mueller hinton agar  (Thermo Fisher)


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    Name:
    Mueller Hinton Agar
    Description:
    Perform effortless Antimicrobial Susceptibility Testing AST using Thermo Scientific Mueller Hinton Agar with Horse Blood a medium designed and manufactured according to the European standard EUCAST
    Catalog Number:
    pb1229a
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    Applications:
    Blood Culture|Clinical|Clinical Microbiology
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher mueller hinton agar
    Repeatability of Raman spectra of the given organism after growth on different culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; <t>MH—Mueller-Hinton</t> agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640). Plots marked “Without RPMI” and “Without CHROM” are displayed to simplify the comparison of variability of Raman spectra measurement on all of the media. The lower the variability within the species is, the smaller the area covered by an ellipsoid.
    Perform effortless Antimicrobial Susceptibility Testing AST using Thermo Scientific Mueller Hinton Agar with Horse Blood a medium designed and manufactured according to the European standard EUCAST
    https://www.bioz.com/result/mueller hinton agar/product/Thermo Fisher
    Average 99 stars, based on 341 article reviews
    Price from $9.99 to $1999.99
    mueller hinton agar - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy"

    Article Title: Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

    Journal: Sensors (Basel, Switzerland)

    doi: 10.3390/s151129635

    Repeatability of Raman spectra of the given organism after growth on different culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; MH—Mueller-Hinton agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640). Plots marked “Without RPMI” and “Without CHROM” are displayed to simplify the comparison of variability of Raman spectra measurement on all of the media. The lower the variability within the species is, the smaller the area covered by an ellipsoid.
    Figure Legend Snippet: Repeatability of Raman spectra of the given organism after growth on different culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; MH—Mueller-Hinton agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640). Plots marked “Without RPMI” and “Without CHROM” are displayed to simplify the comparison of variability of Raman spectra measurement on all of the media. The lower the variability within the species is, the smaller the area covered by an ellipsoid.

    Techniques Used:

    ( a ) Scores plot of the first two principal components relation for E. coli cultured on two different media—top cluster RPMI (down-triangle), bottom cluster MH (up-triangle). Using two principle components, one can clearly separate the clusters of spectra related to cultivation of the same microorganism on two different media. This suggests that during the growth of bacteria cultivated on RPMI agar, some substances from agar were absorbed into the volume of the colony translating to the final bacterial spectra; ( b ) Plot of loading of PC2 corresponding to Figure 3 a supporting the contribution of spectral bands related to RPMI medium. Because of focusing conditions this is not due to the contribution from the underlying agar; ( c ) Plot of loading of PC2 corresponding to four bacteria included in our study cultured on the Mueller-Hinton agar supporting the contribution of spectral bands related to given microorganisms (three bands of beta-carotene). This is in contrast to Figure 3 b where spectral bands related to RPMI medium were observed and shows that the Mueller-Hinton agar should be the medium of choice for microbiological experiments employing Raman spectroscopy measurements.
    Figure Legend Snippet: ( a ) Scores plot of the first two principal components relation for E. coli cultured on two different media—top cluster RPMI (down-triangle), bottom cluster MH (up-triangle). Using two principle components, one can clearly separate the clusters of spectra related to cultivation of the same microorganism on two different media. This suggests that during the growth of bacteria cultivated on RPMI agar, some substances from agar were absorbed into the volume of the colony translating to the final bacterial spectra; ( b ) Plot of loading of PC2 corresponding to Figure 3 a supporting the contribution of spectral bands related to RPMI medium. Because of focusing conditions this is not due to the contribution from the underlying agar; ( c ) Plot of loading of PC2 corresponding to four bacteria included in our study cultured on the Mueller-Hinton agar supporting the contribution of spectral bands related to given microorganisms (three bands of beta-carotene). This is in contrast to Figure 3 b where spectral bands related to RPMI medium were observed and shows that the Mueller-Hinton agar should be the medium of choice for microbiological experiments employing Raman spectroscopy measurements.

    Techniques Used: Cell Culture, Raman Spectroscopy

    Normalized representative Raman spectra of the microorganisms on all used culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; MH—Mueller-Hinton agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640).
    Figure Legend Snippet: Normalized representative Raman spectra of the microorganisms on all used culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; MH—Mueller-Hinton agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640).

    Techniques Used:

    Raman spectra obtained from different culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; MH—Mueller-Hinton agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640).
    Figure Legend Snippet: Raman spectra obtained from different culture media (ENDO—Endo agar, BA—blood agar; BA-NaCl—blood agar with 10% NaCl; MH—Mueller-Hinton agar; CHROM—CHROMagar Candida; RPMI—Roosvelt-Park Institute Medium 1640).

    Techniques Used:

    2) Product Images from "Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study"

    Article Title: Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study

    Journal: Molecules

    doi: 10.3390/molecules23123266

    The evaluation of minimum inhibitory concentration (MIC) of the hemp EO versus Staphylococcus aureus determined using the alamarBlue ® (AB) assay. ( A ) Representative image of colorimetric MIC determination using AB at 24 h of incubation. The white rectangle indicates the MICs at 8 mg/mL S. aureus 101. ( B ) The plot shows the percentage reduction of AB in the S. aureus broth cultures at different hemp EO concentrations compared to the corresponding untreated samples (0) evaluated as indicated in the experimental section of this manuscript. MHB: Mueller–Hinton broth; EtOH: ethanol. Data are presented as the mean of three replicates of three independent experiments.
    Figure Legend Snippet: The evaluation of minimum inhibitory concentration (MIC) of the hemp EO versus Staphylococcus aureus determined using the alamarBlue ® (AB) assay. ( A ) Representative image of colorimetric MIC determination using AB at 24 h of incubation. The white rectangle indicates the MICs at 8 mg/mL S. aureus 101. ( B ) The plot shows the percentage reduction of AB in the S. aureus broth cultures at different hemp EO concentrations compared to the corresponding untreated samples (0) evaluated as indicated in the experimental section of this manuscript. MHB: Mueller–Hinton broth; EtOH: ethanol. Data are presented as the mean of three replicates of three independent experiments.

    Techniques Used: Concentration Assay, Incubation

    3) Product Images from "Antibiotic resistance patterns and extended-spectrum ?-lactamase production among Acinetobacter spp. isolated from an intensive care Unit of a hospital in Kerman, Iran"

    Article Title: Antibiotic resistance patterns and extended-spectrum ?-lactamase production among Acinetobacter spp. isolated from an intensive care Unit of a hospital in Kerman, Iran

    Journal: Antimicrobial Resistance and Infection Control

    doi: 10.1186/2047-2994-1-1

    MICs of gentamicin [Gm (a)] and amikacin [AK (b)] for Acinetobacter isolates as determined by the E-test . The MIC was measured as lowest concentration of the antibiotic that inhibits the visible growth of the organism. Mueller-Hinton agar with initial CFU/mL 1.5 × 10 7 after 24 hours of incubation at 37°C was used as medium.
    Figure Legend Snippet: MICs of gentamicin [Gm (a)] and amikacin [AK (b)] for Acinetobacter isolates as determined by the E-test . The MIC was measured as lowest concentration of the antibiotic that inhibits the visible growth of the organism. Mueller-Hinton agar with initial CFU/mL 1.5 × 10 7 after 24 hours of incubation at 37°C was used as medium.

    Techniques Used: Concentration Assay, Incubation

    4) Product Images from "Improving Antibiotic Activity against Wound Pathogens with Manuka Honey In Vitro"

    Article Title: Improving Antibiotic Activity against Wound Pathogens with Manuka Honey In Vitro

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0045600

    Effect of antibiotics, manuka honey singly and in combination on the growth of EMRSA-15. EMRSA-15 was cultivated in microtitre plates in Mueller Hinton agar with a range of concentrations of antibiotic with and without 5% (w/v) manuka honey and optical density at 550 nm monitored with time. Only the lowest concentration of antibiotic to allow growth is shown in each experiment. MHB = Mueller Hinton broth; MH = MHB with 5% (w/v) manuka honey; in a) T = tetracycline; in b) I = imipenem; in c) M = mupirocin.
    Figure Legend Snippet: Effect of antibiotics, manuka honey singly and in combination on the growth of EMRSA-15. EMRSA-15 was cultivated in microtitre plates in Mueller Hinton agar with a range of concentrations of antibiotic with and without 5% (w/v) manuka honey and optical density at 550 nm monitored with time. Only the lowest concentration of antibiotic to allow growth is shown in each experiment. MHB = Mueller Hinton broth; MH = MHB with 5% (w/v) manuka honey; in a) T = tetracycline; in b) I = imipenem; in c) M = mupirocin.

    Techniques Used: Concentration Assay

    Antibiotic sensitivity testing of Pseudomonas aeruginosa by disc diffusion. Diameters of zones of inhibition of antibiotics (mm) against P.aeruginosa on plates of Mueller Hinton agar without (grey bars) and with (black bars ) 5% (w/v) manuka honey.
    Figure Legend Snippet: Antibiotic sensitivity testing of Pseudomonas aeruginosa by disc diffusion. Diameters of zones of inhibition of antibiotics (mm) against P.aeruginosa on plates of Mueller Hinton agar without (grey bars) and with (black bars ) 5% (w/v) manuka honey.

    Techniques Used: Diffusion-based Assay, Inhibition

    Sensitivity of Pseudomonas aeruginosa to tetracycline by E strip testing. a) MIC of 32 µg/ml tetracycline was seen on Mueller Hinton agar. b) MIC of 8 µg/ml tetracycline was seen on Mueller Hinton agar containing 5% (w/v) manuka honey.
    Figure Legend Snippet: Sensitivity of Pseudomonas aeruginosa to tetracycline by E strip testing. a) MIC of 32 µg/ml tetracycline was seen on Mueller Hinton agar. b) MIC of 8 µg/ml tetracycline was seen on Mueller Hinton agar containing 5% (w/v) manuka honey.

    Techniques Used: Stripping Membranes

    Antibiotic sensitivity testing of EMRSA-15 by disc diffusion. Diameters of zones of inhibition of antibiotics (mm) against EMRSA-15 on plates of Mueller Hinton agar without (grey bars) and with (black bars) 5% (w/v) manuka honey.
    Figure Legend Snippet: Antibiotic sensitivity testing of EMRSA-15 by disc diffusion. Diameters of zones of inhibition of antibiotics (mm) against EMRSA-15 on plates of Mueller Hinton agar without (grey bars) and with (black bars) 5% (w/v) manuka honey.

    Techniques Used: Diffusion-based Assay, Inhibition

    Sensitivity of EMRSA-15 to imipenem by E strip testing. a) MIC of 4 µg/ml imipenem was seen on Mueller Hinton agar. b) MIC of 0.032 µg/ml imipenem was seen on Mueller Hinton agar containing 5% (w/v) manuka honey.
    Figure Legend Snippet: Sensitivity of EMRSA-15 to imipenem by E strip testing. a) MIC of 4 µg/ml imipenem was seen on Mueller Hinton agar. b) MIC of 0.032 µg/ml imipenem was seen on Mueller Hinton agar containing 5% (w/v) manuka honey.

    Techniques Used: Stripping Membranes

    Effect of antibiotics, manuka honey singly and in combination on the growth of Pseudomonas aeruginosa . P. aeruginosa was cultivated in microtitre plates in Mueller Hinton agar with a range of concentrations of antibiotic with and without 5% (w/v) manuka honey and optical density at 550 nm monitored with time. Only the lowest concentration of antibiotic to allow growth is shown in each experiment. MHB = Mueller Hinton broth; MH = MHB with 5% (w/v) manuka honey; in a) R = rifampicin; in b) T = tetracycline; in c) C = colistin.
    Figure Legend Snippet: Effect of antibiotics, manuka honey singly and in combination on the growth of Pseudomonas aeruginosa . P. aeruginosa was cultivated in microtitre plates in Mueller Hinton agar with a range of concentrations of antibiotic with and without 5% (w/v) manuka honey and optical density at 550 nm monitored with time. Only the lowest concentration of antibiotic to allow growth is shown in each experiment. MHB = Mueller Hinton broth; MH = MHB with 5% (w/v) manuka honey; in a) R = rifampicin; in b) T = tetracycline; in c) C = colistin.

    Techniques Used: Concentration Assay

    5) Product Images from "Virulence characteristics of five new Campylobacter jejuni chicken isolates"

    Article Title: Virulence characteristics of five new Campylobacter jejuni chicken isolates

    Journal: Gut Pathogens

    doi: 10.1186/1757-4749-5-41

    Bile resistance. The ability of C. jejuni chicken isolates (RO14, RO19, RO24, RO29, RO37) and C. jejuni 81-176 to survive to bile salts in Mueller Hinton medium was studied. Following agar growth bacteria were transferred into 50 mL of medium containing 0.5% bile salts. Samples were removed after 24 h and diluted prior plating. The surviving cells were counted after 42 h incubation. The experiments were done in triplicates and Student t test was used for statistical significance.
    Figure Legend Snippet: Bile resistance. The ability of C. jejuni chicken isolates (RO14, RO19, RO24, RO29, RO37) and C. jejuni 81-176 to survive to bile salts in Mueller Hinton medium was studied. Following agar growth bacteria were transferred into 50 mL of medium containing 0.5% bile salts. Samples were removed after 24 h and diluted prior plating. The surviving cells were counted after 42 h incubation. The experiments were done in triplicates and Student t test was used for statistical significance.

    Techniques Used: Incubation

    6) Product Images from "Optimized In Vitro Antibiotic Susceptibility Testing Method for Small-Colony Variant Staphylococcus aureus"

    Article Title: Optimized In Vitro Antibiotic Susceptibility Testing Method for Small-Colony Variant Staphylococcus aureus

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.02330-15

    Quality of SCV growth determined by visual inspection of colony size on agar-based media. Ten SCVs were characterized by colony size as an indicator of adequate growth on various media. *, SCV and NC colony growth was tested at a later date on THM-MHA (which is chemically identical to sup-MHA but which is manufactured by Teknova) and Teknova-manufactured unsupplemented MHA (Teknova-MHA) to confirm that isolates grew comparably on commercially available and in-house-prepared supplemented and unsupplemented MHA. Abbreviations: LB, Luria-Bertani agar; BAP, Trypticase soy agar plus 5% sheep blood; MHA, Mueller-Hinton agar; TSA, Trypticase soy agar; BHI, brain heart infusion agar; SCFMD, synthetic CF sputum agar medium supplemented with herring sperm DNA; sup-, supplementation of base media (MHA, TSA, BHI agar, SCFMD) with SCV growth-supportive nutrients thymidine, hemin, and menadione.
    Figure Legend Snippet: Quality of SCV growth determined by visual inspection of colony size on agar-based media. Ten SCVs were characterized by colony size as an indicator of adequate growth on various media. *, SCV and NC colony growth was tested at a later date on THM-MHA (which is chemically identical to sup-MHA but which is manufactured by Teknova) and Teknova-manufactured unsupplemented MHA (Teknova-MHA) to confirm that isolates grew comparably on commercially available and in-house-prepared supplemented and unsupplemented MHA. Abbreviations: LB, Luria-Bertani agar; BAP, Trypticase soy agar plus 5% sheep blood; MHA, Mueller-Hinton agar; TSA, Trypticase soy agar; BHI, brain heart infusion agar; SCFMD, synthetic CF sputum agar medium supplemented with herring sperm DNA; sup-, supplementation of base media (MHA, TSA, BHI agar, SCFMD) with SCV growth-supportive nutrients thymidine, hemin, and menadione.

    Techniques Used:

    7) Product Images from "Development and Application of an Insertional System for Gene Delivery and Expression in Campylobacter jejuni"

    Article Title: Development and Application of an Insertional System for Gene Delivery and Expression in Campylobacter jejuni

    Journal:

    doi: 10.1128/AEM.71.7.4004-4013.2005

    Restoration of motility after complementation of the maf5 mutation after growth for 3 days on 0.4% Mueller-Hinton agar. 1, 11168H/ maf5 ::Kan r ; 2,11168H/ maf5 ::Kan r /pRMAF; 3, 11168H. A similar picture of restoration of motility was observed after complementation
    Figure Legend Snippet: Restoration of motility after complementation of the maf5 mutation after growth for 3 days on 0.4% Mueller-Hinton agar. 1, 11168H/ maf5 ::Kan r ; 2,11168H/ maf5 ::Kan r /pRMAF; 3, 11168H. A similar picture of restoration of motility was observed after complementation

    Techniques Used: Mutagenesis

    8) Product Images from "Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-Resistant Pseudomonas aeruginosa"

    Article Title: Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-Resistant Pseudomonas aeruginosa

    Journal: bioRxiv

    doi: 10.1101/2020.01.10.902676

    Effect of AgNPs on P. aeruginosa survival. (a) Disc diffusion assay: Paper discs containing each 10 µL of 0, 10, 15, 20, 25 or 30 µg/mL AgNPs were placed on Mueller-Hinton plates spread with bacteria and incubated overnight under dark conditions. (b) Effect of light on the bactericidal effect of AgNPs. Discs containing 10 µg/mL AgNPs were placed over P. aeruginosa lawns in the absence (left side) or presence (right side) of light.
    Figure Legend Snippet: Effect of AgNPs on P. aeruginosa survival. (a) Disc diffusion assay: Paper discs containing each 10 µL of 0, 10, 15, 20, 25 or 30 µg/mL AgNPs were placed on Mueller-Hinton plates spread with bacteria and incubated overnight under dark conditions. (b) Effect of light on the bactericidal effect of AgNPs. Discs containing 10 µg/mL AgNPs were placed over P. aeruginosa lawns in the absence (left side) or presence (right side) of light.

    Techniques Used: Diffusion-based Assay, Incubation

    Effect of light on bacteria growing in Mueller-Hinton medium. Bacterial growth rate was determined by growing cells under dark or light conditions in MH medium and measuring the number of colony forming units (CFU/mL) at different time points.
    Figure Legend Snippet: Effect of light on bacteria growing in Mueller-Hinton medium. Bacterial growth rate was determined by growing cells under dark or light conditions in MH medium and measuring the number of colony forming units (CFU/mL) at different time points.

    Techniques Used:

    9) Product Images from "Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study"

    Article Title: Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study

    Journal: Molecules

    doi: 10.3390/molecules23123266

    The evaluation of minimum inhibitory concentration (MIC) of the hemp EO versus Staphylococcus aureus determined using the alamarBlue ® (AB) assay. ( A ) Representative image of colorimetric MIC determination using AB at 24 h of incubation. The white rectangle indicates the MICs at 8 mg/mL S. aureus 101. ( B ) The plot shows the percentage reduction of AB in the S. aureus broth cultures at different hemp EO concentrations compared to the corresponding untreated samples (0) evaluated as indicated in the experimental section of this manuscript. MHB: Mueller–Hinton broth; EtOH: ethanol. Data are presented as the mean of three replicates of three independent experiments.
    Figure Legend Snippet: The evaluation of minimum inhibitory concentration (MIC) of the hemp EO versus Staphylococcus aureus determined using the alamarBlue ® (AB) assay. ( A ) Representative image of colorimetric MIC determination using AB at 24 h of incubation. The white rectangle indicates the MICs at 8 mg/mL S. aureus 101. ( B ) The plot shows the percentage reduction of AB in the S. aureus broth cultures at different hemp EO concentrations compared to the corresponding untreated samples (0) evaluated as indicated in the experimental section of this manuscript. MHB: Mueller–Hinton broth; EtOH: ethanol. Data are presented as the mean of three replicates of three independent experiments.

    Techniques Used: Concentration Assay, Incubation

    10) Product Images from "Ciprofloxacin-Loaded Keratin Hydrogels Prevent Pseudomonas aeruginosa Infection and Support Healing in a Porcine Full-Thickness Excisional Wound"

    Article Title: Ciprofloxacin-Loaded Keratin Hydrogels Prevent Pseudomonas aeruginosa Infection and Support Healing in a Porcine Full-Thickness Excisional Wound

    Journal: Advances in Wound Care

    doi: 10.1089/wound.2014.0576

    Ciprofloxacin-loaded keratose hydrogels inhibit P. aeruginosa growth in vitro . Mueller-Hinton broth (10 mL) was inoculated with P. aeruginosa , diluted to a concentration of 10 5 colony-forming units (cfu)/mL, and added to 200 μL
    Figure Legend Snippet: Ciprofloxacin-loaded keratose hydrogels inhibit P. aeruginosa growth in vitro . Mueller-Hinton broth (10 mL) was inoculated with P. aeruginosa , diluted to a concentration of 10 5 colony-forming units (cfu)/mL, and added to 200 μL

    Techniques Used: In Vitro, Concentration Assay

    Related Articles

    other:

    Article Title: Antibiotic resistance patterns and extended-spectrum ?-lactamase production among Acinetobacter spp. isolated from an intensive care Unit of a hospital in Kerman, Iran
    Article Snippet: Antibiotic susceptibility tests Antibiotic sensitivity of the isolates was determined using the Kirby-Bauer disk-diffusion breakpoint assay on Mueller-Hinton agar using Oxoid disks (purchased from Hi-Media, India) as recommended previously by the Clinical and Laboratory Standards Institute (CLSI, previously called NCCLS) (2007 guidelines) [ ].

    Cell Culture:

    Article Title: Lipopolysaccharide-Deficient Acinetobacter baumannii Shows Altered Signaling through Host Toll-Like Receptors and Increased Susceptibility to the Host Antimicrobial Peptide LL-37
    Article Snippet: .. A. baumannii strains were maintained on Mueller-Hinton (MH) agar or cultured in cation-adjusted Mueller-Hinton broth (CAMHB; Oxoid) at 37°C with the addition of 10 μg/ml of colistin sulfate (Sigma) for the 19606R strain. ..

    Article Title: Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy
    Article Snippet: .. Before the experiment, the strains were thawed quickly at the room temperature and cultivated on the Mueller-Hinton agar (MH, Oxoid, Basingstoke, UK) at 37 °C for 24 h. Grown colonies were consequently transferred onto certain solid media in Petri dishes: MH, Blood agar with sheep erythrocytes (BA), Blood agar with sheep erythrocytes and 10% NaCl (BA-NaCl), Sabouraud agar (SAB, Merck, Germany), Roosvelt-Park Institute Medium 1640 with L-glutamine (RPMI, Sigma Aldrich, Munich, Germany) and CHROMagar Candida (CHROM, CHROMagar Company, Paris, France), Endo agar (ENDO, Oxoid, Basingstoke, UK), with each organism cultured on four media (see ). ..

    Article Title: Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study
    Article Snippet: .. The samples were cultured on Mueller–Hinton agar (MHA, Oxoid Ltd, Hampshire, UK) and on mannitol salt agar (MSA, Oxoid Ltd, Hampshire, UK). .. The identification of clinical isolates was carried out on the basis of colony morphology, Gram stain, and API® systems (bioMérieux, Marcy l’Etoile, France).

    Article Title: Emergence of High-Level Colistin Resistance in an Acinetobacter baumannii Clinical Isolate Mediated by Inactivation of the Global Regulator H-NS
    Article Snippet: .. All A. baumannii strains were maintained on Mueller-Hinton (MH) agar or cultured in cation-adjusted MH broth (CAMHB; Oxoid) at 37°C, supplemented with 10 μg/ml of colistin sulfate and/or 12.5 μg/ml tetracycline where appropriate. .. Plasmid pWH1266 was obtained from the American Type Culture Collection.

    BIA-KA:

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite
    Article Snippet: .. Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Nitrofurantoin (Nitro; 100 mg) susceptibility test discs were purchased from Becton Dickinson and Company (Franklin Lakes, NJ, USA).

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  • 99
    Thermo Fisher 100 mm mueller hinton agar plates
    Images of <t>Mueller–Hinton</t> agar disc diffusion assays against Escherichia coli at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) <t>100%</t> PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.
    100 Mm Mueller Hinton Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 mm mueller hinton agar plates/product/Thermo Fisher
    Average 99 stars, based on 343 article reviews
    Price from $9.99 to $1999.99
    100 mm mueller hinton agar plates - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher mueller hinton agar plates
    Growth inhibitory effects of L-161,982 are not rescued by exogenous thymidine. S. aureus strain NRS383 was grown in cation-adjusted <t>Mueller-Hinton</t> II broth alone (control), with 50 g/ml of L-161,982 or with 40 g/ml of SXT (positive control) ± 200 µg/ml of thymidine. Cultures were incubated at 37°C non-shaking and aliquots were removed at various time points and cultured for growth (CFU). Results are expressed as % of control at each time point. Mean values from two independent experiments are shown.
    Mueller Hinton Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mueller hinton agar plates/product/Thermo Fisher
    Average 92 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    mueller hinton agar plates - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Images of Mueller–Hinton agar disc diffusion assays against Escherichia coli at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Journal: Medical Devices (Auckland, N.Z.)

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite

    doi: 10.2147/MDER.S146248

    Figure Lengend Snippet: Images of Mueller–Hinton agar disc diffusion assays against Escherichia coli at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Article Snippet: Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Diffusion-based Assay

    Images of Mueller–Hinton agar disc diffusion assay against Staphylococcus aureus at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Journal: Medical Devices (Auckland, N.Z.)

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite

    doi: 10.2147/MDER.S146248

    Figure Lengend Snippet: Images of Mueller–Hinton agar disc diffusion assay against Staphylococcus aureus at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Article Snippet: Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Diffusion-based Assay

    Images of Mueller–Hinton broth assays against Escherichia coli and Staphylococcus aureus at 24 hours. (1) E. coli , (2) S. aureus : (A1) Broth, (B1) control E. coli , (C1) 100% PDMS catheter, (D1) silver-coated catheter, (E1) antibacterial catheter, (F1) PDMS–HNT–PEO–nitrofurantoin, (A2) Broth, (B2) control S. aureus , (C2) 100% PDMS catheter, (D2) silver-coated catheter, (E2) antibacterial catheter, (F2) PDMS–HNT–PEO–nitrofurantoin. Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Journal: Medical Devices (Auckland, N.Z.)

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite

    doi: 10.2147/MDER.S146248

    Figure Lengend Snippet: Images of Mueller–Hinton broth assays against Escherichia coli and Staphylococcus aureus at 24 hours. (1) E. coli , (2) S. aureus : (A1) Broth, (B1) control E. coli , (C1) 100% PDMS catheter, (D1) silver-coated catheter, (E1) antibacterial catheter, (F1) PDMS–HNT–PEO–nitrofurantoin, (A2) Broth, (B2) control S. aureus , (C2) 100% PDMS catheter, (D2) silver-coated catheter, (E2) antibacterial catheter, (F2) PDMS–HNT–PEO–nitrofurantoin. Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Article Snippet: Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques:

    Images of Mueller–Hinton agar disc diffusion assays against Escherichia coli at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Journal: Medical Devices (Auckland, N.Z.)

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite

    doi: 10.2147/MDER.S146248

    Figure Lengend Snippet: Images of Mueller–Hinton agar disc diffusion assays against Escherichia coli at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Article Snippet: Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Diffusion-based Assay

    Images of Mueller–Hinton agar disc diffusion assay against Staphylococcus aureus at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Journal: Medical Devices (Auckland, N.Z.)

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite

    doi: 10.2147/MDER.S146248

    Figure Lengend Snippet: Images of Mueller–Hinton agar disc diffusion assay against Staphylococcus aureus at 24 hours. ( A ) antibacterial catheter, ( B ) silver-coated catheter, ( C ) PDMS–HNT–PEO–nitrofurantoin, ( D ) 100% PDMS catheter, ( E ) PDMS–HNT–PEO, and ( F ) standard nitrofurantoin disc (100 mg). Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Article Snippet: Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Diffusion-based Assay

    Images of Mueller–Hinton broth assays against Escherichia coli and Staphylococcus aureus at 24 hours. (1) E. coli , (2) S. aureus : (A1) Broth, (B1) control E. coli , (C1) 100% PDMS catheter, (D1) silver-coated catheter, (E1) antibacterial catheter, (F1) PDMS–HNT–PEO–nitrofurantoin, (A2) Broth, (B2) control S. aureus , (C2) 100% PDMS catheter, (D2) silver-coated catheter, (E2) antibacterial catheter, (F2) PDMS–HNT–PEO–nitrofurantoin. Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Journal: Medical Devices (Auckland, N.Z.)

    Article Title: Antibacterial and antibiofouling clay nanotube–silicone composite

    doi: 10.2147/MDER.S146248

    Figure Lengend Snippet: Images of Mueller–Hinton broth assays against Escherichia coli and Staphylococcus aureus at 24 hours. (1) E. coli , (2) S. aureus : (A1) Broth, (B1) control E. coli , (C1) 100% PDMS catheter, (D1) silver-coated catheter, (E1) antibacterial catheter, (F1) PDMS–HNT–PEO–nitrofurantoin, (A2) Broth, (B2) control S. aureus , (C2) 100% PDMS catheter, (D2) silver-coated catheter, (E2) antibacterial catheter, (F2) PDMS–HNT–PEO–nitrofurantoin. Abbreviations: HNT, halloysite nanotube; PDMS, polydimethylsiloxane; PEO, polyethylene oxide.

    Article Snippet: Micro BCA Protein Assay Kit, 100-mm Mueller–Hinton agar plates, and Mueller–Hinton liquid broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques:

    Effect of AgNPs on P. aeruginosa survival. (a) Disc diffusion assay: Paper discs containing each 10 µL of 0, 10, 15, 20, 25 or 30 µg/mL AgNPs were placed on Mueller-Hinton plates spread with bacteria and incubated overnight under dark conditions. (b) Effect of light on the bactericidal effect of AgNPs. Discs containing 10 µg/mL AgNPs were placed over P. aeruginosa lawns in the absence (left side) or presence (right side) of light.

    Journal: bioRxiv

    Article Title: Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-Resistant Pseudomonas aeruginosa

    doi: 10.1101/2020.01.10.902676

    Figure Lengend Snippet: Effect of AgNPs on P. aeruginosa survival. (a) Disc diffusion assay: Paper discs containing each 10 µL of 0, 10, 15, 20, 25 or 30 µg/mL AgNPs were placed on Mueller-Hinton plates spread with bacteria and incubated overnight under dark conditions. (b) Effect of light on the bactericidal effect of AgNPs. Discs containing 10 µg/mL AgNPs were placed over P. aeruginosa lawns in the absence (left side) or presence (right side) of light.

    Article Snippet: Mueller-Hinton agar plates were evenly swabbed with 100 µL of a PA14 culture (OD600 = 0.1).

    Techniques: Diffusion-based Assay, Incubation

    Effect of light on bacteria growing in Mueller-Hinton medium. Bacterial growth rate was determined by growing cells under dark or light conditions in MH medium and measuring the number of colony forming units (CFU/mL) at different time points.

    Journal: bioRxiv

    Article Title: Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-Resistant Pseudomonas aeruginosa

    doi: 10.1101/2020.01.10.902676

    Figure Lengend Snippet: Effect of light on bacteria growing in Mueller-Hinton medium. Bacterial growth rate was determined by growing cells under dark or light conditions in MH medium and measuring the number of colony forming units (CFU/mL) at different time points.

    Article Snippet: Mueller-Hinton agar plates were evenly swabbed with 100 µL of a PA14 culture (OD600 = 0.1).

    Techniques:

    Growth inhibitory effects of L-161,982 are not rescued by exogenous thymidine. S. aureus strain NRS383 was grown in cation-adjusted Mueller-Hinton II broth alone (control), with 50 g/ml of L-161,982 or with 40 g/ml of SXT (positive control) ± 200 µg/ml of thymidine. Cultures were incubated at 37°C non-shaking and aliquots were removed at various time points and cultured for growth (CFU). Results are expressed as % of control at each time point. Mean values from two independent experiments are shown.

    Journal: bioRxiv

    Article Title: Mechanism of Action of a Prostaglandin E2 Receptor Antagonist with antimicrobial activity against Staphylococcus aureus

    doi: 10.1101/2020.01.21.914200

    Figure Lengend Snippet: Growth inhibitory effects of L-161,982 are not rescued by exogenous thymidine. S. aureus strain NRS383 was grown in cation-adjusted Mueller-Hinton II broth alone (control), with 50 g/ml of L-161,982 or with 40 g/ml of SXT (positive control) ± 200 µg/ml of thymidine. Cultures were incubated at 37°C non-shaking and aliquots were removed at various time points and cultured for growth (CFU). Results are expressed as % of control at each time point. Mean values from two independent experiments are shown.

    Article Snippet: Hemolysis Assay Hemolytic activity was measured using Mueller-Hinton agar plates containing 5% defribinated sheep blood (Thermo Fisher) +/-L-161,982 at a final concentration of 50 μg/ml.

    Techniques: Positive Control, Incubation, Cell Culture

    L-161,982 treatment inhibits hemolytic activity of S. aureus . A) Macroscopic analysis of S. aureus hemolytic activity. S. aureus was spotted on Mueller-Hinton agar containing sheep blood and/or various concentrations of L-161,982, and incubated at 37C for 24h (0, 25 50, 100) or 72h (200). B) Width of the zone of RBC clearance was measured at 4 points around each S. aureus area of growth in 2 independent replicates. * p

    Journal: bioRxiv

    Article Title: Mechanism of Action of a Prostaglandin E2 Receptor Antagonist with antimicrobial activity against Staphylococcus aureus

    doi: 10.1101/2020.01.21.914200

    Figure Lengend Snippet: L-161,982 treatment inhibits hemolytic activity of S. aureus . A) Macroscopic analysis of S. aureus hemolytic activity. S. aureus was spotted on Mueller-Hinton agar containing sheep blood and/or various concentrations of L-161,982, and incubated at 37C for 24h (0, 25 50, 100) or 72h (200). B) Width of the zone of RBC clearance was measured at 4 points around each S. aureus area of growth in 2 independent replicates. * p

    Article Snippet: Hemolysis Assay Hemolytic activity was measured using Mueller-Hinton agar plates containing 5% defribinated sheep blood (Thermo Fisher) +/-L-161,982 at a final concentration of 50 μg/ml.

    Techniques: Activity Assay, Incubation

    Growth in the presence of L-161,982 inhibits pigment production of S. aureus. Pigment (staphyloxanthin) levels were measured from cultures grown in Mueller-Hinton broth +/-L-161,982 at 37°C for 24h. A) Macroscopic analysis of S. aureus pigment production. B) Pigment was extracted in methanol and optical density measured at 450nm. Data shown are representative of two independent experiments. *** p

    Journal: bioRxiv

    Article Title: Mechanism of Action of a Prostaglandin E2 Receptor Antagonist with antimicrobial activity against Staphylococcus aureus

    doi: 10.1101/2020.01.21.914200

    Figure Lengend Snippet: Growth in the presence of L-161,982 inhibits pigment production of S. aureus. Pigment (staphyloxanthin) levels were measured from cultures grown in Mueller-Hinton broth +/-L-161,982 at 37°C for 24h. A) Macroscopic analysis of S. aureus pigment production. B) Pigment was extracted in methanol and optical density measured at 450nm. Data shown are representative of two independent experiments. *** p

    Article Snippet: Hemolysis Assay Hemolytic activity was measured using Mueller-Hinton agar plates containing 5% defribinated sheep blood (Thermo Fisher) +/-L-161,982 at a final concentration of 50 μg/ml.

    Techniques: