n2b  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc n2b
    NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, <t>N2B</t> and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p
    N2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n2b/product/Cell Signaling Technology Inc
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    n2b - by Bioz Stars, 2022-10
    94/100 stars

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    1) Product Images from "Low oxygen post conditioning prevents thalamic secondary neuronal loss caused by excitotoxicity after cortical stroke"

    Article Title: Low oxygen post conditioning prevents thalamic secondary neuronal loss caused by excitotoxicity after cortical stroke

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-39493-8

    NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p
    Figure Legend Snippet: NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p

    Techniques Used: Activation Assay, Expressing, Western Blot, Marker, Immunoprecipitation, Incubation

    2) Product Images from "Low oxygen post conditioning prevents thalamic secondary neuronal loss caused by excitotoxicity after cortical stroke"

    Article Title: Low oxygen post conditioning prevents thalamic secondary neuronal loss caused by excitotoxicity after cortical stroke

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-39493-8

    NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p
    Figure Legend Snippet: NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p

    Techniques Used: Activation Assay, Expressing, Western Blot, Marker, Immunoprecipitation, Incubation

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    Cell Signaling Technology Inc n2b
    NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, <t>N2B</t> and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p
    N2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n2b/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n2b - by Bioz Stars, 2022-10
    94/100 stars
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    NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p

    Journal: Scientific Reports

    Article Title: Low oxygen post conditioning prevents thalamic secondary neuronal loss caused by excitotoxicity after cortical stroke

    doi: 10.1038/s41598-019-39493-8

    Figure Lengend Snippet: NMDAR mediated nNOS activation is prevented by LOPC at 15 days. ( A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). ( B ) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. ( C ) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3 . Results are shown as the mean ± SD. *p

    Article Snippet: After transfer and blocking, the membranes were probed with the appropriate antibody: CD11b (Cat#ab75476, Abcam), N2B (Cat#14544, Cell Signalling), NeuN (Cat#MAB377, Millipore), nNOS (Cat#4236, Cell Signalling), NR1 (Cat#5704, Cell Signalling), PSD-95 (Cat#3409, Cell Signalling), Synapsin 1 (Cat#5297, Cell Signalling), β-actin (Cat#A3854, Sigma-Aldrich).

    Techniques: Activation Assay, Expressing, Western Blot, Marker, Immunoprecipitation, Incubation