n α acetyl l arginine  (Millipore)


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  • 86

    Structured Review

    Millipore n α acetyl l arginine
    N α Acetyl L Arginine, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n α acetyl l arginine/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n α acetyl l arginine - by Bioz Stars, 2020-04
    86/100 stars

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    Incubation:

    Article Title: Comparison of Cationic and Amphipathic Cell Penetrating Peptides for siRNA Delivery and Efficacy
    Article Snippet: After mixing the peptides with PQ, the reaction was initiated with the addition of 2 N NaOH, and the samples were incubated for 1 h at 60°C. .. N α -acetyl-L-arginine (Sigma-Aldrich) was used to produce a standard curve to quantify the guanidine groups.

    other:

    Article Title: Formiminoglutamase from Trypanosoma cruzi is an Arginase-Like Manganese Metalloenzyme
    Article Snippet: Dipicolinic acid, manganese(II) chloride tetrahydrate (≥99%), cobalt chloride hexahydrate ( > 99%), nickel chloride hexahydrate, L-arginine, α-isonitrosopropiophenone, 3-guanidinopropionic acid, 4-guanidinobuytric acid, agmatine sulfate salt, L-2-amino-3-guanidinopropionic acid hydrochloride, N α -acetyl-L-arginine, L-homoarginine hydrochloride, potassium ferricyanide and sodium nitroprusside dihydrate were purchased from Sigma.

    Fluorescence:

    Article Title: Comparison of Cationic and Amphipathic Cell Penetrating Peptides for siRNA Delivery and Efficacy
    Article Snippet: 1.2 N HCl was then added to stop the reaction, and the fluorescence was measured at excitation 312 nm and emission 395 nm using a fluorescence spectrophotometer (Hitachi, Tokyo, Japan). .. N α -acetyl-L-arginine (Sigma-Aldrich) was used to produce a standard curve to quantify the guanidine groups.

    Spectrophotometry:

    Article Title: Comparison of Cationic and Amphipathic Cell Penetrating Peptides for siRNA Delivery and Efficacy
    Article Snippet: 1.2 N HCl was then added to stop the reaction, and the fluorescence was measured at excitation 312 nm and emission 395 nm using a fluorescence spectrophotometer (Hitachi, Tokyo, Japan). .. N α -acetyl-L-arginine (Sigma-Aldrich) was used to produce a standard curve to quantify the guanidine groups.

    Trinitrobenzene Sulfonic Acid Assay:

    Article Title: Comparison of Cationic and Amphipathic Cell Penetrating Peptides for siRNA Delivery and Efficacy
    Article Snippet: N α -acetyl-L-arginine (Sigma-Aldrich) was used to produce a standard curve to quantify the guanidine groups. .. TNBSA assay was performed to measure the presence of free K21 amines.

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    Millipore bapna tryptase substrate
    Measurement of β-hexosaminidase and tryptase in the supernatant of <t>RBL-2H3</t> mast cells stimulated with calcium ionophore A23187. RBL-2H3 cells were incubated for one hour with A23187 ionophore in modified Tyrodes buffer, and degranulation was quantified after incubation with (A) 4-Methylumbelliferone (4MU) substrate for β-hexosaminidase or with (B) <t>BAPNA</t> substrate for tryptase. Data are shown from one representative experiment; values represent means ± SD of triplicate samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s post test. ** p
    Bapna Tryptase Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bapna tryptase substrate/product/Millipore
    Average 80 stars, based on 1 article reviews
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    85
    Millipore β secretase h2 n arg glu edans glu val asn leu asp ala glu phe lys dabcyl arg o
    Measurement of β-hexosaminidase and tryptase in the supernatant of <t>RBL-2H3</t> mast cells stimulated with calcium ionophore A23187. RBL-2H3 cells were incubated for one hour with A23187 ionophore in modified Tyrodes buffer, and degranulation was quantified after incubation with (A) 4-Methylumbelliferone (4MU) substrate for β-hexosaminidase or with (B) <t>BAPNA</t> substrate for tryptase. Data are shown from one representative experiment; values represent means ± SD of triplicate samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s post test. ** p
    β Secretase H2 N Arg Glu Edans Glu Val Asn Leu Asp Ala Glu Phe Lys Dabcyl Arg O, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β secretase h2 n arg glu edans glu val asn leu asp ala glu phe lys dabcyl arg o/product/Millipore
    Average 85 stars, based on 1 article reviews
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    β secretase h2 n arg glu edans glu val asn leu asp ala glu phe lys dabcyl arg o - by Bioz Stars, 2020-04
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    85
    Millipore bapna assay
    Measurement of β-hexosaminidase and tryptase in the supernatant of <t>RBL-2H3</t> mast cells stimulated with calcium ionophore A23187. RBL-2H3 cells were incubated for one hour with A23187 ionophore in modified Tyrodes buffer, and degranulation was quantified after incubation with (A) 4-Methylumbelliferone (4MU) substrate for β-hexosaminidase or with (B) <t>BAPNA</t> substrate for tryptase. Data are shown from one representative experiment; values represent means ± SD of triplicate samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s post test. ** p
    Bapna Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bapna assay/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bapna assay - by Bioz Stars, 2020-04
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    94
    Millipore baee
    Measurement of β-hexosaminidase and tryptase in the supernatant of <t>RBL-2H3</t> mast cells stimulated with calcium ionophore A23187. RBL-2H3 cells were incubated for one hour with A23187 ionophore in modified Tyrodes buffer, and degranulation was quantified after incubation with (A) 4-Methylumbelliferone (4MU) substrate for β-hexosaminidase or with (B) <t>BAPNA</t> substrate for tryptase. Data are shown from one representative experiment; values represent means ± SD of triplicate samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s post test. ** p
    Baee, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    Image Search Results


    Measurement of β-hexosaminidase and tryptase in the supernatant of RBL-2H3 mast cells stimulated with calcium ionophore A23187. RBL-2H3 cells were incubated for one hour with A23187 ionophore in modified Tyrodes buffer, and degranulation was quantified after incubation with (A) 4-Methylumbelliferone (4MU) substrate for β-hexosaminidase or with (B) BAPNA substrate for tryptase. Data are shown from one representative experiment; values represent means ± SD of triplicate samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s post test. ** p

    Journal: Journal of applied toxicology : JAT

    Article Title: Searching for Tryptase in the RBL-2H3 Mast Cell Model: Preparation for Comparative Mast Cell Toxicology Studies with Zebrafish

    doi: 10.1002/jat.3738

    Figure Lengend Snippet: Measurement of β-hexosaminidase and tryptase in the supernatant of RBL-2H3 mast cells stimulated with calcium ionophore A23187. RBL-2H3 cells were incubated for one hour with A23187 ionophore in modified Tyrodes buffer, and degranulation was quantified after incubation with (A) 4-Methylumbelliferone (4MU) substrate for β-hexosaminidase or with (B) BAPNA substrate for tryptase. Data are shown from one representative experiment; values represent means ± SD of triplicate samples. Statistical significance was determined by one-way ANOVA followed by Tukey’s post test. ** p

    Article Snippet: After observing no tryptase detection from degranulated RBL-2H3 cells using the BAPNA tryptase substrate, we utilized the commercially available tryptase degranulation kit from EMD Millipore, which contains another known tryptase substrate: tosyl-Gly-Pro-Lys-pNA, instead of BAPNA.

    Techniques: Incubation, Modification