Journal: Molecular Metabolism

Article Title: Training-induced plasma miR-29a-3p is secreted by skeletal muscle and contributes to metabolic adaptations to resistance exercise in mice

doi: 10.1016/j.molmet.2025.102173

Figure Lengend Snippet: In vitro study of the impact of EPS in miRNA secretion and the modulatory effect of miR-29a-3p inhibition in training-associated miRNAs in mouse myotubes. A . Schematic representation of electrostimulation protocol on C2C12 cells and isolation of miRNAs from EVs and cells. Created with BioRender. B-D. Relative expression levels of miR-30a-5p, let-7f-5p, and miR-29a-3p in cells and EVs at 0, 3, and 20 h after electrostimulation in non-electrostimulated (C, n = 4) and electrostimulated (EPS, n = 4) cells. E-G. Relative expression of cluster 1 miRNAs ( E ), let-7i-5p ( F ), and miR-143-3p ( G ) in C2C12 myotubes where miR-29a-3p expression was inhibited by 70% (miR29dKD, n = 4) and in control myotubes (Control, n = 4). H–I. Relative expression of miR-30a-5p and miR-143-3p in non-electrostimulated (C, n = 4) and electrostimulated (EPS, n = 4) miR-29a-3p dKD cells at 0, 3, and 20 h after electrostimulation. Statistical significance was determined using unpaired, two-tailed Student’s t -test and p -values are indicated in the figures. Data are represented as mean ± SEM. Each dot represents the mean technical duplicate of each cell replicate.

Article Snippet: The mouse skeletal muscle myoblast cell line C2C12 were obtained from the American Type Culture Collection (ATCC CRL-1772).

Techniques: In Vitro, Inhibition, Isolation, Expressing, Control, Two Tailed Test

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effect of m 6 A Recognition Protein YTHDC1 on Skeletal Muscle Growth

doi: 10.3390/ani15131978

Figure Lengend Snippet: Effect of YTHDC1 on C2C12 cell proliferation as detected by real-time fluorescent quantitative PCR, Western blotting, and EdU assays. ( A ) Relative mRNA expression of interference YTHDC1. ( B ) Relative mRNA expression of overexpression YTHDC1. ( C ) Relative protein expression of interference YTHDC1. ( D ) Relative protein expression of overexpression YTHDC1. ( E ) EdU-positive cells of interference YTHDC1. ( F ) EdU-positive cells of overexpression YTHDC1. The scale is 100 μm; * p < 0.05; ** p < 0.01.

Article Snippet: Mouse C2C12 myoblast cells were cultured in DMEM (Dulbecco’s Modified Eagle Medium) containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) at 37 °C in 5% CO 2 .

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Over Expression

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effect of m 6 A Recognition Protein YTHDC1 on Skeletal Muscle Growth

doi: 10.3390/ani15131978

Figure Lengend Snippet: Effect of YTHDC1 on C2C12 cell differentiation assessed by real-time fluorescent quantitative PCR and Western blotting. ( A ) Relative protein expression of interference YTHDC1. ( B ) Relative mRNA expression of overexpression YTHDC1. ( C ) Relative protein expression of interference YTHDC1 ( D ) Relative protein expression of overexpression YTHDC1. ns, no significance, * p < 0.05; ** p < 0.01.

Article Snippet: Mouse C2C12 myoblast cells were cultured in DMEM (Dulbecco’s Modified Eagle Medium) containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) at 37 °C in 5% CO 2 .

Techniques: Cell Differentiation, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Over Expression

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Cell viability of C2C12 in ATPS systems. a-i) Confocal images with C2C12 labelled with Calcein to show live cells (green) and cells labelled with propidium iodide to show dead cells (red). Quantification of cell viability in ATPS systems where a-ii) GelMA was cross-linked and in samples where a-iii) GelMA was removed. Scale bars: (a) 100 µm. Statistical significances were assessed by two-way ANOVA. Mean ± S.D. n=3, *p<0.05.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques:

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Spatial distribution of C2C12 in ATPS systems. a-b-c) Confocal images with C2C12 labelled with DAPI to show nuclei (blue) and cells marked with phalloidin to show f-actin (magenta). d) Orthogonal view of image c-i. Circularity analysis conducted on the formulation e-i) NaCl 0 g/L with GelMA, e-ii) NaCl 9 g/L with GelMA, e-iii) NaCl 36 g/L with GelMA, f-i) NaCl 0 g/L without GelMA, f-ii) NaCl 9 g/L without GelMA and f-iii) NaCl 36 g/L without GelMA. g) Cell circularity as a function of salt concentration. g-i) Cell circularity for each NaCl composition within the ATPS samples is shown. The pink contour marks samples with + GelMA; the blue contour marks samples with - GelMA. g-ii) Cell circularity is inversely proportional to the salt concentration in the hydrogels. When the amount of NaCl in the scaffolds is higher, the cells are more elongated. Scale bars: (c) 100 µm. Statistical significances were assessed by two-way ANOVA. Mean ± S.D. n=3, ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques: Formulation, Concentration Assay

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Partitioning of different cell types within the various ATPS scaffold formulations. Brightfield and confocal (merged) images representative of cells labelled with DAPI to show the nucleus (blue) of the different cell types (A549, C2C12, MG63, HBMSCs), and GelMA marked with rhodamine B to show the inner phase (red) of the scaffolds at 0 - 9 - 36 g/L. In all cell types, images were taken both under conditions where GelMA was not chemically cross-linked (-GelMA) and under conditions where GelMA was chemically cross-linked (+GelMA). Scale bars: (a, b, c, d) 100 μm. Mean ± S.D. n=3.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques:

Journal: Molecular Medicine Reports

Article Title: Glucose, glutamine, lactic acid and α-ketoglutarate restore metabolic disturbances and atrophic changes in energy-deprived muscle cells

doi: 10.3892/mmr.2025.13562

Figure Lengend Snippet: Effect of single nutrient supplementation on metabolic activity. (A) Cells were incubated in regular DMEM supplemented with the indicated nutrient for 24 h. (B and C) C2C12 myotubes were cultured in starvation medium only or with the indicated nutrient for (B) 5 or (C) 24 h. Albumin and NaOH were added as vehicle controls in both regular and starvation media in the experiment for the fatty acid (PA and OA). Metabolic activity was assessed using the MTT assay. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as the mean ± SEM (n=3-4). ***P<0.001 vs. vehicle control; § P<0.05, §§ P<0.01, §§§ P<0.001 vs. starved cells in the same group. Cont, control; Vehicle, vehicle control; Stv, starvation; Glc, glucose; Gln, glutamine; Glu, glutamic acid; Leu, leucine; Val, valine; LA, lactate; βOHB, β-hydroxy butyric acid; αKG, α-ketoglutarate; PA, palmitic acid; OA, oleic acid.

Article Snippet: Mouse C2C12 myoblast cell line (RCB0987) was purchased from the RIKEN BioResource Research Center (Tsukuba, Japan).

Techniques: Activity Assay, Incubation, Cell Culture, MTT Assay, Control

Journal: Molecular Medicine Reports

Article Title: Glucose, glutamine, lactic acid and α-ketoglutarate restore metabolic disturbances and atrophic changes in energy-deprived muscle cells

doi: 10.3892/mmr.2025.13562

Figure Lengend Snippet: Effect of single nutrient supplementation on ATP production. C2C12 myotubes were incubated in starvation medium with or without the indicated nutrients for 24 h. The ATP production rates from glycolysis (Glyco-ATP) and OxPhos (OxPhos-ATP) were determined. Values are expressed as fold-change compared with the values of the control cells incubated in regular DMEM. Values are presented as mean ± SEM (n=3-4). **P<0.01, ***P<0.001 vs. control cells for total ATP production; §§§ P<0.001 vs. starved cells for Glyco-ATP production. Con, control; Glyco-ATP, glycolytic ATP; OxPhos, oxidative phosphorylation; Stv, starvation.

Article Snippet: Mouse C2C12 myoblast cell line (RCB0987) was purchased from the RIKEN BioResource Research Center (Tsukuba, Japan).

Techniques: Incubation, Control, Phospho-proteomics