multiplex rna single nucleotide primer extension snupe assays  (Sequenom)

 
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    Sequenom multiplex rna single nucleotide primer extension snupe assays
    Testing for transgenerational epigenetic inheritance of the aberrant imprinted expression. (A) Breeding design to test whether ED-perturbed parental allele-specific transcription is transgenerationally inherited through the paternal germline to an unexposed generation. G1 male fetuses were exposed in utero to EDs or vehicle control (‘oil’) daily from 12.5 dpc to 16.5 dpc. After reaching adulthood, 129S1 G1 males were mated with 129S1 unexposed females to generate G2 offspring (3 blue stars), which derived from exposed prospermatogonia. At adulthood, G2 males were mated with unexposed JF1 females to generate G3 offspring, which were never directly exposed to EDs. JF1 × 129 G3 fetuses were dissected at 13.5 dpc to collect organs for <t>RNA</t> isolation. Parental-specific transcription was quantified in the total RNA using multiplex <t>SNuPE</t> assays. (B) Results of Sequenom allelotyping experiments using heart and lung tissue of the G3 generation; color scale as in Figure 2 ; letters in parentheses denote independent SNPs. Notice the lack of inherited changes from the exposed generation. More groups of fetuses are shown in Additional file 2 . This Figure includes standards that are routinely included in the Sequenom runs (see Methods).
    Multiplex Rna Single Nucleotide Primer Extension Snupe Assays, supplied by Sequenom, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex rna single nucleotide primer extension snupe assays/product/Sequenom
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multiplex rna single nucleotide primer extension snupe assays - by Bioz Stars, 2020-04
    88/100 stars

    Related Products / Commonly Used Together

    allele-specific transcription
    jf1
    og2 cells

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    1) Product Images from "Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming"

    Article Title: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

    Journal: Genome Biology

    doi: 10.1186/s13059-015-0619-z

    Testing for transgenerational epigenetic inheritance of the aberrant imprinted expression. (A) Breeding design to test whether ED-perturbed parental allele-specific transcription is transgenerationally inherited through the paternal germline to an unexposed generation. G1 male fetuses were exposed in utero to EDs or vehicle control (‘oil’) daily from 12.5 dpc to 16.5 dpc. After reaching adulthood, 129S1 G1 males were mated with 129S1 unexposed females to generate G2 offspring (3 blue stars), which derived from exposed prospermatogonia. At adulthood, G2 males were mated with unexposed JF1 females to generate G3 offspring, which were never directly exposed to EDs. JF1 × 129 G3 fetuses were dissected at 13.5 dpc to collect organs for RNA isolation. Parental-specific transcription was quantified in the total RNA using multiplex SNuPE assays. (B) Results of Sequenom allelotyping experiments using heart and lung tissue of the G3 generation; color scale as in Figure 2 ; letters in parentheses denote independent SNPs. Notice the lack of inherited changes from the exposed generation. More groups of fetuses are shown in Additional file 2 . This Figure includes standards that are routinely included in the Sequenom runs (see Methods).
    Figure Legend Snippet: Testing for transgenerational epigenetic inheritance of the aberrant imprinted expression. (A) Breeding design to test whether ED-perturbed parental allele-specific transcription is transgenerationally inherited through the paternal germline to an unexposed generation. G1 male fetuses were exposed in utero to EDs or vehicle control (‘oil’) daily from 12.5 dpc to 16.5 dpc. After reaching adulthood, 129S1 G1 males were mated with 129S1 unexposed females to generate G2 offspring (3 blue stars), which derived from exposed prospermatogonia. At adulthood, G2 males were mated with unexposed JF1 females to generate G3 offspring, which were never directly exposed to EDs. JF1 × 129 G3 fetuses were dissected at 13.5 dpc to collect organs for RNA isolation. Parental-specific transcription was quantified in the total RNA using multiplex SNuPE assays. (B) Results of Sequenom allelotyping experiments using heart and lung tissue of the G3 generation; color scale as in Figure 2 ; letters in parentheses denote independent SNPs. Notice the lack of inherited changes from the exposed generation. More groups of fetuses are shown in Additional file 2 . This Figure includes standards that are routinely included in the Sequenom runs (see Methods).

    Techniques Used: Expressing, In Utero, Derivative Assay, Isolation, Multiplex Assay

    Related Articles

    Transgenic Assay:

    Article Title: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming
    Article Snippet: The OG2 transgenic line carries an Oct4 promoter-GFP transgene that allowed us to purify the GFP-positive male and female germ cells (MGCs and FGCs) and GFP-negative somatic cells (MSCs and FSCs) from the dissected embryonic gonads by FACS sorting (Figure B). .. This allowed measurement of the allele-specific transcription of known imprinted genes in JF1 × OG2 cells using multiplex RNA-single nucleotide primer extension (SNuPE) assays uisng Sequenom allelotyping [ ].

    Multiplex Assay:

    Article Title: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming
    Article Snippet: .. This allowed measurement of the allele-specific transcription of known imprinted genes in JF1 × OG2 cells using multiplex RNA-single nucleotide primer extension (SNuPE) assays uisng Sequenom allelotyping [ ]. ..

    Expressing:

    Article Title: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming
    Article Snippet: This allowed measurement of the allele-specific transcription of known imprinted genes in JF1 × OG2 cells using multiplex RNA-single nucleotide primer extension (SNuPE) assays uisng Sequenom allelotyping [ ]. .. These expression patterns represent the normal correct ubiquitous parental allele-specific transcription.

    FACS:

    Article Title: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming
    Article Snippet: The OG2 transgenic line carries an Oct4 promoter-GFP transgene that allowed us to purify the GFP-positive male and female germ cells (MGCs and FGCs) and GFP-negative somatic cells (MSCs and FSCs) from the dissected embryonic gonads by FACS sorting (Figure B). .. This allowed measurement of the allele-specific transcription of known imprinted genes in JF1 × OG2 cells using multiplex RNA-single nucleotide primer extension (SNuPE) assays uisng Sequenom allelotyping [ ].

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    Sequenom multiplex rna single nucleotide primer extension snupe assays
    Testing for transgenerational epigenetic inheritance of the aberrant imprinted expression. (A) Breeding design to test whether ED-perturbed parental allele-specific transcription is transgenerationally inherited through the paternal germline to an unexposed generation. G1 male fetuses were exposed in utero to EDs or vehicle control (‘oil’) daily from 12.5 dpc to 16.5 dpc. After reaching adulthood, 129S1 G1 males were mated with 129S1 unexposed females to generate G2 offspring (3 blue stars), which derived from exposed prospermatogonia. At adulthood, G2 males were mated with unexposed JF1 females to generate G3 offspring, which were never directly exposed to EDs. JF1 × 129 G3 fetuses were dissected at 13.5 dpc to collect organs for <t>RNA</t> isolation. Parental-specific transcription was quantified in the total RNA using multiplex <t>SNuPE</t> assays. (B) Results of Sequenom allelotyping experiments using heart and lung tissue of the G3 generation; color scale as in Figure 2 ; letters in parentheses denote independent SNPs. Notice the lack of inherited changes from the exposed generation. More groups of fetuses are shown in Additional file 2 . This Figure includes standards that are routinely included in the Sequenom runs (see Methods).
    Multiplex Rna Single Nucleotide Primer Extension Snupe Assays, supplied by Sequenom, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex rna single nucleotide primer extension snupe assays/product/Sequenom
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multiplex rna single nucleotide primer extension snupe assays - by Bioz Stars, 2020-04
    88/100 stars
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    Testing for transgenerational epigenetic inheritance of the aberrant imprinted expression. (A) Breeding design to test whether ED-perturbed parental allele-specific transcription is transgenerationally inherited through the paternal germline to an unexposed generation. G1 male fetuses were exposed in utero to EDs or vehicle control (‘oil’) daily from 12.5 dpc to 16.5 dpc. After reaching adulthood, 129S1 G1 males were mated with 129S1 unexposed females to generate G2 offspring (3 blue stars), which derived from exposed prospermatogonia. At adulthood, G2 males were mated with unexposed JF1 females to generate G3 offspring, which were never directly exposed to EDs. JF1 × 129 G3 fetuses were dissected at 13.5 dpc to collect organs for RNA isolation. Parental-specific transcription was quantified in the total RNA using multiplex SNuPE assays. (B) Results of Sequenom allelotyping experiments using heart and lung tissue of the G3 generation; color scale as in Figure 2 ; letters in parentheses denote independent SNPs. Notice the lack of inherited changes from the exposed generation. More groups of fetuses are shown in Additional file 2 . This Figure includes standards that are routinely included in the Sequenom runs (see Methods).

    Journal: Genome Biology

    Article Title: Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming

    doi: 10.1186/s13059-015-0619-z

    Figure Lengend Snippet: Testing for transgenerational epigenetic inheritance of the aberrant imprinted expression. (A) Breeding design to test whether ED-perturbed parental allele-specific transcription is transgenerationally inherited through the paternal germline to an unexposed generation. G1 male fetuses were exposed in utero to EDs or vehicle control (‘oil’) daily from 12.5 dpc to 16.5 dpc. After reaching adulthood, 129S1 G1 males were mated with 129S1 unexposed females to generate G2 offspring (3 blue stars), which derived from exposed prospermatogonia. At adulthood, G2 males were mated with unexposed JF1 females to generate G3 offspring, which were never directly exposed to EDs. JF1 × 129 G3 fetuses were dissected at 13.5 dpc to collect organs for RNA isolation. Parental-specific transcription was quantified in the total RNA using multiplex SNuPE assays. (B) Results of Sequenom allelotyping experiments using heart and lung tissue of the G3 generation; color scale as in Figure 2 ; letters in parentheses denote independent SNPs. Notice the lack of inherited changes from the exposed generation. More groups of fetuses are shown in Additional file 2 . This Figure includes standards that are routinely included in the Sequenom runs (see Methods).

    Article Snippet: This allowed measurement of the allele-specific transcription of known imprinted genes in JF1 × OG2 cells using multiplex RNA-single nucleotide primer extension (SNuPE) assays uisng Sequenom allelotyping [ ].

    Techniques: Expressing, In Utero, Derivative Assay, Isolation, Multiplex Assay