multiplex pcr kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Qiagen multiplex pcr kit
    Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 994 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex pcr kit/product/Qiagen
    Average 99 stars, based on 994 article reviews
    Price from $9.99 to $1999.99
    multiplex pcr kit - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. For these, the relevant amplicon from a separate, non-multiplex PCR was cloned into the pGEM-T Easy vector (Promega, Madison, WI) and sequenced.

    Multiplex Assay:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: .. Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. Amplified DNA was purified with the Qiaquick PCR Purification Kit (Qiagen) and sequenced.

    Amplification:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. Amplified DNA was purified with the Qiaquick PCR Purification Kit (Qiagen) and sequenced.

    Genomic Sequencing:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. Gap software was used to compare sequences to NCBI genomic sequences.

    Immunohistochemistry:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: MHCII status for these samples was determined by immunohistochemistry. .. Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen).

    Purification:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. Amplified DNA was purified with the Qiaquick PCR Purification Kit (Qiagen) and sequenced.

    Expressing:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Fifteen LLMPP cases were de novo MHCII+ samples in the upper 25–100% of expression. .. Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: .. Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. Amplified DNA was purified with the Qiaquick PCR Purification Kit (Qiagen) and sequenced.

    Plasmid Preparation:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. For these, the relevant amplicon from a separate, non-multiplex PCR was cloned into the pGEM-T Easy vector (Promega, Madison, WI) and sequenced.

    Software:

    Article Title: CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
    Article Snippet: Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). .. Gap software was used to compare sequences to NCBI genomic sequences.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Qiagen type it microsatellite pcr kit
    Type It Microsatellite Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type it microsatellite pcr kit/product/Qiagen
    Average 90 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    type it microsatellite pcr kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Qiagen rotor gene multiplex pcr kit
    Genotyping of chimeric mice a , Chromatogram of <t>mtDNA</t> depicting sequence differences at base pairs (bp) 11939, 12062 and 12122 and demonstrating that mtDNA in ntESCs was derived from recipient embryo cytoplasm. Sequence differences between chimera host embryos and ntESCs at 11939, 12199, 12002, 12005, 12008 and 12062 bp allowed quantification of chimerism. The mtDNA from 2N chimeric mouse appeared as a double peak. The 4N chimera showed only ntESC mtDNA (GeneBank AY172335.1). b , Allele Refractory Mutation System quantitative <t>PCR</t> (ARMS-qPCR) assay in 2N and 4N chimeras. Similar colors indicate similar origin. Distances between color plots represent the contribution of chimeric host embryos. Amplification plot (left) and bar graph (right). Detection limit of this assay is 0.01%. c , High ntESC contribution chimeric female and her pups demonstrating germline transmission. White spot on the head of the dam originated from the ICR host embryo. d , Tetraploid (4N) embryo complementation pups born after ntESC (black) injection into host 8-cell stage 4N embryos. e , Genotyping of 4N complementation pups.
    Rotor Gene Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rotor gene multiplex pcr kit/product/Qiagen
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    rotor gene multiplex pcr kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Qiagen quantifast multiplex rt pcr kit
    Genotyping of chimeric mice a , Chromatogram of <t>mtDNA</t> depicting sequence differences at base pairs (bp) 11939, 12062 and 12122 and demonstrating that mtDNA in ntESCs was derived from recipient embryo cytoplasm. Sequence differences between chimera host embryos and ntESCs at 11939, 12199, 12002, 12005, 12008 and 12062 bp allowed quantification of chimerism. The mtDNA from 2N chimeric mouse appeared as a double peak. The 4N chimera showed only ntESC mtDNA (GeneBank AY172335.1). b , Allele Refractory Mutation System quantitative <t>PCR</t> (ARMS-qPCR) assay in 2N and 4N chimeras. Similar colors indicate similar origin. Distances between color plots represent the contribution of chimeric host embryos. Amplification plot (left) and bar graph (right). Detection limit of this assay is 0.01%. c , High ntESC contribution chimeric female and her pups demonstrating germline transmission. White spot on the head of the dam originated from the ICR host embryo. d , Tetraploid (4N) embryo complementation pups born after ntESC (black) injection into host 8-cell stage 4N embryos. e , Genotyping of 4N complementation pups.
    Quantifast Multiplex Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantifast multiplex rt pcr kit/product/Qiagen
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    quantifast multiplex rt pcr kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results


    Genotyping of chimeric mice a , Chromatogram of mtDNA depicting sequence differences at base pairs (bp) 11939, 12062 and 12122 and demonstrating that mtDNA in ntESCs was derived from recipient embryo cytoplasm. Sequence differences between chimera host embryos and ntESCs at 11939, 12199, 12002, 12005, 12008 and 12062 bp allowed quantification of chimerism. The mtDNA from 2N chimeric mouse appeared as a double peak. The 4N chimera showed only ntESC mtDNA (GeneBank AY172335.1). b , Allele Refractory Mutation System quantitative PCR (ARMS-qPCR) assay in 2N and 4N chimeras. Similar colors indicate similar origin. Distances between color plots represent the contribution of chimeric host embryos. Amplification plot (left) and bar graph (right). Detection limit of this assay is 0.01%. c , High ntESC contribution chimeric female and her pups demonstrating germline transmission. White spot on the head of the dam originated from the ICR host embryo. d , Tetraploid (4N) embryo complementation pups born after ntESC (black) injection into host 8-cell stage 4N embryos. e , Genotyping of 4N complementation pups.

    Journal: Nature

    Article Title: Nuclear reprogramming by interphase cytoplasm of 2-cell mouse embryos

    doi: 10.1038/nature13134

    Figure Lengend Snippet: Genotyping of chimeric mice a , Chromatogram of mtDNA depicting sequence differences at base pairs (bp) 11939, 12062 and 12122 and demonstrating that mtDNA in ntESCs was derived from recipient embryo cytoplasm. Sequence differences between chimera host embryos and ntESCs at 11939, 12199, 12002, 12005, 12008 and 12062 bp allowed quantification of chimerism. The mtDNA from 2N chimeric mouse appeared as a double peak. The 4N chimera showed only ntESC mtDNA (GeneBank AY172335.1). b , Allele Refractory Mutation System quantitative PCR (ARMS-qPCR) assay in 2N and 4N chimeras. Similar colors indicate similar origin. Distances between color plots represent the contribution of chimeric host embryos. Amplification plot (left) and bar graph (right). Detection limit of this assay is 0.01%. c , High ntESC contribution chimeric female and her pups demonstrating germline transmission. White spot on the head of the dam originated from the ICR host embryo. d , Tetraploid (4N) embryo complementation pups born after ntESC (black) injection into host 8-cell stage 4N embryos. e , Genotyping of 4N complementation pups.

    Article Snippet: CO3 (common mtDNA region)-TET (yellow) and ND5 (wild specific mtDNA region)-FAM (blue) were mixed and measured with Rotor-Gene Multiplex PCR Kit (Qiagen).

    Techniques: Mouse Assay, Sequencing, Derivative Assay, Mutagenesis, Real-time Polymerase Chain Reaction, Amplification, Transmission Assay, Injection