nω nitro l arginine nna  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore nω nitro l arginine nna
    Effects of Nω <t>-Nitro-L-Arginine</t> <t>(NNA)</t> on IL-1β inducible NF-κB promoter activity, expression and NO production. Caco-2 cells transfected with pNF-κB-Luc vector were treated with IL-1β (4 ng/ml) for 4 hours after pretreatment of NNA (0.5 mM) and L-Arg (5 mM) for 4 hours (Fresh medium with NNA and L-Arg was applied after removing medium for pre-treatment). Cells were harvested for luciferase activity and isolations of protein and total RNA. A: NF-κB luciferase activity is expressed as fold-induction of normalized luciferase activity. B: NF-κB mRNA were measured by qRT-PCR, then normalized to β-actin. C: NF-κB p65 protein was measured by Western blot analysis normalized to β-actin. D: NO levels in cell culture media were measured as described in Methods. NNA alone did not affect the LPS-induced NF-κB promoter activity, NF-κB mRNA and protein expression levels (not shown). Data are means ± SE, * P
    Nω Nitro L Arginine Nna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nω nitro l arginine nna/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nω nitro l arginine nna - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    sodium nitroprusside snp

    Images

    1) Product Images from "L-arginine attenuates Interleukin-1β (IL-1β) induced Nuclear Factor Kappa-Beta (NF-κB) activation in Caco-2 cells"

    Article Title: L-arginine attenuates Interleukin-1β (IL-1β) induced Nuclear Factor Kappa-Beta (NF-κB) activation in Caco-2 cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0174441

    Effects of Nω -Nitro-L-Arginine (NNA) on IL-1β inducible NF-κB promoter activity, expression and NO production. Caco-2 cells transfected with pNF-κB-Luc vector were treated with IL-1β (4 ng/ml) for 4 hours after pretreatment of NNA (0.5 mM) and L-Arg (5 mM) for 4 hours (Fresh medium with NNA and L-Arg was applied after removing medium for pre-treatment). Cells were harvested for luciferase activity and isolations of protein and total RNA. A: NF-κB luciferase activity is expressed as fold-induction of normalized luciferase activity. B: NF-κB mRNA were measured by qRT-PCR, then normalized to β-actin. C: NF-κB p65 protein was measured by Western blot analysis normalized to β-actin. D: NO levels in cell culture media were measured as described in Methods. NNA alone did not affect the LPS-induced NF-κB promoter activity, NF-κB mRNA and protein expression levels (not shown). Data are means ± SE, * P
    Figure Legend Snippet: Effects of Nω -Nitro-L-Arginine (NNA) on IL-1β inducible NF-κB promoter activity, expression and NO production. Caco-2 cells transfected with pNF-κB-Luc vector were treated with IL-1β (4 ng/ml) for 4 hours after pretreatment of NNA (0.5 mM) and L-Arg (5 mM) for 4 hours (Fresh medium with NNA and L-Arg was applied after removing medium for pre-treatment). Cells were harvested for luciferase activity and isolations of protein and total RNA. A: NF-κB luciferase activity is expressed as fold-induction of normalized luciferase activity. B: NF-κB mRNA were measured by qRT-PCR, then normalized to β-actin. C: NF-κB p65 protein was measured by Western blot analysis normalized to β-actin. D: NO levels in cell culture media were measured as described in Methods. NNA alone did not affect the LPS-induced NF-κB promoter activity, NF-κB mRNA and protein expression levels (not shown). Data are means ± SE, * P

    Techniques Used: Activity Assay, Expressing, Transfection, Plasmid Preparation, Luciferase, Quantitative RT-PCR, Western Blot, Cell Culture

    Related Articles

    Autoradiography:

    Article Title: Establishment of Patterned Thalamocortical Connections Does Not Require Nitric Oxide Synthase
    Article Snippet: Nitroarginine (N5501; Sigma) (10 mg/kg per day) and verapamil (verapamil hydrochloride injection, USP; American Regent Laboratories, Shirley, NY) (5 mg/kg per day) were injected daily intraperitoneally into ferrets from P14 to P56 (Table ). .. At P56, ferrets were perfused for [3 H]proline autoradiography.

    Cytometry:

    Article Title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25–35) ☆
    Article Snippet: Measurement of reactive oxygen species by DCFH-DA fluorescence The production of reactive oxygen species was estimated by flow cytometry using the oxidation-sensitive fluorescent probe DCFH-DA[ ], a cell-permeable dye that, once inside the cell, is cleaved by intracellular esterase into its non-fluorescent form DCFH. .. Following exposure to 5 μM Aβ25-35 or Aβ35-25 in the presence or absence of 1 mM diazoxide or 1 mM diazoxide plus 500 μM 5-hydroxydecanoate or 500 μM Nω-nitro-L-arginine or 100 mg/mL catalase (Sigma) or 1 µM H2 O2 (Sigma) for 24 hours, the cultured cells were incubated in serum-free culture medium containing 10 µM DCFH-DA (Molecular Probes Europe) for 30 minutes at 37°C and were then washed with 0.2 M PBS once.

    Blocking Assay:

    Article Title: Activation of cortical 5-HT3 receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions
    Article Snippet: .. To block the nNOS, slices were treated for at least 1 h with an irreversible inhibitor of constitutive nitric oxide synthase (nNOS) and a reversible inhibitor of inducible nitric oxide synthase (iNOS), Nω-Nitro-L-arginine (L-NNA; 100 μM; Sigma). .. NPY Y1 receptors were blocked with the selective antagonist N2-(Diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine amide (BIBP 3226; 100 μM; Sigma).

    Article Title: Establishment of Patterned Thalamocortical Connections Does Not Require Nitric Oxide Synthase
    Article Snippet: Tissue prepared for diaphorase histochemistry was then incubated according to the method of for 1 hr at room temperature in blocking solution containing 1 mg/ml bovine serum albumin (A-7888, Fraction V; Sigma), 0.025% sodium azide (26628-22-8; Mallinckrodt, Paris, KY), 0.3% Triton X-100 (789704; Boehringer Mannheim, Indianapolis, IN), and 1% normal goat serum (Vector Laboratories, Burlingame, CA) in PBS. .. Nitroarginine (N5501; Sigma) (10 mg/kg per day) and verapamil (verapamil hydrochloride injection, USP; American Regent Laboratories, Shirley, NY) (5 mg/kg per day) were injected daily intraperitoneally into ferrets from P14 to P56 (Table ).

    Incubation:

    Article Title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25–35) ☆
    Article Snippet: .. Following exposure to 5 μM Aβ25-35 or Aβ35-25 in the presence or absence of 1 mM diazoxide or 1 mM diazoxide plus 500 μM 5-hydroxydecanoate or 500 μM Nω-nitro-L-arginine or 100 mg/mL catalase (Sigma) or 1 µM H2 O2 (Sigma) for 24 hours, the cultured cells were incubated in serum-free culture medium containing 10 µM DCFH-DA (Molecular Probes Europe) for 30 minutes at 37°C and were then washed with 0.2 M PBS once. .. Cells were then trypsinized and washed, followed by flow cytometer detection using a FACS Calibur cytometry instrument (BD Biosciences, San Jose, CA, USA).

    Article Title: Prion Protein and Copper Cooperatively Protect Neurons by Modulating NMDA Receptor Through S-nitrosylation
    Article Snippet: The drug stock solutions were prepared as follows: 1 mM NMDA (0114; Tocris Biosciences, Missouri, United Kingdom) in ddH2 O stored at −20°C; 250 mM EGTA (E4378; Sigma-Aldrich) in phosphate-buffered saline (PBS), pH 7.4, stored at RT; 40 mM CNQX (C239; Sigma-Aldrich) in dimethyl sulfoxide (DMSO, D8418; Sigma-Aldrich) stored at −20°C; 10 mM (2R )-amino-5-phosphonovaleric acid (AP5, A5282; Sigma-Aldrich) in ddH2 O stored at −20°C; 5 mM threo ifenprodil hemitartrate (2892; Tocris Biosciences) in ddH2 O stored at −20°C; 10 mM GSNO (N4148; Sigma-Aldrich) in ddH2 O stored at −20°C; freshly prepared three NNA (N5501; Sigma-Aldrich) in serum-free medium; freshly prepared 4 mM CZ in 50% ethanol. .. Treatments with 5 μM NMDA in combination with either 50 μM AP5 or 20 μM CNQX or 20 μM GSNO or 1 mM NNA or 20 μM CZ were incubated for 3 h. To obtain a more complete inhibition, A P5, CNQX, and NNA were preincubated for 30 min before adding NMDA.

    Article Title: Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes
    Article Snippet: NO assay For in vitro assays, M Lung and M Bone Marrow cell lines, or BAE were incubated for 6 h, 16 h and 24 h with T. congolense IL3000 strain and washed in PBS. .. This assay was also performed after addition of 1 mM Nω-Nitro-L-arginine (Sigma), a competitive inhibitor of NO synthase.

    Article Title: Establishment of Patterned Thalamocortical Connections Does Not Require Nitric Oxide Synthase
    Article Snippet: Longer incubation times tended to stain cells intensely, preventing discrimination of blue from brown reaction products. .. Nitroarginine (N5501; Sigma) (10 mg/kg per day) and verapamil (verapamil hydrochloride injection, USP; American Regent Laboratories, Shirley, NY) (5 mg/kg per day) were injected daily intraperitoneally into ferrets from P14 to P56 (Table ).

    Activity Assay:

    Article Title: Comparison of mechanisms involved in impaired vascular reactivity between high sucrose and high fat diets in rats
    Article Snippet: This compound is an SOD mimetic with enhanced catalase activity [ ]. .. Inhibition of nitric oxide synthase, using 100 μM Nω-nitro-L-arginine (LNNA; Sigma), was performed to determine the role of NO in ACh-induced vasodilation.

    Expressing:

    Article Title: Activation of cortical 5-HT3 receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions
    Article Snippet: Drugs The 5-HT3 receptor selective agonist (1-(3-Chlorophenyl)biguanide hydrochloride, mCPBG, 100 μM, Sigma) was used to stimulate specifically 5-HT3 -expressing interneurons. .. To block the nNOS, slices were treated for at least 1 h with an irreversible inhibitor of constitutive nitric oxide synthase (nNOS) and a reversible inhibitor of inducible nitric oxide synthase (iNOS), Nω-Nitro-L-arginine (L-NNA; 100 μM; Sigma).

    Flow Cytometry:

    Article Title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25–35) ☆
    Article Snippet: Measurement of reactive oxygen species by DCFH-DA fluorescence The production of reactive oxygen species was estimated by flow cytometry using the oxidation-sensitive fluorescent probe DCFH-DA[ ], a cell-permeable dye that, once inside the cell, is cleaved by intracellular esterase into its non-fluorescent form DCFH. .. Following exposure to 5 μM Aβ25-35 or Aβ35-25 in the presence or absence of 1 mM diazoxide or 1 mM diazoxide plus 500 μM 5-hydroxydecanoate or 500 μM Nω-nitro-L-arginine or 100 mg/mL catalase (Sigma) or 1 µM H2 O2 (Sigma) for 24 hours, the cultured cells were incubated in serum-free culture medium containing 10 µM DCFH-DA (Molecular Probes Europe) for 30 minutes at 37°C and were then washed with 0.2 M PBS once.

    Dissection:

    Article Title: Prion Protein and Copper Cooperatively Protect Neurons by Modulating NMDA Receptor Through S-nitrosylation
    Article Snippet: After washing in the dissection medium for 40 min at 4°C, slices showing an intact hippocampal cytoarchitecture were selected, singularly attached to a coverslip by embedding in a chicken plasma (P3266; Sigma-Aldrich) and thrombin clot (112374; Merck KGaA, Darmstadt, Germany) and maintained at 10 rotations per hour at 37°C in Nunc cell culture tubes (156758; Thermoscientific) with a medium composed of 50% basal medium Eagle (41010026; Gibco, Carlsbad, CA), 25% horse serum (26050-088; Gibco), 25% Hank's balanced salt solution (24020141; Gibco), 5.6 mM d -glucose, 2 mM l -glutamine (25030-032; Gibco). .. The drug stock solutions were prepared as follows: 1 mM NMDA (0114; Tocris Biosciences, Missouri, United Kingdom) in ddH2 O stored at −20°C; 250 mM EGTA (E4378; Sigma-Aldrich) in phosphate-buffered saline (PBS), pH 7.4, stored at RT; 40 mM CNQX (C239; Sigma-Aldrich) in dimethyl sulfoxide (DMSO, D8418; Sigma-Aldrich) stored at −20°C; 10 mM (2R )-amino-5-phosphonovaleric acid (AP5, A5282; Sigma-Aldrich) in ddH2 O stored at −20°C; 5 mM threo ifenprodil hemitartrate (2892; Tocris Biosciences) in ddH2 O stored at −20°C; 10 mM GSNO (N4148; Sigma-Aldrich) in ddH2 O stored at −20°C; freshly prepared three NNA (N5501; Sigma-Aldrich) in serum-free medium; freshly prepared 4 mM CZ in 50% ethanol.

    Cell Culture:

    Article Title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25–35) ☆
    Article Snippet: .. Following exposure to 5 μM Aβ25-35 or Aβ35-25 in the presence or absence of 1 mM diazoxide or 1 mM diazoxide plus 500 μM 5-hydroxydecanoate or 500 μM Nω-nitro-L-arginine or 100 mg/mL catalase (Sigma) or 1 µM H2 O2 (Sigma) for 24 hours, the cultured cells were incubated in serum-free culture medium containing 10 µM DCFH-DA (Molecular Probes Europe) for 30 minutes at 37°C and were then washed with 0.2 M PBS once. .. Cells were then trypsinized and washed, followed by flow cytometer detection using a FACS Calibur cytometry instrument (BD Biosciences, San Jose, CA, USA).

    Article Title: Prion Protein and Copper Cooperatively Protect Neurons by Modulating NMDA Receptor Through S-nitrosylation
    Article Snippet: After washing in the dissection medium for 40 min at 4°C, slices showing an intact hippocampal cytoarchitecture were selected, singularly attached to a coverslip by embedding in a chicken plasma (P3266; Sigma-Aldrich) and thrombin clot (112374; Merck KGaA, Darmstadt, Germany) and maintained at 10 rotations per hour at 37°C in Nunc cell culture tubes (156758; Thermoscientific) with a medium composed of 50% basal medium Eagle (41010026; Gibco, Carlsbad, CA), 25% horse serum (26050-088; Gibco), 25% Hank's balanced salt solution (24020141; Gibco), 5.6 mM d -glucose, 2 mM l -glutamine (25030-032; Gibco). .. The drug stock solutions were prepared as follows: 1 mM NMDA (0114; Tocris Biosciences, Missouri, United Kingdom) in ddH2 O stored at −20°C; 250 mM EGTA (E4378; Sigma-Aldrich) in phosphate-buffered saline (PBS), pH 7.4, stored at RT; 40 mM CNQX (C239; Sigma-Aldrich) in dimethyl sulfoxide (DMSO, D8418; Sigma-Aldrich) stored at −20°C; 10 mM (2R )-amino-5-phosphonovaleric acid (AP5, A5282; Sigma-Aldrich) in ddH2 O stored at −20°C; 5 mM threo ifenprodil hemitartrate (2892; Tocris Biosciences) in ddH2 O stored at −20°C; 10 mM GSNO (N4148; Sigma-Aldrich) in ddH2 O stored at −20°C; freshly prepared three NNA (N5501; Sigma-Aldrich) in serum-free medium; freshly prepared 4 mM CZ in 50% ethanol.

    Inhibition:

    Article Title: Prion Protein and Copper Cooperatively Protect Neurons by Modulating NMDA Receptor Through S-nitrosylation
    Article Snippet: The drug stock solutions were prepared as follows: 1 mM NMDA (0114; Tocris Biosciences, Missouri, United Kingdom) in ddH2 O stored at −20°C; 250 mM EGTA (E4378; Sigma-Aldrich) in phosphate-buffered saline (PBS), pH 7.4, stored at RT; 40 mM CNQX (C239; Sigma-Aldrich) in dimethyl sulfoxide (DMSO, D8418; Sigma-Aldrich) stored at −20°C; 10 mM (2R )-amino-5-phosphonovaleric acid (AP5, A5282; Sigma-Aldrich) in ddH2 O stored at −20°C; 5 mM threo ifenprodil hemitartrate (2892; Tocris Biosciences) in ddH2 O stored at −20°C; 10 mM GSNO (N4148; Sigma-Aldrich) in ddH2 O stored at −20°C; freshly prepared three NNA (N5501; Sigma-Aldrich) in serum-free medium; freshly prepared 4 mM CZ in 50% ethanol. .. Treatments with 5 μM NMDA in combination with either 50 μM AP5 or 20 μM CNQX or 20 μM GSNO or 1 mM NNA or 20 μM CZ were incubated for 3 h. To obtain a more complete inhibition, A P5, CNQX, and NNA were preincubated for 30 min before adding NMDA.

    Article Title: Comparison of mechanisms involved in impaired vascular reactivity between high sucrose and high fat diets in rats
    Article Snippet: .. Inhibition of nitric oxide synthase, using 100 μM Nω-nitro-L-arginine (LNNA; Sigma), was performed to determine the role of NO in ACh-induced vasodilation. ..

    Transmission Assay:

    Article Title: Activation of cortical 5-HT3 receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions
    Article Snippet: To block neuronal synaptic transmission, mCPBG was applied in the presence and after 10 min application of tetrodotoxin (TTX; 1 μM; Latoxan). .. To block the nNOS, slices were treated for at least 1 h with an irreversible inhibitor of constitutive nitric oxide synthase (nNOS) and a reversible inhibitor of inducible nitric oxide synthase (iNOS), Nω-Nitro-L-arginine (L-NNA; 100 μM; Sigma).

    Injection:

    Article Title: Establishment of Patterned Thalamocortical Connections Does Not Require Nitric Oxide Synthase
    Article Snippet: .. Nitroarginine (N5501; Sigma) (10 mg/kg per day) and verapamil (verapamil hydrochloride injection, USP; American Regent Laboratories, Shirley, NY) (5 mg/kg per day) were injected daily intraperitoneally into ferrets from P14 to P56 (Table ). .. [In earlier experiments, ferrets were injected from P20 to P56 with nitroarginine alone (Table ).]

    Recombinant:

    Article Title: Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants
    Article Snippet: .. The conditions for the three assays were as follows: 1 ) room air with and without recombinant human VEGF (rhVEGF; 25 ng/ml; R & D Systems 293-VE); hyperoxia with and without rhVEGF; 2 ) room air with and without NO gas (10 ppm) and hyperoxia with and without NO gas; and 3 ) room air and hyperoxia with the combination of rhVEGF with or without N ω -nitro- l -arginine (LNA; 2 mmol/l; Sigma N5501) to inhibit NO production. .. Concentrations of rhVEGF, NO gas, and LNA were established by preliminary in vitro studies and earlier publications ( , , , ).

    In Vivo:

    Article Title: Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes
    Article Snippet: This assay was also performed after addition of 1 mM Nω-Nitro-L-arginine (Sigma), a competitive inhibitor of NO synthase. .. For in vivo assays, total nitrate and nitrite levels in mice sera were measured using a fluorometric kit (Calbiochem) according to the manufacturer's instructions.

    Fluorescence:

    Article Title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25–35) ☆
    Article Snippet: Paragraph title: Measurement of reactive oxygen species by DCFH-DA fluorescence ... Following exposure to 5 μM Aβ25-35 or Aβ35-25 in the presence or absence of 1 mM diazoxide or 1 mM diazoxide plus 500 μM 5-hydroxydecanoate or 500 μM Nω-nitro-L-arginine or 100 mg/mL catalase (Sigma) or 1 µM H2 O2 (Sigma) for 24 hours, the cultured cells were incubated in serum-free culture medium containing 10 µM DCFH-DA (Molecular Probes Europe) for 30 minutes at 37°C and were then washed with 0.2 M PBS once.

    Article Title: Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes
    Article Snippet: Medium was removed, cells were washed in PBS for 15 min, and fluorescence intensity was measured with an Optima plate reader (BMG Labtech, Germany) (Excitation/Emission: 485/520). .. This assay was also performed after addition of 1 mM Nω-Nitro-L-arginine (Sigma), a competitive inhibitor of NO synthase.

    Growth Assay:

    Article Title: Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants
    Article Snippet: Paragraph title: ECFC growth assay. ... The conditions for the three assays were as follows: 1 ) room air with and without recombinant human VEGF (rhVEGF; 25 ng/ml; R & D Systems 293-VE); hyperoxia with and without rhVEGF; 2 ) room air with and without NO gas (10 ppm) and hyperoxia with and without NO gas; and 3 ) room air and hyperoxia with the combination of rhVEGF with or without N ω -nitro- l -arginine (LNA; 2 mmol/l; Sigma N5501) to inhibit NO production.

    Immunohistochemistry:

    Article Title: Establishment of Patterned Thalamocortical Connections Does Not Require Nitric Oxide Synthase
    Article Snippet: For sections used for both diaphorase and NOS immunohistochemistry, diaphorase was performed first, with incubation times shortened to facilitate visualization of both blue and brown products within the same cells. .. Nitroarginine (N5501; Sigma) (10 mg/kg per day) and verapamil (verapamil hydrochloride injection, USP; American Regent Laboratories, Shirley, NY) (5 mg/kg per day) were injected daily intraperitoneally into ferrets from P14 to P56 (Table ).

    Mouse Assay:

    Article Title: Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes
    Article Snippet: This assay was also performed after addition of 1 mM Nω-Nitro-L-arginine (Sigma), a competitive inhibitor of NO synthase. .. For in vivo assays, total nitrate and nitrite levels in mice sera were measured using a fluorometric kit (Calbiochem) according to the manufacturer's instructions.

    Staining:

    Article Title: Establishment of Patterned Thalamocortical Connections Does Not Require Nitric Oxide Synthase
    Article Snippet: Longer incubation times tended to stain cells intensely, preventing discrimination of blue from brown reaction products. .. Nitroarginine (N5501; Sigma) (10 mg/kg per day) and verapamil (verapamil hydrochloride injection, USP; American Regent Laboratories, Shirley, NY) (5 mg/kg per day) were injected daily intraperitoneally into ferrets from P14 to P56 (Table ).

    Software:

    Article Title: Comparison of mechanisms involved in impaired vascular reactivity between high sucrose and high fat diets in rats
    Article Snippet: Data were digitized using DATAQ A/D software for analysis (DATAQ Instruments, Akron, OH). .. Inhibition of nitric oxide synthase, using 100 μM Nω-nitro-L-arginine (LNNA; Sigma), was performed to determine the role of NO in ACh-induced vasodilation.

    In Vitro:

    Article Title: Endothelin-1 decreases endothelial PPARγ signaling and impairs angiogenesis after chronic intrauterine pulmonary hypertension
    Article Snippet: The ability of fetal PAECs to form vascular structures in vitro was assayed by plating of PAECs on type 1 bovine collagen (5 mg/ml; R & D Systems, Minneapolis, MN; catalog no. 3442-050-01) for 5–7 days. .. A collagen-cell suspension consisting of collagen (1.25 mg/ml) and PAECs from normal and PPHN fetal sheep (2.5 × 105 cells/well) was pipetted into 24-well tissue culture dishes (500 μl/well) and allowed to polymerize at 37°C for 1 h. After polymerization, DMEM with 10% FBS, supplemented with rosiglitazone (Rosi; 100 μM; Cayman Chemical, Ann Arbor, MI catalog no. 71740), 15deoxy-Δ12,14 prostaglandin J2 (PG-J2; 5 μM; Cayman Chemical, catalog no. 18570), with and without N -nitro- l -arginine (LNA) (an NOS inhibitor; 4 mM; Sigma, St. Louis, MO; catalog no. N5501) and ET-1 (100 nM; Sigma, catalog no. E7764) was added to each well depending on study conditions.

    Article Title: Prion Protein and Copper Cooperatively Protect Neurons by Modulating NMDA Receptor Through S-nitrosylation
    Article Snippet: After 13 days in vitro , OHC were treated in the serum-free medium, as described in . .. The drug stock solutions were prepared as follows: 1 mM NMDA (0114; Tocris Biosciences, Missouri, United Kingdom) in ddH2 O stored at −20°C; 250 mM EGTA (E4378; Sigma-Aldrich) in phosphate-buffered saline (PBS), pH 7.4, stored at RT; 40 mM CNQX (C239; Sigma-Aldrich) in dimethyl sulfoxide (DMSO, D8418; Sigma-Aldrich) stored at −20°C; 10 mM (2R )-amino-5-phosphonovaleric acid (AP5, A5282; Sigma-Aldrich) in ddH2 O stored at −20°C; 5 mM threo ifenprodil hemitartrate (2892; Tocris Biosciences) in ddH2 O stored at −20°C; 10 mM GSNO (N4148; Sigma-Aldrich) in ddH2 O stored at −20°C; freshly prepared three NNA (N5501; Sigma-Aldrich) in serum-free medium; freshly prepared 4 mM CZ in 50% ethanol.

    Article Title: Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants
    Article Snippet: The conditions for the three assays were as follows: 1 ) room air with and without recombinant human VEGF (rhVEGF; 25 ng/ml; R & D Systems 293-VE); hyperoxia with and without rhVEGF; 2 ) room air with and without NO gas (10 ppm) and hyperoxia with and without NO gas; and 3 ) room air and hyperoxia with the combination of rhVEGF with or without N ω -nitro- l -arginine (LNA; 2 mmol/l; Sigma N5501) to inhibit NO production. .. Concentrations of rhVEGF, NO gas, and LNA were established by preliminary in vitro studies and earlier publications ( , , , ).

    Article Title: Identification of Trans-Sialidases as a Common Mediator of Endothelial Cell Activation by African Trypanosomes
    Article Snippet: NO assay For in vitro assays, M Lung and M Bone Marrow cell lines, or BAE were incubated for 6 h, 16 h and 24 h with T. congolense IL3000 strain and washed in PBS. .. This assay was also performed after addition of 1 mM Nω-Nitro-L-arginine (Sigma), a competitive inhibitor of NO synthase.

    Tube Formation Assay:

    Article Title: Endothelin-1 decreases endothelial PPARγ signaling and impairs angiogenesis after chronic intrauterine pulmonary hypertension
    Article Snippet: Paragraph title: Tube formation assay. ... A collagen-cell suspension consisting of collagen (1.25 mg/ml) and PAECs from normal and PPHN fetal sheep (2.5 × 105 cells/well) was pipetted into 24-well tissue culture dishes (500 μl/well) and allowed to polymerize at 37°C for 1 h. After polymerization, DMEM with 10% FBS, supplemented with rosiglitazone (Rosi; 100 μM; Cayman Chemical, Ann Arbor, MI catalog no. 71740), 15deoxy-Δ12,14 prostaglandin J2 (PG-J2; 5 μM; Cayman Chemical, catalog no. 18570), with and without N -nitro- l -arginine (LNA) (an NOS inhibitor; 4 mM; Sigma, St. Louis, MO; catalog no. N5501) and ET-1 (100 nM; Sigma, catalog no. E7764) was added to each well depending on study conditions.

    FACS:

    Article Title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25–35) ☆
    Article Snippet: Following exposure to 5 μM Aβ25-35 or Aβ35-25 in the presence or absence of 1 mM diazoxide or 1 mM diazoxide plus 500 μM 5-hydroxydecanoate or 500 μM Nω-nitro-L-arginine or 100 mg/mL catalase (Sigma) or 1 µM H2 O2 (Sigma) for 24 hours, the cultured cells were incubated in serum-free culture medium containing 10 µM DCFH-DA (Molecular Probes Europe) for 30 minutes at 37°C and were then washed with 0.2 M PBS once. .. Cells were then trypsinized and washed, followed by flow cytometer detection using a FACS Calibur cytometry instrument (BD Biosciences, San Jose, CA, USA).

    other:

    Article Title: Ex vivo biomechanical, functional, and immunohistochemical alterations of adrenergic responses in the female urethra in a rat model of birth trauma
    Article Snippet: The agents used to test the tissue in the active state were added consecutively to the external bath ( ): 100 μM N ω -nitro- l -arginine (NOSi; Ref. ), a nitric oxide synthase inhibitor (no. N5501; Sigma Chemical), and 40 μM PE , a nonselective α1 -adrenergic receptor agonist.

    Article Title: S-nitrosylation of fatty acid synthase regulates its activity through dimerization [S]
    Article Snippet: The NO donor, diethylamine diazeniumdiolate diethylammonium salt (DEA-NONOate) (D5431), and a NO synthase (NOS) inhibitor, Nω -nitro- L -arginine (N5501), were also obtained from Sigma-Aldrich.

    Article Title: Vascular endothelial dysfunction in Duchenne muscular dystrophy is restored by bradykinin through upregulation of eNOS and nNOS
    Article Snippet: Acetylcholine (Sigma), dissolved in H2 O; bradykinin (NeoMPS, Strasbourg, France), dissolved in saline solution; LNA, Nω-Nitro-L-arginine (Sigma), dissolved in H2 O; indomethacin (Sigma), dissolved in DMSO; U46619, 9,11-dideoxy-11α,9α-epoxymethanoprostaglandin F2α (Sigma), dissolved in methyl acetate.

    Article Title: Inhibitory neuromuscular transmission to ileal longitudinal muscle predominates in neonatal guinea pigs
    Article Snippet: Acetylcholine (A6625), nicotine (N5260), Nω -nitro-L-arginine (NLA; N5501) and tetrodotoxin (TTX; T5651) were all obtained from Sigma Chemical Company (St. Louis, MO).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Millipore n g nitro d arginine
    Endocannabinoid-stimulated NO accumulation antagonized by a NOS inhibitor. a N18TG2 cells were treated with a methanandamide (MetAEA) alone (1 μM) or b following pretreatment with NOS inhibitor <t>N</t> G -nitro- l -arginine ( l- NNA ; 1 μM; bar = 100 μm). b Quantitation of effects of NOS inhibitor l- NNA and its inactive enantiomer N G -nitro- d -arginine ( d -NNA ; 1 μM) on methanandamide-stimulated NO accumulation in N18TG2 cells. Data are presented as the DAF/DAPI fluorescence intensity ratio (mean ± SEM from N = 3 independent experiments). Values significantly different from those of MetAEA-stimulated cells (* p
    N G Nitro D Arginine, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n g nitro d arginine/product/Millipore
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    n g nitro d arginine - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Endocannabinoid-stimulated NO accumulation antagonized by a NOS inhibitor. a N18TG2 cells were treated with a methanandamide (MetAEA) alone (1 μM) or b following pretreatment with NOS inhibitor N G -nitro- l -arginine ( l- NNA ; 1 μM; bar = 100 μm). b Quantitation of effects of NOS inhibitor l- NNA and its inactive enantiomer N G -nitro- d -arginine ( d -NNA ; 1 μM) on methanandamide-stimulated NO accumulation in N18TG2 cells. Data are presented as the DAF/DAPI fluorescence intensity ratio (mean ± SEM from N = 3 independent experiments). Values significantly different from those of MetAEA-stimulated cells (* p

    Journal: Journal of Neuroimmune Pharmacology

    Article Title: Cannabinoid Regulation of Nitric Oxide Synthase I (nNOS) in Neuronal Cells

    doi: 10.1007/s11481-009-9153-7

    Figure Lengend Snippet: Endocannabinoid-stimulated NO accumulation antagonized by a NOS inhibitor. a N18TG2 cells were treated with a methanandamide (MetAEA) alone (1 μM) or b following pretreatment with NOS inhibitor N G -nitro- l -arginine ( l- NNA ; 1 μM; bar = 100 μm). b Quantitation of effects of NOS inhibitor l- NNA and its inactive enantiomer N G -nitro- d -arginine ( d -NNA ; 1 μM) on methanandamide-stimulated NO accumulation in N18TG2 cells. Data are presented as the DAF/DAPI fluorescence intensity ratio (mean ± SEM from N = 3 independent experiments). Values significantly different from those of MetAEA-stimulated cells (* p

    Article Snippet: N G -nitro-l -arginine (l- NNA) and N G -nitro-d -arginine (d -NNA) were purchased from EMD Chemicals (Gibbstown, NJ, USA).

    Techniques: Quantitation Assay, Fluorescence