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Proteintech n-cadherin
N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n-cadherin/product/Proteintech
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n-cadherin - by Bioz Stars, 2024-06
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Proteintech n cadherin proteintech 22018 1 ap
N Cadherin Proteintech 22018 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin proteintech 22018 1 ap/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin proteintech 22018 1 ap - by Bioz Stars, 2024-06
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Proteintech n cadherin proteintech
Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, <t>E-cadherin,</t> N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.
N Cadherin Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin proteintech/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin proteintech - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Circ_0007386 Promotes the Progression of Hepatocellular Carcinoma Through the miR-507/ CCNT2 Axis"

Article Title: Circ_0007386 Promotes the Progression of Hepatocellular Carcinoma Through the miR-507/ CCNT2 Axis

Journal: Journal of Hepatocellular Carcinoma

doi: 10.2147/JHC.S459633

Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.
Figure Legend Snippet: Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.

Techniques Used: In Vivo, Immunohistochemical staining, Expressing, Over Expression, Staining


Structured Review

Proteintech n cadherin proteintech 22018 1 ap
N Cadherin Proteintech 22018 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin proteintech 22018 1 ap/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin proteintech 22018 1 ap - by Bioz Stars, 2024-06
86/100 stars

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Structured Review

Proteintech anti n cadherin
Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, <t>E-cadherin,</t> N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.
Anti N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti n cadherin/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti n cadherin - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Circ_0007386 Promotes the Progression of Hepatocellular Carcinoma Through the miR-507/ CCNT2 Axis"

Article Title: Circ_0007386 Promotes the Progression of Hepatocellular Carcinoma Through the miR-507/ CCNT2 Axis

Journal: Journal of Hepatocellular Carcinoma

doi: 10.2147/JHC.S459633

Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.
Figure Legend Snippet: Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.

Techniques Used: In Vivo, Immunohistochemical staining, Expressing, Over Expression, Staining


Structured Review

Proteintech n cadherin
PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, <t>E-cadherin</t> and Vimentin
N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "OLFM4 promotes the progression of intestinal metaplasia through activation of the MYH9/GSK3β/β-catenin pathway"

Article Title: OLFM4 promotes the progression of intestinal metaplasia through activation of the MYH9/GSK3β/β-catenin pathway

Journal: Molecular Cancer

doi: 10.1186/s12943-024-02016-9

PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, E-cadherin and Vimentin
Figure Legend Snippet: PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, E-cadherin and Vimentin

Techniques Used: Expressing, Activation Assay, RNA Sequencing Assay, Western Blot, Staining, Immunofluorescence


Structured Review

Proteintech n cadherin polyclonal antibody
N Cadherin Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin polyclonal antibody/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin polyclonal antibody - by Bioz Stars, 2024-06
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Structured Review

Proteintech anti n cadherin
Anti N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti n cadherin/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti n cadherin - by Bioz Stars, 2024-06
86/100 stars

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Structured Review

Proteintech n cadherin
Effects of POLR1D silencing on proteins of EMT and PI3K-AKT pathway. a Representative gel blots depicting levels of cleaved caspase-3 (normalized to GAPDH), <t>E-cadherin</t> (normalized to GAPDH), N-cadherin (normalized to GAPDH), phosphorylated PI3K (p-PI3K) and total PI3K (normalized to total PI3K), phosphorylated AKT (p-AKT) and total AKT (normalized to total AKT). These protein blots were quantified in ( b ) SK-MES-1 cells and c H2170 cells. Data represent the average of three independent experiments (mean ± SD). ** P < 0.01, *** P < 0.001 vs. PBS group
N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "POLR1D silencing suppresses lung cancer cells proliferation and migration via inhibition of PI3K-Akt pathway"

Article Title: POLR1D silencing suppresses lung cancer cells proliferation and migration via inhibition of PI3K-Akt pathway

Journal: Journal of Cardiothoracic Surgery

doi: 10.1186/s13019-024-02791-y

Effects of POLR1D silencing on proteins of EMT and PI3K-AKT pathway. a Representative gel blots depicting levels of cleaved caspase-3 (normalized to GAPDH), E-cadherin (normalized to GAPDH), N-cadherin (normalized to GAPDH), phosphorylated PI3K (p-PI3K) and total PI3K (normalized to total PI3K), phosphorylated AKT (p-AKT) and total AKT (normalized to total AKT). These protein blots were quantified in ( b ) SK-MES-1 cells and c H2170 cells. Data represent the average of three independent experiments (mean ± SD). ** P < 0.01, *** P < 0.001 vs. PBS group
Figure Legend Snippet: Effects of POLR1D silencing on proteins of EMT and PI3K-AKT pathway. a Representative gel blots depicting levels of cleaved caspase-3 (normalized to GAPDH), E-cadherin (normalized to GAPDH), N-cadherin (normalized to GAPDH), phosphorylated PI3K (p-PI3K) and total PI3K (normalized to total PI3K), phosphorylated AKT (p-AKT) and total AKT (normalized to total AKT). These protein blots were quantified in ( b ) SK-MES-1 cells and c H2170 cells. Data represent the average of three independent experiments (mean ± SD). ** P < 0.01, *** P < 0.001 vs. PBS group

Techniques Used:


Structured Review

Proteintech n cadherin
SULF1 knockdown inhibits EMT in CRC cells through the FAK/PI3K/AKT/mTor pathway. (A) GO and KEGG enrichment analyses showed that SULF1 was involved in CRC progression via the FAK/PI3K/AKT/mTor pathway. (B) Western blotting showed that SULF1 knockdown could decrease the protein expression of p-FAK, p-PI3K, p-AKT, and p-mTor. (C) Western blotting showed that SULF1 knockdown could decrease the protein expression of EMT markers, <t>N-cadherin</t> and Snail, but increase E-cadherin. All experiments were repeated three times and the data are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n cadherin/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
n cadherin - by Bioz Stars, 2024-06
86/100 stars

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1) Product Images from "SULF1 regulates malignant progression of colorectal cancer by modulating ARSH via FAK/PI3K/AKT/mTOR signaling"

Article Title: SULF1 regulates malignant progression of colorectal cancer by modulating ARSH via FAK/PI3K/AKT/mTOR signaling

Journal: Cancer Cell International

doi: 10.1186/s12935-024-03383-5

SULF1 knockdown inhibits EMT in CRC cells through the FAK/PI3K/AKT/mTor pathway. (A) GO and KEGG enrichment analyses showed that SULF1 was involved in CRC progression via the FAK/PI3K/AKT/mTor pathway. (B) Western blotting showed that SULF1 knockdown could decrease the protein expression of p-FAK, p-PI3K, p-AKT, and p-mTor. (C) Western blotting showed that SULF1 knockdown could decrease the protein expression of EMT markers, N-cadherin and Snail, but increase E-cadherin. All experiments were repeated three times and the data are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Figure Legend Snippet: SULF1 knockdown inhibits EMT in CRC cells through the FAK/PI3K/AKT/mTor pathway. (A) GO and KEGG enrichment analyses showed that SULF1 was involved in CRC progression via the FAK/PI3K/AKT/mTor pathway. (B) Western blotting showed that SULF1 knockdown could decrease the protein expression of p-FAK, p-PI3K, p-AKT, and p-mTor. (C) Western blotting showed that SULF1 knockdown could decrease the protein expression of EMT markers, N-cadherin and Snail, but increase E-cadherin. All experiments were repeated three times and the data are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques Used: Western Blot, Expressing

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    Proteintech n-cadherin
    N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n-cadherin/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n-cadherin - by Bioz Stars, 2024-06
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    86
    Proteintech n cadherin proteintech 22018 1 ap
    N Cadherin Proteintech 22018 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin proteintech 22018 1 ap/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n cadherin proteintech 22018 1 ap - by Bioz Stars, 2024-06
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    86
    Proteintech n cadherin proteintech
    Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, <t>E-cadherin,</t> N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.
    N Cadherin Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin proteintech/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n cadherin proteintech - by Bioz Stars, 2024-06
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    Proteintech anti n cadherin
    Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, <t>E-cadherin,</t> N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.
    Anti N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti n cadherin/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti n cadherin - by Bioz Stars, 2024-06
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    Proteintech n cadherin
    PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, <t>E-cadherin</t> and Vimentin
    N Cadherin, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n cadherin - by Bioz Stars, 2024-06
    86/100 stars
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    86
    Proteintech n cadherin polyclonal antibody
    PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, <t>E-cadherin</t> and Vimentin
    N Cadherin Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n cadherin polyclonal antibody - by Bioz Stars, 2024-06
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    Image Search Results


    Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Circ_0007386 Promotes the Progression of Hepatocellular Carcinoma Through the miR-507/ CCNT2 Axis

    doi: 10.2147/JHC.S459633

    Figure Lengend Snippet: Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.

    Article Snippet: The primary antibodies used in this experiment were as follows: GAPDH (Proteintech, Wuhan, China), E-cadherin (Proteintech), N-cadherin (Proteintech), vimentin (Servicebio), and CCNT2 (Bioss, Beijing).

    Techniques: In Vivo, Immunohistochemical staining, Expressing, Over Expression, Staining

    Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Circ_0007386 Promotes the Progression of Hepatocellular Carcinoma Through the miR-507/ CCNT2 Axis

    doi: 10.2147/JHC.S459633

    Figure Lengend Snippet: Circ_0007386 promotes the proliferation and metastasis of HCC cells in vivo. ( A ) Images of subcutaneous xenograft tumors (6 mice per group) in nude mice. The tumor volume and average weight were measured. ( B ) Immunohistochemical verification of the expression of HE, Ki67, E-cadherin, N-cadherin and Vimentin in tumors tissues. ( C ) Circ_0007386 stable knockdown or overexpression cells were used to generate lung metastasis. Lung metastatic nodules were stained with HE. The number of metastases was calculated. Data were all showed as mean ± SD; ns indicated no significance, **p < 0.01, ***p < 0.001.

    Article Snippet: The cells were then blocked with goat serum for 30 minutes at 37 °C, and treated overnight at 4 °C with primary anti-E-cadherin (Proteintech), anti-N-cadherin (Proteintech), and anti-Vimentin (Servicebio) antibodies.

    Techniques: In Vivo, Immunohistochemical staining, Expressing, Over Expression, Staining

    PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, E-cadherin and Vimentin

    Journal: Molecular Cancer

    Article Title: OLFM4 promotes the progression of intestinal metaplasia through activation of the MYH9/GSK3β/β-catenin pathway

    doi: 10.1186/s12943-024-02016-9

    Figure Lengend Snippet: PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c - d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e - f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, E-cadherin and Vimentin

    Article Snippet: The primary antibodies included OLFM4 (14369S, CST, 1:1000), MYH9 (11128–1-AP, Proteintech, 1:5000), GSK3-β (22104–1-AP, Proteintech, 1:1000), β-catenin (51067–2-AP, Proteintech, 1:5000), β-actin (66009–1-Ig, Proteintech, 1:20000), p-STAT3 (9145S, CST, 1:2000), c-Myc (10828–1-AP, Proteintech, 1:2000), N-cadherin (22018–1-AP, Proteintech, 1:2000), E-cadherin (60335–1-Ig, Proteintech, 1:2000), Vimentin (60330–1-Ig, Proteintech, 1:20000), Snai1 (13099–1-AP, Proteintech, 1:500), Ubiquitin (10201–2-AP, Proteintech, 1:1000), and GAPDH (60004–1-Ig, Proteintech, 1:50000).

    Techniques: Expressing, Activation Assay, RNA Sequencing Assay, Western Blot, Staining, Immunofluorescence