multishot stripwell dh5α t1r competent cells  (Thermo Fisher)


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    Thermo Fisher multishot stripwell dh5α t1r competent cells
    Expression of different glycosyl hydrolase (GH) genes from a common expression vector backbone and test of their effects on growth of E. coli <t>DH5α</t> in MOPS minimal medium ( 17 ) and Z. mobilis ZM4 in Zymomonas minimal medium ( 18 ) with glucose or cellobiose as a carbon source. (A) Expression of GH genes in a pVector backbone and a summary of the constructs and their effects on growth in minimal medium supplemented with cellobiose. (B-D) Growth of E. coli DH5α containing plasmids pVector or pCel3A or pGH3 in MOPS minimal medium supplied with 0.4% glucose or cellobiose. (E-G) Growth of Z. mobilis ZM4 containing plasmids pVector or pCel3A or pGH3 in a Zymomonas minimal medium containing 2% glucose or 2% cellobiose. The growth curves are averages of three replicates. *Gene from Cellvibrio japonicus , # Gene from Caulobacter crescentus .
    Multishot Stripwell Dh5α T1r Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multishot stripwell dh5α t1r competent cells/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multishot stripwell dh5α t1r competent cells - by Bioz Stars, 2020-08
    84/100 stars

    Images

    1) Product Images from "Heterologous glycosyl hydrolase expression and cellular reprogramming resembling sucrose-induction enable Zymomonas mobilis growth on cellobiose"

    Article Title: Heterologous glycosyl hydrolase expression and cellular reprogramming resembling sucrose-induction enable Zymomonas mobilis growth on cellobiose

    Journal: bioRxiv

    doi: 10.1101/854646

    Expression of different glycosyl hydrolase (GH) genes from a common expression vector backbone and test of their effects on growth of E. coli DH5α in MOPS minimal medium ( 17 ) and Z. mobilis ZM4 in Zymomonas minimal medium ( 18 ) with glucose or cellobiose as a carbon source. (A) Expression of GH genes in a pVector backbone and a summary of the constructs and their effects on growth in minimal medium supplemented with cellobiose. (B-D) Growth of E. coli DH5α containing plasmids pVector or pCel3A or pGH3 in MOPS minimal medium supplied with 0.4% glucose or cellobiose. (E-G) Growth of Z. mobilis ZM4 containing plasmids pVector or pCel3A or pGH3 in a Zymomonas minimal medium containing 2% glucose or 2% cellobiose. The growth curves are averages of three replicates. *Gene from Cellvibrio japonicus , # Gene from Caulobacter crescentus .
    Figure Legend Snippet: Expression of different glycosyl hydrolase (GH) genes from a common expression vector backbone and test of their effects on growth of E. coli DH5α in MOPS minimal medium ( 17 ) and Z. mobilis ZM4 in Zymomonas minimal medium ( 18 ) with glucose or cellobiose as a carbon source. (A) Expression of GH genes in a pVector backbone and a summary of the constructs and their effects on growth in minimal medium supplemented with cellobiose. (B-D) Growth of E. coli DH5α containing plasmids pVector or pCel3A or pGH3 in MOPS minimal medium supplied with 0.4% glucose or cellobiose. (E-G) Growth of Z. mobilis ZM4 containing plasmids pVector or pCel3A or pGH3 in a Zymomonas minimal medium containing 2% glucose or 2% cellobiose. The growth curves are averages of three replicates. *Gene from Cellvibrio japonicus , # Gene from Caulobacter crescentus .

    Techniques Used: Expressing, Plasmid Preparation, Construct

    2) Product Images from "Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. coli"

    Article Title: Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. coli

    Journal: bioRxiv

    doi: 10.1101/819920

    YegI is an integral membrane protein A) Predicted topology of YegI using Phobius. Amino acid positions at which phoA-lacZα reporters are fused are indicated along with associated phenotypes. Phenotypes of phoAlacZα fusions due to LacZ activity are denoted as red ellipses while PhoA activity are denoted as blue ellipses. Predicted transmembrane domains (I and II) are depicted as grey rectangles. (B) Membrane topology analysis: DH5α transformed with different phoAlacZα reporters were streaked on an LB plate containing 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos, 80μg/ml), 6-chloro-3-indolyl-β-D-galactoside (Red-Gal, 100 μg/ml, 0.1 % w/v arabinose and 100 μg/ml of ampicillin. Control pBAD24: control strain expressing pBAD24 empty vector, control phoAlacZα: strain expressing phoAlacZα in pBAD24 without YegI. Amino acid positions at which phoAlacZα reporters are fused are indicated.
    Figure Legend Snippet: YegI is an integral membrane protein A) Predicted topology of YegI using Phobius. Amino acid positions at which phoA-lacZα reporters are fused are indicated along with associated phenotypes. Phenotypes of phoAlacZα fusions due to LacZ activity are denoted as red ellipses while PhoA activity are denoted as blue ellipses. Predicted transmembrane domains (I and II) are depicted as grey rectangles. (B) Membrane topology analysis: DH5α transformed with different phoAlacZα reporters were streaked on an LB plate containing 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos, 80μg/ml), 6-chloro-3-indolyl-β-D-galactoside (Red-Gal, 100 μg/ml, 0.1 % w/v arabinose and 100 μg/ml of ampicillin. Control pBAD24: control strain expressing pBAD24 empty vector, control phoAlacZα: strain expressing phoAlacZα in pBAD24 without YegI. Amino acid positions at which phoAlacZα reporters are fused are indicated.

    Techniques Used: Activity Assay, Transformation Assay, Expressing, Plasmid Preparation

    Related Articles

    Clone Assay:

    Article Title: Heterologous glycosyl hydrolase expression and cellular reprogramming resembling sucrose-induction enable Zymomonas mobilis growth on cellobiose
    Article Snippet: .. The E. coli DH10B strain was used for cloning and E. coli DH5α was used for expressing the recombinant plasmids. ..

    Article Title: Interaction of 14-3-3I and CDPK1 mediates the growth of human malaria parasite
    Article Snippet: .. Recombinant plasmid was transformed into Escherichia coli DH5α competent cells, and positive clones were confirmed by restriction digestion of recombinant plasmid and Sanger sequencing of insert DNA fragment. .. For over-expression of GST-14-3-3I fusion protein, the recombinant plasmid containing 14-3-3I gene was transformed into E. coli strain Rosetta competent cells.

    Ligation:

    Article Title: Structure and function of the bacterial protein toxin phenomycin
    Article Snippet: .. Upon ligation with T4 DNA ligase (#EL0011), vector and insert were mixed in a 1:3 molar ratio using 50 ng of pETM-11 and incubated for 30 minutes at room temperature, before transforming chemically competent E. coli DH5α with ligation mixture. .. 3-5μl of the ligation mixture was transferred to DH5α (50μl) and incubated 30 min on ice, followed by heat shock (42°C) for 50 seconds and 2 min incubation on ice.

    Article Title: Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. coli
    Article Snippet: .. Ligation products were transformed in DH5α cells and selected on LB/ampicillin plates. .. The resulting plasmid pKR31 generated an N-terminal His6 -tagged YegI fusion protein.

    Incubation:

    Article Title: Structure and function of the bacterial protein toxin phenomycin
    Article Snippet: .. Upon ligation with T4 DNA ligase (#EL0011), vector and insert were mixed in a 1:3 molar ratio using 50 ng of pETM-11 and incubated for 30 minutes at room temperature, before transforming chemically competent E. coli DH5α with ligation mixture. .. 3-5μl of the ligation mixture was transferred to DH5α (50μl) and incubated 30 min on ice, followed by heat shock (42°C) for 50 seconds and 2 min incubation on ice.

    Expressing:

    Article Title: Heterologous glycosyl hydrolase expression and cellular reprogramming resembling sucrose-induction enable Zymomonas mobilis growth on cellobiose
    Article Snippet: .. The E. coli DH10B strain was used for cloning and E. coli DH5α was used for expressing the recombinant plasmids. ..

    Sequencing:

    Article Title: Interaction of 14-3-3I and CDPK1 mediates the growth of human malaria parasite
    Article Snippet: .. Recombinant plasmid was transformed into Escherichia coli DH5α competent cells, and positive clones were confirmed by restriction digestion of recombinant plasmid and Sanger sequencing of insert DNA fragment. .. For over-expression of GST-14-3-3I fusion protein, the recombinant plasmid containing 14-3-3I gene was transformed into E. coli strain Rosetta competent cells.

    Transformation Assay:

    Article Title: HIV-1 mutations in HIV-1 Gag, protease, RT p66 and when they appear: Insights from an in vitro BSL2 assay on mutation rates and types
    Article Snippet: .. Transformed DH5α cells were plated and grown overnight at 37°C on LB agar plates supplemented with kanamycin (50 μg/ml). .. Transformants were screened using GoTaq PCR (Promega) with universal M13F (−20) forward and reverse primers prior to sequencing.

    Article Title: Beyond target-decoy competition: stable validation of peptide and protein identifications in mass spectrometry-based discovery proteomics
    Article Snippet: .. Briefly, competent E. coli DH5α cells transformed with pUC19 plasmid were grown at 37°C in LB medium containing carbenicillin before harvesting during exponential phase (OD600 ~ 0.6). .. After centrifugation at 3′000 × g during 10 min, the pellet was washed 3 times with cold PBS before lysis of cells using Bugbuster Protein Extraction Reagent (Novagen) containing cOmplcte™, EDTA-free Protease Inhibitor Cocktail (Roche) and benzonase (Merck Millipore).

    Article Title: Interaction of 14-3-3I and CDPK1 mediates the growth of human malaria parasite
    Article Snippet: .. Recombinant plasmid was transformed into Escherichia coli DH5α competent cells, and positive clones were confirmed by restriction digestion of recombinant plasmid and Sanger sequencing of insert DNA fragment. .. For over-expression of GST-14-3-3I fusion protein, the recombinant plasmid containing 14-3-3I gene was transformed into E. coli strain Rosetta competent cells.

    Article Title: The lupus susceptibility locus Sgp3 encodes the suppressor of endogenous retrovirus expression SNERV
    Article Snippet: .. Competent DH5α cells were transformed, plated onto LB-agar plates containing kanamycin, and grown overnight at 37 degrees Celsius. .. Colonies were selected and grown overnight in 3mL of LB-kanamycin and plasmids were isolated using the QIAprep Spin Miniprep Kit (Qiagen) and sequenced.

    Article Title: Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. coli
    Article Snippet: .. Ligation products were transformed in DH5α cells and selected on LB/ampicillin plates. .. The resulting plasmid pKR31 generated an N-terminal His6 -tagged YegI fusion protein.

    Recombinant:

    Article Title: Heterologous glycosyl hydrolase expression and cellular reprogramming resembling sucrose-induction enable Zymomonas mobilis growth on cellobiose
    Article Snippet: .. The E. coli DH10B strain was used for cloning and E. coli DH5α was used for expressing the recombinant plasmids. ..

    Article Title: Interaction of 14-3-3I and CDPK1 mediates the growth of human malaria parasite
    Article Snippet: .. Recombinant plasmid was transformed into Escherichia coli DH5α competent cells, and positive clones were confirmed by restriction digestion of recombinant plasmid and Sanger sequencing of insert DNA fragment. .. For over-expression of GST-14-3-3I fusion protein, the recombinant plasmid containing 14-3-3I gene was transformed into E. coli strain Rosetta competent cells.

    Plasmid Preparation:

    Article Title: Central role and structure of the membrane pseudokinase YukC in the antibacterial Bacillus subtilis Type VIIb Secretion System
    Article Snippet: .. Plasmid propagation and construction were routinely performed with E. coli DH5α. .. Genes of interest were amplified with the Phusion polymerase (Thermo Scientific) using purified B. subtilis 168 DNA as template and cloned with standard restriction enzymes-based procedures.

    Article Title: Beyond target-decoy competition: stable validation of peptide and protein identifications in mass spectrometry-based discovery proteomics
    Article Snippet: .. Briefly, competent E. coli DH5α cells transformed with pUC19 plasmid were grown at 37°C in LB medium containing carbenicillin before harvesting during exponential phase (OD600 ~ 0.6). .. After centrifugation at 3′000 × g during 10 min, the pellet was washed 3 times with cold PBS before lysis of cells using Bugbuster Protein Extraction Reagent (Novagen) containing cOmplcte™, EDTA-free Protease Inhibitor Cocktail (Roche) and benzonase (Merck Millipore).

    Article Title: Structure and function of the bacterial protein toxin phenomycin
    Article Snippet: .. Upon ligation with T4 DNA ligase (#EL0011), vector and insert were mixed in a 1:3 molar ratio using 50 ng of pETM-11 and incubated for 30 minutes at room temperature, before transforming chemically competent E. coli DH5α with ligation mixture. .. 3-5μl of the ligation mixture was transferred to DH5α (50μl) and incubated 30 min on ice, followed by heat shock (42°C) for 50 seconds and 2 min incubation on ice.

    Article Title: Interaction of 14-3-3I and CDPK1 mediates the growth of human malaria parasite
    Article Snippet: .. Recombinant plasmid was transformed into Escherichia coli DH5α competent cells, and positive clones were confirmed by restriction digestion of recombinant plasmid and Sanger sequencing of insert DNA fragment. .. For over-expression of GST-14-3-3I fusion protein, the recombinant plasmid containing 14-3-3I gene was transformed into E. coli strain Rosetta competent cells.

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  • 84
    Thermo Fisher multishot stripwell dh5α t1r competent cells
    Expression of different glycosyl hydrolase (GH) genes from a common expression vector backbone and test of their effects on growth of E. coli <t>DH5α</t> in MOPS minimal medium ( 17 ) and Z. mobilis ZM4 in Zymomonas minimal medium ( 18 ) with glucose or cellobiose as a carbon source. (A) Expression of GH genes in a pVector backbone and a summary of the constructs and their effects on growth in minimal medium supplemented with cellobiose. (B-D) Growth of E. coli DH5α containing plasmids pVector or pCel3A or pGH3 in MOPS minimal medium supplied with 0.4% glucose or cellobiose. (E-G) Growth of Z. mobilis ZM4 containing plasmids pVector or pCel3A or pGH3 in a Zymomonas minimal medium containing 2% glucose or 2% cellobiose. The growth curves are averages of three replicates. *Gene from Cellvibrio japonicus , # Gene from Caulobacter crescentus .
    Multishot Stripwell Dh5α T1r Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multishot stripwell dh5α t1r competent cells/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multishot stripwell dh5α t1r competent cells - by Bioz Stars, 2020-08
    84/100 stars
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    Expression of different glycosyl hydrolase (GH) genes from a common expression vector backbone and test of their effects on growth of E. coli DH5α in MOPS minimal medium ( 17 ) and Z. mobilis ZM4 in Zymomonas minimal medium ( 18 ) with glucose or cellobiose as a carbon source. (A) Expression of GH genes in a pVector backbone and a summary of the constructs and their effects on growth in minimal medium supplemented with cellobiose. (B-D) Growth of E. coli DH5α containing plasmids pVector or pCel3A or pGH3 in MOPS minimal medium supplied with 0.4% glucose or cellobiose. (E-G) Growth of Z. mobilis ZM4 containing plasmids pVector or pCel3A or pGH3 in a Zymomonas minimal medium containing 2% glucose or 2% cellobiose. The growth curves are averages of three replicates. *Gene from Cellvibrio japonicus , # Gene from Caulobacter crescentus .

    Journal: bioRxiv

    Article Title: Heterologous glycosyl hydrolase expression and cellular reprogramming resembling sucrose-induction enable Zymomonas mobilis growth on cellobiose

    doi: 10.1101/854646

    Figure Lengend Snippet: Expression of different glycosyl hydrolase (GH) genes from a common expression vector backbone and test of their effects on growth of E. coli DH5α in MOPS minimal medium ( 17 ) and Z. mobilis ZM4 in Zymomonas minimal medium ( 18 ) with glucose or cellobiose as a carbon source. (A) Expression of GH genes in a pVector backbone and a summary of the constructs and their effects on growth in minimal medium supplemented with cellobiose. (B-D) Growth of E. coli DH5α containing plasmids pVector or pCel3A or pGH3 in MOPS minimal medium supplied with 0.4% glucose or cellobiose. (E-G) Growth of Z. mobilis ZM4 containing plasmids pVector or pCel3A or pGH3 in a Zymomonas minimal medium containing 2% glucose or 2% cellobiose. The growth curves are averages of three replicates. *Gene from Cellvibrio japonicus , # Gene from Caulobacter crescentus .

    Article Snippet: The E. coli DH10B strain was used for cloning and E. coli DH5α was used for expressing the recombinant plasmids.

    Techniques: Expressing, Plasmid Preparation, Construct

    YegI is an integral membrane protein A) Predicted topology of YegI using Phobius. Amino acid positions at which phoA-lacZα reporters are fused are indicated along with associated phenotypes. Phenotypes of phoAlacZα fusions due to LacZ activity are denoted as red ellipses while PhoA activity are denoted as blue ellipses. Predicted transmembrane domains (I and II) are depicted as grey rectangles. (B) Membrane topology analysis: DH5α transformed with different phoAlacZα reporters were streaked on an LB plate containing 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos, 80μg/ml), 6-chloro-3-indolyl-β-D-galactoside (Red-Gal, 100 μg/ml, 0.1 % w/v arabinose and 100 μg/ml of ampicillin. Control pBAD24: control strain expressing pBAD24 empty vector, control phoAlacZα: strain expressing phoAlacZα in pBAD24 without YegI. Amino acid positions at which phoAlacZα reporters are fused are indicated.

    Journal: bioRxiv

    Article Title: Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. coli

    doi: 10.1101/819920

    Figure Lengend Snippet: YegI is an integral membrane protein A) Predicted topology of YegI using Phobius. Amino acid positions at which phoA-lacZα reporters are fused are indicated along with associated phenotypes. Phenotypes of phoAlacZα fusions due to LacZ activity are denoted as red ellipses while PhoA activity are denoted as blue ellipses. Predicted transmembrane domains (I and II) are depicted as grey rectangles. (B) Membrane topology analysis: DH5α transformed with different phoAlacZα reporters were streaked on an LB plate containing 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos, 80μg/ml), 6-chloro-3-indolyl-β-D-galactoside (Red-Gal, 100 μg/ml, 0.1 % w/v arabinose and 100 μg/ml of ampicillin. Control pBAD24: control strain expressing pBAD24 empty vector, control phoAlacZα: strain expressing phoAlacZα in pBAD24 without YegI. Amino acid positions at which phoAlacZα reporters are fused are indicated.

    Article Snippet: Ligation products were transformed in DH5α cells and selected on LB/ampicillin plates.

    Techniques: Activity Assay, Transformation Assay, Expressing, Plasmid Preparation