gaussian multipeaks fit  (OriginLab corp)

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Bottom-up single-molecule strategy for understanding subunit function of tetrameric β-galactosidase

doi: 10.1073/pnas.1805690115

Figure Lengend Snippet: Statistical analysis of β-gal populations. (A) Histogram of β-gal activities. T1∼T4 labels indicate the number of active monomers in the expressed β-gal. Dashed lines with different colors represent multipeak Gaussian fitting using OriginPro software (Origin Lab): T1, black; T2, red; T3, blue; and T4, pink. Turnover rates of enzymes are calculated by comparing the raw intensity changes with a resorufin calibration curve (SI Appendix, Fig. S8). (B) Peak positions. The peak positions are acquired through Gaussian fitting of the activity histogram in A.

Article Snippet: Dashed lines with different colors represent multipeak Gaussian fitting using OriginPro software (Origin Lab): T1, black; T2, red; T3, blue; and T4, pink.

Techniques: Software, Activity Assay

Journal: Circulation Research

Article Title: TMEM16A/ANO1 Channels Contribute to the Myogenic Response in Cerebral Arteries

doi: 10.1161/circresaha.112.277145

Figure Lengend Snippet: Figure 4. Pressure-induced membrane stretch activates TMEM16A channels in arterial smooth muscle cells. A, Reduction in pipette pressure from 0 to −40 mm Hg stimulates inward currents that are partially inhibited by the TMEM16 A-inhibitory antibody. Traces represent the average of current recordings of 29 control and 18 TMEM16A antibody-exposed patches at −80 mV. B, Exemplary recordings of single channels activated by −40 mm Hg pressure and inhibited by the TMEM16A-inhibitory antibody. C, All-points histograms of arterial smooth muscle cell patches fit with a gaussian function for −40 mm Hg pressure in the absence and presence of the TMEM16A antibody. D, Original cell-attached recordings of recombinant TMEM16A channels expressed in HEK293 cells in the absence and presence of the TMEM16A inhibitory antibody. No patch pressure was applied during experiments on HEK293 cells. E, All-points histograms of recombinant TMEM16A channels fit with a gaussian function in the absence and presence of the TMEM16A antibody.

Article Snippet: All- points histograms were fit with a multipeak gaussian function, using Microcal Origin.

Techniques: Membrane, Transferring, Control, Recombinant