mtbe  (Millipore)

 
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    Name:
    tert Butyl methyl ether
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    Catalog Number:
    50126
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    Structured Review

    Millipore mtbe
    tert Butyl methyl ether

    https://www.bioz.com/result/mtbe/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mtbe - by Bioz Stars, 2021-03
    95/100 stars

    Images

    1) Product Images from "Cometabolism of Methyl tertiary Butyl Ether and Gaseous n-Alkanes by Pseudomonas mendocina KR-1 Grown on C5 to C8n-Alkanes"

    Article Title: Cometabolism of Methyl tertiary Butyl Ether and Gaseous n-Alkanes by Pseudomonas mendocina KR-1 Grown on C5 to C8n-Alkanes

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.69.12.7385-7394.2003

    Effects of cosubstrates on growth yield of cells grown on liquid n -alkanes. The figure shows the effects of gaseous n -alkanes, MTBE, and its metabolites on the final-culture density of P. mendocina KR-1 cells grown under carbon-limited conditions on C 5 to C 8 n -alkanes. A series of cultures of P . mendocina KR-1 were prepared in glass serum vials (125 ml) as described in Materials and Methods. The cultures contained either an n -alkane growth substrate alone (0.02%, vol/vol) (white bars) or (in sequence from left to right) each n -alkane growth substrate (0.02%, vol/vol) with either methane (C 1 ), ethane (C 2 ), propane (C 3 ), n -butane (C 4 ), MTBE (M), formaldehyde (F), or TBA (T) added individually (450 μmol of each compound). The cultures were grown for 5 days, and each culture condition was replicated at a minimum of three times. The figure shows the average final optical density (OD 600 ) for all of the cultures. The error bars show the range of values of the final optical densities.
    Figure Legend Snippet: Effects of cosubstrates on growth yield of cells grown on liquid n -alkanes. The figure shows the effects of gaseous n -alkanes, MTBE, and its metabolites on the final-culture density of P. mendocina KR-1 cells grown under carbon-limited conditions on C 5 to C 8 n -alkanes. A series of cultures of P . mendocina KR-1 were prepared in glass serum vials (125 ml) as described in Materials and Methods. The cultures contained either an n -alkane growth substrate alone (0.02%, vol/vol) (white bars) or (in sequence from left to right) each n -alkane growth substrate (0.02%, vol/vol) with either methane (C 1 ), ethane (C 2 ), propane (C 3 ), n -butane (C 4 ), MTBE (M), formaldehyde (F), or TBA (T) added individually (450 μmol of each compound). The cultures were grown for 5 days, and each culture condition was replicated at a minimum of three times. The figure shows the average final optical density (OD 600 ) for all of the cultures. The error bars show the range of values of the final optical densities.

    Techniques Used: Sequencing

    Time course of MTBE consumption and TBA production by n -pentane-grown cells. n -Pentane-grown cells (total protein, 1.33 mg) were incubated with MTBE (1.1 μmol) in a reaction vial (10 ml), and the changes in reactant concentrations were determined over time by GC, as described in Materials and Methods. The figure shows the time course for MTBE consumption (⧫), TBA production (◊), and the molar balance (MTBE + TBA) (▾) for the reaction. The inset shows the time course of TBA consumption and TBA production from TBF hydrolysis. n -Pentane-grown cells (total protein, 0.3 mg) were incubated with either TBA (1 μmol) or TBF (1 μmol) in a reaction vial (10 ml) as described in Materials and Methods. The changes in reactant and product concentrations were determined over time by GC. The figure shows the time course for TBA consumption for a reaction containing n -pentane-grown cells (▪) and a reaction conducted without cells (buffer alone) (□). The figure also shows the time course for TBF consumption for a reaction conducted with (○) and without (▵) n -pentane-grown cells and the corresponding production of TBA in the presence (•) and absence (▴) of cells.
    Figure Legend Snippet: Time course of MTBE consumption and TBA production by n -pentane-grown cells. n -Pentane-grown cells (total protein, 1.33 mg) were incubated with MTBE (1.1 μmol) in a reaction vial (10 ml), and the changes in reactant concentrations were determined over time by GC, as described in Materials and Methods. The figure shows the time course for MTBE consumption (⧫), TBA production (◊), and the molar balance (MTBE + TBA) (▾) for the reaction. The inset shows the time course of TBA consumption and TBA production from TBF hydrolysis. n -Pentane-grown cells (total protein, 0.3 mg) were incubated with either TBA (1 μmol) or TBF (1 μmol) in a reaction vial (10 ml) as described in Materials and Methods. The changes in reactant and product concentrations were determined over time by GC. The figure shows the time course for TBA consumption for a reaction containing n -pentane-grown cells (▪) and a reaction conducted without cells (buffer alone) (□). The figure also shows the time course for TBF consumption for a reaction conducted with (○) and without (▵) n -pentane-grown cells and the corresponding production of TBA in the presence (•) and absence (▴) of cells.

    Techniques Used: Incubation

    Kinetics of MTBE oxidation by P. mendocina KR-1. The figure shows a plot of the specific rate of TBA production from MTBE versus that from a dissolved MTBE concentration for cells of P . mendocina KR-1 grown on C 5 to C 8 n -alkanes. The reactions were conducted as described in Materials and Methods. The figure shows the average rate of TBA production for cells grown on n -pentane (two different cultures) (▪) and for cells grown on n -hexane, n -heptane, and n -octane (one culture for each n -alkane) (•). The error bars show the range of values around the mean. The plotted curve represents the computer fit to the single-substrate-binding model described in Materials and Methods.
    Figure Legend Snippet: Kinetics of MTBE oxidation by P. mendocina KR-1. The figure shows a plot of the specific rate of TBA production from MTBE versus that from a dissolved MTBE concentration for cells of P . mendocina KR-1 grown on C 5 to C 8 n -alkanes. The reactions were conducted as described in Materials and Methods. The figure shows the average rate of TBA production for cells grown on n -pentane (two different cultures) (▪) and for cells grown on n -hexane, n -heptane, and n -octane (one culture for each n -alkane) (•). The error bars show the range of values around the mean. The plotted curve represents the computer fit to the single-substrate-binding model described in Materials and Methods.

    Techniques Used: Concentration Assay, Binding Assay

    Effects of n -alkanes on MTBE oxidation by n -pentane-grown cells. The figure shows the effects of growth-supporting (A) and non-growth-supporting n -alkanes (B) on the time course of TBA production from MTBE by n -pentane-grown cells. (A) n -Pentane-grown cells (total protein, 0.47 mg) were incubated with MTBE (42 μmol) in a reaction vial (10-ml) as described in Materials and Methods. The figure shows the time course of TBA production from MTBE for cells incubated with MTBE alone (▪) or with MTBE and each of the following n -alkanes (30 μmol): n -pentane (♦), n -hexane (•), n -heptane (▾), and n -octane (▴). (B) The figure shows a plot of the effects of gaseous n -alkanes on TBA production from MTBE by n -pentane-grown cells. Cells (total protein, 1.8 mg) were incubated with MTBE (2.8 μmol) in reaction vials (10 ml) with various concentrations of gaseous n -alkanes as described in Materials and Methods. After being incubated for 25 min, the reaction medium was analyzed by GC to quantify the production of TBA. The figure shows the effects of increasing dissolved concentrations of nitrogen (○), methane (▾), ethane (▴), propane (•), and n -butane (▪) on TBA production, relative to a control reaction conducted without gaseous n -alkanes. The plotted curve represents the computer fit to the single-substrate-binding model described in Materials and Methods.
    Figure Legend Snippet: Effects of n -alkanes on MTBE oxidation by n -pentane-grown cells. The figure shows the effects of growth-supporting (A) and non-growth-supporting n -alkanes (B) on the time course of TBA production from MTBE by n -pentane-grown cells. (A) n -Pentane-grown cells (total protein, 0.47 mg) were incubated with MTBE (42 μmol) in a reaction vial (10-ml) as described in Materials and Methods. The figure shows the time course of TBA production from MTBE for cells incubated with MTBE alone (▪) or with MTBE and each of the following n -alkanes (30 μmol): n -pentane (♦), n -hexane (•), n -heptane (▾), and n -octane (▴). (B) The figure shows a plot of the effects of gaseous n -alkanes on TBA production from MTBE by n -pentane-grown cells. Cells (total protein, 1.8 mg) were incubated with MTBE (2.8 μmol) in reaction vials (10 ml) with various concentrations of gaseous n -alkanes as described in Materials and Methods. After being incubated for 25 min, the reaction medium was analyzed by GC to quantify the production of TBA. The figure shows the effects of increasing dissolved concentrations of nitrogen (○), methane (▾), ethane (▴), propane (•), and n -butane (▪) on TBA production, relative to a control reaction conducted without gaseous n -alkanes. The plotted curve represents the computer fit to the single-substrate-binding model described in Materials and Methods.

    Techniques Used: Incubation, Binding Assay

    2) Product Images from "Induction of Methyl Tertiary Butyl Ether (MTBE)-Oxidizing Activity in Mycobacterium vaccae JOB5 by MTBE"

    Article Title: Induction of Methyl Tertiary Butyl Ether (MTBE)-Oxidizing Activity in Mycobacterium vaccae JOB5 by MTBE

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.2.1023-1030.2004

    Effect of MTBE concentration on MTBE oxidation and production of TBA and TBF. Cultures of M. vaccae JOB5 were grown for 7 days under carbon-limited conditions in batch culture on either glycerol (7.5 mM [▪]) or fructose (2.5 mM [▴]) in
    Figure Legend Snippet: Effect of MTBE concentration on MTBE oxidation and production of TBA and TBF. Cultures of M. vaccae JOB5 were grown for 7 days under carbon-limited conditions in batch culture on either glycerol (7.5 mM [▪]) or fructose (2.5 mM [▴]) in

    Techniques Used: Concentration Assay

    Time course of TBA production by cells of M. vaccae JOB5 during growth on linear organic acids. A series of cultures of M. vaccae JOB5 were grown on linear organic acids (C 2 to C 7 ) (initial concentration = 2.5 mM) in the presence of MTBE (14 μmol),
    Figure Legend Snippet: Time course of TBA production by cells of M. vaccae JOB5 during growth on linear organic acids. A series of cultures of M. vaccae JOB5 were grown on linear organic acids (C 2 to C 7 ) (initial concentration = 2.5 mM) in the presence of MTBE (14 μmol),

    Techniques Used: Concentration Assay

    3) Product Images from "Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma"

    Article Title: Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms17101719

    Effect of solvent used for reconstitution of samples. ( a ) Chromatogram of sample reconstituted in methyl tert -butyl ether (MTBE); ( b ) chromatogram of sample reconstituted in MeOH.
    Figure Legend Snippet: Effect of solvent used for reconstitution of samples. ( a ) Chromatogram of sample reconstituted in methyl tert -butyl ether (MTBE); ( b ) chromatogram of sample reconstituted in MeOH.

    Techniques Used:

    Related Articles

    other:

    Article Title: Conformational Dependence of 13C shielding and coupling constants for methionine methyl groups
    Article Snippet: Ethyl methyl sulfide ((1-methylsulfanyl)ethane), methyl t-butyl sulfide, and methyl t-butyl ether were obtained from Sigma-Aldrich, and [methyl-13 C]-L-methionine was obtained from CIL, Inc. U-[13 C,15 N]ubiquitin was obtained from VLI Research, Inc. (Malvern, PA).

    Article Title: Cometabolism of Methyl tertiary Butyl Ether and Gaseous n-Alkanes by Pseudomonas mendocina KR-1 Grown on C5 to C8n-Alkanes
    Article Snippet: Benzene (≥99% purity), n -butane (99% purity), 1-butanol (99% purity), 2-butanol (99.5% purity), ethylbenzene (99.8% purity), formaldehyde (37% aqueous solution), n -heptane (99% purity), 1-heptanol (98% purity), 2-heptanol (98% purity), n -hexane (≥99% purity), 1-hexanol (≥99% purity), 2-hexanol (99% purity), isobutane (99% purity), isopentane (99% purity), 2-methyl-1-propanol (99.5% purity), 2-methylpentane (99.5% purity), 2-methyl-2-propanol (TBA) (99.3% purity), MTBE (99.8% purity), n -octane (99% purity), 2-octanol (97% purity), 1-pentanol (99% purity), 2-pentanol (98% purity), 1-propanol (99.9% purity), 2-propanol (99.5% purity), toluene (99.8% purity), o -xylene (98% purity), m -xylene (≥99% purity) and p -xylene (≥99% purity) were obtained from Sigma Aldrich Chemical Co. (Milwaukee, Wis.).

    Article Title: Tuning Lignin Characteristics by Fractionation: A Versatile Approach Based on Solvent Extraction and Membrane-Assisted Ultrafiltration
    Article Snippet: Materials All chemicals and analytical grade solvents, such as tetrahydrofuran (THF), methanol (MeOH), ethyl acetate (EtOAc), n-butyl acetate (BuOAc), and tert-butyl methyl ether (MTBE), were purchased from Sigma-Aldrich.

    High Performance Liquid Chromatography:

    Article Title: Widespread Head-to-Head Hydrocarbon Biosynthesis in Bacteria and Role of OleA ▿Widespread Head-to-Head Hydrocarbon Biosynthesis in Bacteria and Role of OleA ▿ †
    Article Snippet: .. Evaporated residue was recovered in 1 ml of methyl- tert -butyl ether (MTBE), applied to a 4.0-g silica gel column (Sigma-Aldrich), and eluted with 35 ml of HPLC-grade hexanes (Fischer Scientific), followed by 35 ml of MTBE and 25 ml of HPLC-grade ethyl acetate (Sigma). .. Each solvent fraction was concentrated and subjected to GC-mass spectrometry (MS) analysis using an HP6890 gas chromatograph connected to an HP5973 mass spectrometer (Hewlett Packard, Palo Alto, CA).

    Article Title: Serial-omics characterization of equine urine
    Article Snippet: .. A 333 μL aliquot of horse urine was taken and 2.475 mL HPLC grade methanol (Pharmco-Aaper, #33900HPLC) was added and vortexed vigorously for 1 min. After the addition of 8.25 mL of 99.8% MTBE (Sigma Aldrich, #306975-1L), the samples were shaken for 1 hr at RT. ..

    Article Title: Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma
    Article Snippet: .. MeOH of LC-MS grade, n -hexane, ethanol and MTBE of HPLC grade, synthetic plasma and butylated hydroxytoluene (BHT) were obtained from Sigma-Aldrich. .. AMAC and AA of HPLC grade were purchased from Panreac Quimica SA (Barcelona, Spain).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma
    Article Snippet: .. MeOH of LC-MS grade, n -hexane, ethanol and MTBE of HPLC grade, synthetic plasma and butylated hydroxytoluene (BHT) were obtained from Sigma-Aldrich. .. AMAC and AA of HPLC grade were purchased from Panreac Quimica SA (Barcelona, Spain).

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    Millipore mbte
    SDS-PAGE (4 to 20% polyacrylamide) and Coomassie staining analysis of purified NRPSs. (A) Purified <t>MbtB</t> (7 μg) overproduced in the absence of MbtH. (B) Purified MbtB, <t>MbtE,</t> and MbtF overproduced in the presence of MbtH. Eleven micrograms of protein
    Mbte, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbte/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mbte - by Bioz Stars, 2021-03
    97/100 stars
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    95
    Millipore tert butyl methyl ether
    SDS-PAGE (4 to 20% polyacrylamide) and Coomassie staining analysis of purified NRPSs. (A) Purified <t>MbtB</t> (7 μg) overproduced in the absence of MbtH. (B) Purified MbtB, <t>MbtE,</t> and MbtF overproduced in the presence of MbtH. Eleven micrograms of protein
    Tert Butyl Methyl Ether, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tert butyl methyl ether/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tert butyl methyl ether - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    SDS-PAGE (4 to 20% polyacrylamide) and Coomassie staining analysis of purified NRPSs. (A) Purified MbtB (7 μg) overproduced in the absence of MbtH. (B) Purified MbtB, MbtE, and MbtF overproduced in the presence of MbtH. Eleven micrograms of protein

    Journal: Journal of Bacteriology

    Article Title: Analyses of MbtB, MbtE, and MbtF Suggest Revisions to the Mycobactin Biosynthesis Pathway in Mycobacterium tuberculosis

    doi: 10.1128/JB.00088-12

    Figure Lengend Snippet: SDS-PAGE (4 to 20% polyacrylamide) and Coomassie staining analysis of purified NRPSs. (A) Purified MbtB (7 μg) overproduced in the absence of MbtH. (B) Purified MbtB, MbtE, and MbtF overproduced in the presence of MbtH. Eleven micrograms of protein

    Article Snippet: The genes coding for MbtB, MbtE, MbtF, and Rv1347c in M. tuberculosis mc2 6020 were cloned into the overexpression vector pET28b (EMD Chemicals) using polymerase incomplete primer extension (PIPE)-based cloning ( ).

    Techniques: SDS Page, Staining, Purification