biotin x dhpe lipids  (Thermo Fisher)


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    Name:
    Biotin X DHPE N 6 Biotinoyl amino hexanoyl 1 2 Dihexadecanoyl sn Glycero 3 Phosphoethanolamine Triethylammonium Salt
    Description:
    The biotinylated phospholipid biotin X DHPE can be used to couple avidin or streptavidin to cell membranes liposomes and lipoid monolayers
    Catalog Number:
    b1616
    Price:
    None
    Applications:
    Cell Analysis|Cellular Imaging|Immunofluorescence (IF)|Immunofluorescence Staining & Detection
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher biotin x dhpe lipids
    Single <t>Biotin-X-DHPE</t> molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.
    The biotinylated phospholipid biotin X DHPE can be used to couple avidin or streptavidin to cell membranes liposomes and lipoid monolayers
    https://www.bioz.com/result/biotin x dhpe lipids/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin x dhpe lipids - by Bioz Stars, 2020-02
    90/100 stars

    Related Products / Commonly Used Together

    alexa fluor 546

    Images

    1) Product Images from "Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature"

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature

    Journal: Membranes

    doi: 10.3390/membranes7010015

    Single Biotin-X-DHPE molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.
    Figure Legend Snippet: Single Biotin-X-DHPE molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.

    Techniques Used: Diffusion-based Assay

    Related Articles

    Centrifugation:

    Article Title: Surface Characterization of Bovine Milk Phospholipid Monolayers by Langmuir Isotherms and Microscopic Techniques
    Article Snippet: The head group-labeled fluorescent phospholipid analog Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red®-DHPE, 0.1 mL/mL in chloroform, Invitrogen, Molecular Probes (Carlsbad, CA)) was used to provide contrast between lipid phases. .. Raw cream was obtained by centrifugation of raw milk at 3000 g for 5 minutes at 20°C (Eppendorf Centrifuge 5810 R).

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: Cells were pelleted by centrifugation (900 rcf × 5 min), washed twice with RPMI-1640 SFM to remove secreted antibodies, and adjusted to a cell density of 2 × 106 cells/mL. .. For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen).

    Immunocytochemistry:

    Article Title: The Nucleoside Diphosphate Kinase D (NM23-H4) Binds the Inner Mitochondrial Membrane with High Affinity to Cardiolipin and Couples Nucleotide Transfer with Respiration *
    Article Snippet: For immunocytochemistry and immunoprecipitation experiments, we used antibodies which were affinity-purified using recombinant NDPK-D. .. The mitochondria-selective dye MitoTracker TM Red CMXRos, AlexaFluor 488-conjugated goat anti-rabbit IgG, and Biotin-X-DHPE were from Molecular Probes.

    Filtration:

    Article Title: Surface Characterization of Bovine Milk Phospholipid Monolayers by Langmuir Isotherms and Microscopic Techniques
    Article Snippet: Milli-Q water (18 MΩ.cm), used as trough sub-phase, was purified by means of a Millipore filtration device (Millipore, Milli Q™). .. The head group-labeled fluorescent phospholipid analog Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red®-DHPE, 0.1 mL/mL in chloroform, Invitrogen, Molecular Probes (Carlsbad, CA)) was used to provide contrast between lipid phases.

    Synthesized:

    Article Title: Diffusion as a Probe of the Heterogeneity of Antimicrobial Peptide-Membrane Interactions †
    Article Snippet: Mag2 and mpX were synthesized using the standard fluoren-9-ylmethoxycarbonyl (Fmoc)-based solid-phase method on a PS3 peptide synthesizer (Protein Technologies, MA), purified by reverse-phase chromatography, and verified by matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. .. Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (referred to hereafter as TR-DHPE) was purchased from Molecular Probe (Eugene, OR).

    Electrophoresis:

    Article Title: In vivo siRNA delivery system for targeting to the liver by poly-l-glutamic acid-coated lipoplex
    Article Snippet: For preparation of rhodamine-labeled cationic liposome, Lissamine™ rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine-DHPE, Invitrogen, Carlsbad, CA, USA) was incorporated at 1?mol% into the total lipids. .. The particle size and ?-potential of cationic liposomes were measured by dynamic light-scattering and electrophoresis light-scattering methods, respectively (ELS-Z2, Otsuka Electronics Co., Ltd., Osaka, Japan).

    Incubation:

    Article Title: Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel
    Article Snippet: Liposomes consisted of 4∶4∶2∶0.1∶2×10−4 ratio of 1,2,dioleoyl-sn-glycero-3-phosphocho- line (DOPC) (Avanti Polar Lipids), 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (POPC) (Avanti Polar Lipids), cholesterol (Avanti Polar Lipids), bovine brain disialoganglioside GD1a (Sigma), and N-((6-(biotinoyl)amino)hexanoyl)-1,2- dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE) (Molecular Probes). .. We mounted a flow cell on the microscope stage and loaded liposomes at the rate of 0.04 ml/min for 1 min, after which the flow was stopped and the liposomes incubated for a minimum of 15 min.

    Article Title: Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
    Article Snippet: Biotinylated DHPE (Molecular Probes) or biotinylated α-GalCer, (2S, 3S, 4R)-1- O -(α- d -galactopyranosyl)-2-[ N -2-{2-[2-(2-biotinylamino-ethoxy)-ethoxy]-ethoxymethoxy}-tetracosanoylamino]-1,3,4-octadecantriol, was sonicated in TBS (pH 5.5) in the presence or absence of detergent or saposins at the indicated concentrations, and soluble CD1d, purified as previously described , was added. .. To examine the effect of saposins on preloaded lipids, CD1d was incubated with biotinylated lipid in the presence of 0.5% CHAPS and isolated by using neutravidin-agarose beads as described above.

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen). .. The cells were washed and incubated for 20 min with 10 μ M L-RB in Dulbecco’s modified Eagles medium containing 25 mM HEPES (Invitrogen) on a rotating shaker at room temperature.

    Mass Spectrometry:

    Article Title: Diffusion as a Probe of the Heterogeneity of Antimicrobial Peptide-Membrane Interactions †
    Article Snippet: Mag2 and mpX were synthesized using the standard fluoren-9-ylmethoxycarbonyl (Fmoc)-based solid-phase method on a PS3 peptide synthesizer (Protein Technologies, MA), purified by reverse-phase chromatography, and verified by matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. .. Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (referred to hereafter as TR-DHPE) was purchased from Molecular Probe (Eugene, OR).

    Modification:

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen). .. The cells were washed and incubated for 20 min with 10 μ M L-RB in Dulbecco’s modified Eagles medium containing 25 mM HEPES (Invitrogen) on a rotating shaker at room temperature.

    High Performance Liquid Chromatography:

    Article Title: Surface Characterization of Bovine Milk Phospholipid Monolayers by Langmuir Isotherms and Microscopic Techniques
    Article Snippet: All solvents (chloroform, methanol, hexane and diethyl ether) were from Fisher Scientific (Pittsburgh, PA) and were of HPLC grade. .. The head group-labeled fluorescent phospholipid analog Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red®-DHPE, 0.1 mL/mL in chloroform, Invitrogen, Molecular Probes (Carlsbad, CA)) was used to provide contrast between lipid phases.

    Conjugation Assay:

    Article Title: Multi-color single-molecule tracking and subtrajectory analysis for quantification of spatiotemporal dynamics and kinetics upon T cell activation
    Article Snippet: N -((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn -glycero-3-phosphoethanolamine (Biotin-X-DHPE) and streptavidin were purchased from Invitrogen (Japan). .. Qdot 655 and Qdot 585 were conjugated to anti-CD3ε and -CD45 antibodies, respectively, using the Qdot Antibody Conjugation Kit (Invitrogen) according to the manufacturer’s instructions.

    Flow Cytometry:

    Article Title: Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel
    Article Snippet: Liposomes consisted of 4∶4∶2∶0.1∶2×10−4 ratio of 1,2,dioleoyl-sn-glycero-3-phosphocho- line (DOPC) (Avanti Polar Lipids), 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (POPC) (Avanti Polar Lipids), cholesterol (Avanti Polar Lipids), bovine brain disialoganglioside GD1a (Sigma), and N-((6-(biotinoyl)amino)hexanoyl)-1,2- dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE) (Molecular Probes). .. We mounted a flow cell on the microscope stage and loaded liposomes at the rate of 0.04 ml/min for 1 min, after which the flow was stopped and the liposomes incubated for a minimum of 15 min.

    Concentration Assay:

    Article Title: Single-particle kinetics of influenza virus membrane fusion
    Article Snippet: .. A solution was prepared with a 4:4:2:0.1:5 × 10−5 ratio of 1,2,dioleoyl- sn -glycero-3-phosphocholine (DOPC) (Avanti Polar Lipids), 1-oleoyl-2-palmitoyl- sn -glycero-3-phosphocholine (POPC) (Avanti Polar Lipids), cholesterol (Avanti Polar Lipids), bovine brain disialoganglioside GD1a (Sigma), and N -((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (biotin-X DHPE) (Molecular Probes) in HNE (5 mM Hepes, 145 mM NaCl, 0.2 mM EDTA) buffer at a concentration of ≈10 g/liter. ..

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546). .. The concentration of labeled lipid used was determined by the amount needed for FRAP experiments on MB-DHPE and then kept constant for all samples.

    Cell Culture:

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: Paragraph title: Hybridoma cell culturing and labeling ... For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen).

    SDS Page:

    Article Title: Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
    Article Snippet: Biotinylated DHPE (Molecular Probes) or biotinylated α-GalCer, (2S, 3S, 4R)-1- O -(α- d -galactopyranosyl)-2-[ N -2-{2-[2-(2-biotinylamino-ethoxy)-ethoxy]-ethoxymethoxy}-tetracosanoylamino]-1,3,4-octadecantriol, was sonicated in TBS (pH 5.5) in the presence or absence of detergent or saposins at the indicated concentrations, and soluble CD1d, purified as previously described , was added. .. After 1 h at 4°C and washing, the bound proteins were separated by SDS/PAGE, and CD1d binding was analyzed by immunoblotting with the anti-CD1d antibody, D5.

    other:

    Article Title: Heterogeneous Diffusion of a Membrane-Bound pHLIP Peptide
    Article Snippet: Texas Red 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (Texas-Red-DHPE) was purchased from Molecular Probes (Eugene, OR).

    Article Title: Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
    Article Snippet: N-(dimethylaminonaphthalene-1-sulfonyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (dansyl DHPE) was from Molecular Probes.

    Article Title: A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation
    Article Snippet: Texas Red - coupled DHPE was purchased from Invitrogen.

    Article Title: Charging the Quantum Capacitance of Graphene with a Single Biological Ion Channel
    Article Snippet: 1,2-Dihexadecanoyl-sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) was from Life Technologies.

    Imaging:

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: .. For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen). .. The cells were washed and incubated for 20 min with 10 μ M L-RB in Dulbecco’s modified Eagles medium containing 25 mM HEPES (Invitrogen) on a rotating shaker at room temperature.

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: In single molecule imaging measurements, the lipid bilayers contained POPC (97.9%), MB-DHPE (2%) and biotin-X-DHPE (0.1%). .. The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546).

    Sonication:

    Article Title: Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
    Article Snippet: .. Biotinylated DHPE (Molecular Probes) or biotinylated α-GalCer, (2S, 3S, 4R)-1- O -(α- d -galactopyranosyl)-2-[ N -2-{2-[2-(2-biotinylamino-ethoxy)-ethoxy]-ethoxymethoxy}-tetracosanoylamino]-1,3,4-octadecantriol, was sonicated in TBS (pH 5.5) in the presence or absence of detergent or saposins at the indicated concentrations, and soluble CD1d, purified as previously described , was added. .. After 1 h at 37°C, the samples were neutralized, diluted in TBS (pH 7.4), and neutravidin-agarose beads added (Pierce, Rockford, IL).

    Affinity Purification:

    Article Title: The Nucleoside Diphosphate Kinase D (NM23-H4) Binds the Inner Mitochondrial Membrane with High Affinity to Cardiolipin and Couples Nucleotide Transfer with Respiration *
    Article Snippet: The affinity-purified antibodies against NDPK-A and -B were prepared using the recombinant proteins as described ( ). .. The mitochondria-selective dye MitoTracker TM Red CMXRos, AlexaFluor 488-conjugated goat anti-rabbit IgG, and Biotin-X-DHPE were from Molecular Probes.

    Binding Assay:

    Article Title: Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
    Article Snippet: Biotinylated DHPE (Molecular Probes) or biotinylated α-GalCer, (2S, 3S, 4R)-1- O -(α- d -galactopyranosyl)-2-[ N -2-{2-[2-(2-biotinylamino-ethoxy)-ethoxy]-ethoxymethoxy}-tetracosanoylamino]-1,3,4-octadecantriol, was sonicated in TBS (pH 5.5) in the presence or absence of detergent or saposins at the indicated concentrations, and soluble CD1d, purified as previously described , was added. .. After 1 h at 4°C and washing, the bound proteins were separated by SDS/PAGE, and CD1d binding was analyzed by immunoblotting with the anti-CD1d antibody, D5.

    Fluorescence:

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: .. For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen). .. The cells were washed and incubated for 20 min with 10 μ M L-RB in Dulbecco’s modified Eagles medium containing 25 mM HEPES (Invitrogen) on a rotating shaker at room temperature.

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: Membrane fluidity was tested with FRAP, using Fl-DHPE or Fl-HDA, or by imaging single molecule motion using total internal reflection fluorescence microscopy. .. The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546).

    Magnetic Beads:

    Article Title: The Nucleoside Diphosphate Kinase D (NM23-H4) Binds the Inner Mitochondrial Membrane with High Affinity to Cardiolipin and Couples Nucleotide Transfer with Respiration *
    Article Snippet: The mitochondria-selective dye MitoTracker TM Red CMXRos, AlexaFluor 488-conjugated goat anti-rabbit IgG, and Biotin-X-DHPE were from Molecular Probes. .. The magnetic beads coated with sheep anti-rabbit IgG (Dynabeads® M-280) were from Invitrogen.

    Isolation:

    Article Title: Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
    Article Snippet: Biotinylated DHPE (Molecular Probes) or biotinylated α-GalCer, (2S, 3S, 4R)-1- O -(α- d -galactopyranosyl)-2-[ N -2-{2-[2-(2-biotinylamino-ethoxy)-ethoxy]-ethoxymethoxy}-tetracosanoylamino]-1,3,4-octadecantriol, was sonicated in TBS (pH 5.5) in the presence or absence of detergent or saposins at the indicated concentrations, and soluble CD1d, purified as previously described , was added. .. To examine the effect of saposins on preloaded lipids, CD1d was incubated with biotinylated lipid in the presence of 0.5% CHAPS and isolated by using neutravidin-agarose beads as described above.

    Avidin-Biotin Assay:

    Article Title: Biochemical characterization of hyperactive ?2-chimaerin mutants revealed an enhanced exposure of C1 and Rac-GAP domains †
    Article Snippet: L-α-phosphatidylcholine, 1,2-diacyl-sn-glycero-3-phospho-L-serine, and avidin high capacity agarose were obtained from Sigma. .. Biotin-X-DHPE was purchased from Molecular Probes (Eugene, Oregon).

    Microscopy:

    Article Title: Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel
    Article Snippet: Liposomes consisted of 4∶4∶2∶0.1∶2×10−4 ratio of 1,2,dioleoyl-sn-glycero-3-phosphocho- line (DOPC) (Avanti Polar Lipids), 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (POPC) (Avanti Polar Lipids), cholesterol (Avanti Polar Lipids), bovine brain disialoganglioside GD1a (Sigma), and N-((6-(biotinoyl)amino)hexanoyl)-1,2- dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE) (Molecular Probes). .. We mounted a flow cell on the microscope stage and loaded liposomes at the rate of 0.04 ml/min for 1 min, after which the flow was stopped and the liposomes incubated for a minimum of 15 min.

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: Membrane fluidity was tested with FRAP, using Fl-DHPE or Fl-HDA, or by imaging single molecule motion using total internal reflection fluorescence microscopy. .. The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546).

    Purification:

    Article Title: Surface Characterization of Bovine Milk Phospholipid Monolayers by Langmuir Isotherms and Microscopic Techniques
    Article Snippet: Milli-Q water (18 MΩ.cm), used as trough sub-phase, was purified by means of a Millipore filtration device (Millipore, Milli Q™). .. The head group-labeled fluorescent phospholipid analog Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red®-DHPE, 0.1 mL/mL in chloroform, Invitrogen, Molecular Probes (Carlsbad, CA)) was used to provide contrast between lipid phases.

    Article Title: Diffusion as a Probe of the Heterogeneity of Antimicrobial Peptide-Membrane Interactions †
    Article Snippet: Mag2 and mpX were synthesized using the standard fluoren-9-ylmethoxycarbonyl (Fmoc)-based solid-phase method on a PS3 peptide synthesizer (Protein Technologies, MA), purified by reverse-phase chromatography, and verified by matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. .. Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (referred to hereafter as TR-DHPE) was purchased from Molecular Probe (Eugene, OR).

    Article Title: Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
    Article Snippet: .. Biotinylated DHPE (Molecular Probes) or biotinylated α-GalCer, (2S, 3S, 4R)-1- O -(α- d -galactopyranosyl)-2-[ N -2-{2-[2-(2-biotinylamino-ethoxy)-ethoxy]-ethoxymethoxy}-tetracosanoylamino]-1,3,4-octadecantriol, was sonicated in TBS (pH 5.5) in the presence or absence of detergent or saposins at the indicated concentrations, and soluble CD1d, purified as previously described , was added. .. After 1 h at 37°C, the samples were neutralized, diluted in TBS (pH 7.4), and neutravidin-agarose beads added (Pierce, Rockford, IL).

    Labeling:

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: .. For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen). .. The cells were washed and incubated for 20 min with 10 μ M L-RB in Dulbecco’s modified Eagles medium containing 25 mM HEPES (Invitrogen) on a rotating shaker at room temperature.

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: .. The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546). .. The concentration of labeled lipid used was determined by the amount needed for FRAP experiments on MB-DHPE and then kept constant for all samples.

    Confocal Microscopy:

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: Confocal microscopy was performed to measure colocalization between nanoparticles and lipids. .. The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546).

    Injection:

    Article Title: Quantitative Imaging of Protein Secretions from Single Cells in Real Time
    Article Snippet: For imaging, 75 μ L of the cell solution was manually injected into the imaging chamber. .. For fluorescence imaging, the plasma membrane of live cells were labeled with the membrane-localizing dye, Lissamine rhodamine B 1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine DHPE) (Ex, 560 nm/Em, 580 nm) (L-RB, Invitrogen).

    Recombinant:

    Article Title: The Nucleoside Diphosphate Kinase D (NM23-H4) Binds the Inner Mitochondrial Membrane with High Affinity to Cardiolipin and Couples Nucleotide Transfer with Respiration *
    Article Snippet: The affinity-purified antibodies against NDPK-A and -B were prepared using the recombinant proteins as described ( ). .. The mitochondria-selective dye MitoTracker TM Red CMXRos, AlexaFluor 488-conjugated goat anti-rabbit IgG, and Biotin-X-DHPE were from Molecular Probes.

    In Situ:

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature
    Article Snippet: .. The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546). .. The concentration of labeled lipid used was determined by the amount needed for FRAP experiments on MB-DHPE and then kept constant for all samples.

    Immunoprecipitation:

    Article Title: The Nucleoside Diphosphate Kinase D (NM23-H4) Binds the Inner Mitochondrial Membrane with High Affinity to Cardiolipin and Couples Nucleotide Transfer with Respiration *
    Article Snippet: For immunocytochemistry and immunoprecipitation experiments, we used antibodies which were affinity-purified using recombinant NDPK-D. .. The mitochondria-selective dye MitoTracker TM Red CMXRos, AlexaFluor 488-conjugated goat anti-rabbit IgG, and Biotin-X-DHPE were from Molecular Probes.

    Reversed-phase Chromatography:

    Article Title: Diffusion as a Probe of the Heterogeneity of Antimicrobial Peptide-Membrane Interactions †
    Article Snippet: Mag2 and mpX were synthesized using the standard fluoren-9-ylmethoxycarbonyl (Fmoc)-based solid-phase method on a PS3 peptide synthesizer (Protein Technologies, MA), purified by reverse-phase chromatography, and verified by matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. .. Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (referred to hereafter as TR-DHPE) was purchased from Molecular Probe (Eugene, OR).

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  • 90
    Thermo Fisher biotin x dhpe lipids
    Single <t>Biotin-X-DHPE</t> molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.
    Biotin X Dhpe Lipids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher membrane impermeable sulfosuccinimidobiotin
    Single <t>Biotin-X-DHPE</t> molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.
    Membrane Impermeable Sulfosuccinimidobiotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher biotinylated kras peptide
    Single <t>Biotin-X-DHPE</t> molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.
    Biotinylated Kras Peptide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Single Biotin-X-DHPE molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.

    Journal: Membranes

    Article Title: Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature

    doi: 10.3390/membranes7010015

    Figure Lengend Snippet: Single Biotin-X-DHPE molecules tagged with Streptavidin-Alexa546 were tracked on the curved supported lipid bilayers (ROC = 28 nm) in space and time. ( a ) example trajectories (blue) show the heterogeneous dynamics observed on both flat (white) and curved (black) regions. Scale bar = 1 µm; ( b ) the average step a molecule takes over 0.228 s (5 frames) when starting at a region of curvature (white) or at a flat region (grey); ( c ) the distribution of steps observed at curved and flat regions (shown in Figure S3 ) was fitted to Equation 3 to obtain the diffusion coefficients and the percentage of steps moving at that rate. The average D is plotted for t = 0.091, 0.228 and 0.456. Note that there is no fast component for the tracks that start at regions of curvature.

    Article Snippet: The dynamics of Biotin-X-DHPE lipids were detected by in situ labeling with Alexa Fluor 546 (Thermo Fisher) conjugated to streptavidin (Strep-546).

    Techniques: Diffusion-based Assay