n g monomethyl l arginine  (Millipore)


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    Structured Review

    Millipore n g monomethyl l arginine
    Endothelial NO production and TC adhesion along the same microvessel after 30 min perfusion under control ( A and B ) and under <t>N</t> G -monomethyl- l -arginine ( l -NMMA) treatment ( C and D ) under normal flow. The focus is on a focal plane for endothelial cells
    N G Monomethyl L Arginine, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n g monomethyl l arginine/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    n g monomethyl l arginine - by Bioz Stars, 2020-04
    93/100 stars

    Images

    1) Product Images from "Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows"

    Article Title: Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00109.2016

    Endothelial NO production and TC adhesion along the same microvessel after 30 min perfusion under control ( A and B ) and under N G -monomethyl- l -arginine ( l -NMMA) treatment ( C and D ) under normal flow. The focus is on a focal plane for endothelial cells
    Figure Legend Snippet: Endothelial NO production and TC adhesion along the same microvessel after 30 min perfusion under control ( A and B ) and under N G -monomethyl- l -arginine ( l -NMMA) treatment ( C and D ) under normal flow. The focus is on a focal plane for endothelial cells

    Techniques Used: Flow Cytometry

    2) Product Images from "Abnormal Remodeling of Subcutaneous Small Arteries Is Associated With Early Diastolic Impairment in Metabolic Syndrome"

    Article Title: Abnormal Remodeling of Subcutaneous Small Arteries Is Associated With Early Diastolic Impairment in Metabolic Syndrome

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.116.004603

    Assessment of endothelial function in patients with metabolic syndrome and controls. A, Effect of acetylcholine dilation on preconstricted small arteries from subcutaneous gluteal fat biopsy samples from control participants and patients with metabolic syndrome measured in a pressurized system. B, Acetylcholine relaxations after 1‐hour incubation with L‐ NMMA in control participants and those with metabolic syndrome. l ‐NMMA indicates N G ‐monomethyl‐ l ‐arginine.
    Figure Legend Snippet: Assessment of endothelial function in patients with metabolic syndrome and controls. A, Effect of acetylcholine dilation on preconstricted small arteries from subcutaneous gluteal fat biopsy samples from control participants and patients with metabolic syndrome measured in a pressurized system. B, Acetylcholine relaxations after 1‐hour incubation with L‐ NMMA in control participants and those with metabolic syndrome. l ‐NMMA indicates N G ‐monomethyl‐ l ‐arginine.

    Techniques Used: Incubation

    3) Product Images from "First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor"

    Article Title: First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor

    Journal: European Heart Journal

    doi: 10.1093/eurheartj/ehq156

    Study protocol. First, there were three 2-min infusions of acetylcholine (ACh) at 0.15, 1.5, and 15 µg/min via the infusion catheter positioned in the left anterior descending coronary artery. After a 5-min infusion of N G -monomethyl- l -arginine ( l -NMMA) at 40 µmol/min, ACh was infused at the three concentrations as before. Coronary arteriograms were recorded after each injection of ACh and l -NMMA. The coronary flow velocity of the left anterior descending coronary artery was continuously monitored during this protocol.
    Figure Legend Snippet: Study protocol. First, there were three 2-min infusions of acetylcholine (ACh) at 0.15, 1.5, and 15 µg/min via the infusion catheter positioned in the left anterior descending coronary artery. After a 5-min infusion of N G -monomethyl- l -arginine ( l -NMMA) at 40 µmol/min, ACh was infused at the three concentrations as before. Coronary arteriograms were recorded after each injection of ACh and l -NMMA. The coronary flow velocity of the left anterior descending coronary artery was continuously monitored during this protocol.

    Techniques Used: Injection, Flow Cytometry

    4) Product Images from "Exercise Training Prevents Arterial Baroreflex Dysfunction in Rats Treated With Central Angiotensin II"

    Article Title: Exercise Training Prevents Arterial Baroreflex Dysfunction in Rats Treated With Central Angiotensin II

    Journal: Hypertension

    doi: 10.1161/01.HYP.0000256955.74461.93

    Hypothalamic superoxide production in the basal state (A) or in the presence of NAD(P)H (10 μmol/L; B). Apocynin (1 mmol/L) and N G -monomethyl-L-arginine N G -monomethyl-L-arginine (1 mmol/L) were used to inhibit the activity of NAD(P)H oxidase and NOS, respectively. * P
    Figure Legend Snippet: Hypothalamic superoxide production in the basal state (A) or in the presence of NAD(P)H (10 μmol/L; B). Apocynin (1 mmol/L) and N G -monomethyl-L-arginine N G -monomethyl-L-arginine (1 mmol/L) were used to inhibit the activity of NAD(P)H oxidase and NOS, respectively. * P

    Techniques Used: Activity Assay

    5) Product Images from "Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate"

    Article Title: Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00195.2011

    Time-dependent changes in DAF fluorescence intensity with continuous DAF-2 DA perfusion at 1 ( A ) and 5 μM ( B ) in the absence and presence of N G -monomethyl- l -arginine ( l -NMMA). Two shaded areas indicate the transition from the dye loading phase
    Figure Legend Snippet: Time-dependent changes in DAF fluorescence intensity with continuous DAF-2 DA perfusion at 1 ( A ) and 5 μM ( B ) in the absence and presence of N G -monomethyl- l -arginine ( l -NMMA). Two shaded areas indicate the transition from the dye loading phase

    Techniques Used: Fluorescence

    Related Articles

    Concentration Assay:

    Article Title: First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor
    Article Snippet: Acetylcholine was infused into the LAD via the infusion catheter at 5 mL/min for 2 min in the following order: (i) three 2-min infusions of ACh at 0.15, 1.5, and 15 µg/min, yielding estimated intracoronary concentrations of 10−8 , 10−7 , and 10−6 M, respectively; (ii) a 5-min infusion of N G -monomethyl-l -arginine (l -NMMA) (Calbiochem) at 40 µmol/min; and (iii) three ACh infusions at the above three concentrations immediately after the l -NMMA infusion. .. Systemic arterial pressure, heart rate, 12-lead electrocardiogram, APV in the LAD, and changes in plasma NO concentration in the GCV were continuously measured throughout the study.

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: For infection, macrophages were washed, counted, and adjusted to a concentration of 2.5 × 106 cells/ml; 0.2 ml of BMMφ was added to each well of a Lab Tek eight-chamber slide (Nunc, Naperville, Ill.) and incubated for 2 h. After being washed three times with Hanks balanced salt solution (Gibco), BMMφ were infected with 50 μl of 2 × 107 parasites/ml, with a final ratio of two parasites to one BMMφ, and incubated for 2 h. Extracellular parasites were removed by rinsing gently with Hanks balanced salt solution. .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.).

    Article Title: Abnormal Remodeling of Subcutaneous Small Arteries Is Associated With Early Diastolic Impairment in Metabolic Syndrome
    Article Snippet: Pressure Myography: Pharmacological Assessment After viability assessment with KPSS, each vessel was stimulated as follows: (1) Cumulative addition of norepinephrine (Sigma‐Aldrich), 10−9 , 3×10−9 , 10−8 , 3×10−8 , 10−7 , 3×10−7 , 10−6 , 3×10−6 , 10−5 mol/L with 3 to 5 minutes incubation per concentration. (2) Endothelial function was assessed via the cumulative response to acetylcholine (Sigma‐Aldrich) achieved by adding serial concentrations (mol/L) 10−9 , 3×10−9 , 10−8 , 3×10−8 , 10−7 , 3×10−7 , 10−6 , 3×10−6 , 10−5 to a preconstricted vessel with 10−5 norepinephrine. .. After 1 hour of incubation with 5×10−5 mol/L N G ‐monomethyl‐l ‐arginine (Sigma), a NO synthase inhibitor, the responses to acetylcholine were repeated as in step 2 above.

    Infection:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: For infection, macrophages were washed, counted, and adjusted to a concentration of 2.5 × 106 cells/ml; 0.2 ml of BMMφ was added to each well of a Lab Tek eight-chamber slide (Nunc, Naperville, Ill.) and incubated for 2 h. After being washed three times with Hanks balanced salt solution (Gibco), BMMφ were infected with 50 μl of 2 × 107 parasites/ml, with a final ratio of two parasites to one BMMφ, and incubated for 2 h. Extracellular parasites were removed by rinsing gently with Hanks balanced salt solution. .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.).

    Article Title: CD40 and tumour necrosis factor-? co-operate to up-regulate inducuble nitric oxide synthase expression in macrophages
    Article Snippet: Paragraph title: Leishmania major infection ... In certain experiments macrophages were incubated with N G -monomethyl- l -arginine (NMA, 100 μ m ; Calbiochem, San Diego, CA) before CD40/TNF-α or IFN-γ/TNF-α stimulation.

    Mouse Assay:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: Bone marrow cells from IFN-γR−/− , iNOS−/− , and wild-type mice were flushed from mouse femora and cultivated at a concentration of 5 × 105 cells per ml in hydrophobic Teflon film bags (Hereaus, Hanau, Germany) as previously described ( ). .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.).

    Article Title: CD40 and tumour necrosis factor-? co-operate to up-regulate inducuble nitric oxide synthase expression in macrophages
    Article Snippet: Leishmania major (World Health Organization strain WHOM/IR/-/173) was maintained by infecting footpads of BALB/c mice. .. In certain experiments macrophages were incubated with N G -monomethyl- l -arginine (NMA, 100 μ m ; Calbiochem, San Diego, CA) before CD40/TNF-α or IFN-γ/TNF-α stimulation.

    Light Microscopy:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.). .. Two hundred fifty cells per duplicate well were counted by light microscopy on Diff Quick (Baxter Scientific, Mundelein, Ill.)-stained slides.

    Article Title: CD40 and tumour necrosis factor-? co-operate to up-regulate inducuble nitric oxide synthase expression in macrophages
    Article Snippet: In certain experiments macrophages were incubated with N G -monomethyl- l -arginine (NMA, 100 μ m ; Calbiochem, San Diego, CA) before CD40/TNF-α or IFN-γ/TNF-α stimulation. .. Parasite replication was assessed by light microscopy.

    Diff-Quik:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.). .. Two hundred fifty cells per duplicate well were counted by light microscopy on Diff Quick (Baxter Scientific, Mundelein, Ill.)-stained slides.

    Incubation:

    Article Title: ?-Galactosylceramide Promotes Killing of Listeria monocytogenes within the Macrophage Phagosome through Invariant NKT-Cell Activation ▿
    Article Snippet: .. Mφ were incubated in 24-well tissue culture plates (Corning) at 106 /well in the presence or absence of N G -monomethyl- l -arginine (500 μM; Calbiochem, Darmstadt, Germany) or anti-IFN-γ neutralizing MAb (50 μg/ml) for 24 h. .. TCRVα14 mRNA expression was analyzed as described previously , with slight modifications.

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.). .. Two hundred fifty cells per duplicate well were counted by light microscopy on Diff Quick (Baxter Scientific, Mundelein, Ill.)-stained slides.

    Article Title: CD40 and tumour necrosis factor-? co-operate to up-regulate inducuble nitric oxide synthase expression in macrophages
    Article Snippet: .. In certain experiments macrophages were incubated with N G -monomethyl- l -arginine (NMA, 100 μ m ; Calbiochem, San Diego, CA) before CD40/TNF-α or IFN-γ/TNF-α stimulation. .. Parasite replication was assessed by light microscopy.

    Article Title: Abnormal Remodeling of Subcutaneous Small Arteries Is Associated With Early Diastolic Impairment in Metabolic Syndrome
    Article Snippet: .. After 1 hour of incubation with 5×10−5 mol/L N G ‐monomethyl‐l ‐arginine (Sigma), a NO synthase inhibitor, the responses to acetylcholine were repeated as in step 2 above. ..

    Inhibition:

    Article Title: ?-Galactosylceramide Promotes Killing of Listeria monocytogenes within the Macrophage Phagosome through Invariant NKT-Cell Activation ▿
    Article Snippet: Paragraph title: Inhibition experiment. ... Mφ were incubated in 24-well tissue culture plates (Corning) at 106 /well in the presence or absence of N G -monomethyl- l -arginine (500 μM; Calbiochem, Darmstadt, Germany) or anti-IFN-γ neutralizing MAb (50 μg/ml) for 24 h.

    Activity Assay:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: The culture medium contained 70% high-glucose-formulation Dulbecco’s modified Eagle’s Medium (Gibco), supplemented with 2 mM l -glutamine, 0.01 mM sodium pyruvate, 5% heat-inactivated horse serum, 10% heat-inactivated fetal calf serum (Gibco), 0.05 mM 2-mercaptoethanol (Roth), penicillin and streptomycin (100 U/ml and 100 μg/ml, respectively) (Biochrom), and 30% L929 conditioned medium, as a source of macrophage colony-stimulating factor activity. .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.).

    Cell Culture:

    Article Title: CD40 and tumour necrosis factor-? co-operate to up-regulate inducuble nitric oxide synthase expression in macrophages
    Article Snippet: Metacyclic promastigotes were incubated with macrophages cultured on eight-chamber tissue culture glass slides (Falcon; BD Biosciences, San Jose, CA; 1 × 105 cells/chamber) at a ratio of six parasites/macrophage. .. In certain experiments macrophages were incubated with N G -monomethyl- l -arginine (NMA, 100 μ m ; Calbiochem, San Diego, CA) before CD40/TNF-α or IFN-γ/TNF-α stimulation.

    Modification:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: The culture medium contained 70% high-glucose-formulation Dulbecco’s modified Eagle’s Medium (Gibco), supplemented with 2 mM l -glutamine, 0.01 mM sodium pyruvate, 5% heat-inactivated horse serum, 10% heat-inactivated fetal calf serum (Gibco), 0.05 mM 2-mercaptoethanol (Roth), penicillin and streptomycin (100 U/ml and 100 μg/ml, respectively) (Biochrom), and 30% L929 conditioned medium, as a source of macrophage colony-stimulating factor activity. .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.).

    Recombinant:

    Article Title: Defective Nitric Oxide Effector Functions Lead to Extreme Susceptibility of Trypanosoma cruzi-Infected Mice Deficient in Gamma Interferon Receptor or Inducible Nitric Oxide Synthase
    Article Snippet: .. Intracellular parasite elimination was determined after a 2-day incubation with complete media, supplemented with recombinant murine IFN-γ (100 or 10 U/ml), TNF-α (50 U/ml; Pharmingen, San Diego, Calif.) (the endotoxin level is < 0.1 ng per mg), N G -monomethyl- l -arginine ( l -NMMA; 500 μM) (Calbiochem, San Diego, Calif.), and anti-IFN-γ (500 ng/ml) (R4-6A2; ATCC) or lipopolysaccharide (LPS) (10 μg/ml; Sigma, St. Louis, Mo.). .. Two hundred fifty cells per duplicate well were counted by light microscopy on Diff Quick (Baxter Scientific, Mundelein, Ill.)-stained slides.

    Article Title: CD40 and tumour necrosis factor-? co-operate to up-regulate inducuble nitric oxide synthase expression in macrophages
    Article Snippet: Thereafter, macrophages were incubated with complete medium containing either a stimulatory anti-CD40 mAb or control mAb, recombinant mouse CD154, recombinant mouse TNF-α, recombinant mouse IFN-γ or LPS ( Escherichia coli O55:B5; 100 ng/ml; Sigma Chemical). .. In certain experiments macrophages were incubated with N G -monomethyl- l -arginine (NMA, 100 μ m ; Calbiochem, San Diego, CA) before CD40/TNF-α or IFN-γ/TNF-α stimulation.

    other:

    Article Title: ACh-induced rebound stimulation of L-type Ca2+ current in guinea-pig ventricular myocytes, mediated by G??-dependent activation of adenylyl cyclase
    Article Snippet: Acetylcholine hydrochloride (ACh), R(-)-isoprenaline (+)-bitartrate (Iso), histamine hydrochloride (Sigma), N G -monomethyl- l -arginine ( l -NMMA), cholera toxin (Calbiochem) and pertussis toxin (PTX, List Biochemical Laboratories) were prepared as aqueous stock solutions.

    Article Title: Roles of Rho-Associated Kinase and Oxidative Stress in the Pathogenesis of Aortic Stiffness
    Article Snippet: Forearm vascular response to sodium nitroprusside (SNP) (Maluishi Pharmaceutical Co.) alone, acetylcholine (ACh) (Daiichi Pharmaceutical Co.) alone, and fasudil (Asahi Chemical Industries) alone and after coinfusion of N G -monomethyl-L-arginine (L-NMMA) (Sigma Chemical Co.), were evaluated.

    Article Title: Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate
    Article Snippet: DAF-2 DA, DAF-2, N G -monomethyl- l -arginine ( l -NMMA), 1-oalkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF), and sodium nitroprusside were all purchased from Sigma.

    Article Title: Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows
    Article Snippet: FITC-labeled BSA (molecular mass ∼67 kDa; A9771), 4,5-diaminofluorescein diacetate (DAF-2 DA), N G -monomethyl- l -arginine ( l -NMMA), and sodium nitroprusside (SNP) were purchased from Sigma (Sigma-Aldrich).

    Article Title: Exercise Training Prevents Arterial Baroreflex Dysfunction in Rats Treated With Central Angiotensin II
    Article Snippet: Apocynin and N G -monomethyl-L-arginine were obtained from the Calbiochem Co. Losartan was a gift from Merck and Co. Ketamine was obtained from Ft Dodge Laboratories Inc. Nitroglycerin was obtained from ETHEX Co. Ang II and other chemicals were obtained from Sigma-Aldrich Co.

    Article Title: Relaxant effect of all-trans-retinoic acid via NO-sGC-cGMP pathway and calcium-activated potassium channels in rat mesenteric artery
    Article Snippet: ATRA, ACh chloride, glibenclamide, 4-aminopyridine (4-AP), charybdotoxin, Phe HCl, l -NAME, S -methylisothiourea sulfate (SMT), N G -monomethyl- l -arginine, 1H-[1,2,4]-oxadiazole-[4,3-α]-quinoxaline-1-one (ODQ), and DAF-2DA were obtained from Sigma-Aldrich (St. Louis, MO).

    Article Title: LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection
    Article Snippet: Rapamycin, tyrphostin AG126, Bafilomycin A1 , chloroquine, N G -monomethyl- l -arginine ( l -NMMA), and sodium nitroprusside (SNP) were purchased from EMD Biosciences.

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    Millipore no synthase
    Combination of lipopolysaccharide (LPS) and interferon (IFN)-γ upregulates cyclooxygenase (COX)-2, inducible nitric oxide (NO) <t>synthase</t> (iNOS), and nitrite production. A : rat aortic smooth muscle cells (SMCs) were exposed to LPS ± IFN-γ for 4 h, lysed, and probed for COX-2, iNOS, or actin by Western blot analysis. B : media collected from SMCs was analyzed for nitrite accumulation as measured by the Griess assay and normalized to protein. C and D : time course (0–240 min) of COX-1 and COX-2 expression following exposure of SMCs to LPS + IFN-γ. Shown are representative blots [ n = 4 ( A and B ) and n = 3 ( C and D )], and the ratio of protein of interest to actin control was expressed following densitometric analysis. Data are means ± SE. * P ≤ 0.05 vs. control or zero time.
    No Synthase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/no synthase/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    no synthase - by Bioz Stars, 2020-04
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    94
    Millipore l nmma
    Effect of autocrine NOS2-derived NO on γδ T cell proliferation. γδ T cells sorted from pLNs of WT or Nos2KO mice and labeled with CFSE were cultured for 2 days in presence of CD3- and CD28-specific antibodies, 0,5 mM <t>L-NIL</t> and 10 mM <t>L-NMMA</t> when indicated. (A) Representative histograms of CFSE dilution. Numbers above line on CFSE plots indicate percent of proliferating cells (left). Percentages of γδ T cell proliferation undergoing division (right). (B) Number of γδ T cells by division. (C) IL-2 levels quantified in supernatants by ELISA after 24h and 48h of γδ T cell cultures. (D) Proportion of living γδ T cells after 48h culture. Data are from five independent experiments with 12 WT, 5 Nos2KO, 3 L-NIL and 6 L-NMMA replicates. Point represents individual replicate, bars mean ± SEM (except in B right mean) * p
    L Nmma, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l nmma/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    91
    Millipore inducible nitric oxide synthase inos inhibitor ng monomethyl l arginine
    Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> inhibitor N G <t>-monomethyl_L-arginine</t> (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).
    Inducible Nitric Oxide Synthase Inos Inhibitor Ng Monomethyl L Arginine, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inducible nitric oxide synthase inos inhibitor ng monomethyl l arginine/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    inducible nitric oxide synthase inos inhibitor ng monomethyl l arginine - by Bioz Stars, 2020-04
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    Combination of lipopolysaccharide (LPS) and interferon (IFN)-γ upregulates cyclooxygenase (COX)-2, inducible nitric oxide (NO) synthase (iNOS), and nitrite production. A : rat aortic smooth muscle cells (SMCs) were exposed to LPS ± IFN-γ for 4 h, lysed, and probed for COX-2, iNOS, or actin by Western blot analysis. B : media collected from SMCs was analyzed for nitrite accumulation as measured by the Griess assay and normalized to protein. C and D : time course (0–240 min) of COX-1 and COX-2 expression following exposure of SMCs to LPS + IFN-γ. Shown are representative blots [ n = 4 ( A and B ) and n = 3 ( C and D )], and the ratio of protein of interest to actin control was expressed following densitometric analysis. Data are means ± SE. * P ≤ 0.05 vs. control or zero time.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Inducible nitric oxide synthase gene deletion exaggerates MAPK-mediated cyclooxygenase-2 induction by inflammatory stimuli

    doi: 10.1152/ajpheart.00144.2010

    Figure Lengend Snippet: Combination of lipopolysaccharide (LPS) and interferon (IFN)-γ upregulates cyclooxygenase (COX)-2, inducible nitric oxide (NO) synthase (iNOS), and nitrite production. A : rat aortic smooth muscle cells (SMCs) were exposed to LPS ± IFN-γ for 4 h, lysed, and probed for COX-2, iNOS, or actin by Western blot analysis. B : media collected from SMCs was analyzed for nitrite accumulation as measured by the Griess assay and normalized to protein. C and D : time course (0–240 min) of COX-1 and COX-2 expression following exposure of SMCs to LPS + IFN-γ. Shown are representative blots [ n = 4 ( A and B ) and n = 3 ( C and D )], and the ratio of protein of interest to actin control was expressed following densitometric analysis. Data are means ± SE. * P ≤ 0.05 vs. control or zero time.

    Article Snippet: In an effort to induce both COX-2 and iNOS, SMCs were exposed to the combination of proinflammatory stimuli lipopolysaccharide (LPS) and interferon (IFN)-γ for 0–240 min. Because of assay sensitivity constraints and characteristic lag phase associated with iNOS induction and nitrite production, the cells for these measurements were exposed to stimuli for 4 h. Where noted, the cells were pretreated for 30 min with pharmacological inhibitors of NO synthase [ N G -monomethyl- l -arginine ( l -NMMA); 0.01–5 mmol/l], ERK (U-0126; 10 μmol/l), p38 (SB-202190; 30 μmol/l), JNK (SP-600125; 10 μmol/l; Calbiochem), or guanylate cyclase {1H-[1,2,4] oxadiazolo[4,3-a]quinoxalin-1-one (ODQ); 10 μmol/l}.

    Techniques: Western Blot, Griess Assay, Expressing

    Effect of autocrine NOS2-derived NO on γδ T cell proliferation. γδ T cells sorted from pLNs of WT or Nos2KO mice and labeled with CFSE were cultured for 2 days in presence of CD3- and CD28-specific antibodies, 0,5 mM L-NIL and 10 mM L-NMMA when indicated. (A) Representative histograms of CFSE dilution. Numbers above line on CFSE plots indicate percent of proliferating cells (left). Percentages of γδ T cell proliferation undergoing division (right). (B) Number of γδ T cells by division. (C) IL-2 levels quantified in supernatants by ELISA after 24h and 48h of γδ T cell cultures. (D) Proportion of living γδ T cells after 48h culture. Data are from five independent experiments with 12 WT, 5 Nos2KO, 3 L-NIL and 6 L-NMMA replicates. Point represents individual replicate, bars mean ± SEM (except in B right mean) * p

    Journal: PLoS ONE

    Article Title: Nitric Oxide Synthase 2 Improves Proliferation and Glycolysis of Peripheral γδ T Cells

    doi: 10.1371/journal.pone.0165639

    Figure Lengend Snippet: Effect of autocrine NOS2-derived NO on γδ T cell proliferation. γδ T cells sorted from pLNs of WT or Nos2KO mice and labeled with CFSE were cultured for 2 days in presence of CD3- and CD28-specific antibodies, 0,5 mM L-NIL and 10 mM L-NMMA when indicated. (A) Representative histograms of CFSE dilution. Numbers above line on CFSE plots indicate percent of proliferating cells (left). Percentages of γδ T cell proliferation undergoing division (right). (B) Number of γδ T cells by division. (C) IL-2 levels quantified in supernatants by ELISA after 24h and 48h of γδ T cell cultures. (D) Proportion of living γδ T cells after 48h culture. Data are from five independent experiments with 12 WT, 5 Nos2KO, 3 L-NIL and 6 L-NMMA replicates. Point represents individual replicate, bars mean ± SEM (except in B right mean) * p

    Article Snippet: When indicated 15 U ml-1 rIL-2 (R & D Systems) or 10 mM L-NMMA (Calbiochem), 0.5 mM N6-(1-iminoethyl)-l-lysine dihydrochloride (L-NIL, Calbiochem) were added to the cultures.

    Techniques: Derivative Assay, Mouse Assay, Labeling, Cell Culture, Enzyme-linked Immunosorbent Assay

    Effect of 20-HETE on NO production (A), superoxide generation (B), and cGMP production (C) in endothelial cells. Cells were preincubated with and without l -NMMA (0.1 mM) and 20-HETE (5 nM) for 30 min, followed by incubation with the calcium ionophore

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: 20-Hydroxy-5,8,11,14-eicosatetraenoic Acid Mediates Endothelial Dysfunction via I?B Kinase-Dependent Endothelial Nitric-Oxide Synthase Uncoupling

    doi: 10.1124/jpet.109.159863

    Figure Lengend Snippet: Effect of 20-HETE on NO production (A), superoxide generation (B), and cGMP production (C) in endothelial cells. Cells were preincubated with and without l -NMMA (0.1 mM) and 20-HETE (5 nM) for 30 min, followed by incubation with the calcium ionophore

    Article Snippet: Cells were cultured on 96-well plates until they achieved ∼70% confluence and were placed in FBS-free medium for 24 h. After treatments with or without 4,5-dihydroxy-1,3-benzene disulfonic acid (tiron; 10 mM), l -NMMA (0.1 mM), 20-HETE (5 nM), calcium ionophore A23187 (5 μM), and l -arginine (25 μM) for 30 min, the cells were incubated with 10 μM dihydroethidium (superoxide; Calbiochem) for 30 min at 37°C.

    Techniques: Incubation

    Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide synthase (iNOS) inhibitor N G -monomethyl_L-arginine (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).

    Journal: Cellular immunology

    Article Title: Macrophage regulation of B cell proliferation

    doi: 10.1016/j.cellimm.2017.02.002

    Figure Lengend Snippet: Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide synthase (iNOS) inhibitor N G -monomethyl_L-arginine (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).

    Article Snippet: To inhibit arginine catabolism, the arginase (ARG) inhibitor N-ω -hydroxy-nor-L-arginine (1-NA; CalBiochem, San Diego, CA) or the inducible nitric oxide synthase (iNOS) inhibitor NG -monomethyl-L-arginine (1-MA; CalBiochem) were added.

    Techniques: Ligation, Cell Culture