) Effects of PP2 (5 μM, 30 minutes) on tyrosine phosphorylation of α1 Na/K-ATPase in LLC-PK1 cells. A representative immunoblot is shown. n = 4–5. (c) Effects of Src family kinase knockout on Y260 phosphorylation. Cell lysates were prepared from Src, Yes, Fyn knockout SYF, and Src-rescued SYF cells. Representative blots are shown, n = 4. (d) Effects of ouabain on Y260 phosphorylation as a function of time in LLC-PK1 cells. **p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Purification, Knock-Out

    Schematic diagrams of α1 Na/K-ATPase-mediated Src regulation in normal and cancer cells. Established signaling pathways are denoted by solid black arrows and speculated signaling pathways are denoted by broken black arrows. NKA = Na/K-ATPase, Cav = Caveolin 1, FAK = Focal Adhesion Kinase, RTK = Receptor Tyrosine Kinase, PI3K = Phosphatidyl Inositol 3 Kinase, ROS = Reactive Oxygen Species, PLC = Phospholipase C, PKC = Protein Kinase C, PKM2 = Pyruvate Kinase isoenzyme M2, PDH = Pyruvate Dehydrogenase, ERK = Extracellular Regulatory Kinase. The circled letter P denotes phosphorylation and activation.

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Schematic diagrams of α1 Na/K-ATPase-mediated Src regulation in normal and cancer cells. Established signaling pathways are denoted by solid black arrows and speculated signaling pathways are denoted by broken black arrows. NKA = Na/K-ATPase, Cav = Caveolin 1, FAK = Focal Adhesion Kinase, RTK = Receptor Tyrosine Kinase, PI3K = Phosphatidyl Inositol 3 Kinase, ROS = Reactive Oxygen Species, PLC = Phospholipase C, PKC = Protein Kinase C, PKM2 = Pyruvate Kinase isoenzyme M2, PDH = Pyruvate Dehydrogenase, ERK = Extracellular Regulatory Kinase. The circled letter P denotes phosphorylation and activation.

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Planar Chromatography, Activation Assay

    Y260 phosphorylation and α1 Na/K-ATPase/Src interaction in human cancers- (a) Measurement of Y260 phosphorylation in cancer cell lines. Cell lysates from a panel of prostate (LNCAP, DU145, and PC3) and breast (MCF7, MDAMB231, and BT-20) cancer cell lines were compared with corresponding control cells (prostate RWPE1 and breast MCF10A), for Na/K-ATPase α1 expression and Y260 phosphorylation. **p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Y260 phosphorylation and α1 Na/K-ATPase/Src interaction in human cancers- (a) Measurement of Y260 phosphorylation in cancer cell lines. Cell lysates from a panel of prostate (LNCAP, DU145, and PC3) and breast (MCF7, MDAMB231, and BT-20) cancer cell lines were compared with corresponding control cells (prostate RWPE1 and breast MCF10A), for Na/K-ATPase α1 expression and Y260 phosphorylation. **p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Expressing

    Identification of Y260 in α1 Na/K-ATPase as the major Src binding site- (a) Comparison of Y260 containing sequences in second cytoplasmic domain (CD2) of different human Na/K-ATPase isoforms. (b) Interaction between CD2 and Src in different cell lines. Representative blots are shown, n = 3. (c) Effects of ouabain on ERK activation. *p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Identification of Y260 in α1 Na/K-ATPase as the major Src binding site- (a) Comparison of Y260 containing sequences in second cytoplasmic domain (CD2) of different human Na/K-ATPase isoforms. (b) Interaction between CD2 and Src in different cell lines. Representative blots are shown, n = 3. (c) Effects of ouabain on ERK activation. *p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Binding Assay, Activation Assay

    Measurement of α1 Na/K-ATPase expression in primary tumor and metastatic lesions- (a) The expression of α1 Na/K-ATPase in prostate cancer. Left panels show α1 expression patterns in paired human normal prostate tissue (left), carcinoma (middle) and bone metastasis (right). Human tissue arrays were immunostained with a α1 monoclonal antibody (in brown). Hematoxylin was used for counterstaining of cell nucleus (in blue). Quantitative data are shown on the right side. **p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Measurement of α1 Na/K-ATPase expression in primary tumor and metastatic lesions- (a) The expression of α1 Na/K-ATPase in prostate cancer. Left panels show α1 expression patterns in paired human normal prostate tissue (left), carcinoma (middle) and bone metastasis (right). Human tissue arrays were immunostained with a α1 monoclonal antibody (in brown). Hematoxylin was used for counterstaining of cell nucleus (in blue). Quantitative data are shown on the right side. **p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Expressing