Structured Review

Fisher Scientific hepes buffer
Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of <t>HEPES</t> concentration (e) Optimization of <t>NaCl</t> concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.
Hepes Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities"

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities

Journal: Heliyon

doi: 10.1016/j.heliyon.2018.e01023

Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.
Figure Legend Snippet: Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

Techniques Used: Titration, Concentration Assay, Incubation

2) Product Images from "Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities"

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities

Journal: Heliyon

doi: 10.1016/j.heliyon.2018.e01023

Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.
Figure Legend Snippet: Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

Techniques Used: Titration, Concentration Assay, Incubation

3) Product Images from "Impact of Carrier Fluid Composition on Recovery of Nanoparticles and Proteins in Flow Field Flow Fractionation"

Article Title: Impact of Carrier Fluid Composition on Recovery of Nanoparticles and Proteins in Flow Field Flow Fractionation

Journal: Journal of chromatography. A

doi: 10.1016/j.chroma.2012.09.050

(a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding NaCl to 10 mM HEPES (pH 7.3).
Figure Legend Snippet: (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding NaCl to 10 mM HEPES (pH 7.3).

Techniques Used:

4) Product Images from "Cations induce shape remodeling of negatively charged phospholipid membranes"

Article Title: Cations induce shape remodeling of negatively charged phospholipid membranes

Journal: Physical chemistry chemical physics : PCCP

doi: 10.1039/c7cp00718c

binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .
Figure Legend Snippet: binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .

Techniques Used: Binding Assay, Titration

5) Product Images from "Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells"

Article Title: Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2014.00270

The Panx1 channels do not contribute to the Ca 2+ signal induced by caffeine but form functional channels at the plasma membrane. (A,B) Indo-1 loaded chromaffin cells were maintained in a Ca 2+ -free Kreb’s Hepes solution (in mM: 140 NaCl, 5.9 KCl, 1.2 MgCl 2 , 2 EGTA, 10 Hepes-NaOH, 10 glucose) and stimulated with a 10 s pulse of 50 mM caffeine. The cytosolic [Ca 2+ ] was monitored by microfluorometry. Data show means ± SEM of basal cytosolic [Ca 2+ ] (A) or the maximum amplitude of the Ca 2+ signals induced by caffeine (B) in control cells ( n = 22), or cells treated with probenecid ( n = 22) or Cbx ( n = 21). ns = non-significant, * p
Figure Legend Snippet: The Panx1 channels do not contribute to the Ca 2+ signal induced by caffeine but form functional channels at the plasma membrane. (A,B) Indo-1 loaded chromaffin cells were maintained in a Ca 2+ -free Kreb’s Hepes solution (in mM: 140 NaCl, 5.9 KCl, 1.2 MgCl 2 , 2 EGTA, 10 Hepes-NaOH, 10 glucose) and stimulated with a 10 s pulse of 50 mM caffeine. The cytosolic [Ca 2+ ] was monitored by microfluorometry. Data show means ± SEM of basal cytosolic [Ca 2+ ] (A) or the maximum amplitude of the Ca 2+ signals induced by caffeine (B) in control cells ( n = 22), or cells treated with probenecid ( n = 22) or Cbx ( n = 21). ns = non-significant, * p

Techniques Used: Functional Assay

6) Product Images from "Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments"

Article Title: Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments

Journal: Journal of materials chemistry

doi: 10.1039/C2JM35019J

Cage-like assemblies of Hub-His 6 and Hub-NoTag. pH-dependent assembly and disassembly of (a) Hub-His 6 and (b) Hub-NoTag, monitored by light scattering at 320 nm. Each protein preparation (0.1 mg/mL,100 µL) was assembled by the addition of 10 µL of 1.5 M HEPES buffer (pH 6.5) at the respective times (as indicated by the white arrows); disassembly was triggered with the addition of 11µL of 1.0 M Tris buffer (pH 9.0) at the respective times (black arrows). Stained TEM images of (c) Hub-His 6 and (d) Hub-NoTag were obtained. Scale bar represents 200 nm.
Figure Legend Snippet: Cage-like assemblies of Hub-His 6 and Hub-NoTag. pH-dependent assembly and disassembly of (a) Hub-His 6 and (b) Hub-NoTag, monitored by light scattering at 320 nm. Each protein preparation (0.1 mg/mL,100 µL) was assembled by the addition of 10 µL of 1.5 M HEPES buffer (pH 6.5) at the respective times (as indicated by the white arrows); disassembly was triggered with the addition of 11µL of 1.0 M Tris buffer (pH 9.0) at the respective times (black arrows). Stained TEM images of (c) Hub-His 6 and (d) Hub-NoTag were obtained. Scale bar represents 200 nm.

Techniques Used: Staining, Transmission Electron Microscopy

7) Product Images from "Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles"

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles

Journal: Pharmaceutical research

doi: 10.1007/s11095-012-0904-4

VIP binding in 100 mM NaCl, 100 mM HEPES at a carrier concentration of 2.4 mg/ml for PGC and 2.0 mg/ml for SSM ( a ) PGC-VIP, ( b ) SSM-VIP. The experiment was done in quadruplicate; free VIP was separated from bound by ultrafiltration, VIP quantitation was
Figure Legend Snippet: VIP binding in 100 mM NaCl, 100 mM HEPES at a carrier concentration of 2.4 mg/ml for PGC and 2.0 mg/ml for SSM ( a ) PGC-VIP, ( b ) SSM-VIP. The experiment was done in quadruplicate; free VIP was separated from bound by ultrafiltration, VIP quantitation was

Techniques Used: Binding Assay, Concentration Assay, Pyrolysis Gas Chromatography, Quantitation Assay

8) Product Images from "Yeast Frataxin Is Stabilized by Low Salt Concentrations: Cold Denaturation Disentangles Ionic Strength Effects from Specific Interactions"

Article Title: Yeast Frataxin Is Stabilized by Low Salt Concentrations: Cold Denaturation Disentangles Ionic Strength Effects from Specific Interactions

Journal: PLoS ONE

doi: 10.1371/journal.pone.0095801

Thermal denaturation curves of Yfh1 in the presence of salts. A) Thermal denaturation curves of 10 µM Yfh1 in a 10 mM HEPES buffer at pH 7.5, measured by monitoring the CD intensity at 220 nm as a function of temperature in the temperature range 0°C to 80°C in the presence of varying concentrations of NaCl, KCl, MgCl 2 and CaCl 2 . Denaturation curves in the presence of NaCl/KCl shown between the two extremes (“no salt” and 100 mM) have the following concentrations: 5/10/25/50 mM. Denaturation curves in the presence of MgCl 2 shown between the two extremes (“no salt” and 1 mM) have the following concentrations: 0.05/0.1/0.2/0.3/0.4 mM. Denaturation curves in the presence of CaCl 2 shown between the two extremes (“no salt” and 0.4 mM) have the following concentrations 0.05/0.08/0.1/0.2/0.3 mM. B) corresponding curves in the presence of varying concentrations of NaF, NaCl, NaH 2 PO 4 and Na 2 SO 4 . Curves shown between the two extremes (“no salt” and 100 mM) have the following millimolar concentrations: 5/10/25/50.
Figure Legend Snippet: Thermal denaturation curves of Yfh1 in the presence of salts. A) Thermal denaturation curves of 10 µM Yfh1 in a 10 mM HEPES buffer at pH 7.5, measured by monitoring the CD intensity at 220 nm as a function of temperature in the temperature range 0°C to 80°C in the presence of varying concentrations of NaCl, KCl, MgCl 2 and CaCl 2 . Denaturation curves in the presence of NaCl/KCl shown between the two extremes (“no salt” and 100 mM) have the following concentrations: 5/10/25/50 mM. Denaturation curves in the presence of MgCl 2 shown between the two extremes (“no salt” and 1 mM) have the following concentrations: 0.05/0.1/0.2/0.3/0.4 mM. Denaturation curves in the presence of CaCl 2 shown between the two extremes (“no salt” and 0.4 mM) have the following concentrations 0.05/0.08/0.1/0.2/0.3 mM. B) corresponding curves in the presence of varying concentrations of NaF, NaCl, NaH 2 PO 4 and Na 2 SO 4 . Curves shown between the two extremes (“no salt” and 100 mM) have the following millimolar concentrations: 5/10/25/50.

Techniques Used:

Thermal denaturation of Yfh1 at comparable anion and cation concentrations. Thermal denaturation curves of 10 µM Yfh1 in a 10 mM HEPES buffer at pH 7.5, measured by monitoring the CD intensity at 220 nm as a function of temperature in the temperature range 0°C to 80°C in the presence of A) 2 mM NaF, NaCl, NaH 2 PO 4 and Na 2 SO 4 B) 2 mM NaCl, KCl, MgCl 2 and CaCl 2 , C) 25 and 50 µM FeSO 4 .
Figure Legend Snippet: Thermal denaturation of Yfh1 at comparable anion and cation concentrations. Thermal denaturation curves of 10 µM Yfh1 in a 10 mM HEPES buffer at pH 7.5, measured by monitoring the CD intensity at 220 nm as a function of temperature in the temperature range 0°C to 80°C in the presence of A) 2 mM NaF, NaCl, NaH 2 PO 4 and Na 2 SO 4 B) 2 mM NaCl, KCl, MgCl 2 and CaCl 2 , C) 25 and 50 µM FeSO 4 .

Techniques Used:

Stability curves and thermodynamic parameters. A) Comparison of the stability curves for Yfh1 in HEPES alone (no salt) and in the presence of three representative salts of divalent cations at concentrations that yield the maximum value of ΔG. The dotted lines show average values of T S . B) Comparison of the stability curves for Yfh1 in HEPES alone (no salt) and in the presence of five representative salts of monovalent cations at concentrations that yield the maximum value of ΔG. The dotted lines show average values of T S . C) Dependence of Δ G max for divalent cations as a function of concentration. D) Δ G max for all anions as a function of concentration.
Figure Legend Snippet: Stability curves and thermodynamic parameters. A) Comparison of the stability curves for Yfh1 in HEPES alone (no salt) and in the presence of three representative salts of divalent cations at concentrations that yield the maximum value of ΔG. The dotted lines show average values of T S . B) Comparison of the stability curves for Yfh1 in HEPES alone (no salt) and in the presence of five representative salts of monovalent cations at concentrations that yield the maximum value of ΔG. The dotted lines show average values of T S . C) Dependence of Δ G max for divalent cations as a function of concentration. D) Δ G max for all anions as a function of concentration.

Techniques Used: Concentration Assay

Comparison of NMR spectra of Yfh1. A) 15 N-HSQC NMR spectra of 0.25 mM Yfh1 in HEPES (blue cross peaks). B) (green cross peaks) in the presence of 200∶1 NaCl. 15 N-HSQC NMR spectra of 0.25 mM Yfh1 in HEPES and C) (red cross peaks) in the presence of 1∶1 CaCl 2 . The fourth panel (D, lower right) shows the superposition of the region of the three spectra in which it is possible to observe the largest changes. The outstanding chemical shift changes involving L102 and E103 are highlighted by arrows Residue numbers are those of PDB ID:2FQL.
Figure Legend Snippet: Comparison of NMR spectra of Yfh1. A) 15 N-HSQC NMR spectra of 0.25 mM Yfh1 in HEPES (blue cross peaks). B) (green cross peaks) in the presence of 200∶1 NaCl. 15 N-HSQC NMR spectra of 0.25 mM Yfh1 in HEPES and C) (red cross peaks) in the presence of 1∶1 CaCl 2 . The fourth panel (D, lower right) shows the superposition of the region of the three spectra in which it is possible to observe the largest changes. The outstanding chemical shift changes involving L102 and E103 are highlighted by arrows Residue numbers are those of PDB ID:2FQL.

Techniques Used: Nuclear Magnetic Resonance

Related Articles

Clone Assay:

Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
Article Snippet: Co-immunoprecipitation Esrrb-coding sequence (PiggyBac-Esrrb-ires-Neo, a kind gift from Austin Smith, CSCR, Cambridge, UK) was cloned to result in PiggyBac-CAG-Avi-Esrrb-3xFlag-ires-Neo construct. .. Extracts were spun down and the pellet resuspended in Buffer C (20 mM Hepes pH 7.6, 25% Glycerol, 420 mM NaCl, 1.5 mM MgCl2 and 0.2 mM EDTA), passed through a 19-G needle and dialysed to Bufffer D (20 mM Hepes pH 7.6, 20% Glycerol, 100 mM KCl, 1.5 mM MgCl2 and 0.2 mM EDTA) using dialysis cassettes (Fisher Scientific).

Synthesized:

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities
Article Snippet: Peptide substrate (GGGFKEFAAGRKSLTLNLITEGGG) was synthesized and undergone quality control analysis using Mass Spectrometry and HPLC by GenScript (NJ, USA). .. HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

Article Title: Mechanistic investigations of matrix metalloproteinase-8 inhibition by metal abstraction peptide
Article Snippet: Two-hundred proof ethanol was obtained from Decon laboratories. (4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid) (HEPES), sodium chloride, potassium chloride, potassium phosphate monobasic, Tris base, hydrochloric acid (HCl), chloroform, acetone, acetic acid, nickel sulfate hexahydrate, and zinc sulfate were obtained from Fisher Scientific. .. Peptide SWLAYPGAVSYRG NCC (tether-MAP) was custom synthesized by GenScript ( > 95% purity), provided in small aliquots and stored at −20 °C to reduce freeze/thaw cycles of the peptide.

Pyrolysis Gas Chromatography:

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: .. VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate. .. The mixtures were subjected to ultrafiltration using 100 kDa MWCO cellulose membrane centrifuge filter (Amicon Ultra Ultracel 100 k, Millipore, Billerica MA) by centrifuging at 18,000×g for 12 min.

Construct:

Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
Article Snippet: TS EGFP cells were transfected with the construct along with the empty vector control using Lipofectamine 2000 (Invitrogen), selected with G418 and expanded in 10 15-cm dishes. .. Extracts were spun down and the pellet resuspended in Buffer C (20 mM Hepes pH 7.6, 25% Glycerol, 420 mM NaCl, 1.5 mM MgCl2 and 0.2 mM EDTA), passed through a 19-G needle and dialysed to Bufffer D (20 mM Hepes pH 7.6, 20% Glycerol, 100 mM KCl, 1.5 mM MgCl2 and 0.2 mM EDTA) using dialysis cassettes (Fisher Scientific).

Ubiquitin Assay:

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: .. Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

Incubation:

Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
Article Snippet: Extracts were spun down and the pellet resuspended in Buffer C (20 mM Hepes pH 7.6, 25% Glycerol, 420 mM NaCl, 1.5 mM MgCl2 and 0.2 mM EDTA), passed through a 19-G needle and dialysed to Bufffer D (20 mM Hepes pH 7.6, 20% Glycerol, 100 mM KCl, 1.5 mM MgCl2 and 0.2 mM EDTA) using dialysis cassettes (Fisher Scientific). .. Anti-FLAG M2 agarose beads (120 μl; Sigma) equilibrated in buffer D were added to 1.5 ml of nuclear extract in No Stick microcentrifuge tubes (Alpha Laboratories) and incubated for 3 h at 4 °C in the presence of Benzonase (Novagen).

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

Mass Spectrometry:

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities
Article Snippet: Peptide substrate (GGGFKEFAAGRKSLTLNLITEGGG) was synthesized and undergone quality control analysis using Mass Spectrometry and HPLC by GenScript (NJ, USA). .. HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

High Performance Liquid Chromatography:

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities
Article Snippet: Peptide substrate (GGGFKEFAAGRKSLTLNLITEGGG) was synthesized and undergone quality control analysis using Mass Spectrometry and HPLC by GenScript (NJ, USA). .. HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate. .. VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate.

Slice Preparation:

Article Title: Immunofluorescence staining of live lymph node tissue slices
Article Snippet: Paragraph title: 2.2. Slice preparation ... Complete media consisted of RPMI (Lonza RPMI 1640 without L-glutamine, #12-167F) supplemented with 10% FBS, 1% L-glutamine, and 1% Pen/Strep, 50 μM beta-mercaptoethanol, 1 mM pyruvate, 1% non-essential amino acids, and 20 mM HEPES (Fisher Scientific).

Flow Cytometry:

Article Title: Yeast Frataxin Is Stabilized by Low Salt Concentrations: Cold Denaturation Disentangles Ionic Strength Effects from Specific Interactions
Article Snippet: All samples used Yfh1 at 10 µM in a 10 mM HEPES buffer at pH 7.5 and varying concentrations of NaCl, KCl (Fisher Scientific), MgCl2 , CaCl2 , FeSO4 , NaF (Sigma-Aldrich), NaH2 PO4 (Acros Organics) and Na2 SO4 (Merck). .. In the case of the ferrous salt rigorous anaerobic conditions were achieved by preparing the samples in a glove box, sealing the cuvette and performing the measurements under N2 (g) flow.

Immunoprecipitation:

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: .. Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

Protease Inhibitor:

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: .. Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

other:

Article Title: Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells
Article Snippet: Catecholamine secretion from the adrenal gland Fresh bovine adrenal glands were perfused with a Kreb’s-Hepes solution (in mM: 140 NaCl, 5.9 KCl, 1.2 MgCl2 , 2 CaCl2 , 10 Hepes-NaOH, 10 glucose) by means of a peristaltic pump (Variable-speed pump 2, Fisher Scientific, Waltham, USA) at a rate of 4 mL/min.

Article Title: Dynamic Effects of Hg2+-induced Changes in Cell Volume
Article Snippet: N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid (HEPES) and calcium chloride were obtained from Fisher Chemical (Fairlawn, NJ).

Article Title: Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments
Article Snippet: Imidazole, tris base, dithiothreitol (DTT), Isopropyl β-D-1-thiogalactopyranoside (IPTG), HEPES were purchased from Fisher Scientific. (CH3 )3 PAuCl, Thrombin CleanCleave™ Kit and uranyl acetate were purchased from Sigma Aldrich.

Article Title: The hidden structure of human enamel
Article Snippet: Polishing The samples were polished using 300 nm and then 50 nm alumina suspensions (Buehler, Lake Bluff, IL), saturated with HEPES buffer, pH 8.0 (Fisher Scientific, Waltham, MA) to prevent apatite dissolution.

Sequencing:

Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
Article Snippet: Co-immunoprecipitation Esrrb-coding sequence (PiggyBac-Esrrb-ires-Neo, a kind gift from Austin Smith, CSCR, Cambridge, UK) was cloned to result in PiggyBac-CAG-Avi-Esrrb-3xFlag-ires-Neo construct. .. Extracts were spun down and the pellet resuspended in Buffer C (20 mM Hepes pH 7.6, 25% Glycerol, 420 mM NaCl, 1.5 mM MgCl2 and 0.2 mM EDTA), passed through a 19-G needle and dialysed to Bufffer D (20 mM Hepes pH 7.6, 20% Glycerol, 100 mM KCl, 1.5 mM MgCl2 and 0.2 mM EDTA) using dialysis cassettes (Fisher Scientific).

Sonication:

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The remaining pellet was then resuspended in lysis buffer 1 and then sonicated and spun down.

Binding Assay:

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: Paragraph title: Binding Studies and Determination of the Dissociation Constant (Kd) ... VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate.

Amplified Luminescent Proximity Homogenous Assay:

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities
Article Snippet: AlphaScreen® Detection Kit containing StrepTactin® donor beads (5 mg/ml), 10x buffer and nickel chelate acceptor beads (5 mg/ml) and white 384-well Opti-plate were purchased from Perkin Elmer (Santa Clara, CA, USA). .. HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

Isolation:

Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities
Article Snippet: HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium). .. Panduratin A, a natural compound was isolated as previously described .

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: .. Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

Transfection:

Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
Article Snippet: TS EGFP cells were transfected with the construct along with the empty vector control using Lipofectamine 2000 (Invitrogen), selected with G418 and expanded in 10 15-cm dishes. .. Extracts were spun down and the pellet resuspended in Buffer C (20 mM Hepes pH 7.6, 25% Glycerol, 420 mM NaCl, 1.5 mM MgCl2 and 0.2 mM EDTA), passed through a 19-G needle and dialysed to Bufffer D (20 mM Hepes pH 7.6, 20% Glycerol, 100 mM KCl, 1.5 mM MgCl2 and 0.2 mM EDTA) using dialysis cassettes (Fisher Scientific).

Plasmid Preparation:

Article Title: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells
Article Snippet: TS EGFP cells were transfected with the construct along with the empty vector control using Lipofectamine 2000 (Invitrogen), selected with G418 and expanded in 10 15-cm dishes. .. Extracts were spun down and the pellet resuspended in Buffer C (20 mM Hepes pH 7.6, 25% Glycerol, 420 mM NaCl, 1.5 mM MgCl2 and 0.2 mM EDTA), passed through a 19-G needle and dialysed to Bufffer D (20 mM Hepes pH 7.6, 20% Glycerol, 100 mM KCl, 1.5 mM MgCl2 and 0.2 mM EDTA) using dialysis cassettes (Fisher Scientific).

In Vitro:

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: .. Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

Produced:

Article Title: Impact of Carrier Fluid Composition on Recovery of Nanoparticles and Proteins in Flow Field Flow Fractionation
Article Snippet: Based on the manufacturer, these polystyrene particles were produced by emulsion polymerization using an anionic surfactant and had surface sulfate groups which arose from the polymerization initiator. .. All chemicals used in this study, such as hydrogen chloride (HCl), sodium hydroxide (NaOH), sodium dihydrogen phosphate (NaH2 PO4 ), disodium hydrogen phosphate (Na2 HPO4 ), the chloride salts of sodium, potassium, cesium, and magnesium (NaCl, KCl, CsCl, MgCl2 ), and the 1 M HEPES stock solution (pH 7.3), were acquired from Fisher Scientific (Pittsburgh, PA, USA).

Concentration Assay:

Article Title: Cations induce shape remodeling of negatively charged phospholipid membranes
Article Snippet: The concentration of lipid stock solutions was periodically verified by phosphate assay. .. CaCl2 , MgCl2 , NaCl, Casein, Tris, HEPES, DTT and EDTA were obtained from Fisher Scientific (Rochester, NY).

Article Title: Yeast Frataxin Is Stabilized by Low Salt Concentrations: Cold Denaturation Disentangles Ionic Strength Effects from Specific Interactions
Article Snippet: Samples were prepared using a Yfh1 concentration of 10 µM in 10 mM HEPES buffer at pH 7.5 and with a variety of salts: NaCl (Sigma-Adrich), KCl (Aldrich), MgCl2 (Aldrich), CaCl2 (J.T.Baker), NaF (May & Baker), NaH2 PO4 (Carlo Erba), Na2 SO4 (Sigma-Aldrich) and NaI (Carlo Erba). .. All samples used Yfh1 at 10 µM in a 10 mM HEPES buffer at pH 7.5 and varying concentrations of NaCl, KCl (Fisher Scientific), MgCl2 , CaCl2 , FeSO4 , NaF (Sigma-Aldrich), NaH2 PO4 (Acros Organics) and Na2 SO4 (Merck).

Article Title: Impact of Carrier Fluid Composition on Recovery of Nanoparticles and Proteins in Flow Field Flow Fractionation
Article Snippet: All chemicals used in this study, such as hydrogen chloride (HCl), sodium hydroxide (NaOH), sodium dihydrogen phosphate (NaH2 PO4 ), disodium hydrogen phosphate (Na2 HPO4 ), the chloride salts of sodium, potassium, cesium, and magnesium (NaCl, KCl, CsCl, MgCl2 ), and the 1 M HEPES stock solution (pH 7.3), were acquired from Fisher Scientific (Pittsburgh, PA, USA). .. The phosphate buffers were prepared from mixing the mono- and dibasic- phosphates at ratios calculated with the buffer design tool available on , to achieve the desired pH and phosphate concentration.

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: .. VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate. .. The mixtures were subjected to ultrafiltration using 100 kDa MWCO cellulose membrane centrifuge filter (Amicon Ultra Ultracel 100 k, Millipore, Billerica MA) by centrifuging at 18,000×g for 12 min.

Lysis:

Article Title: Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway
Article Snippet: .. Immunoprecipitation and in vitro Ubiquitylation Assay of Pulled-down Proteins Isolated 3D7 parasites were resuspended in 20 mM HEPES pH7.9, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF (Fisher Scientific), 0.65% Igepal v/v and cocktail protease inhibitor (Roche) (lysis buffer 1). .. The pellet was then subsequently resuspended in 20 mM HEPES pH 7.9, 0.1 M NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.5 mM MgCl2 , 1 mM DTT, 1 mM AEBSF and cocktail protease inhibitor (Roche) (lysis buffer 2), and then incubated at 4°C with vigorous shaking for 20 minutes.

Hood:

Article Title: Immunofluorescence staining of live lymph node tissue slices
Article Snippet: Complete media consisted of RPMI (Lonza RPMI 1640 without L-glutamine, #12-167F) supplemented with 10% FBS, 1% L-glutamine, and 1% Pen/Strep, 50 μM beta-mercaptoethanol, 1 mM pyruvate, 1% non-essential amino acids, and 20 mM HEPES (Fisher Scientific). .. For sterile slicing, the procedure differed in the following ways: The agarose was sterilized by autoclaving prior to embedding tissue, 1% Pen-Strep was added to PBS buffer during slicing, the vibratome was placed in a biosafety cabinet to prevent contamination, and the vibratome frequency was reduced to 10 Hz.

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  • 99
    Fisher Scientific hepes buffer
    Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of <t>HEPES</t> concentration (e) Optimization of <t>NaCl</t> concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.
    Hepes Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer/product/Fisher Scientific
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    hepes buffer - by Bioz Stars, 2020-03
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    77
    Fisher Scientific hepes stock solution
    (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding <t>NaCl</t> to 10 mM <t>HEPES</t> (pH 7.3).
    Hepes Stock Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes stock solution/product/Fisher Scientific
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes stock solution - by Bioz Stars, 2020-03
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    99
    Fisher Scientific hepes
    binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm <t>nacl</t> and 7 mm <t>hepes</t> at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .
    Hepes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Fisher Scientific
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Journal: Heliyon

    Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities

    doi: 10.1016/j.heliyon.2018.e01023

    Figure Lengend Snippet: Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Article Snippet: HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

    Techniques: Titration, Concentration Assay, Incubation

    Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Journal: Heliyon

    Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities

    doi: 10.1016/j.heliyon.2018.e01023

    Figure Lengend Snippet: Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Article Snippet: HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

    Techniques: Titration, Concentration Assay, Incubation

    (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding NaCl to 10 mM HEPES (pH 7.3).

    Journal: Journal of chromatography. A

    Article Title: Impact of Carrier Fluid Composition on Recovery of Nanoparticles and Proteins in Flow Field Flow Fractionation

    doi: 10.1016/j.chroma.2012.09.050

    Figure Lengend Snippet: (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding NaCl to 10 mM HEPES (pH 7.3).

    Article Snippet: All chemicals used in this study, such as hydrogen chloride (HCl), sodium hydroxide (NaOH), sodium dihydrogen phosphate (NaH2 PO4 ), disodium hydrogen phosphate (Na2 HPO4 ), the chloride salts of sodium, potassium, cesium, and magnesium (NaCl, KCl, CsCl, MgCl2 ), and the 1 M HEPES stock solution (pH 7.3), were acquired from Fisher Scientific (Pittsburgh, PA, USA).

    Techniques:

    binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .

    Journal: Physical chemistry chemical physics : PCCP

    Article Title: Cations induce shape remodeling of negatively charged phospholipid membranes

    doi: 10.1039/c7cp00718c

    Figure Lengend Snippet: binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .

    Article Snippet: CaCl2 , MgCl2 , NaCl, Casein, Tris, HEPES, DTT and EDTA were obtained from Fisher Scientific (Rochester, NY).

    Techniques: Binding Assay, Titration