n 7413 trilink  (TriLink)

 
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    TriLink n 7413 trilink
    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 <t>(CC1)</t> capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    N 7413 Trilink, supplied by TriLink, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 7413 trilink/product/TriLink
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n 7413 trilink - by Bioz Stars, 2023-06
    86/100 stars

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    1) Product Images from "Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA"

    Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics14020328

    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 (CC1) capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    Figure Legend Snippet: Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 (CC1) capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.

    Techniques Used: Purification

    LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.
    Figure Legend Snippet: LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Purification

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    TriLink n 7413 trilink
    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 <t>(CC1)</t> capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    N 7413 Trilink, supplied by TriLink, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 7413 trilink/product/TriLink
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n 7413 trilink - by Bioz Stars, 2023-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 (CC1) capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.

    Journal: Pharmaceutics

    Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

    doi: 10.3390/pharmaceutics14020328

    Figure Lengend Snippet: Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 (CC1) capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.

    Article Snippet: UPLC and MS profiles of CleanCap ® Reagent AG (3′ OMe), N-7413 TriLink (CC1) EPO mRNA ( ) showed >99% capping, which is also in agreement with >90% capping efficiency obtained by screening of >100 CC1-capped IVT mRNAs using 21% PAGE, 8 M urea ( and data not shown).

    Techniques: Purification

    LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

    Journal: Pharmaceutics

    Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

    doi: 10.3390/pharmaceutics14020328

    Figure Lengend Snippet: LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

    Article Snippet: UPLC and MS profiles of CleanCap ® Reagent AG (3′ OMe), N-7413 TriLink (CC1) EPO mRNA ( ) showed >99% capping, which is also in agreement with >90% capping efficiency obtained by screening of >100 CC1-capped IVT mRNAs using 21% PAGE, 8 M urea ( and data not shown).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification