Journal: Communications Biology

Article Title: Butyrophilin-like 2 regulates site-specific adaptations of intestinal γδ intraepithelial lymphocytes

doi: 10.1038/s42003-021-02438-x

Figure Lengend Snippet: Cohoused 15-week-old Btnl2-KO ( n = 11) and WT ( n = 8) littermates were subjected to 3% DSS-induced colitis for 7 days followed by water for 8 days. Control mice ( n = 2–4) received water. a Body weight loss in cohoused Btnl2-KO and WT littermates calculated as the percent difference between the initial and actual body weight on the above days. Error bars represent mean ± SEM. Significance is measured using unpaired t -tests assuming similar SD, * p < 0.05, ** p < 0.005, *** p < 0.0005, significantly different from DSS-treated WT mice. b Colon length of water- and DSS-treated Btnl2-KO and WT mice on day 15. c H&E histological sections and a pathological score of the colon from water- and DSS-treated Btnl2-KO and WT mice. Scale bars are 200 μm (WT/water), 250 μm (Btnl2-KO/water), 500 μm (WT/DSS and Btnl2-KO/DSS). d Myeloperoxidase (MPO) activity in colon homogenates of water- and DSS-treated Btnl2-KO and WT mice. e Levels of pro-inflammatory cytokines in colon homogenates of water and DSS-treated Btnl2-KO and WT mice. f Granzyme A mRNA levels in colon homogenates of water- and DSS-treated Btnl2-KO and WT mice, normalized to β2m. Error bars represent mean ± SEM. Significance is measured using unpaired t-tests assuming similar SD, * p < 0.05. g Btnl1/2/6 mRNA levels in the colon of water- and DSS-treated WT mice, normalized to β2m. Error bars represent mean ± SEM. Significance is measured using one-way ANOVA, * p < 0.05, ** p < 0.005, *** p < 0.0005.

Article Snippet: Myeloperoxidase (MPO) activity was measured using a mouse MPO ELISA kit according to the manufacturer’s instructions (Hycult Biotech).

Techniques: Activity Assay

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Delayed Akt suppression in the lipopolysaccharide-induced acute lung injury promotes resolution that is associated with enhanced effector regulatory T cells

doi: 10.1152/ajplung.00251.2019

Figure Lengend Snippet: Delayed Akt inhibition enhances the resolution of lung injury. A: a depiction of the experimental design. B: representative images of mouse lung sections (hematoxylin and eosin staining) from control (DMSO) and treatment group showing enhanced resolution of lung injury on day 7 with the triciribine (TCBN) treatment starting from day 2. C: scatterplot showing a significant increase in lung injury score (blindly scored by 3 reviewers) on day 7 after LPS administration that was significantly reduced with TCBN treatment compared with DMSO treated control (n = 6). D: scatterplot showing lung wet/dry weight ratio as a measure of lung edema, demonstrating a significant increase in lung edema with LPS injury that was rescued with TCBN treatment (n = 6–10). E: scatterplot showing significantly decreased bronchoalveolar lavage (BAL) protein content in TCBN-treated lungs compared with DMSO-treated control lungs from LPS-injured mice (n = 6). F: graph showing mouse body weight measured over a period of 7 days after LPS injury, with significant improvement (increase) in body weight with TCBN treatment on day 7 (n = 6). G: bar graph showing significant reduction of myeloperoxidase (MPO) activity in TCBN-treated mice lungs compared with control (n = 6). Data are presented as means ± SD. *P < 0.05; #P < 0.01.

Article Snippet: Myeloperoxidase (MPO) activity assay was performed as described previously ( 6 ) using the Mouse MPO ELISA kit from Hycult biotech (cat. no. HK210-02) according to the manufacturer’s protocol.

Techniques: Inhibition, Staining, Activity Assay

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Delayed Akt suppression in the lipopolysaccharide-induced acute lung injury promotes resolution that is associated with enhanced effector regulatory T cells

doi: 10.1152/ajplung.00251.2019

Figure Lengend Snippet: Regulatory T cell (Treg)-specific phosphatase and tensin homolog (PTEN) knockout (PTENTreg KO) mice exhibited severe lung injury and edema as well as the loss of body weight compared with wild-type (WT) mice. A: representative images of mouse lung sections (hematoxylin and eosin staining) from saline-injured WT and PTENTreg KO controls as well as LPS-treated WT and PTENTreg KO group showing impaired resolution of lung injury on day 7 in PTENTreg KO lungs compared with WT. B and C: scatterplots showing blinded analysis of acute lung injury (ALI) score and lung wet/dry weight ratio indicating a significant increase in lung injury and lung edema in LPS-instilled PTENTreg KO mice compared with WT (n = 5–7). D and E: scatterplot showing elevated bronchoalveolar lavage fluid (BAL) total cell counts and BAL total protein content in LPS-instilled WT mouse lungs compared with the saline-instilled control lungs, which was further exacerbated in LPS-instilled PTENTreg KO mice (n = 5–8). F: body weights of mice measured over a period of 7 days after LPS administration showing a significant decrease in body weights of PTENTreg KO mice compared with WT control (n = 7). G: bar graph showing a significant increase in neutrophil myeloperoxidase (MPO) activity in LPS-instilled PTENTreg KO mouse lungs compared with respective WT control lungs (n = 7). Data are presented as means ± SD. *P < 0.05; #P < 0.01. NS, not significant.

Article Snippet: Myeloperoxidase (MPO) activity assay was performed as described previously ( 6 ) using the Mouse MPO ELISA kit from Hycult biotech (cat. no. HK210-02) according to the manufacturer’s protocol.

Techniques: Knock-Out, Staining, Activity Assay