Journal: International Journal of Molecular Sciences

Article Title: Elevations of Extracellular Vesicles and Inflammatory Biomarkers in Closed Circuit SCUBA Divers

doi: 10.3390/ijms24065969

Figure Lengend Snippet: Activation of neutrophils from all divers in both groups. Data are the mean ± SD ( n = sample number) % of neutrophils (identified in the flow cytometer based on CD66b expression) expressing myeloperoxidase (MPO) and CD18 above a threshold value as an index of cell activation. Pre- and post-day 1 are values from the deep and shallow groups, and those in the third column reflect values from the shallow group, and the statistical analysis compared against the values only takes this group into account (pre- and post-day 1 as repeated measures ANOVA. (* p < 0.001 vs. pre-dive, RM-ANOVA).

Article Snippet: APC-conjugated anti-human CD146 (cat # 340931) was purchased from BioScience (San Diego, CA, USA), AlexaFluor488-conjugated anti-human TMEM119 (cat # FAB103131G) was from R & D Systems (Minneapolis, MN, USA), anti-thrombospondin (TSP)-1 (cat # sc-393504) was from Santa Cruz Biotechnology (Dallas, TX, USA), and FITC-conjugated anti-human myeloperoxidase (MPO, cat # HM1051PE-100) was from Hycult Biotech (Plymouth Meeting, PA, USA).

Techniques: Activation Assay, Flow Cytometry, Expressing