n 6 biotinamido hexyl  (Thermo Fisher)


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    Name:
    Biotin X SE 6 Biotinoyl Amino Hexanoic Acid Succinimidyl Ester Biotinamidocaproate N Hydroxysuccinimidyl Ester
    Description:
    The amine reactive biotin X SE can be used to attach this important hapten to biomolecules of interest for subsequent detection with streptavidin avidin or NeutrAvidin biotin binding protein
    Catalog Number:
    b1582
    Price:
    None
    Applications:
    Amine-Reactive Biotins & Haptens|Cell Analysis|Labeling Amines|Labeling Proteins & Antibodies with Biotin & Other Haptens|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein and Antibody Labeling|Protein, Peptide & Antibody Labeling|Labeling Chemistry
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher n 6 biotinamido hexyl
    The amine reactive biotin X SE can be used to attach this important hapten to biomolecules of interest for subsequent detection with streptavidin avidin or NeutrAvidin biotin binding protein
    https://www.bioz.com/result/n 6 biotinamido hexyl/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n 6 biotinamido hexyl - by Bioz Stars, 2020-09
    98/100 stars

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    bis sulfosuccinimidyl suberate

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    Modification:

    Article Title: miR302a inhibits the proliferation of esophageal cancer cells through the MAPK and PI3K/Akt signaling pathways
    Article Snippet: .. JN-B1582; ), TE-11 (cat. no. JN-A2623; ), ECA109 (cat. no. JN-B1684; ) and Het-1A (cat. no. JN-4897; ) cells were grown in Dulbecco's modified Eagle's medium (DMEM) medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), supplemented with 10% fetal bovine serum (FBS; Hangzhou Sijiqing Biology Engineering Materials Co., Ltd., Hangzhou, China). .. Cells were cultured at 37°C in a 5% CO2 atmosphere.

    Immunoprecipitation:

    Article Title: Dauricine Protects from LPS-Induced Bone Loss via the ROS/PP2A/NF-κB Axis in Osteoclasts
    Article Snippet: .. The lysate was subjected to immunoprecipitation with 1 µg of PP2A-specific Ab, and HRP-conjugated streptavidin was used to detect immunocomplexes labeled with N-(biotinoyl)-N’-(iodoacetyl) ethylenediamine (BIAM) (Invitrogen) for development with an enhanced chemiluminescence kit. .. Transfection of siRNASmall interfering RNA (siRNA) against PP2A (a pool of 3 target-specific 19–25 nt siRNAs designed to knock down gene expression) or scrambled siRNA (scRNA) was transfected into pre-OCs that had previously been incubated with M-CSF and RANKL for 40 h using Lipofectamine 3000 as described [ ].

    Incubation:

    Article Title: Comparative Proteomic Analysis of Cysteine Oxidation in Colorectal Cancer Patients
    Article Snippet: .. To assess patterns of oxidation, samples (∼50 mg) of frozen tumor tissue and tissue from non-tumor regions were homogenized in 500 μl of chilled labeling buffer [20 mM Tris-HCl (pH 7.5), 100 mM NaCl 1 mM EDTA, 0.1 mM AEBSF, leupeptin (2.0 μg/ml), and aprotinin (1.9 μg/ml)] containing 40 μM N-(biotinoyl)-N’-(iodoacetyl) ethylenediamine (biotinylated iodoacetamide, BIAM) (Molecular Probes, USA) and incubated for 4 h at 4°C. .. The labeling reaction was quenched by adding 4 mM dithiothreitol (DTT), after which the mixtures were centrifuged at 5, 000 rpm for 30 min at 4°C, and the supernatant were discarded.

    Labeling:

    Article Title: Dauricine Protects from LPS-Induced Bone Loss via the ROS/PP2A/NF-κB Axis in Osteoclasts
    Article Snippet: .. The lysate was subjected to immunoprecipitation with 1 µg of PP2A-specific Ab, and HRP-conjugated streptavidin was used to detect immunocomplexes labeled with N-(biotinoyl)-N’-(iodoacetyl) ethylenediamine (BIAM) (Invitrogen) for development with an enhanced chemiluminescence kit. .. Transfection of siRNASmall interfering RNA (siRNA) against PP2A (a pool of 3 target-specific 19–25 nt siRNAs designed to knock down gene expression) or scrambled siRNA (scRNA) was transfected into pre-OCs that had previously been incubated with M-CSF and RANKL for 40 h using Lipofectamine 3000 as described [ ].

    Article Title: The Membrane Topography of Diphtheria Toxin T Domain Linked to the A Chain Reveals a Transient Transmembrane Hairpin and Potential Translocation Mechanisms
    Article Snippet: .. BODIPY-Streptavidin conjugate (BOD-SA) (discontinued except as a custom labeling product), Streptavidin (SA), N -(biotinoyl)- N ’-(iodoacetyl)-ethylenediamine (biotin-IA), were purchased from Molecular Probes (Eugene, OR). .. Endoproteinase Arg C was from Roche Diagnostics Corporation (Indianapolis, IN).

    Article Title: Comparative Proteomic Analysis of Cysteine Oxidation in Colorectal Cancer Patients
    Article Snippet: .. To assess patterns of oxidation, samples (∼50 mg) of frozen tumor tissue and tissue from non-tumor regions were homogenized in 500 μl of chilled labeling buffer [20 mM Tris-HCl (pH 7.5), 100 mM NaCl 1 mM EDTA, 0.1 mM AEBSF, leupeptin (2.0 μg/ml), and aprotinin (1.9 μg/ml)] containing 40 μM N-(biotinoyl)-N’-(iodoacetyl) ethylenediamine (biotinylated iodoacetamide, BIAM) (Molecular Probes, USA) and incubated for 4 h at 4°C. .. The labeling reaction was quenched by adding 4 mM dithiothreitol (DTT), after which the mixtures were centrifuged at 5, 000 rpm for 30 min at 4°C, and the supernatant were discarded.

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    Thermo Fisher ez link hpdp biotin
    Experimental workflow for the detection of palmitoylated proteins from primary human T cells. Pooled lysates from healthy human donors are initially divided into “Enriched” and “Control” samples. Disulfide bonds are reduced by tris(2-carboxyethyl)phosphine (TCEP), and free thiols are blocked by N-ethylmaleimide (NEM). Following cleavage of the palmitoyl thioester bond by hydroxylamine (HA) in the enriched sample, previously palmitoylated thiols are biotinylated using the sulfhydryl-reactive EZ-Link <t>HPDP-biotin.</t> Control samples omit HA cleavage, and remain unbiotinylated. Following enrichment via streptavidin-agarose beads and elution by beta-mercaptoethanol (2-ME), enriched and control samples are run in parallel lanes of an <t>SDS-PAGE</t> gel. These lanes are cut into equal-sized bands and subjected to tryptic digest in either heavy (H 2 18 O) or light (H 2 16 O) water, providing the isotopic label. After digestion, samples are mixed and measured by LC-MS/MS; evaluating the isotopic heavy/light intensity ratio gives rise to an enriched pool representing the population of palmitoylated proteins in the cell.
    Ez Link Hpdp Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link hpdp biotin/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    ez link hpdp biotin - by Bioz Stars, 2020-09
    99/100 stars
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    88
    Thermo Fisher n 6 biotinamido hexyl 3
    Experimental workflow for the detection of palmitoylated proteins from primary human T cells. Pooled lysates from healthy human donors are initially divided into “Enriched” and “Control” samples. Disulfide bonds are reduced by tris(2-carboxyethyl)phosphine (TCEP), and free thiols are blocked by N-ethylmaleimide (NEM). Following cleavage of the palmitoyl thioester bond by hydroxylamine (HA) in the enriched sample, previously palmitoylated thiols are biotinylated using the sulfhydryl-reactive EZ-Link <t>HPDP-biotin.</t> Control samples omit HA cleavage, and remain unbiotinylated. Following enrichment via streptavidin-agarose beads and elution by beta-mercaptoethanol (2-ME), enriched and control samples are run in parallel lanes of an <t>SDS-PAGE</t> gel. These lanes are cut into equal-sized bands and subjected to tryptic digest in either heavy (H 2 18 O) or light (H 2 16 O) water, providing the isotopic label. After digestion, samples are mixed and measured by LC-MS/MS; evaluating the isotopic heavy/light intensity ratio gives rise to an enriched pool representing the population of palmitoylated proteins in the cell.
    N 6 Biotinamido Hexyl 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 6 biotinamido hexyl 3/product/Thermo Fisher
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    n 6 biotinamido hexyl 3 - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    Experimental workflow for the detection of palmitoylated proteins from primary human T cells. Pooled lysates from healthy human donors are initially divided into “Enriched” and “Control” samples. Disulfide bonds are reduced by tris(2-carboxyethyl)phosphine (TCEP), and free thiols are blocked by N-ethylmaleimide (NEM). Following cleavage of the palmitoyl thioester bond by hydroxylamine (HA) in the enriched sample, previously palmitoylated thiols are biotinylated using the sulfhydryl-reactive EZ-Link HPDP-biotin. Control samples omit HA cleavage, and remain unbiotinylated. Following enrichment via streptavidin-agarose beads and elution by beta-mercaptoethanol (2-ME), enriched and control samples are run in parallel lanes of an SDS-PAGE gel. These lanes are cut into equal-sized bands and subjected to tryptic digest in either heavy (H 2 18 O) or light (H 2 16 O) water, providing the isotopic label. After digestion, samples are mixed and measured by LC-MS/MS; evaluating the isotopic heavy/light intensity ratio gives rise to an enriched pool representing the population of palmitoylated proteins in the cell.

    Journal: Scientific Reports

    Article Title: Quantitative analysis of the human T cell palmitome

    doi: 10.1038/srep11598

    Figure Lengend Snippet: Experimental workflow for the detection of palmitoylated proteins from primary human T cells. Pooled lysates from healthy human donors are initially divided into “Enriched” and “Control” samples. Disulfide bonds are reduced by tris(2-carboxyethyl)phosphine (TCEP), and free thiols are blocked by N-ethylmaleimide (NEM). Following cleavage of the palmitoyl thioester bond by hydroxylamine (HA) in the enriched sample, previously palmitoylated thiols are biotinylated using the sulfhydryl-reactive EZ-Link HPDP-biotin. Control samples omit HA cleavage, and remain unbiotinylated. Following enrichment via streptavidin-agarose beads and elution by beta-mercaptoethanol (2-ME), enriched and control samples are run in parallel lanes of an SDS-PAGE gel. These lanes are cut into equal-sized bands and subjected to tryptic digest in either heavy (H 2 18 O) or light (H 2 16 O) water, providing the isotopic label. After digestion, samples are mixed and measured by LC-MS/MS; evaluating the isotopic heavy/light intensity ratio gives rise to an enriched pool representing the population of palmitoylated proteins in the cell.

    Article Snippet: Following removal of NEM by three sequential CM precipitation steps, heavy SILAC samples were incubated for 1 hr at room temperature with rotational motion in +HA buffer (50 mM Tris (pH 7.4), 5 mM EDTA, 125 mM NaCl, 1% SDS, 574 mM hydroxylamine (HA) (Sigma-Aldrich), 820 μM EZ-Link HPDP-biotin (Thermo), 0.2% Triton X-100, 1 mM PMSF and PI-cocktail), while light SILAC samples were incubated in –HA buffer (same as +HA buffer but omitting HA); in a parallel enrichment, the heavy/light labels were also reversed.

    Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry