Structured Review

Difco mycomedia
Mycomedia, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mycomedia/product/Difco
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mycomedia - by Bioz Stars, 2020-04
85/100 stars

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Modification:

Article Title: Rv1675c (cmr) regulates intramacrophage and cAMP-induced gene expression in Mycobacterium tuberculosis-complex mycobacteria
Article Snippet: M. bovis BCG (Pasteur strain, Trudeau Institute) or Mtb H37Rv (ATCC 25618) was grown in mycomedia (7H9 liquid medium (Difco) supplemented with 0.5% glycerol, 10% oleic acid-dextrose-albumin-catalase, and 0.05% Tween-80). .. The murine macrophage cell line J774.16 was maintained in antibiotic-free Dulbecco’s Modified Eagles’s Medium (DMEM) (Gibco) supplemented with 20% (v/v) fetal bovine serum, 5% (v/v) NCTC-109 (Gibco), 1% (v/v) nonessential amino acids (NEAA) (Gibco), and 1% (v/v) glutamine, as described ( ).

Nucleic Acid Electrophoresis:

Article Title: Identification and Characterization of Mycobacterial Proteins Differentially Expressed under Standing and Shaking Culture Conditions, Including Rv2623 from a Novel Class of Putative ATP-Binding Proteins
Article Snippet: M. bovis BCG (Pasteur strain; Trudeau Institute) was grown in mycomedia (liquid 7H9 medium [Difco] supplemented with 0.5% [vol/vol] glycerol, 10% [vol/vol] oleic acid-albumin-dextrose-catalase [Difco] and 0.05% [vol/vol] Tween 80), as previously described ( ). .. Bacteria were labeled with [35 S]- l -methionine and [35 S]- l -cysteine (Pro-mix; 100 μCi/ml; Amersham) for a 24-h period prior to harvesting the bacteria for 2-D gel electrophoresis.

Labeling:

Article Title: Identification and Characterization of Mycobacterial Proteins Differentially Expressed under Standing and Shaking Culture Conditions, Including Rv2623 from a Novel Class of Putative ATP-Binding Proteins
Article Snippet: Paragraph title: Bacterial growth and labeling conditions. ... M. bovis BCG (Pasteur strain; Trudeau Institute) was grown in mycomedia (liquid 7H9 medium [Difco] supplemented with 0.5% [vol/vol] glycerol, 10% [vol/vol] oleic acid-albumin-dextrose-catalase [Difco] and 0.05% [vol/vol] Tween 80), as previously described ( ).

Expressing:

Article Title: Rv1675c (cmr) regulates intramacrophage and cAMP-induced gene expression in Mycobacterium tuberculosis-complex mycobacteria
Article Snippet: M. bovis BCG (Pasteur strain, Trudeau Institute) or Mtb H37Rv (ATCC 25618) was grown in mycomedia (7H9 liquid medium (Difco) supplemented with 0.5% glycerol, 10% oleic acid-dextrose-albumin-catalase, and 0.05% Tween-80). .. For environmental gene expression assays late log phase cultures were exposed to low pH (mycomedia adjusted to pH 5.5 with 125 mM HCl), hypoxia (1.3 % O2 , 5.0% CO2 ), or nitric oxide (250 μM diethylenetriamine [Sigma]) for 6 h, and gene expression was compared to non-exposed cultures.

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    Difco mycomedia
    Mycomedia, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mycomedia/product/Difco
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mycomedia - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    91
    Difco mycomedica d o o
    Mycomedica D O O, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mycomedica d o o/product/Difco
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mycomedica d o o - by Bioz Stars, 2020-04
    91/100 stars
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