multiplex oligos  (New England Biolabs)


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    Structured Review

    New England Biolabs multiplex oligos
    Multiplex Oligos, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex oligos/product/New England Biolabs
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    multiplex oligos - by Bioz Stars, 2020-01
    86/100 stars

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    Multiplex Assay:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA). .. Samples were size-selected with Ampure XP beads on the SX-8G-IP-Star Compact robot (Diagenode) using a total of 90 μL beads per sample and eluted in 25 μL DNase-free H2 O.

    Methylation:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: The reaction mix was purified using Ampure XP beads (Beckman-Coulter, CA, USA) after which methylated DNA immunoprecipitation (MeDIP) was set up on the SX-8G-IP-Star Compact robot (Diagenode) according to manufacturer’s instructions applying the Auto MeDIP kit (Diagenode) and including unmethylated and methylated spike-in controls. .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

    Methylated DNA Immunoprecipitation Sequencing:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Paragraph title: MeDIP-seq ... Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

    Purification:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Antibody incubation was performed at 4 °C for 15 h. Immunoprecipitated samples were magnetically purified on the robot using the Auto iPure v2 kit (Diagenode) according to the manufacturer’s instructions. .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Recovery and enrichment was evaluated by qPCR using primer sets specific for the spike-in controls. .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

    Immunoprecipitation:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Antibody incubation was performed at 4 °C for 15 h. Immunoprecipitated samples were magnetically purified on the robot using the Auto iPure v2 kit (Diagenode) according to the manufacturer’s instructions. .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

    Incubation:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Antibody incubation was performed at 4 °C for 15 h. Immunoprecipitated samples were magnetically purified on the robot using the Auto iPure v2 kit (Diagenode) according to the manufacturer’s instructions. .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA). .. Samples were size-selected with Ampure XP beads on the SX-8G-IP-Star Compact robot (Diagenode) using a total of 90 μL beads per sample and eluted in 25 μL DNase-free H2 O.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA). .. Post-amplification enrichment was verified by qPCR using primer pairs targeting the endogenous hypermethylated promoter region of testis specific histone 2B (TSH2B ) (Diagenode, cat.nr.

    Methylated DNA Immunoprecipitation:

    Article Title: Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Article Snippet: Effectively between 60 and 70 ng of adaptor-ligated DNA was used in the MeDIP process. .. Based on the recovery rate, samples were PCR-amplified at 13 cycles (oDBS) or 14 cycles (hDBS and rDBS) using multiplex oligos (New England Biolabs, MA, USA).

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