myf5  (Thermo Fisher)


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    Name:
    MYF5, Polyclonal
    Description:

    Catalog Number:
    PA518927
    Price:
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    Score:
    85
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    Name:
    LIPG, Polyclonal
    Description:

    Catalog Number:
    PA519032
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    85
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    Structured Review

    Thermo Fisher myf5
    Differentiation of myoblasts into myotubes is inhibited by SVF cells and HGF. a Immunofluorescence staining of fast type myosin heavy chain (green) in C2C12 mouse myoblasts after 4 days in growth medium (GM), differentiation medium (DM), indirect co-culture with SVF cells in DM (SVF), and DM supplemented with 10 ng/ml HGF (HGF). Cells were counterstained with DAPI (blue). Bar = 200 μm. The expression of myogenic regulatory factor 5 ( <t>Myf5</t> ), slow type myosin heavy chain ( Myhc1 ), and fast type myosin heavy chain ( Myhc2 ) mRNA in C2C12 myoblasts after b 7 days or c 14 days of differentiation in the various culture conditions. Western blot of fast type myosin heavy chain (MyHC2) protein is also shown. β-actin was used as a loading control. Statistical analysis was performed using one-way ANOVA with post hoc test (Bonferroni correction). Results are presented as mean ± standard deviation. n = 3. * P

    https://www.bioz.com/result/myf5/product/Thermo Fisher
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    myf5 - by Bioz Stars, 2019-10
    96/100 stars

    Images

    1) Product Images from "The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts"

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-018-1096-6

    Differentiation of myoblasts into myotubes is inhibited by SVF cells and HGF. a Immunofluorescence staining of fast type myosin heavy chain (green) in C2C12 mouse myoblasts after 4 days in growth medium (GM), differentiation medium (DM), indirect co-culture with SVF cells in DM (SVF), and DM supplemented with 10 ng/ml HGF (HGF). Cells were counterstained with DAPI (blue). Bar = 200 μm. The expression of myogenic regulatory factor 5 ( Myf5 ), slow type myosin heavy chain ( Myhc1 ), and fast type myosin heavy chain ( Myhc2 ) mRNA in C2C12 myoblasts after b 7 days or c 14 days of differentiation in the various culture conditions. Western blot of fast type myosin heavy chain (MyHC2) protein is also shown. β-actin was used as a loading control. Statistical analysis was performed using one-way ANOVA with post hoc test (Bonferroni correction). Results are presented as mean ± standard deviation. n = 3. * P
    Figure Legend Snippet: Differentiation of myoblasts into myotubes is inhibited by SVF cells and HGF. a Immunofluorescence staining of fast type myosin heavy chain (green) in C2C12 mouse myoblasts after 4 days in growth medium (GM), differentiation medium (DM), indirect co-culture with SVF cells in DM (SVF), and DM supplemented with 10 ng/ml HGF (HGF). Cells were counterstained with DAPI (blue). Bar = 200 μm. The expression of myogenic regulatory factor 5 ( Myf5 ), slow type myosin heavy chain ( Myhc1 ), and fast type myosin heavy chain ( Myhc2 ) mRNA in C2C12 myoblasts after b 7 days or c 14 days of differentiation in the various culture conditions. Western blot of fast type myosin heavy chain (MyHC2) protein is also shown. β-actin was used as a loading control. Statistical analysis was performed using one-way ANOVA with post hoc test (Bonferroni correction). Results are presented as mean ± standard deviation. n = 3. * P

    Techniques Used: Immunofluorescence, Staining, Co-Culture Assay, Expressing, Western Blot, Standard Deviation

    2) Product Images from "Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus"

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2018.01705

    The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J ) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers myf5 and myoD is perturbed in fgfr4 CRISPR embryos. (K, L) Xbra expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; ** p
    Figure Legend Snippet: The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J ) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers myf5 and myoD is perturbed in fgfr4 CRISPR embryos. (K, L) Xbra expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; ** p

    Techniques Used: CRISPR, Expressing, Marker

    3) Product Images from ""

    Article Title:

    Journal:

    doi: 10.1074/jbc.M112.376640

    GATA-4 and TAL1 increase myoblast proliferation, change MRF expression patterns in C2C12 myoblasts, and delay myogenic differentiation. A , cell proliferation was determined by BrdU cell uptake in C2C12 myoblasts with Myf5 and MyoD knockdown and TAL1,
    Figure Legend Snippet: GATA-4 and TAL1 increase myoblast proliferation, change MRF expression patterns in C2C12 myoblasts, and delay myogenic differentiation. A , cell proliferation was determined by BrdU cell uptake in C2C12 myoblasts with Myf5 and MyoD knockdown and TAL1,

    Techniques Used: Expressing

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Article Snippet: Paragraph title: Gene expression analysis using real-time quantitative PCR ... Probes used for evaluating the degree of differentiation in myoblasts included: MYF5 (Applied Biosystems; #Rn01502778; #Mm00435125), MYH1 (Applied Biosystems; #Rn01751056; Mm01332489), and MYH2 (Applied Biosystems; #Rn01470656; Mm01332564).

    Article Title:
    Article Snippet: Paragraph title: Overexpression, RNAi, and Quantitative Real-time PCR ... GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells.

    Transfection:

    Article Title:
    Article Snippet: GATA-4, TAL1, and PGC-1α cDNA expression vectors were transfected into C2C12 cells using the transfection reagent LipofectamineTM 2000 (Invitrogen). .. GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells. .. For differentiated cells, C2C12 cells were transfected with siRNA in growth medium and after 24 h the cultures were changed to differentiation medium and cultured for an additional required time prior to harvesting.

    Amplification:

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Article Snippet: The amplification was performed on a ViiA 7 Real-time PCR system (Applied Biosystems). .. Probes used for evaluating the degree of differentiation in myoblasts included: MYF5 (Applied Biosystems; #Rn01502778; #Mm00435125), MYH1 (Applied Biosystems; #Rn01751056; Mm01332489), and MYH2 (Applied Biosystems; #Rn01470656; Mm01332564).

    In Vitro:

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: D-loops were defined as outflow tracts directed to the right, and L-loops to the left. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Embryos were collected at the desired stages, fixed in MEMFA for 1–2 h at room temperature (RT) and dehydrated in 100% ethanol.

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: D-loops were defined as outflow tracts directed to the right, and L-loops to the left. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Embryos were collected at the desired stages, fixed in MEMFA for 1–2 h at room temperature (RT) and dehydrated in 100% ethanol.

    Synthesized:

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Article Snippet: Total RNA was extracted from cells using the RNeasy Mini Kit (Qiagen; #74106). cDNA was synthesized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; #4268813), and qRT-PCR was performed with TaqMan gene expression assay (Applied Biosystems). .. Probes used for evaluating the degree of differentiation in myoblasts included: MYF5 (Applied Biosystems; #Rn01502778; #Mm00435125), MYH1 (Applied Biosystems; #Rn01751056; Mm01332489), and MYH2 (Applied Biosystems; #Rn01470656; Mm01332564).

    Negative Control:

    Article Title:
    Article Snippet: GATA-4, TAL1, and PGC-1α cDNA expression vectors were transfected into C2C12 cells using the transfection reagent LipofectamineTM 2000 (Invitrogen). .. GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells. .. For differentiated cells, C2C12 cells were transfected with siRNA in growth medium and after 24 h the cultures were changed to differentiation medium and cultured for an additional required time prior to harvesting.

    Quantitative RT-PCR:

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Article Snippet: Total RNA was extracted from cells using the RNeasy Mini Kit (Qiagen; #74106). cDNA was synthesized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; #4268813), and qRT-PCR was performed with TaqMan gene expression assay (Applied Biosystems). .. Probes used for evaluating the degree of differentiation in myoblasts included: MYF5 (Applied Biosystems; #Rn01502778; #Mm00435125), MYH1 (Applied Biosystems; #Rn01751056; Mm01332489), and MYH2 (Applied Biosystems; #Rn01470656; Mm01332564).

    Pyrolysis Gas Chromatography:

    Article Title:
    Article Snippet: GATA-4, TAL1, and PGC-1α cDNA expression vectors were transfected into C2C12 cells using the transfection reagent LipofectamineTM 2000 (Invitrogen). .. GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells.

    SYBR Green Assay:

    Article Title:
    Article Snippet: GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells. .. For differentiated cells, C2C12 cells were transfected with siRNA in growth medium and after 24 h the cultures were changed to differentiation medium and cultured for an additional required time prior to harvesting.

    Polymerase Chain Reaction:

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Article Snippet: Expression of 84 growth factors in SVF cells and dASCs was analyzed using the Rat Growth Factors RT2 PCR Array (Qiagen; #PARN-041ZC). .. Probes used for evaluating the degree of differentiation in myoblasts included: MYF5 (Applied Biosystems; #Rn01502778; #Mm00435125), MYH1 (Applied Biosystems; #Rn01751056; Mm01332489), and MYH2 (Applied Biosystems; #Rn01470656; Mm01332564).

    Incubation:

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

    Labeling:

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

    Article Title:
    Article Snippet: GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells. .. For differentiated cells, C2C12 cells were transfected with siRNA in growth medium and after 24 h the cultures were changed to differentiation medium and cultured for an additional required time prior to harvesting.

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

    Expressing:

    Article Title: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Article Snippet: Paragraph title: Gene expression analysis using real-time quantitative PCR ... Probes used for evaluating the degree of differentiation in myoblasts included: MYF5 (Applied Biosystems; #Rn01502778; #Mm00435125), MYH1 (Applied Biosystems; #Rn01751056; Mm01332489), and MYH2 (Applied Biosystems; #Rn01470656; Mm01332564).

    Article Title:
    Article Snippet: GATA-4, TAL1, and PGC-1α cDNA expression vectors were transfected into C2C12 cells using the transfection reagent LipofectamineTM 2000 (Invitrogen). .. GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells.

    Sequencing:

    Article Title:
    Article Snippet: GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells. .. For differentiated cells, C2C12 cells were transfected with siRNA in growth medium and after 24 h the cultures were changed to differentiation medium and cultured for an additional required time prior to harvesting.

    Over Expression:

    Article Title:
    Article Snippet: Paragraph title: Overexpression, RNAi, and Quantitative Real-time PCR ... GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells.

    In Situ Hybridization:

    Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
    Article Snippet: Paragraph title: In situ Hybridization ... Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

    Plasmid Preparation:

    Article Title:
    Article Snippet: GATA-4, TAL1, Sirt1, Myf5, MyoD, EpoR, and negative control siRNAs (Thermo Scientific Dharmacon) were transfected into C2C12 cells. .. The quantitative real-time PCR assay was carried out using gene-specific primers (available upon request) and fluorescently labeled TaqMan probes or SYBR Green dye (Invitrogen) in a 7900 Sequence Detector (PE Applied Biosystems, Foster City, CA).

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    Thermo Fisher gene exp myf5 hs00271574 m1
    SNAIL is a direct transcriptional repressor of <t>MYF5</t> expression and regulator of MYOD activity. a SNAIL silencing in RH30 cells induces MYOD binding to GC rich E-Box sequences. Nuclear protein extracts from both undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium supplemented with 2% HS were used in TransAM MyoD DNA-binding ELISA. To monitor the specificity of the assay, the wild-type consensus oligonucleotide (wt) and mutated (mut) sequences were used as competitors for MYOD binding from cell extracts; n = 3. b SNAIL binds to the MYF5 and E-cadherin promoters in RH30 cells. The promoter of MYF5 (~1000 bb) was screened for putative SNAIL transcription factor binding sites and the results were validated by Chip Assay. The images depict one representative result of the ChIP assay. Proteins bound to DNA were immunoprecipitated with the anti-SNAIL antibody, negative IgG control, positive histone H3 control and input DNA control was analyzed. Fragments of the MYF5 and E-cadherin promoters were amplified by PCR and visualized on agarose gels stained with ethidium bromide. c SNAIL silencing by siRNA in RH30 WT cells induces MYF5 promoter activation and luciferase expression when cells are transfected with MYF5@pNL plasmid (luciferase under control with MYF5 promoter), whereas MYOD silencing by siRNA does not exert any effect. SNAIL and MYOD compete for binding to MYF5 promoter in RH30 shSNAIL cells ( d ), RH41 WT ( e ), and RH41 shSNAIL cells ( f ), when cells are transfected with MYF5@pNL plasmid and siRNA against SNAIL, MYOD or siSCR (scrambled siRNA). The data were normalized to mCherry fluorescence level in each well. pNL (luciferase plasmid without promoter) served as a negative control and Ubc@pNL plasmid (luciferase under control of ubiquitin C promoter) was a positive control. The data represent the mean ± SEM or are representative images of at least 3 independent experiments. * p
    Gene Exp Myf5 Hs00271574 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp myf5 hs00271574 m1/product/Thermo Fisher
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    gene exp myf5 hs00271574 m1 - by Bioz Stars, 2019-10
    89/100 stars
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    95
    Thermo Fisher gene exp myf5 mm00435125 m1
    Syndecan-3 regulates myoblast homeostasis and migration. a Wild type (WT, top panels ) and Sdc3 −/− (S3 −/− , bottom panels ) myofibers cultured in suspension for 4 days, fixed, and immunostained to detect Sdc4 ( white ), Pax7 ( red ), <t>Myf5</t> ( green ), and nuclei (blue). Arrows indicate a satellite cell doublet on a Sdc3 −/− myofiber where one cell is Myf5 + Pax7+ and the other cell is Myf5 + Pax7−. b , c Quantification of Myf5 + Pax7− cells ( b ) and Myf5 + Pax7+ cells ( c ) as in a . d Muscle cross sections identifying Myf5+ cells ( green ) in Sdc3 −/− (S3 −/− ) and wild type (WT) muscles 3 months post-injury. Arrows are interstitial cells; arrowheads are sublaminar cells. e , f Quantification of sublaminar ( e ) and interstitial ( f ) Myf5+ cells (normalized to area) in Sdc3 −/− (S3 −/− ) and wild type (WT) muscles 3 months post-injury. g Quantification of the numbers of Ki67+ cells (normalized to area) in wild type (WT) and Sdc3 −/− (S3 −/− ) muscles 3 months post-injury. h More myoblasts migrate away from Sdc3 −/− (S3 −/− ) myofibers transferred to gelatin-coated coverslips after 2.5 days culture in suspension than from wild type (WT) myofibers. i Quantification of adherent myoblasts 4 h after myofiber transfer as in g . Scale bars are 100 μm in a , 50 μm in e , and 20 μm in f . Error bars are S.E.M. and ** = p
    Gene Exp Myf5 Mm00435125 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp myf5 mm00435125 m1/product/Thermo Fisher
    Average 95 stars, based on 7 article reviews
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    92
    Thermo Fisher gene exp myf5 hs00929416 g1
    mRNA expression of a MyoD, b <t>Myf5,</t> c MyoG, d Myf6 and e myostatin before and 96 h after a single bout of resistance exercise (RE) versus resistance exercise and high-intensity interval training (RE + HIIT). Data presented as mean ± SEM
    Gene Exp Myf5 Hs00929416 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp myf5 hs00929416 g1/product/Thermo Fisher
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    gene exp myf5 hs00929416 g1 - by Bioz Stars, 2019-10
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    Image Search Results


    SNAIL is a direct transcriptional repressor of MYF5 expression and regulator of MYOD activity. a SNAIL silencing in RH30 cells induces MYOD binding to GC rich E-Box sequences. Nuclear protein extracts from both undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium supplemented with 2% HS were used in TransAM MyoD DNA-binding ELISA. To monitor the specificity of the assay, the wild-type consensus oligonucleotide (wt) and mutated (mut) sequences were used as competitors for MYOD binding from cell extracts; n = 3. b SNAIL binds to the MYF5 and E-cadherin promoters in RH30 cells. The promoter of MYF5 (~1000 bb) was screened for putative SNAIL transcription factor binding sites and the results were validated by Chip Assay. The images depict one representative result of the ChIP assay. Proteins bound to DNA were immunoprecipitated with the anti-SNAIL antibody, negative IgG control, positive histone H3 control and input DNA control was analyzed. Fragments of the MYF5 and E-cadherin promoters were amplified by PCR and visualized on agarose gels stained with ethidium bromide. c SNAIL silencing by siRNA in RH30 WT cells induces MYF5 promoter activation and luciferase expression when cells are transfected with MYF5@pNL plasmid (luciferase under control with MYF5 promoter), whereas MYOD silencing by siRNA does not exert any effect. SNAIL and MYOD compete for binding to MYF5 promoter in RH30 shSNAIL cells ( d ), RH41 WT ( e ), and RH41 shSNAIL cells ( f ), when cells are transfected with MYF5@pNL plasmid and siRNA against SNAIL, MYOD or siSCR (scrambled siRNA). The data were normalized to mCherry fluorescence level in each well. pNL (luciferase plasmid without promoter) served as a negative control and Ubc@pNL plasmid (luciferase under control of ubiquitin C promoter) was a positive control. The data represent the mean ± SEM or are representative images of at least 3 independent experiments. * p

    Journal: Cell Death & Disease

    Article Title: SNAIL is a key regulator of alveolar rhabdomyosarcoma tumor growth and differentiation through repression of MYF5 and MYOD function

    doi: 10.1038/s41419-018-0693-8

    Figure Lengend Snippet: SNAIL is a direct transcriptional repressor of MYF5 expression and regulator of MYOD activity. a SNAIL silencing in RH30 cells induces MYOD binding to GC rich E-Box sequences. Nuclear protein extracts from both undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium supplemented with 2% HS were used in TransAM MyoD DNA-binding ELISA. To monitor the specificity of the assay, the wild-type consensus oligonucleotide (wt) and mutated (mut) sequences were used as competitors for MYOD binding from cell extracts; n = 3. b SNAIL binds to the MYF5 and E-cadherin promoters in RH30 cells. The promoter of MYF5 (~1000 bb) was screened for putative SNAIL transcription factor binding sites and the results were validated by Chip Assay. The images depict one representative result of the ChIP assay. Proteins bound to DNA were immunoprecipitated with the anti-SNAIL antibody, negative IgG control, positive histone H3 control and input DNA control was analyzed. Fragments of the MYF5 and E-cadherin promoters were amplified by PCR and visualized on agarose gels stained with ethidium bromide. c SNAIL silencing by siRNA in RH30 WT cells induces MYF5 promoter activation and luciferase expression when cells are transfected with MYF5@pNL plasmid (luciferase under control with MYF5 promoter), whereas MYOD silencing by siRNA does not exert any effect. SNAIL and MYOD compete for binding to MYF5 promoter in RH30 shSNAIL cells ( d ), RH41 WT ( e ), and RH41 shSNAIL cells ( f ), when cells are transfected with MYF5@pNL plasmid and siRNA against SNAIL, MYOD or siSCR (scrambled siRNA). The data were normalized to mCherry fluorescence level in each well. pNL (luciferase plasmid without promoter) served as a negative control and Ubc@pNL plasmid (luciferase under control of ubiquitin C promoter) was a positive control. The data represent the mean ± SEM or are representative images of at least 3 independent experiments. * p

    Article Snippet: Gene expression was determined by qRT-PCR analysis using ABI PRISM 7300 Sequence Detection System or Quant Studio 7 Flex System (both from Applied Biosystems, Foster City, CA, USA), Blank qPCR Master Mix (EURx) and the indicated Taq-Man probes (Applied Biosystems): human: GAPDH (Hs99999905_m1), MYF5 (Hs00271574_m1), MYOD (Hs00159528_m1), MRF4 (Hs01547104_g1), MEF2A (Hs01050409_m1), MYOSTATIN (Hs00976237_m1), MYOGENIN (Hs01032275_m1), MYH2 (Hs00430042_m1), SNAI1 (Hs00195591_m1), HDAC1 (Hs02621185_s1) and HDAC2 (Hs00231032_m1).

    Techniques: Expressing, Activity Assay, Binding Assay, Gas Chromatography, Enzyme-linked Immunosorbent Assay, Chromatin Immunoprecipitation, Immunoprecipitation, Amplification, Polymerase Chain Reaction, Staining, Activation Assay, Luciferase, Transfection, Plasmid Preparation, Fluorescence, Negative Control, Positive Control

    SNAIL silencing in human myoblasts increases expression of myogenic factors. a SNAIL expression in human myoblasts diminishes with subsequent passages (qPCR, n = 4). b Human myoblasts were transfected with siRNA against SNAIL (siSNAIL) and with a scrambled siRNA sequence (siRNA) or they were treated with transfection reagent (mock). 24 h after transfection they were treated with a differentiating medium containing 2% HS for the next 48 h. SNAIL silencing was validated by Western blot. SNAIL silencing increased the myogenin and MyHC protein levels (Western blot). c Expression of SNAIL, MYF5, MYOD, MRF4, myogenin and MyHC was determined by qPCR with ΔCT quantification method and GAPDH as a housekeeping gene control. SNAIL silencing and differentiation induces expression of the analyzed myogenic factors. d SNAIL silencing increases the number of the myotubes with high MyHC expression and induces their fusion. The images are representative merged images of immunofluorescent staining for MyHC (MyHC: red; nuclei: Hoechst, blue) of myotubes. The scale bar represents 100 μm. Fusion index was calculated by expressing the number of nuclei within MyHC-positive myotubes with ≥2 nuclei as a percentage of the total nuclei; n = 4. e Mechanism of action of SNAIL transcription factor on myogenic differentiation of ARMS. Data on graphs are represented as mean ± SEM. * p

    Journal: Cell Death & Disease

    Article Title: SNAIL is a key regulator of alveolar rhabdomyosarcoma tumor growth and differentiation through repression of MYF5 and MYOD function

    doi: 10.1038/s41419-018-0693-8

    Figure Lengend Snippet: SNAIL silencing in human myoblasts increases expression of myogenic factors. a SNAIL expression in human myoblasts diminishes with subsequent passages (qPCR, n = 4). b Human myoblasts were transfected with siRNA against SNAIL (siSNAIL) and with a scrambled siRNA sequence (siRNA) or they were treated with transfection reagent (mock). 24 h after transfection they were treated with a differentiating medium containing 2% HS for the next 48 h. SNAIL silencing was validated by Western blot. SNAIL silencing increased the myogenin and MyHC protein levels (Western blot). c Expression of SNAIL, MYF5, MYOD, MRF4, myogenin and MyHC was determined by qPCR with ΔCT quantification method and GAPDH as a housekeeping gene control. SNAIL silencing and differentiation induces expression of the analyzed myogenic factors. d SNAIL silencing increases the number of the myotubes with high MyHC expression and induces their fusion. The images are representative merged images of immunofluorescent staining for MyHC (MyHC: red; nuclei: Hoechst, blue) of myotubes. The scale bar represents 100 μm. Fusion index was calculated by expressing the number of nuclei within MyHC-positive myotubes with ≥2 nuclei as a percentage of the total nuclei; n = 4. e Mechanism of action of SNAIL transcription factor on myogenic differentiation of ARMS. Data on graphs are represented as mean ± SEM. * p

    Article Snippet: Gene expression was determined by qRT-PCR analysis using ABI PRISM 7300 Sequence Detection System or Quant Studio 7 Flex System (both from Applied Biosystems, Foster City, CA, USA), Blank qPCR Master Mix (EURx) and the indicated Taq-Man probes (Applied Biosystems): human: GAPDH (Hs99999905_m1), MYF5 (Hs00271574_m1), MYOD (Hs00159528_m1), MRF4 (Hs01547104_g1), MEF2A (Hs01050409_m1), MYOSTATIN (Hs00976237_m1), MYOGENIN (Hs01032275_m1), MYH2 (Hs00430042_m1), SNAI1 (Hs00195591_m1), HDAC1 (Hs02621185_s1) and HDAC2 (Hs00231032_m1).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Sequencing, Western Blot, Staining

    SNAIL expression is associated with RMS subtype and myogenic differentiation. a Expression of early and late myogenic factors in 158 RMS samples was estimated previously by microarray and deposited in GEO database with accession number: GSE92689 (ref. 20 ). SNAIL level negatively correlates with MYF5 level in RMS (Pearson correlation). b SNAIL level positively correlates with MYOD level in RMS (Pearson correlation). R—Pearson correlation coefficient; p—significance value ( c ) Increased SNAIL levels in RMS stage 2,3 and 4 compared to 1 were demonstrated in a group of 158 RMS samples (microarray data, GEO database GSE92689). The data are presented as Whisker plots min to max. d Increased SNAIL level in ARMS compared to ERMS was demonstrated in a group of 158 RMS samples (microarray data, GEO database GSE92689). The data are presented as Whisker plots min to max. e SNAIL expression was quantified by qPCR using the ΔCt quantification method and GAPDH as a housekeeping gene control in ARMS and ERMS cell lines and human primary myoblasts. The SNAIL level is increased in RH30 and RH41 ARMS cell lines compared to RD and RH18 ERMS cell lines, and the level is comparable with the SNAIL level in myoblasts at the early passage (undiff.), whereas in the differentiated myotubes (diff.) SNAIL level is more comparable with ERMS; n = 3. f Differentiation of RH30 cells by culture in medium supplemented with 2% HS for 7 days downregulates SNAIL expression at the mRNA level (qPCR) and protein level (Western blotting); n = 4. The data represent the mean ± SEM. * p

    Journal: Cell Death & Disease

    Article Title: SNAIL is a key regulator of alveolar rhabdomyosarcoma tumor growth and differentiation through repression of MYF5 and MYOD function

    doi: 10.1038/s41419-018-0693-8

    Figure Lengend Snippet: SNAIL expression is associated with RMS subtype and myogenic differentiation. a Expression of early and late myogenic factors in 158 RMS samples was estimated previously by microarray and deposited in GEO database with accession number: GSE92689 (ref. 20 ). SNAIL level negatively correlates with MYF5 level in RMS (Pearson correlation). b SNAIL level positively correlates with MYOD level in RMS (Pearson correlation). R—Pearson correlation coefficient; p—significance value ( c ) Increased SNAIL levels in RMS stage 2,3 and 4 compared to 1 were demonstrated in a group of 158 RMS samples (microarray data, GEO database GSE92689). The data are presented as Whisker plots min to max. d Increased SNAIL level in ARMS compared to ERMS was demonstrated in a group of 158 RMS samples (microarray data, GEO database GSE92689). The data are presented as Whisker plots min to max. e SNAIL expression was quantified by qPCR using the ΔCt quantification method and GAPDH as a housekeeping gene control in ARMS and ERMS cell lines and human primary myoblasts. The SNAIL level is increased in RH30 and RH41 ARMS cell lines compared to RD and RH18 ERMS cell lines, and the level is comparable with the SNAIL level in myoblasts at the early passage (undiff.), whereas in the differentiated myotubes (diff.) SNAIL level is more comparable with ERMS; n = 3. f Differentiation of RH30 cells by culture in medium supplemented with 2% HS for 7 days downregulates SNAIL expression at the mRNA level (qPCR) and protein level (Western blotting); n = 4. The data represent the mean ± SEM. * p

    Article Snippet: Gene expression was determined by qRT-PCR analysis using ABI PRISM 7300 Sequence Detection System or Quant Studio 7 Flex System (both from Applied Biosystems, Foster City, CA, USA), Blank qPCR Master Mix (EURx) and the indicated Taq-Man probes (Applied Biosystems): human: GAPDH (Hs99999905_m1), MYF5 (Hs00271574_m1), MYOD (Hs00159528_m1), MRF4 (Hs01547104_g1), MEF2A (Hs01050409_m1), MYOSTATIN (Hs00976237_m1), MYOGENIN (Hs01032275_m1), MYH2 (Hs00430042_m1), SNAI1 (Hs00195591_m1), HDAC1 (Hs02621185_s1) and HDAC2 (Hs00231032_m1).

    Techniques: Expressing, Microarray, Whisker Assay, Real-time Polymerase Chain Reaction, Western Blot

    SNAIL silencing induces MYF5 expression in ARMS cells. a SNAIL silencing does not significantly affect MYOD expression at the mRNA (qPCR, n = 3) or protein level (Western blot) in undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium with 2% HS. b MYF5 is expressed in the nuclei of RH30 shSNAIL cells. The MYF5 protein level was visualized by Western blot and by immunofluorescent staining (red color), and the nuclei were visualized with DAPI (blue). The results are presented as merged images. The white scale bar represents 10 μm. c SNAIL silencing induces the expression of MYF5 mRNA in both undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium with 2% HS (qPCR, n = 3) d SNAIL silencing slightly induces the expression of MYF5 mRNA in both undifferentiated RH41 cells and RH41 cells differentiated for 7 days in medium with 2% HS (qPCR, n = 3). e Transient SNAIL silencing with siRNA in RH30 and RH41 ARMS cell lines slightly induces MYF5 expression. The cells were transfected with siRNA against SNAIL (siSNAIL) and a scrambled siRNA sequence (siRNA). Twenty-four hours after transfection, the cells were treated with differentiating medium containing 2% HS for the following 48 h. MYF5 levels were validated by qPCR; n = 3. f Transfection with pre-miR-30a-5p in RH30 cells induces MYF5 expression by downregulation of SNAIL protein. RH30 cells were transfected with the miRNA precursor pre-miR-30a-5p and pre-miR negative control (miR-neg-ctrl). Twenty-four hours after transfection, the cells were treated with differentiating medium containing 2% HS for the following 48 h. miR-30a-5p expression relative to U6 snRNA and MYF5, MYOD expression relative to GAPDH were validated by qPCR; n = 4. SNAIL protein level was validated by Western blot. The data represent the mean ± SEM. * p

    Journal: Cell Death & Disease

    Article Title: SNAIL is a key regulator of alveolar rhabdomyosarcoma tumor growth and differentiation through repression of MYF5 and MYOD function

    doi: 10.1038/s41419-018-0693-8

    Figure Lengend Snippet: SNAIL silencing induces MYF5 expression in ARMS cells. a SNAIL silencing does not significantly affect MYOD expression at the mRNA (qPCR, n = 3) or protein level (Western blot) in undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium with 2% HS. b MYF5 is expressed in the nuclei of RH30 shSNAIL cells. The MYF5 protein level was visualized by Western blot and by immunofluorescent staining (red color), and the nuclei were visualized with DAPI (blue). The results are presented as merged images. The white scale bar represents 10 μm. c SNAIL silencing induces the expression of MYF5 mRNA in both undifferentiated RH30 cells and RH30 cells differentiated for 7 days in medium with 2% HS (qPCR, n = 3) d SNAIL silencing slightly induces the expression of MYF5 mRNA in both undifferentiated RH41 cells and RH41 cells differentiated for 7 days in medium with 2% HS (qPCR, n = 3). e Transient SNAIL silencing with siRNA in RH30 and RH41 ARMS cell lines slightly induces MYF5 expression. The cells were transfected with siRNA against SNAIL (siSNAIL) and a scrambled siRNA sequence (siRNA). Twenty-four hours after transfection, the cells were treated with differentiating medium containing 2% HS for the following 48 h. MYF5 levels were validated by qPCR; n = 3. f Transfection with pre-miR-30a-5p in RH30 cells induces MYF5 expression by downregulation of SNAIL protein. RH30 cells were transfected with the miRNA precursor pre-miR-30a-5p and pre-miR negative control (miR-neg-ctrl). Twenty-four hours after transfection, the cells were treated with differentiating medium containing 2% HS for the following 48 h. miR-30a-5p expression relative to U6 snRNA and MYF5, MYOD expression relative to GAPDH were validated by qPCR; n = 4. SNAIL protein level was validated by Western blot. The data represent the mean ± SEM. * p

    Article Snippet: Gene expression was determined by qRT-PCR analysis using ABI PRISM 7300 Sequence Detection System or Quant Studio 7 Flex System (both from Applied Biosystems, Foster City, CA, USA), Blank qPCR Master Mix (EURx) and the indicated Taq-Man probes (Applied Biosystems): human: GAPDH (Hs99999905_m1), MYF5 (Hs00271574_m1), MYOD (Hs00159528_m1), MRF4 (Hs01547104_g1), MEF2A (Hs01050409_m1), MYOSTATIN (Hs00976237_m1), MYOGENIN (Hs01032275_m1), MYH2 (Hs00430042_m1), SNAI1 (Hs00195591_m1), HDAC1 (Hs02621185_s1) and HDAC2 (Hs00231032_m1).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Staining, Transfection, Sequencing, Negative Control

    Syndecan-3 regulates myoblast homeostasis and migration. a Wild type (WT, top panels ) and Sdc3 −/− (S3 −/− , bottom panels ) myofibers cultured in suspension for 4 days, fixed, and immunostained to detect Sdc4 ( white ), Pax7 ( red ), Myf5 ( green ), and nuclei (blue). Arrows indicate a satellite cell doublet on a Sdc3 −/− myofiber where one cell is Myf5 + Pax7+ and the other cell is Myf5 + Pax7−. b , c Quantification of Myf5 + Pax7− cells ( b ) and Myf5 + Pax7+ cells ( c ) as in a . d Muscle cross sections identifying Myf5+ cells ( green ) in Sdc3 −/− (S3 −/− ) and wild type (WT) muscles 3 months post-injury. Arrows are interstitial cells; arrowheads are sublaminar cells. e , f Quantification of sublaminar ( e ) and interstitial ( f ) Myf5+ cells (normalized to area) in Sdc3 −/− (S3 −/− ) and wild type (WT) muscles 3 months post-injury. g Quantification of the numbers of Ki67+ cells (normalized to area) in wild type (WT) and Sdc3 −/− (S3 −/− ) muscles 3 months post-injury. h More myoblasts migrate away from Sdc3 −/− (S3 −/− ) myofibers transferred to gelatin-coated coverslips after 2.5 days culture in suspension than from wild type (WT) myofibers. i Quantification of adherent myoblasts 4 h after myofiber transfer as in g . Scale bars are 100 μm in a , 50 μm in e , and 20 μm in f . Error bars are S.E.M. and ** = p

    Journal: Skeletal Muscle

    Article Title: Loss of niche-satellite cell interactions in syndecan-3 null mice alters muscle progenitor cell homeostasis improving muscle regeneration

    doi: 10.1186/s13395-016-0104-8

    Figure Lengend Snippet: Syndecan-3 regulates myoblast homeostasis and migration. a Wild type (WT, top panels ) and Sdc3 −/− (S3 −/− , bottom panels ) myofibers cultured in suspension for 4 days, fixed, and immunostained to detect Sdc4 ( white ), Pax7 ( red ), Myf5 ( green ), and nuclei (blue). Arrows indicate a satellite cell doublet on a Sdc3 −/− myofiber where one cell is Myf5 + Pax7+ and the other cell is Myf5 + Pax7−. b , c Quantification of Myf5 + Pax7− cells ( b ) and Myf5 + Pax7+ cells ( c ) as in a . d Muscle cross sections identifying Myf5+ cells ( green ) in Sdc3 −/− (S3 −/− ) and wild type (WT) muscles 3 months post-injury. Arrows are interstitial cells; arrowheads are sublaminar cells. e , f Quantification of sublaminar ( e ) and interstitial ( f ) Myf5+ cells (normalized to area) in Sdc3 −/− (S3 −/− ) and wild type (WT) muscles 3 months post-injury. g Quantification of the numbers of Ki67+ cells (normalized to area) in wild type (WT) and Sdc3 −/− (S3 −/− ) muscles 3 months post-injury. h More myoblasts migrate away from Sdc3 −/− (S3 −/− ) myofibers transferred to gelatin-coated coverslips after 2.5 days culture in suspension than from wild type (WT) myofibers. i Quantification of adherent myoblasts 4 h after myofiber transfer as in g . Scale bars are 100 μm in a , 50 μm in e , and 20 μm in f . Error bars are S.E.M. and ** = p

    Article Snippet: Following RNA isolation (CellsDirect Resuspension & Lysis Buffer, Life Technologies) and reverse transcription (High Capacity cDNA Reverse Transcription Kit, Life Technologies) according to the manufacturer’s instructions, complementary DNA (cDNA) was diluted five times in TE buffer and 5 μL were used in a reaction mix containing Droplet Digital™ PCR Supermix (BioRad), 1× TaqMan probes from Life Technologies [Pax7 (Mm03053796-s1), Myf5 (Mm00435125-m1), Hprt (Mm00446968_m1), Pax3 (Mm00435493_m1), and Myod1 (Mm00440387_m1)] and H2 O. Droplets were generated with a QX100 droplet generator (BioRad), after mixing 20 μL of reaction mix and 70 μL of droplet generator oil (BioRad).

    Techniques: Migration, Cell Culture

    Effects of Nandrolone (ND) treatment on the expression level of genes involved in the regenerative pathways in Soleus muscle of HU mice. Histograms show quantification of transcript levels performed with real time PCR, for Notch-1 , MyoD , Myf5 , Myogenin , Pax7 genes normalized by the Hprt1 gene, in the 4 experimental groups (Ctrl, HU, HU-V, HU-ND). Each bar represents the mean ± S.E.M. from the number of animals as indicated in the brackets above the bars. Statistical analysis was performed for each muscle type using ANOVA followed by Fisher t-test *vs Ctrl, °vs HU (at least P

    Journal: PLoS ONE

    Article Title: Effects of Nandrolone in the Counteraction of Skeletal Muscle Atrophy in a Mouse Model of Muscle Disuse: Molecular Biology and Functional Evaluation

    doi: 10.1371/journal.pone.0129686

    Figure Lengend Snippet: Effects of Nandrolone (ND) treatment on the expression level of genes involved in the regenerative pathways in Soleus muscle of HU mice. Histograms show quantification of transcript levels performed with real time PCR, for Notch-1 , MyoD , Myf5 , Myogenin , Pax7 genes normalized by the Hprt1 gene, in the 4 experimental groups (Ctrl, HU, HU-V, HU-ND). Each bar represents the mean ± S.E.M. from the number of animals as indicated in the brackets above the bars. Statistical analysis was performed for each muscle type using ANOVA followed by Fisher t-test *vs Ctrl, °vs HU (at least P

    Article Snippet: TaqMan Hydrolysis primer and probe gene expression assays were ordered by life-technologies with the following assay IDs: mammalian target of rapamycin, serine/threonine kinase (encoded by mTOR gene) assay ID: Mm00444968_m1; eukaryotic translation initiation factor 2 alpha kinase 3 (encoded by Eif2ak3 gene) assay ID: Mm00438700_m1; peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, PGC1a (encoded by Ppargc1a gene) assay ID: Mm01208835_m1; microtubule-associated protein 1 light chain 3 beta, LC3-β (encoded by Map1lc3a gene) assay ID: Mm00458725_g1; cathepsin L (encoded by Ctsl gene) assay ID: Mm00515597_m1; F-box protein 32, also called Atrogin1 (encoded by Fbxo32 gene) assay ID: Mm00499523_m1; tripartite motif-containing 63 also called Murf-1 (encoded by Trim63 gene) assay ID: Mm01185221_m1; myosin heavy chain 1 (encoded by Myh7 gene) assay ID: Mm00600555_m1; myosin heavy chain 2a (encoded by Myh2 gene) assay ID: Mm00454982_m1; myosin heavy chain 2b (encoded by Myh4 gene) assay ID: Mm01332541_m1; myosin heavy chain 2x (encoded by Myh1 gene) assay ID: Mm01332489_m1; notch1 (encoded by Notch1 gene) assay ID: Mm00435249_m1; myogenic differentiation 1 (encoded by MyoD gene) assay ID: Mm00440387_m1; myogenic factor 5 (encoded by Myf5 gene) assay ID: Mm00435125_m1; myogenin (encoded by Myog gene) assay ID: Mm00446194_m1; paired box 7 (encoded by Pax-7 gene) assay ID: Mm01354484_m1; hypoxanthine guanine phosphoribosyl transferase (encoded by Hprt1 gene) assay ID: Mm00446968_m1; actin, beta (encoded by Actb gene) assay ID: Mm00607939_s1; beta-2 microglobulin (encoded by B2m gene) assay ID: Mm00437762_m1.

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction

    mRNA expression of a MyoD, b Myf5, c MyoG, d Myf6 and e myostatin before and 96 h after a single bout of resistance exercise (RE) versus resistance exercise and high-intensity interval training (RE + HIIT). Data presented as mean ± SEM

    Journal: European Journal of Applied Physiology

    Article Title: Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

    doi: 10.1007/s00421-017-3721-y

    Figure Lengend Snippet: mRNA expression of a MyoD, b Myf5, c MyoG, d Myf6 and e myostatin before and 96 h after a single bout of resistance exercise (RE) versus resistance exercise and high-intensity interval training (RE + HIIT). Data presented as mean ± SEM

    Article Snippet: PCR reactions with 2 × TaqMan Universal Master Mix II with UNG (Invitrogen) and 20 × TaqMan Gene Expression assays (Invitrogen) according to the manufacturer’s instructions were used to determine mRNA expression levels for myogenic differentiation 1 (MyoD1, Hs00159528_m1), myogenic factor 5 (Myf5, hs00929416_g1), myogenin (MyoG, Hs00231167_m1), myogenic factor 6 (Myf6, Hs01547104_g1), myostatin (Hs00976237_m1) and β-2-microglobulin (β2M, Hs00984230_m1).

    Techniques: Expressing