Journal: Bioengineered

Article Title: Interleukin-17A attenuates photoreceptor cell apoptosis in streptozotocin-induced diabetic mouse model

doi: 10.1080/21655979.2022.2084241

Figure Lengend Snippet: The lack of IL-17A blocks high glucose-induced mature NT-3 production . (a, b) Representative immunoblots and densitometric quantitation of proNT-3 and mature NT-3 protein amounts in MMC isolated from IL-17A −/− and WT mice, under NG or HG conditions for 18 hours. (c) Representative immunoblots and densitometric quantitation of proNT-3 and mature NT-3 protein amounts in MMC pretreated with various amounts of recombinant IL-17A (0, 10, 50, 100 and 200 ng/mL) for 18 hours. Data are mean ± SEM (n = 3 independent samples t test, **P < 0.01, ***P < 0.001 compared to 0 ng/mL group). Note: MMC, Müller cell; HG, high glucose; NG, normal glucose; WT, wild type.

Article Snippet: This was followed by overnight incubation at 4°C with anti-IL-17A (1/200, Abcam, Cambridge, MA, USA), anti-glutamine synthetase (GS; 1/1000, Abcam), anti-GFAP (1/10 000, Abcam), anti-Bcl-2 (1/1000, Abcam), anti-Bax (1/2000, Abcam), anti-active caspase-3 (1/500, Abcam), anti-NT-3 (1/500, Alomone Labs, Jerusalem, Israel), anti-pro-NT3 (1/500, Alomone Labs), anti-TrkC (1/2000, Abcam), anti-P75 NTR (1/2000, Abcam), and anti-β-actin (1/5000; Cell Signaling Technology, Beverly, MA, USA).

Techniques: Western Blot, Quantitation Assay, Isolation, Recombinant