Structured Review

Amresco hepes
Ni 2+ and <t>L-cys</t> binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM <t>HEPES</t> buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.
Hepes, supplied by Amresco, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hepes/product/Amresco
Average 95 stars, based on 46 article reviews
Price from $9.99 to $1999.99
hepes - by Bioz Stars, 2020-04
95/100 stars

Images

1) Product Images from "Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process"

Article Title: Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

doi: 10.1080/14756366.2018.1501044

Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.
Figure Legend Snippet: Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.

Techniques Used: Binding Assay, Spectroscopy, Titration

2) Product Images from "Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process"

Article Title: Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

doi: 10.1080/14756366.2018.1501044

Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.
Figure Legend Snippet: Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.

Techniques Used: Binding Assay, Spectroscopy, Titration

Related Articles

Positive Control:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: In addition to RH Ag, the recombinant protein ROP18 (rROP18) of T. gondii RH strain produced in our lab was also used as positive control in the last steps of the standardization. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

Enzyme-linked Immunosorbent Assay:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: Paragraph title: Western blotting assay and enzyme-linked immunosorbent assay (ELISA) ... The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Incubation:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C. .. This step was followed by incubation with horseradish peroxide (HRP)-linked secondary antibody (1:10000, anti-rabbit IgG; Millipore, USA) for 60 min at room temperature.

Activity Assay:

Article Title: Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process
Article Snippet: .. Acetohydroxamic acid (AHA; C2 H5 NO2 , purity: 98%), jack bean urease (JBU; type III with activity of 31.66 U/mg solid), urea, dithiothreitol (DTT), L-cysteine (L-cys), glutathione (GSH), sodium fluoride (NaF), and HEPES (Amresco > 99%) were purchased from Sigma Aldrich (Steineheim, Germany). .. Boric acid (BA) was purchased from Aladdin Chemistry Company.

Spectrophotometry:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: The protein quantification was performed by the Bicinchoninic Acid Protein Assay (Ref. 23227, Thermo Scientific, Rockford, IL) by using the spectrophotometer EPOCH (BioTek Instruments, Winooski, VT, USA) at 280 nm. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

BIA-KA:

Article Title: The Molecular Targets and Anti-Invasive Effects of 2,6-bis-(4-hydroxyl-3methoxybenzylidine) cyclohexanone or BHMC in MDA-MB-231 Human Breast Cancer Cells
Article Snippet: 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT), bovine serum albumin (BSA) and dimethyl sulfoxide (DMSO) were also purchased from Amresco. .. The polyvinylidene fluoride (PVDF) membrane and bicinchoninic acid (BCA) protein determination kit were purchased from Merck Milipore (Burlington, MA, USA).

Modification:

Article Title: Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis
Article Snippet: Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco, Langley, OK, USA) supplemented with 10% fetal bovine serum (Gibco, Langley, OK, USA) at 37°C in a humidified atmosphere with 5% CO2 (CO2 incubator). .. The human umbilical vein endothelial cells (HUVECs) line was cultured in DMEM supplemented with 10% FBS, hepes (3.4 g/L) (Amresco, USA) at 37°C in a humidified atmosphere with 5% CO2 .

Western Blot:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: Paragraph title: Western blotting assay and enzyme-linked immunosorbent assay (ELISA) ... The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Derivative Assay:

Article Title: Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis
Article Snippet: Cell culture The A7r5 cell line, originally derived from an embryonic rat aorta, was purchased from American Type Culture Collection (Manassas, VA, USA). .. The human umbilical vein endothelial cells (HUVECs) line was cultured in DMEM supplemented with 10% FBS, hepes (3.4 g/L) (Amresco, USA) at 37°C in a humidified atmosphere with 5% CO2 .

High Performance Liquid Chromatography:

Article Title: Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit
Article Snippet: Bovine serum albumin (BSA), human serum albumin (HSA), methyl 4-bromobutanoate, trisodium citrate dihydrate, and Tween-20 were purchased from Amresco (CochranSolon, OH, USA). .. Acetonitrile (HPLC grade) were purchased from Thermo Fisher Scientific (Waltham, NJ, USA).

Protease Inhibitor:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: Finally, 1x protease inhibitor cocktail (dilution 1:100) was added to the antigen (Ref. I3786, Sigma-Aldrich, St. Louis, USA), the aliquots were performed and stored at −80°C. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

Cell Culture:

Article Title: Molecular genetic characterization of p53 mutated oropharyngeal squamous cell carcinoma cells transformed with human papillomavirus E6 and E7 oncogenes
Article Snippet: .. The cell lines were cultured in RPMI-1640 (Welgene, Seoul, Korea) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco BRL, Grand Island, NY, USA), 1% penicillin/streptomycin (Gibco BRL), 10 mmol/l HEPES (Amresco Inc., Solon, OH, USA) at 37°C in a humidified incubator with 5% CO2 . .. Transfection with HPV16 E6 and E7 oncogenes We used both the HPV16 E6 and E7 oncogenes coding regions based on the sequences available from the HPV type 16 complete genome (GenBank: K02718.1).

Article Title: Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis
Article Snippet: .. The human umbilical vein endothelial cells (HUVECs) line was cultured in DMEM supplemented with 10% FBS, hepes (3.4 g/L) (Amresco, USA) at 37°C in a humidified atmosphere with 5% CO2 . .. The THP-1 cell line was cultured in RPMI 1640 medium containing 10% FBS at 37°C in a humidified atmosphere with 5% CO2 .

other:

Article Title: Acidosis Promotes Bcl-2 Family-mediated Evasion of Apoptosis
Article Snippet: HEPES was purchased from Amresco (Solon, OH).

Article Title: Effects of PTEN Loss and Activated KRAS Overexpression on Mechanical Properties of Breast Epithelial Cells
Article Snippet: Polyacrylamide gels were made on (3-Aminopropyl) trimethoxysilane (APTMS) (Alfa Aesar, Haverhill, MA, USA) and glutaraldehyde (Amresco, Cleveland, OH, USA) treated 25 µm × 25 µm glass slides with acrylamide (Bio-Rad, Hercules, CA, USA), bis-acrylamide (Bio-Rad), 8.2 pH HEPES buffer (Amresco), ammonium persulfate (APS) (Amresco), and tetramethylethylenediamine (TEMED) (Amresco) as previously described [ ].

Article Title: Ca2+ is a Regulator of the WNK/OSR1/NKCC Pathway in a Human Salivary Gland Cell Line
Article Snippet: KCl, EGTA, Glucose, and HEPES was from Amresco (Solon, OH, USA).

Droplet Countercurrent Chromatography:

Article Title: Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit
Article Snippet: Materials Alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), tenuazonic acid (TeA), N, N -dicyclohexylcarbodiimide (DCC), N -hydroxysuccinimide (NHS), and gold chloride (HAuCl4 ·3H2 O) were purchased from Sigma-Aldrich (Shanghai, China). .. Bovine serum albumin (BSA), human serum albumin (HSA), methyl 4-bromobutanoate, trisodium citrate dihydrate, and Tween-20 were purchased from Amresco (CochranSolon, OH, USA).

Sonication:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: The antigen was obtained after recovering the tachyzoites from the culture and centrifuged at 3,000 rpm for 5 min in RPMI medium, the tachyzoites pellet was resuspended in saline and subjected 5 times to freeze-thawing and to breakage by sonication 8 times a 20 W for 20 s. Subsequently, the lysis of the parasite was verified by microscopy. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

Recombinant:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL. .. Likewise, a lysate antigen from a Knockout strain for ROP18 (KOROP18, a gift from the Dr. Sibley, St. Louis, USA) of T. gondii was used as another negative control of the assay, this antigen was prepared similar to RH Ag.

MTT Assay:

Article Title: The Molecular Targets and Anti-Invasive Effects of 2,6-bis-(4-hydroxyl-3methoxybenzylidine) cyclohexanone or BHMC in MDA-MB-231 Human Breast Cancer Cells
Article Snippet: .. 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT), bovine serum albumin (BSA) and dimethyl sulfoxide (DMSO) were also purchased from Amresco. .. The polyvinylidene fluoride (PVDF) membrane and bicinchoninic acid (BCA) protein determination kit were purchased from Merck Milipore (Burlington, MA, USA).

Mutagenesis:

Article Title: Molecular genetic characterization of p53 mutated oropharyngeal squamous cell carcinoma cells transformed with human papillomavirus E6 and E7 oncogenes
Article Snippet: Both of the tongue cancer cell lines harbor p53 mutations, whereas the YD8 cell line has non-disruptive mutation that causes histidine to be substituted for by arginine at codon 273 in exon 8, the YD10B cell line has disruptive mutation that causes stop codon to be substitutes for tyrosine at codon 236 in exon 7 ( ). .. The cell lines were cultured in RPMI-1640 (Welgene, Seoul, Korea) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco BRL, Grand Island, NY, USA), 1% penicillin/streptomycin (Gibco BRL), 10 mmol/l HEPES (Amresco Inc., Solon, OH, USA) at 37°C in a humidified incubator with 5% CO2 .

Bicinchoninic Acid Protein Assay:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: The protein quantification was performed by the Bicinchoninic Acid Protein Assay (Ref. 23227, Thermo Scientific, Rockford, IL) by using the spectrophotometer EPOCH (BioTek Instruments, Winooski, VT, USA) at 280 nm. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

Microscopy:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: The antigen was obtained after recovering the tachyzoites from the culture and centrifuged at 3,000 rpm for 5 min in RPMI medium, the tachyzoites pellet was resuspended in saline and subjected 5 times to freeze-thawing and to breakage by sonication 8 times a 20 W for 20 s. Subsequently, the lysis of the parasite was verified by microscopy. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

Purification:

Article Title: Host Plant Effects on Alkaline Phosphatase Activity in the Whiteflies, Bemisia tabaci Biotype B and Trialeurodes vaporariorum
Article Snippet: .. Chemicals 2-(N-morpholino) ethanesulfonic acid (MES), N-tris (hydro xymethyl) methyl-3-aminopropanesulfonic acid (TAPS) were purchased from Sigma ( www.sigmaaldrich.com ), N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid (TAPS), 2-N- cyclohexylamino ethanesulfonic acid (CHES), 3-(cyclohexy lamino)-1-propanesulfonic acid (CAPS), Purified bovine serum albumin (BSA), and p -nitrophenyl phosphate (pNPP) were obtained from Amresco ( www.amresco.com ). ..

Protein Extraction:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: The sciatic nerve at the chronic constriction injury site (at the site with four chromic gut ligatures) and the right quarter part of the L4-L6 segments of the spinal cord were quickly separated and collected in a tissue lysis buffer (T-PER® Tissue Protein Extraction Reagent, Thermo Scientific, USA) and a “cocktail” of protease inhibitors (Roche, Germany) and phosphatase inhibitor (Roche, Germany). .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Polyacrylamide Gel Electrophoresis:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: Equal protein loading from the dorsal horn and injured sciatic nerve was verified using Coomassie brilliant blue staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Lysis:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: The antigen was obtained after recovering the tachyzoites from the culture and centrifuged at 3,000 rpm for 5 min in RPMI medium, the tachyzoites pellet was resuspended in saline and subjected 5 times to freeze-thawing and to breakage by sonication 8 times a 20 W for 20 s. Subsequently, the lysis of the parasite was verified by microscopy. .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL.

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: The sciatic nerve at the chronic constriction injury site (at the site with four chromic gut ligatures) and the right quarter part of the L4-L6 segments of the spinal cord were quickly separated and collected in a tissue lysis buffer (T-PER® Tissue Protein Extraction Reagent, Thermo Scientific, USA) and a “cocktail” of protease inhibitors (Roche, Germany) and phosphatase inhibitor (Roche, Germany). .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

SDS Page:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: Protein samples were separated by SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes. .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Negative Control:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL. .. Likewise, a lysate antigen from a Knockout strain for ROP18 (KOROP18, a gift from the Dr. Sibley, St. Louis, USA) of T. gondii was used as another negative control of the assay, this antigen was prepared similar to RH Ag.

Homogenization:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: After homogenization, the tissue lysates were centrifuged at 13,500 rpm for 45 min at 4 °C. .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Knock-Out:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL. .. Likewise, a lysate antigen from a Knockout strain for ROP18 (KOROP18, a gift from the Dr. Sibley, St. Louis, USA) of T. gondii was used as another negative control of the assay, this antigen was prepared similar to RH Ag.

Produced:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL. .. Likewise, a lysate antigen from a Knockout strain for ROP18 (KOROP18, a gift from the Dr. Sibley, St. Louis, USA) of T. gondii was used as another negative control of the assay, this antigen was prepared similar to RH Ag.

Concentration Assay:

Article Title: Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum
Article Snippet: .. In the same way, three negative controls were included: the recombinant protein Disulfide isomerase of T. gondii (PDI); Lucifensin-CPD, a recombinant protein from the fly Lucilia sericata (LucGT), both produced in our lab, and bovine serum albumin (BSA) (AMRESCO), these controls were used at a concentration of 6.25 μg/mL. .. Likewise, a lysate antigen from a Knockout strain for ROP18 (KOROP18, a gift from the Dr. Sibley, St. Louis, USA) of T. gondii was used as another negative control of the assay, this antigen was prepared similar to RH Ag.

Staining:

Article Title: An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve
Article Snippet: Equal protein loading from the dorsal horn and injured sciatic nerve was verified using Coomassie brilliant blue staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). .. The blots were blocked using 5% bovine serum albumin (BSA) (Amresco, USA) in Tris-buffered saline (TBS) with 0.1% Tween-20 overnight at 4 °C and then incubated with anti-phospho-p38 (1:1000; Cell Signaling Technology, USA), p38 (1:1000; Cell Signaling Technology, USA), MOR-1 (receptor for β-endorphin; 1:200; Santa Cruz Biotechnology, USA), or GAPDH (internal control; 1:6000; Cell Signaling Technology, USA) primary antibody in TBS containing 0.1% Tween-20 overnight at 4 °C.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Amresco hepes
    Ni 2+ and <t>L-cys</t> binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM <t>HEPES</t> buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.
    Hepes, supplied by Amresco, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Amresco
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process

    doi: 10.1080/14756366.2018.1501044

    Figure Lengend Snippet: Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.

    Article Snippet: Acetohydroxamic acid (AHA; C2 H5 NO2 , purity: 98%), jack bean urease (JBU; type III with activity of 31.66 U/mg solid), urea, dithiothreitol (DTT), L-cysteine (L-cys), glutathione (GSH), sodium fluoride (NaF), and HEPES (Amresco > 99%) were purchased from Sigma Aldrich (Steineheim, Germany).

    Techniques: Binding Assay, Spectroscopy, Titration

    Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Coptisine-induced inhibition of Helicobacter pylori: elucidation of specific mechanisms by probing urease active site and its maturation process

    doi: 10.1080/14756366.2018.1501044

    Figure Lengend Snippet: Ni 2+ and L-cys binding to coptisine (Cop) measured using UV-visible spectroscopy. 16 μM Cop in the absence or presence of different molar equivalents of Ni 2+ (1 mM NiCl 2 ) (A) or L-cys (1 mM) (B) in 20 mM HEPES buffer, pH 7.5. A-F, 0.0 to 320 μM of Ni 2+ ; A-F, 0.0 to 200 μM of L-cys. The inset shows the titration curves for binding of Ni 2+ or L-cys to Cop at 355 nm. Wavelength (nm) is represented on the x -axis and absorption (Abs) is represented on the y -axis.

    Article Snippet: Acetohydroxamic acid (AHA; C2 H5 NO2 , purity: 98%), jack bean urease (JBU; type III with activity of 31.66 U/mg solid), urea, dithiothreitol (DTT), L-cysteine (L-cys), glutathione (GSH), sodium fluoride (NaF), and HEPES (Amresco > 99%) were purchased from Sigma Aldrich (Steineheim, Germany).

    Techniques: Binding Assay, Spectroscopy, Titration