n4 l arginine hydrochloride  (Millipore)


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    Name:
    Arginine hydrochloride
    Description:
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    Catalog Number:
    a1271000
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    Structured Review

    Millipore n4 l arginine hydrochloride
    Arginine hydrochloride
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    https://www.bioz.com/result/n4 l arginine hydrochloride/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    n4 l arginine hydrochloride - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
    Article Snippet: The speA gene was amplified from the chromosome of the P aeruginosa isolate PA 14 using forward primer 5′-CACC ATGGCCGCTCGACGGACT-3′ and reverse primer 5′- GGACAGGTACGCCGAGCGG-3′ and then cloned into the pBAD Directional TOPO vector as described by the manufacturer (Invitrogen). .. The purified protein was reacted with a 10 mM concentration of 13 C6 ,15 N4 -L-arginine hydrochloride (Sigma), 100 mM HEPES pH 8.4, 5 mM MgS04 , 1 mM DTT, 0.04 mM pyridoxal phosphate at 37°C for 30 min [ ].

    Amplification:

    Article Title: Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
    Article Snippet: The speA gene was amplified from the chromosome of the P aeruginosa isolate PA 14 using forward primer 5′-CACC ATGGCCGCTCGACGGACT-3′ and reverse primer 5′- GGACAGGTACGCCGAGCGG-3′ and then cloned into the pBAD Directional TOPO vector as described by the manufacturer (Invitrogen). .. The purified protein was reacted with a 10 mM concentration of 13 C6 ,15 N4 -L-arginine hydrochloride (Sigma), 100 mM HEPES pH 8.4, 5 mM MgS04 , 1 mM DTT, 0.04 mM pyridoxal phosphate at 37°C for 30 min [ ].

    Expressing:

    Article Title: Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
    Article Snippet: Paragraph title: Expression of speA and synthesis of 13 C5 ,15 N4 -agmatine ... The purified protein was reacted with a 10 mM concentration of 13 C6 ,15 N4 -L-arginine hydrochloride (Sigma), 100 mM HEPES pH 8.4, 5 mM MgS04 , 1 mM DTT, 0.04 mM pyridoxal phosphate at 37°C for 30 min [ ].

    Synthesized:

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: .. Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). ..

    Isolation:

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). .. Chondrocytes were isolated from knee and shoulder joints of mature New Zealand white rabbits and the cells were cultured as previously described [ ].

    Protease Inhibitor:

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: .. Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). ..

    Cell Culture:

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). .. Chondrocytes were isolated from knee and shoulder joints of mature New Zealand white rabbits and the cells were cultured as previously described [ ].

    Mouse Assay:

    Article Title: Correction of ornithine accumulation prevents retinal degeneration in a mouse model of gyrate atrophy of the choroid and retina
    Article Snippet: In brief, mice born from heterozygote matings were given intraperitoneal (i.p.) arginine injections at 12-h intervals starting within hours after birth and continuing for the first 2 weeks of life ( ). .. The arginine stock solution consisted of 1 mol/liter arginine hydrochloride and 1 mol/liter arginine free base (Sigma) at a ratio of 2:1 (vol:vol) with a final concentration of arginine of 1 mol/liter and pH of ≈9.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: .. Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). ..

    Concentration Assay:

    Article Title: Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
    Article Snippet: .. The purified protein was reacted with a 10 mM concentration of 13 C6 ,15 N4 -L-arginine hydrochloride (Sigma), 100 mM HEPES pH 8.4, 5 mM MgS04 , 1 mM DTT, 0.04 mM pyridoxal phosphate at 37°C for 30 min [ ]. .. The reaction was terminated by heat inactivation at 80°C for 10 minutes and the reaction was filtered through a 3 kDa MW cutoff filter from Millipore.

    Article Title: Fluorescent Molecular Rotors as Dyes to Characterize Polysorbate-Containing IgG Formulations
    Article Snippet: The IgG was formulated at a concentration of 1.0 mg/ml in 100 mM sodium phosphate buffer pH 7.2 without or with different concentrations of polysorbate 20 and polysorbate 80 (Fluka, Sigma-Aldrich, Buchs Switzerland). .. Sucrose, mannitol, sodium citrate, sodium citrate dehydrate and citric acid monohydrate were from Fluka (Fluka, Sigma-Aldrich Steinheim, Germany), glycerol from Merck (Merck KGaA, Darmstadt, Germany), arginine hydrochloride from SAFC (SAFC, Sigma-Aldrich Steinheim, Germany), ethanol and methanol from Biosolve (Biosolve B.V., Valkenswaard, The Netherlands), NaH2 PO4 dihydrate, Na2 HPO4 dihydrate and NaCl were from Sigma (Sigma, Sigma-Aldrich Steinheim, Germany).

    Article Title: Correction of ornithine accumulation prevents retinal degeneration in a mouse model of gyrate atrophy of the choroid and retina
    Article Snippet: .. The arginine stock solution consisted of 1 mol/liter arginine hydrochloride and 1 mol/liter arginine free base (Sigma) at a ratio of 2:1 (vol:vol) with a final concentration of arginine of 1 mol/liter and pH of ≈9. .. After 2 weeks of age, the mice no longer require arginine supplementation.

    Article Title: Pharmacological characterization of neurogenic responses of the sheep isolated internal anal sphincter
    Article Snippet: For experiments involving low Ca2+ Krebs-Henseleit solution, a combination of 0.25 m M Ca2+ and 0.5 m M EGTA was used to achieve a Ca2+ free concentration of 0.1 μ M ( ). .. L -arginine hydrochloride, D -arginine hydrochloride, L -NAME ( N G -nitro- L -arginine methyl ester), atropine sulphate, tetrodotoxin (TTX) were obtained from Sigma-Aldrich (U.K.) and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) was obtained from Tocris (Bristol, U.K.).

    Purification:

    Article Title: Antisense knockdown of inducible nitric oxide synthase inhibits the relaxant effect of VIP in isolated smooth muscle cells of the mouse gastric fundus
    Article Snippet: D -arginine hydrochloride, L -arginine hydrochloride, glutamin, glutaraldehyde, Griess reagent, L -NG -nitroarginine ( L -NOARG), penicillin G, sodium fumarate, sodium glutamate, sodium pyruvate, streptomycin, Trypan blue, vasoactive intestinal polypeptide (VIP) and vitamin mixture were from Sigma Chemicals (St. Louis, MO, U.S.A.). .. Recombinant murine tumour necrosis factor alpha (mTNFα) was produced and purified at the Department of Molecular Biology.

    Article Title: Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
    Article Snippet: .. The purified protein was reacted with a 10 mM concentration of 13 C6 ,15 N4 -L-arginine hydrochloride (Sigma), 100 mM HEPES pH 8.4, 5 mM MgS04 , 1 mM DTT, 0.04 mM pyridoxal phosphate at 37°C for 30 min [ ]. .. The reaction was terminated by heat inactivation at 80°C for 10 minutes and the reaction was filtered through a 3 kDa MW cutoff filter from Millipore.

    other:

    Article Title: Common Hepatic Branch of Vagus Nerve-Dependent Expression of Immediate Early Genes in the Mouse Brain by Intraportal L-Arginine: Comparison with Cholecystokinin-8
    Article Snippet: Drugs L-Arginine hydrochloride (Arg), L-N G -nitroarginine methyl ester (L-NAME), L-phenylalanine (Phe), and CCK were purchased from Sigma-Aldrich (St. Louis, MO), and dissolved in saline.

    Article Title: Extracellular L-arginine is required for optimal NO synthesis by eNOS and iNOS in the rat mesenteric artery wall
    Article Snippet: L -arginine hydrochloride, D -arginine hydrochloride, carbamylcholine chloride (carbachol), dexamethasone, gramicidin (from Bacillus brevis ), L -lysine dihydrochloride, phenylephrine hydrochloride and SOD (Cu/Zn-containing enzyme from bovine erythrocytes) were obtained from Sigma (Poole, U.K.).

    Article Title: Small Molecules Attenuate the Interplay between Conformational Fluctuations, Early Oligomerization and Amyloidosis of Alpha Synuclein
    Article Snippet: Arginine hydrochloride and Glutamate sodium salt were obtained from Sigma-Aldrich (St. Louis, MO).

    Article Title: Enhancement of vascular permeability by specific activation of protease-activated receptor-1 in rat hindpaw: a protective role of endogenous and exogenous nitric oxide
    Article Snippet: 647.8), compound 48/80, NG -nitro- L -arginine methyl ester hydrochloride, NG -nitro- D -arginine methyl ester hydrochloride, L -arginine hydrochloride, D -arginine hydrochloride (Sigma, U.S.A.), N-iminoethyl- L -ornithine hydrochloride (Peptide Institute, Japan), sodium nitroprusside (Kishida Chemicals, Japan), and NOC-18 [2,2′-(hydroxynitrosohydrazine) bis -ethanamine; Calbiochem, U.S.A.] were dissolved in physiological saline.

    Article Title: Role of L-arginine in the deficiency of nitric oxide and airway hyperreactivity after the allergen-induced early asthmatic reaction in guinea-pigs
    Article Snippet: Histamine hydrochloride, ovalbumin (grade III), aluminum hydroxide, (−)-isoprenaline hydrochloride, L -arginine hydrochloride, D -arginine hydrochloride and Nω -nitro- L -arginine methyl ester ( L -NAME) were obtained from Sigma Chemical Co. (St. Louis, MO, U.S.A.) and methacholine chloride from Aldrich (Milwaukee, WI, U.S.A.).

    Article Title: Tissue-specific regulation of potassium homeostasis by high doses of cationic amino acids
    Article Snippet: Chemicals l -Arginine hydrochloride, l -lysine hydrochloride, l -ornithine hydrochloride, l -ornithine aspartate, l -glucose and primers were purchased from Sigma Aldrich.

    Recombinant:

    Article Title: Antisense knockdown of inducible nitric oxide synthase inhibits the relaxant effect of VIP in isolated smooth muscle cells of the mouse gastric fundus
    Article Snippet: D -arginine hydrochloride, L -arginine hydrochloride, glutamin, glutaraldehyde, Griess reagent, L -NG -nitroarginine ( L -NOARG), penicillin G, sodium fumarate, sodium glutamate, sodium pyruvate, streptomycin, Trypan blue, vasoactive intestinal polypeptide (VIP) and vitamin mixture were from Sigma Chemicals (St. Louis, MO, U.S.A.). .. Recombinant murine tumour necrosis factor alpha (mTNFα) was produced and purified at the Department of Molecular Biology.

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: .. Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). ..

    Article Title: Leptin increases serotonin turnover by inhibition of brain nitric oxide synthesis
    Article Snippet: Recombinant mouse leptin was a kind gift from Eli Lilly & Co. (Lilly Corporate Center, Indianapolis, Indiana, USA). .. N G -nitro- L -arginine, L -arginine, and D -arginine hydrochloride were obtained from Sigma Chemical Co. (St. Louis, Missouri, USA) and dissolved in CSF for ICV injection and in saline for IP administration.

    Article Title: Fluorescent Molecular Rotors as Dyes to Characterize Polysorbate-Containing IgG Formulations
    Article Snippet: Materials A recombinant humanized monoclonal antibody of the IgG1 subclass (IgG) with a molecular weight of 149 kDa, an isoelectric region between 9 and 10 and an aggregation temperature (Tagg = the onset of aggregation monitored by the optical density at 350 nm during a thermal scan) of about 81°C ( ) was used for the study. .. Sucrose, mannitol, sodium citrate, sodium citrate dehydrate and citric acid monohydrate were from Fluka (Fluka, Sigma-Aldrich Steinheim, Germany), glycerol from Merck (Merck KGaA, Darmstadt, Germany), arginine hydrochloride from SAFC (SAFC, Sigma-Aldrich Steinheim, Germany), ethanol and methanol from Biosolve (Biosolve B.V., Valkenswaard, The Netherlands), NaH2 PO4 dihydrate, Na2 HPO4 dihydrate and NaCl were from Sigma (Sigma, Sigma-Aldrich Steinheim, Germany).

    Plasmid Preparation:

    Article Title: Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
    Article Snippet: The speA gene product was expressed using the arabinose promoter intrinsic to the pBAD vector and purified using the imparted 6-His tag and a nickel embedded agarose slurry per manufacturer instructions (Invitrogen). .. The purified protein was reacted with a 10 mM concentration of 13 C6 ,15 N4 -L-arginine hydrochloride (Sigma), 100 mM HEPES pH 8.4, 5 mM MgS04 , 1 mM DTT, 0.04 mM pyridoxal phosphate at 37°C for 30 min [ ].

    Spectroscopy:

    Article Title: Fluorescent Molecular Rotors as Dyes to Characterize Polysorbate-Containing IgG Formulations
    Article Snippet: Sucrose, mannitol, sodium citrate, sodium citrate dehydrate and citric acid monohydrate were from Fluka (Fluka, Sigma-Aldrich Steinheim, Germany), glycerol from Merck (Merck KGaA, Darmstadt, Germany), arginine hydrochloride from SAFC (SAFC, Sigma-Aldrich Steinheim, Germany), ethanol and methanol from Biosolve (Biosolve B.V., Valkenswaard, The Netherlands), NaH2 PO4 dihydrate, Na2 HPO4 dihydrate and NaCl were from Sigma (Sigma, Sigma-Aldrich Steinheim, Germany). .. The dye content was determined by UV spectroscopy after dilution with deionized water (ANS, Bis-ANS, and CCVJ), ethanol (DCVJ), or DMSO (Nile Red) to an absorbance between 0.1 and 1.0 at the absorption maximum of the respective dye.

    Aqueous Normal-phase Chromatography:

    Article Title: Investigation of the interaction between nitric oxide and vasoactive intestinal polypeptide in the guinea-pig gastric fundus
    Article Snippet: .. Aminoguanidine hemisulphate, atrial natriuretic peptide (ANP), D -arginine hydrochloride, L -arginine hydrochloride, atropine sulphate, bovine serum albumin, dexamethasone, essential amino acid mixture, forskolin, glutamin, glutaraldehyde, guanethidine sulphate, L -NG -nitroarginine ( L -NOARG), (R) -p-cyclic adenosine-3′,5′-monophosphothioate ( (R) -p-cAMPS), sodium nitroprusside (SNP), sodium fumarate, sodium glutamate, sodium pyruvate, trypan blue and vitamin mixture were from Sigma Chemicals (St. Louis, MO, U.S.A.). .. N - 2 - hydroxyethylpiperazine - N′ - 2 - ethanesulphonic acid (HEPES) and soybean trypsin inhibitor were from Boehringer Mannheim Biochemicals (Indianapolis, IN, U.S.A.).

    Modification:

    Article Title: Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition
    Article Snippet: .. Materials and methods Materials were obtained from the following suppliers: New Zealand White rabbits, 5–6 lb (Myrtle's Rabbitry, Thompson Station, TN, USA); modified Eagle's medium (MEM), fetal calf serum (FCS), antibiotics, other tissue culture supplies, and protease inhibitor cocktail for use with mammalian cells (Sigma Chemical, St Louis, MO, USA); DuoSet IC for phospho-p38alpha (R & D Systems, Minneapolis, MN, USA); protein assay reagent (Bio-Rad, Hercules, CA, USA); type 1 collagenase and trypsin (Worthington Biochemical, Freehold, NJ, USA); 35 S-sodium sulfate, 1 Ci/mmol (NEN, Boston, MA, USA); [methyl-3 H]thymidine and prostaglandin E2 (PGE2 ) enzyme immunoassay kits (Amersham Pharmacia Biotech, Piscataway, NJ, USA); human TGF-β1 , human IGF-1, recombinant human IL-1β (R & D Systems, Minneapolis, MN, USA); Sc-58125 (Cayman Chemical, Ann Arbor MI, USA); SB 203580 (SB) and SB 202190, hydrochloride (Calbiochem, San Diego, CA, USA); and N -monomethyl-L -arginine (L -NMA) was synthesized by Dr Paul Dowd and Dr Wei-Zhang (Department of Chemistry, University of Pittsburgh, PA, USA). ..

    Article Title: Pharmacological characterization of neurogenic responses of the sheep isolated internal anal sphincter
    Article Snippet: The composition of the modified Krebs-Henseleit solution was (m M ): NaCl 118, KCl 4.8, MgSO4 .7H2 O 1.2, KH2 PO4 1.2, CaCl2 1.25, NaHCO3 25 and glucose 11.1. .. L -arginine hydrochloride, D -arginine hydrochloride, L -NAME ( N G -nitro- L -arginine methyl ester), atropine sulphate, tetrodotoxin (TTX) were obtained from Sigma-Aldrich (U.K.) and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) was obtained from Tocris (Bristol, U.K.).

    Produced:

    Article Title: Antisense knockdown of inducible nitric oxide synthase inhibits the relaxant effect of VIP in isolated smooth muscle cells of the mouse gastric fundus
    Article Snippet: D -arginine hydrochloride, L -arginine hydrochloride, glutamin, glutaraldehyde, Griess reagent, L -NG -nitroarginine ( L -NOARG), penicillin G, sodium fumarate, sodium glutamate, sodium pyruvate, streptomycin, Trypan blue, vasoactive intestinal polypeptide (VIP) and vitamin mixture were from Sigma Chemicals (St. Louis, MO, U.S.A.). .. Recombinant murine tumour necrosis factor alpha (mTNFα) was produced and purified at the Department of Molecular Biology.

    Injection:

    Article Title: Leptin increases serotonin turnover by inhibition of brain nitric oxide synthesis
    Article Snippet: .. N G -nitro- L -arginine, L -arginine, and D -arginine hydrochloride were obtained from Sigma Chemical Co. (St. Louis, Missouri, USA) and dissolved in CSF for ICV injection and in saline for IP administration. ..

    Binding Assay:

    Article Title: Fluorescent Molecular Rotors as Dyes to Characterize Polysorbate-Containing IgG Formulations
    Article Snippet: The formulations were filtered using 0.22 µm PES low binding syringe driven filter units (MillexTM GP, Millipore, Ireland). .. Sucrose, mannitol, sodium citrate, sodium citrate dehydrate and citric acid monohydrate were from Fluka (Fluka, Sigma-Aldrich Steinheim, Germany), glycerol from Merck (Merck KGaA, Darmstadt, Germany), arginine hydrochloride from SAFC (SAFC, Sigma-Aldrich Steinheim, Germany), ethanol and methanol from Biosolve (Biosolve B.V., Valkenswaard, The Netherlands), NaH2 PO4 dihydrate, Na2 HPO4 dihydrate and NaCl were from Sigma (Sigma, Sigma-Aldrich Steinheim, Germany).

    Molecular Weight:

    Article Title: Fluorescent Molecular Rotors as Dyes to Characterize Polysorbate-Containing IgG Formulations
    Article Snippet: Materials A recombinant humanized monoclonal antibody of the IgG1 subclass (IgG) with a molecular weight of 149 kDa, an isoelectric region between 9 and 10 and an aggregation temperature (Tagg = the onset of aggregation monitored by the optical density at 350 nm during a thermal scan) of about 81°C ( ) was used for the study. .. Sucrose, mannitol, sodium citrate, sodium citrate dehydrate and citric acid monohydrate were from Fluka (Fluka, Sigma-Aldrich Steinheim, Germany), glycerol from Merck (Merck KGaA, Darmstadt, Germany), arginine hydrochloride from SAFC (SAFC, Sigma-Aldrich Steinheim, Germany), ethanol and methanol from Biosolve (Biosolve B.V., Valkenswaard, The Netherlands), NaH2 PO4 dihydrate, Na2 HPO4 dihydrate and NaCl were from Sigma (Sigma, Sigma-Aldrich Steinheim, Germany).

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    Millipore microcystin lr
    Comparison of <t>microcystin-LR</t> (MC-LR) equivalents determined by HPLC with DAD and PP1 inhibition in extracts of laboratory strains and environmental samples of cyanobacteria. Extracts were analyzed by HPLC with DAD, and the results were compared to the results of PP1 analyses of the same extracts performed in the presence of preimmune (null) serum (■) and microcystin-LR antiserum (●), expressed in micrograms per milligram of cyanobacterial cells. The vertical error bars indicate standard deviations where their dimensions exceed those of the symbols. Regression lines are shown for HPLC-DAD analysis versus PP1 inhibition in the presence of preimmune serum (—) ( y = 1.25 x − 0.02; R 2 = 0.94; P
    Microcystin Lr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    microcystin lr - by Bioz Stars, 2020-03
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    Comparison of microcystin-LR (MC-LR) equivalents determined by HPLC with DAD and PP1 inhibition in extracts of laboratory strains and environmental samples of cyanobacteria. Extracts were analyzed by HPLC with DAD, and the results were compared to the results of PP1 analyses of the same extracts performed in the presence of preimmune (null) serum (■) and microcystin-LR antiserum (●), expressed in micrograms per milligram of cyanobacterial cells. The vertical error bars indicate standard deviations where their dimensions exceed those of the symbols. Regression lines are shown for HPLC-DAD analysis versus PP1 inhibition in the presence of preimmune serum (—) ( y = 1.25 x − 0.02; R 2 = 0.94; P

    Journal: Applied and Environmental Microbiology

    Article Title: Colorimetric Immuno-Protein Phosphatase Inhibition Assay for Specific Detection of Microcystins and Nodularins of Cyanobacteria

    doi: 10.1128/AEM.67.2.904-909.2001

    Figure Lengend Snippet: Comparison of microcystin-LR (MC-LR) equivalents determined by HPLC with DAD and PP1 inhibition in extracts of laboratory strains and environmental samples of cyanobacteria. Extracts were analyzed by HPLC with DAD, and the results were compared to the results of PP1 analyses of the same extracts performed in the presence of preimmune (null) serum (■) and microcystin-LR antiserum (●), expressed in micrograms per milligram of cyanobacterial cells. The vertical error bars indicate standard deviations where their dimensions exceed those of the symbols. Regression lines are shown for HPLC-DAD analysis versus PP1 inhibition in the presence of preimmune serum (—) ( y = 1.25 x − 0.02; R 2 = 0.94; P

    Article Snippet: Solutions of purified microcystin-LR (0 to 1,000 μg liter−1 ) were prepared by using Milli-Q (Millipore) water.

    Techniques: High Performance Liquid Chromatography, Inhibition, Environmental Sampling

    ( A ) IgE-specific reactivity (OD 405 nm). Lysates from different M. aeruginosa strains producing varying amounts of microcystin were incubated with pooled sensitized patient sera; BSA was used as a negative control. Abbreviations: –, no microcystin; ++, medium microcystin content (0.1 ng/mL); +++; high microcystin content (241.6 ng/mL); BSA, bovine serum albumin. ( B ) Inhibition of IgE-specific reactivity (OD 405 nm). MC(–) lysates preincubated with extracts containing various concentrations of purified microcystin were incubated with pooled serum from sensitized patients or controls (Ctrl). Data are representative of three separate experiments. * p

    Journal: Environmental Health Perspectives

    Article Title: Identification of Microcystis aeruginosa Peptides Responsible for Allergic Sensitization and Characterization of Functional Interactions between Cyanobacterial Toxins and Immunogenic Peptides

    doi: 10.1289/ehp.1409065

    Figure Lengend Snippet: ( A ) IgE-specific reactivity (OD 405 nm). Lysates from different M. aeruginosa strains producing varying amounts of microcystin were incubated with pooled sensitized patient sera; BSA was used as a negative control. Abbreviations: –, no microcystin; ++, medium microcystin content (0.1 ng/mL); +++; high microcystin content (241.6 ng/mL); BSA, bovine serum albumin. ( B ) Inhibition of IgE-specific reactivity (OD 405 nm). MC(–) lysates preincubated with extracts containing various concentrations of purified microcystin were incubated with pooled serum from sensitized patients or controls (Ctrl). Data are representative of three separate experiments. * p

    Article Snippet: We obtained anti-phycocyanin from Bioss (Woburn, MA), microtiter ELISA plates from Corning (Corning, NY), microcystin LR and anti-FcεRI from EMD Millipore Corporation (Billerica, MA), Eagle’s Minimum Essential Medium (EMEM) from ATCC (Manassas, VA), and phycocyanin, G418, and pNPP substrate from Sigma-Aldrich (St. Louis, MO).

    Techniques: Incubation, Negative Control, Inhibition, Purification

    Effect of PP inhibitors on GC–PC synapse. ( A ) ( Left ) A single GC is electrically stimulated, and eEPSCs are recorded in the postsynaptic PC infused with PP inhibitor. ( Right ) A PC infused with a fluorophore, from a 27-day cerebellar culture. (Scale bar: 15 μm.) ( B1 ) Time course of eEPSC depression induced by 100 nM fostriecin. Synaptic stimulation was started at least 10 min after establishment of the whole-cell configuration to allow for drug diffusion into distal dendrites. ( B2 )( Upper ) eEPSCs from the experiment shown in B1 , at the indicated time points a and b (average of 10 consecutive traces). The vertical transient preceding the eEPSC corresponds to the GC stimulation artifact. ( Lower ) Depressed eEPSC (b) peak-scaled to original amplitude. Note that the eEPSC time course did not change, nor did the passive electrical response to a 3-mV voltage pulse. ( C ) Average decay time course in control condition ( n = 14) and in the presence of fostriecin (100 nM, culture 12–15 DIV, n = 8, and 22–35 DIV, n = 14), cytostatin (10–60 μM, n = 12), microcystin-LR (10 μM, n = 13), thiophosphorylated inhibitor 1 (10–27 units/μl, n = 11), and dephosphocytostatin (30 μM, n = 4).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Protein phosphatase 2A inhibition induces cerebellar long-term depression and declustering of synaptic AMPA receptor

    doi: 10.1073/pnas.0302914101

    Figure Lengend Snippet: Effect of PP inhibitors on GC–PC synapse. ( A ) ( Left ) A single GC is electrically stimulated, and eEPSCs are recorded in the postsynaptic PC infused with PP inhibitor. ( Right ) A PC infused with a fluorophore, from a 27-day cerebellar culture. (Scale bar: 15 μm.) ( B1 ) Time course of eEPSC depression induced by 100 nM fostriecin. Synaptic stimulation was started at least 10 min after establishment of the whole-cell configuration to allow for drug diffusion into distal dendrites. ( B2 )( Upper ) eEPSCs from the experiment shown in B1 , at the indicated time points a and b (average of 10 consecutive traces). The vertical transient preceding the eEPSC corresponds to the GC stimulation artifact. ( Lower ) Depressed eEPSC (b) peak-scaled to original amplitude. Note that the eEPSC time course did not change, nor did the passive electrical response to a 3-mV voltage pulse. ( C ) Average decay time course in control condition ( n = 14) and in the presence of fostriecin (100 nM, culture 12–15 DIV, n = 8, and 22–35 DIV, n = 14), cytostatin (10–60 μM, n = 12), microcystin-LR (10 μM, n = 13), thiophosphorylated inhibitor 1 (10–27 units/μl, n = 11), and dephosphocytostatin (30 μM, n = 4).

    Article Snippet: Microcystin-LR and fostriecin were purchased from Calbiochem, and AMPA, d (-)-2-amino-5-phosphonopentanoic acid, and 7-(hydroxyimino)-cyclopropachromen-1a-carboxylate ethyl ester were from Tocris Cookson (Bristol, U.K.).

    Techniques: Diffusion-based Assay