n 6 2 phenylisopropyl adenosine  (Millipore)


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    Name:
    N6 2 Phenylisopropyl adenosine
    Description:

    Catalog Number:
    p4532
    Price:
    None
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    Structured Review

    Millipore n 6 2 phenylisopropyl adenosine
    N6 2 Phenylisopropyl adenosine

    https://www.bioz.com/result/n 6 2 phenylisopropyl adenosine/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n 6 2 phenylisopropyl adenosine - by Bioz Stars, 2020-09
    93/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Mice with Deficiency of G Protein ?3 Are Lean and Have Seizures
    Article Snippet: .. Slides were equilibrated in 50 mM Tris-HCl-3 mM MgCl2 -0.2 mM EGTA-100 mM NaCl (pH 7.4) (assay buffer) at 25°C for 10 min and were then preincubated in assay buffer containing 2 mM GDP and adenosine deaminase (9.5 mU/ml) at 25°C for 15 min. Assays were conducted by incubating slides in assay buffer with 2 mM GDP, adenosine deaminase (9.5 mU/ml), and 0.04 nM [35 S]GTPγS (250 Ci/mmol; New England Nuclear Corp., Boston, Mass.) with (stimulated) or without (basal) agonist at 25°C for 2 h. Agonists included [ d -Ala2 ,N-Me-Phe4 ,Gly5 -ol]-enkephalin (DAMGO) (Drug Supply Program, National Institute on Drug Abuse), WIN 55,212-2, and ()-N6 -(2-phenylisopropyl)adenosine (PIA) (Sigma Chemical Co., St. Louis, Mo.) at a concentration (10 μM) that has been shown to produce maximal, antagonist-reversible stimulation ( , ). .. Slides were rinsed twice in 50 mM Tris-HCl, pH 7.4, at 4°C for 2 min each time and then in H2 O at 4°C for 30 s. Slides were dried overnight and exposed to Biomax MR film (Eastman Kodak Co., Rochester, N.Y.) for 24 h in the presence of 14 C-labeled microscales.

    Article Title: Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices
    Article Snippet: .. Mature adipocytes Overnight incubated mature adipocytes were washed in KRHG to remove media, suspended in KRHG at a concentration of 200 µl packed cells/mL and incubated 30 min with 10 nM (-)-N6-(2-phenylisopropyl)adenosine (PIA) (Sigma-Aldrich, St. Louis, MO, USA) and 0.5 U/mL adenosine deaminase (ADA) (Sigma-Aldrich, St. Louis, MO, USA). .. The cell suspension was subjected to centrifugation through diisonylphtalate and immediately frozen on dry ice until thawed by mixing with Ripa buffer (Sigma-Aldrich, St. Louis, MO, USA), phosphatase inhibitors (phosphostop, Roche) and protease inhibitors (complete mini, Roche) and stored at −20°C until analysis by western blot.

    Incubation:

    Article Title: Effects of the Human Immunodeficiency Virus-Protease Inhibitor, Ritonavir, on Basal and Catecholamine-Stimulated Lipolysis
    Article Snippet: .. In some experiments, before addition of Iso, 3T3-L1 adipocytes were incubated with 50 μ m PD 98059 (Sigma) or 1 μ m (-)-N6 -(2-phenylisopropyl)adenosine (PIA; Sigma) for times indicated in the figure legends. .. Whole-cell lysates were collected for DNA and cAMP analyses using the same lysis buffer as described above, except that whole-cell lysates collected for cAMP analyses were harvested in lysing buffer plus 0.25 m m IBMX.

    Article Title: Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices
    Article Snippet: .. Mature adipocytes Overnight incubated mature adipocytes were washed in KRHG to remove media, suspended in KRHG at a concentration of 200 µl packed cells/mL and incubated 30 min with 10 nM (-)-N6-(2-phenylisopropyl)adenosine (PIA) (Sigma-Aldrich, St. Louis, MO, USA) and 0.5 U/mL adenosine deaminase (ADA) (Sigma-Aldrich, St. Louis, MO, USA). .. The cell suspension was subjected to centrifugation through diisonylphtalate and immediately frozen on dry ice until thawed by mixing with Ripa buffer (Sigma-Aldrich, St. Louis, MO, USA), phosphatase inhibitors (phosphostop, Roche) and protease inhibitors (complete mini, Roche) and stored at −20°C until analysis by western blot.

    Binding Assay:

    Article Title: Comparative Study of Carborane- and Phenyl-Modified Adenosine Derivatives as Ligands for the A2A and A3 Adenosine Receptors Based on a Rigid in Silico Docking and Radioligand Replacement Assay
    Article Snippet: .. Non-specific binding was determined in the presence of R-PIA 100 μM (Sigma P4532, St. Louis, MO, USA). .. The reaction mixture (Vt: 200 μL/well) was incubated at 25 °C for 180 min, after was filtered and washed six times with 250 μL wash buffer (Tris-HCl 50 mM pH = 7.4), before measuring in a microplate beta scintillation counter (Microbeta Trilux, PerkinElmer, Madrid, Spain).

    other:

    Article Title: G?14 links a variety of Gi- and Gs-coupled receptors to the stimulation of phospholipase C
    Article Snippet: Carbachol, complement C5a, [ D -Ala2 , N -Me-Phe4 ,Gly5 -ol]enkephalin (DAGO), dopamine, [ D -Pen2,5 ]enkephalin (DPDPE), N -formylmethionylleucylphenylalanine (fMLP), 5-hydroxytryptamine (5-HT), human choriogonadotropin (hCG), 2-iodomelatonin, isoproterenol, nociceptin, (+)- N 6 -(2-phenylisopropyl)-adenosine (PIA), platelet activating factor (PAF), secretin, somatostatin, trans -(±)-3,4-dichloro-N-methyl-[2-(1-pyrrolidinyl)-cyclohexyl]-benzeneacetamide methanesulphonate (U-50,488H), and 5-bromo-N-(4,5-dihydro-1H-imidazol-2yl)-6-quinoxalinamine (UK14304) were purchased from Sigma (St. Louis, MO, U.S.A.) and Tocris Cookson Ltd. (Avonmouth, Bristol, U.K.).

    Mouse Assay:

    Article Title: Adenosine A3 receptors regulate heart rate, motor activity and body temperature
    Article Snippet: .. After 5 days of baseline registration some of the mice received the adenosine analogue R(−)N6-(2-phenylisopropyl) adenosine ( R-PIA, 50 μg/kg, Sigma, i.p.). .. Food intake, oxygen consumption, and locomotor activity were measured using a Comprehensive Lab Animal Monitoring System (Columbus Instruments, Columbus, OH).

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  • 99
    Millipore s aureus sa113
    Expression of IsdA and IsdB by S. aureus <t>SA113</t> after PGG treatment. S. aureus SA113 cells were cultured in TSBg (A), TSBg-PGG (B) medium, or TSBg-PGG medium containing 100 µM FeSO 4 (C) for 24 h. Proteins extracted from bacterial surface were analyzed by two-dimensional gel electrophoresis and by silver staining. The IsdA and IsdB spots (circles) were identified by MALDI-TOF spectrometry.
    S Aureus Sa113, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s aureus sa113/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    s aureus sa113 - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Millipore mcherry fusion proteins
    N-terminal region of PIASy locates to centromere independent of its remaining residues. A and B , localization of <t>mCherry-tagged</t> N-terminal peptides of PIASx and PIASy. Replicated chromosomes were obtained by incubating 500 sperm nuclei/μl in interphase
    Mcherry Fusion Proteins, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2 article reviews
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    mcherry fusion proteins - by Bioz Stars, 2020-09
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    Expression of IsdA and IsdB by S. aureus SA113 after PGG treatment. S. aureus SA113 cells were cultured in TSBg (A), TSBg-PGG (B) medium, or TSBg-PGG medium containing 100 µM FeSO 4 (C) for 24 h. Proteins extracted from bacterial surface were analyzed by two-dimensional gel electrophoresis and by silver staining. The IsdA and IsdB spots (circles) were identified by MALDI-TOF spectrometry.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Expression of IsdA and IsdB by S. aureus SA113 after PGG treatment. S. aureus SA113 cells were cultured in TSBg (A), TSBg-PGG (B) medium, or TSBg-PGG medium containing 100 µM FeSO 4 (C) for 24 h. Proteins extracted from bacterial surface were analyzed by two-dimensional gel electrophoresis and by silver staining. The IsdA and IsdB spots (circles) were identified by MALDI-TOF spectrometry.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Expressing, Cell Culture, Two-Dimensional Gel Electrophoresis, Electrophoresis, Silver Staining

    Effect of iron on biofilm formation. S. aureus SA113 was cultured in TSBg, TSBg-PGG and TSBg-PGG medium containing FeSO 4 in wells in a 96-well microtiter plate. After incubation at 37°C for 24 h, biofilm formation in the well was measured by safranin-staining method (A). The amount of biofilm formed by the control cells that was treated with DMSO was set to 100%. Biofilm formation is presented as percentage of that in the control cells. Experiments were performed three times, and each sample in the experiment was prepared in six wells. Error bars represent standard error. (B) Cells were cultured in TSBg, TSBg-PGG, and TSBg-PGG containing 100 µM FeSO 4 . After incubation for 24 h, the biofilm that had formed on the coverslips was stained using a LIVE/DEAD Bac Light Bacterial Viability kit (Invitrogen). Cells not treated with PGG were used as the control. The biofilm structure was examined under a confocal laser-scanning microscope. (a–c) Images reconstructed from a series of Z sections. (d–f) Images reconstructed from average intensity projection through confocal image stacks of series of X-Z (bottom) and Y-Z (right) sections. The number at the bottom represents the thickness (µm) of the biofilm. The bar represents 25 µm.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Effect of iron on biofilm formation. S. aureus SA113 was cultured in TSBg, TSBg-PGG and TSBg-PGG medium containing FeSO 4 in wells in a 96-well microtiter plate. After incubation at 37°C for 24 h, biofilm formation in the well was measured by safranin-staining method (A). The amount of biofilm formed by the control cells that was treated with DMSO was set to 100%. Biofilm formation is presented as percentage of that in the control cells. Experiments were performed three times, and each sample in the experiment was prepared in six wells. Error bars represent standard error. (B) Cells were cultured in TSBg, TSBg-PGG, and TSBg-PGG containing 100 µM FeSO 4 . After incubation for 24 h, the biofilm that had formed on the coverslips was stained using a LIVE/DEAD Bac Light Bacterial Viability kit (Invitrogen). Cells not treated with PGG were used as the control. The biofilm structure was examined under a confocal laser-scanning microscope. (a–c) Images reconstructed from a series of Z sections. (d–f) Images reconstructed from average intensity projection through confocal image stacks of series of X-Z (bottom) and Y-Z (right) sections. The number at the bottom represents the thickness (µm) of the biofilm. The bar represents 25 µm.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Cell Culture, Incubation, Staining, BAC Assay, Laser-Scanning Microscopy

    Iron restores biofilm formation in iron-restricted medium. S. aureus SA113 was cultured in BM medium that contained PGG (A, B, C) or 2-DP (D, E, F) in wells in a 96-well microtiter plate. Following incubation at 37°C for 24 h, the cell density was determined at A578 (A, D). The amount of biofilm formation in the well was determined at A490 after safranin staining (B, C, D, E). After FeSO4 was added to BM medium that contained 12.5 µM PGG (C) or 1 mM 2-DP (F) and incubated at 37°C for 24 h, the biofilm that was formed in the well was washed and stained by safranin. The cell density and biofilm formation by the cells that were treated with either DMSO or distilled water were used as controls and set to 100%. Experiments were performed three times, and each sample in each experiment was prepared in six wells. The error bar represents the standard error.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Iron restores biofilm formation in iron-restricted medium. S. aureus SA113 was cultured in BM medium that contained PGG (A, B, C) or 2-DP (D, E, F) in wells in a 96-well microtiter plate. Following incubation at 37°C for 24 h, the cell density was determined at A578 (A, D). The amount of biofilm formation in the well was determined at A490 after safranin staining (B, C, D, E). After FeSO4 was added to BM medium that contained 12.5 µM PGG (C) or 1 mM 2-DP (F) and incubated at 37°C for 24 h, the biofilm that was formed in the well was washed and stained by safranin. The cell density and biofilm formation by the cells that were treated with either DMSO or distilled water were used as controls and set to 100%. Experiments were performed three times, and each sample in each experiment was prepared in six wells. The error bar represents the standard error.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Cell Culture, Incubation, Staining

    Effect of iron on adherence of S. aureus to solid surfaces and PIA synthesis. S. aureus SA113 (A) and clinical strains, i.e. , SA130, SA148, SA229, SA435 (C) were inoculated in TSBg that contained 0 µM and 25 µM of PGG (TSBg-PGG) or supplemented with FeSO 4 in 9-cm petri dishes. After 24 h incubation, biofilm that formed on the plate was photographed. The plates were tilted and photographed to show the attachment of cells to the petri plate and the clumping of cells in the medium in the lower half of the plate. (B) PIA was extracted from S. aureus SA113 that had been cultured for 24 h in TSBg or TSBg-PGG medium containing 0, 50 and 100 µM FeSO 4 . PIA was detected using WGA-biotin. After incubation with HRP-streptavidin, the spots were visualized by chemiluminescence detection.

    Journal: PLoS ONE

    Article Title: Involvement of Iron in Biofilm Formation by Staphylococcus aureus

    doi: 10.1371/journal.pone.0034388

    Figure Lengend Snippet: Effect of iron on adherence of S. aureus to solid surfaces and PIA synthesis. S. aureus SA113 (A) and clinical strains, i.e. , SA130, SA148, SA229, SA435 (C) were inoculated in TSBg that contained 0 µM and 25 µM of PGG (TSBg-PGG) or supplemented with FeSO 4 in 9-cm petri dishes. After 24 h incubation, biofilm that formed on the plate was photographed. The plates were tilted and photographed to show the attachment of cells to the petri plate and the clumping of cells in the medium in the lower half of the plate. (B) PIA was extracted from S. aureus SA113 that had been cultured for 24 h in TSBg or TSBg-PGG medium containing 0, 50 and 100 µM FeSO 4 . PIA was detected using WGA-biotin. After incubation with HRP-streptavidin, the spots were visualized by chemiluminescence detection.

    Article Snippet: Detection of PIA The PIA extracted from S. aureus SA113 was blotted onto PVDF membrane (Millipore, Billerica, MA) using a 96-well dot-blot apparatus according to a method described elsewhere .

    Techniques: Incubation, Cell Culture, Whole Genome Amplification

    N-terminal region of PIASy locates to centromere independent of its remaining residues. A and B , localization of mCherry-tagged N-terminal peptides of PIASx and PIASy. Replicated chromosomes were obtained by incubating 500 sperm nuclei/μl in interphase

    Journal: The Journal of Biological Chemistry

    Article Title: Rod/Zw10 Complex Is Required for PIASy-dependent Centromeric SUMOylation *

    doi: 10.1074/jbc.M110.153817

    Figure Lengend Snippet: N-terminal region of PIASy locates to centromere independent of its remaining residues. A and B , localization of mCherry-tagged N-terminal peptides of PIASx and PIASy. Replicated chromosomes were obtained by incubating 500 sperm nuclei/μl in interphase

    Article Snippet: All N-terminal fragments of PIAS family and mCherry fusion proteins were expressed in BL21 (DE3) or Rossetta2 (DE3) (EMD Biosciences) and purified with Talone metal affinity resin (Clonthech) followed by ion-exchange chromatography.

    Techniques: