multishot top10  (Thermo Fisher)


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    Name:
    MultiShot TOP10 Chemically Competent E coli
    Description:
    MultiShot TOP10 Chemically Competent E coli provide transformation efficiencies of 1 x 108 cfu µg control plasmid DNA and are suited for high throughput transformations MultiShot TOP10 Chemically Competent cells are packaged in five 96 well microtiter plates 15 µL aliquots to simplify high throughput bacterial transformations Benefits of MultiShot TOP10 cells • Designed for high throughput applications microtiter plate format designed for automated high throughput cloning• Compatible with genomic DNA the mcrA mutation supports efficient transfection of methylated DNA• Permits rapid screening the LacZΔM15 allele permits rapid blue white screening of transfectants on plates containing X Gal or Bluo Gal• Efficient the endA1 mutation helps reduce endonuclease activity improving subsequent plasmid DNA yields• Reliable the recA1 mutation helps reduce unwanted recombinationEasy to use for high throughput transformationsMultiShot TOP10 Chemically Competent cells are genetically similar to the reliable DH10B strain and are available in five 96 well plates to facilitate high throughput transformation After addition of DNA MultiShot TOP10 Chemically Competent cells can be transformed by heat shock at 42°C using a heat block or thermocycler Using our TA Cloning and TOPO Cloning protocols including controls yields of 100 400 colonies per agar plate can be expected Genotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL StrR endA1 nupGFind the strain and format that you needWe also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs If you require other high throughput transformation options choose from our collection of MultiShot formatted comp cells or contact us for custom formatting options
    Catalog Number:
    c40005
    Price:
    None
    Applications:
    Chemically Competent Cells for Cloning|Cloning|Transformation
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher multishot top10
    MultiShot TOP10 Chemically Competent E coli provide transformation efficiencies of 1 x 108 cfu µg control plasmid DNA and are suited for high throughput transformations MultiShot TOP10 Chemically Competent cells are packaged in five 96 well microtiter plates 15 µL aliquots to simplify high throughput bacterial transformations Benefits of MultiShot TOP10 cells • Designed for high throughput applications microtiter plate format designed for automated high throughput cloning• Compatible with genomic DNA the mcrA mutation supports efficient transfection of methylated DNA• Permits rapid screening the LacZΔM15 allele permits rapid blue white screening of transfectants on plates containing X Gal or Bluo Gal• Efficient the endA1 mutation helps reduce endonuclease activity improving subsequent plasmid DNA yields• Reliable the recA1 mutation helps reduce unwanted recombinationEasy to use for high throughput transformationsMultiShot TOP10 Chemically Competent cells are genetically similar to the reliable DH10B strain and are available in five 96 well plates to facilitate high throughput transformation After addition of DNA MultiShot TOP10 Chemically Competent cells can be transformed by heat shock at 42°C using a heat block or thermocycler Using our TA Cloning and TOPO Cloning protocols including controls yields of 100 400 colonies per agar plate can be expected Genotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL StrR endA1 nupGFind the strain and format that you needWe also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs If you require other high throughput transformation options choose from our collection of MultiShot formatted comp cells or contact us for custom formatting options
    https://www.bioz.com/result/multishot top10/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multishot top10 - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
    Article Snippet: .. Purified PCR products were cloned by ligation into a plasmid vector (pCR 2.1TOPO-TA cloning vector, Invitrogen) and transformed into chemically competent E .coli TOP-10 cells, using a TOPO-TA cloning system (Invitrogen) according to the manufacturer’s instructions. .. Amplified PCR products were sequenced using the primer M13f by Macrogen Inc. (Seoul, Republic of Korea).

    Article Title: Communication between Corneal Epithelial Cells and Trigeminal Neurons Is Facilitated by Purinergic (P2) and Glutamatergic Receptors
    Article Snippet: .. The Taq polymerase used was Eppendorf Taqmaster (Eppendorf AG, Hamburg, Germany) under the following conditions: 95°C for 10 min (95°C for 30 s, 60°C for 30 s, 72°C for 1 min)×30 cycles, 72°C for 10 additional min. PCR products were cloned into pCR2.1 vectors (Invitrogen), transformed into TOP10 chemically-competent E.coli (Invitrogen), and cultured on X-gal treated LB/Amp agar plates. .. White colonies were picked, grown in LB/Amp media overnight, and the plasmids purified using Mini-prep kits (Qiagen).

    Ligation:

    Article Title: The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
    Article Snippet: .. Purified PCR products were cloned by ligation into a plasmid vector (pCR 2.1TOPO-TA cloning vector, Invitrogen) and transformed into chemically competent E .coli TOP-10 cells, using a TOPO-TA cloning system (Invitrogen) according to the manufacturer’s instructions. .. Amplified PCR products were sequenced using the primer M13f by Macrogen Inc. (Seoul, Republic of Korea).

    Cell Culture:

    Article Title: Communication between Corneal Epithelial Cells and Trigeminal Neurons Is Facilitated by Purinergic (P2) and Glutamatergic Receptors
    Article Snippet: .. The Taq polymerase used was Eppendorf Taqmaster (Eppendorf AG, Hamburg, Germany) under the following conditions: 95°C for 10 min (95°C for 30 s, 60°C for 30 s, 72°C for 1 min)×30 cycles, 72°C for 10 additional min. PCR products were cloned into pCR2.1 vectors (Invitrogen), transformed into TOP10 chemically-competent E.coli (Invitrogen), and cultured on X-gal treated LB/Amp agar plates. .. White colonies were picked, grown in LB/Amp media overnight, and the plasmids purified using Mini-prep kits (Qiagen).

    Purification:

    Article Title: The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
    Article Snippet: .. Purified PCR products were cloned by ligation into a plasmid vector (pCR 2.1TOPO-TA cloning vector, Invitrogen) and transformed into chemically competent E .coli TOP-10 cells, using a TOPO-TA cloning system (Invitrogen) according to the manufacturer’s instructions. .. Amplified PCR products were sequenced using the primer M13f by Macrogen Inc. (Seoul, Republic of Korea).

    Polymerase Chain Reaction:

    Article Title: The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
    Article Snippet: .. Purified PCR products were cloned by ligation into a plasmid vector (pCR 2.1TOPO-TA cloning vector, Invitrogen) and transformed into chemically competent E .coli TOP-10 cells, using a TOPO-TA cloning system (Invitrogen) according to the manufacturer’s instructions. .. Amplified PCR products were sequenced using the primer M13f by Macrogen Inc. (Seoul, Republic of Korea).

    Article Title: Communication between Corneal Epithelial Cells and Trigeminal Neurons Is Facilitated by Purinergic (P2) and Glutamatergic Receptors
    Article Snippet: .. The Taq polymerase used was Eppendorf Taqmaster (Eppendorf AG, Hamburg, Germany) under the following conditions: 95°C for 10 min (95°C for 30 s, 60°C for 30 s, 72°C for 1 min)×30 cycles, 72°C for 10 additional min. PCR products were cloned into pCR2.1 vectors (Invitrogen), transformed into TOP10 chemically-competent E.coli (Invitrogen), and cultured on X-gal treated LB/Amp agar plates. .. White colonies were picked, grown in LB/Amp media overnight, and the plasmids purified using Mini-prep kits (Qiagen).

    Transformation Assay:

    Article Title: The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
    Article Snippet: .. Purified PCR products were cloned by ligation into a plasmid vector (pCR 2.1TOPO-TA cloning vector, Invitrogen) and transformed into chemically competent E .coli TOP-10 cells, using a TOPO-TA cloning system (Invitrogen) according to the manufacturer’s instructions. .. Amplified PCR products were sequenced using the primer M13f by Macrogen Inc. (Seoul, Republic of Korea).

    Article Title: Communication between Corneal Epithelial Cells and Trigeminal Neurons Is Facilitated by Purinergic (P2) and Glutamatergic Receptors
    Article Snippet: .. The Taq polymerase used was Eppendorf Taqmaster (Eppendorf AG, Hamburg, Germany) under the following conditions: 95°C for 10 min (95°C for 30 s, 60°C for 30 s, 72°C for 1 min)×30 cycles, 72°C for 10 additional min. PCR products were cloned into pCR2.1 vectors (Invitrogen), transformed into TOP10 chemically-competent E.coli (Invitrogen), and cultured on X-gal treated LB/Amp agar plates. .. White colonies were picked, grown in LB/Amp media overnight, and the plasmids purified using Mini-prep kits (Qiagen).

    Plasmid Preparation:

    Article Title: The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
    Article Snippet: .. Purified PCR products were cloned by ligation into a plasmid vector (pCR 2.1TOPO-TA cloning vector, Invitrogen) and transformed into chemically competent E .coli TOP-10 cells, using a TOPO-TA cloning system (Invitrogen) according to the manufacturer’s instructions. .. Amplified PCR products were sequenced using the primer M13f by Macrogen Inc. (Seoul, Republic of Korea).

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  • 84
    Thermo Fisher multishot flexplate top10 competent cells
    Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli <t>Top10</t> cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.
    Multishot Flexplate Top10 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multishot flexplate top10 competent cells/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multishot flexplate top10 competent cells - by Bioz Stars, 2020-07
    84/100 stars
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    Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli Top10 cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.

    Journal: bioRxiv

    Article Title: A novel pH-regulated, unusual 603 bp overlapping protein coding gene pop is encoded antisense to ompA in Escherichia coli O157:H7 (EHEC)

    doi: 10.1101/852251

    Figure Lengend Snippet: Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli Top10 cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.

    Article Snippet: Vector constructs were transformed in E. coli Top10 cells and plated on LB with required antibiotics.

    Techniques: Activity Assay, Plasmid Preparation, Fluorescence, Construct, Agarose Gel Electrophoresis, Binding Assay, Polymerase Chain Reaction