mt2  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Alomone Labs mt2
    Immunofluorescent colocalization of eNOS and <t>MT2</t> receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm
    Mt2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mt2/product/Alomone Labs
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mt2 - by Bioz Stars, 2022-10
    86/100 stars

    Images

    1) Product Images from "Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin"

    Article Title: Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    doi: 10.1038/s41440-018-0066-9

    Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm
    Figure Legend Snippet: Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm

    Techniques Used: Staining

    Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P
    Figure Legend Snippet: Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P

    Techniques Used: Expressing, Western Blot

    2) Product Images from "Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin"

    Article Title: Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    doi: 10.1038/s41440-018-0066-9

    Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm
    Figure Legend Snippet: Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm

    Techniques Used: Staining

    Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P
    Figure Legend Snippet: Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P

    Techniques Used: Expressing, Western Blot

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Alomone Labs mt2
    Immunofluorescent colocalization of eNOS and <t>MT2</t> receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm
    Mt2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mt2/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mt2 - by Bioz Stars, 2022-10
    90/100 stars
      Buy from Supplier

    90
    Alomone Labs antibody against mt2 receptors
    Immunofluorescent colocalization of eNOS and <t>MT2</t> receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm
    Antibody Against Mt2 Receptors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against mt2 receptors/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against mt2 receptors - by Bioz Stars, 2022-10
    90/100 stars
      Buy from Supplier

    93
    Alomone Labs goat anti mt2
    Temporal pattern of MT 2 mRNA expression and cellular distribution of MT 2 receptor in ciliary processes of C57BL/6J versus DBA/2J mice. A : Total RNA from the ciliary processes of either control (C57BL/6J) or glaucomatous (DBA/2J) animals at 3, 6, 9, or 12 months of age was extracted, and <t>MT2</t> mRNA was quantified with quantitative real-time PCR (qPCR) as described in the Methods section. Values were normalized to the content of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript. Results are the mean ± standard error of the mean (SEM) of 24 animals of each strain (***p
    Goat Anti Mt2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mt2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mt2 - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    Article Title: Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin

    doi: 10.1038/s41440-018-0066-9

    Figure Lengend Snippet: Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm

    Article Snippet: The blot membranes were blocked for 2 h at room temperature and then incubated overnight at 4 °C with rabbit polyclonal antibodies for MT1 and MT2 (1:200, Alomone Laboratories, Jerusalem, Israel) and mouse monoclonal antibodies for eNOS (1:1000, Transduction Laboratories, Lexington, UK).

    Techniques: Staining

    Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    Article Title: Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin

    doi: 10.1038/s41440-018-0066-9

    Figure Lengend Snippet: Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P

    Article Snippet: The blot membranes were blocked for 2 h at room temperature and then incubated overnight at 4 °C with rabbit polyclonal antibodies for MT1 and MT2 (1:200, Alomone Laboratories, Jerusalem, Israel) and mouse monoclonal antibodies for eNOS (1:1000, Transduction Laboratories, Lexington, UK).

    Techniques: Expressing, Western Blot

    Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    Article Title: Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin

    doi: 10.1038/s41440-018-0066-9

    Figure Lengend Snippet: Immunofluorescent colocalization of eNOS and MT2 receptors in MAs. Colocalization of eNOS (red) and MT2 (green) staining is confirmed by the sharp merged image (yellow). Scale bar = 25 μm

    Article Snippet: Paraffin sections (4 μm) were de-paraffined, rehydrated, antigen retrieval was performed, and then permeabilized with 0.3% Triton X-100 for 10 min. After washing with PBS, the sections were blocked for nonspecific antibody binding with 5% bovine serum albumin (BSA) for 1 h. Sections were incubated overnight at 4 °C in 1% BSA containing a primary antibody against MT2 receptors (1:300, Alomone Laboratories, Jerusalem, Israel) and eNOS (1:50, Transduction Laboratories, Lexington, UK) at the same time.

    Techniques: Staining

    Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P

    Journal: Hypertension research : official journal of the Japanese Society of Hypertension

    Article Title: Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin

    doi: 10.1038/s41440-018-0066-9

    Figure Lengend Snippet: Protein expression of MT1, MT2 receptor, and eNOS in mesenteric artery homogenates. A Representative Western immunoblot and densitometric analysis of MT1 and MT2 receptor. B Representative Western blot and densitometric analysis of eNOS. * P

    Article Snippet: Paraffin sections (4 μm) were de-paraffined, rehydrated, antigen retrieval was performed, and then permeabilized with 0.3% Triton X-100 for 10 min. After washing with PBS, the sections were blocked for nonspecific antibody binding with 5% bovine serum albumin (BSA) for 1 h. Sections were incubated overnight at 4 °C in 1% BSA containing a primary antibody against MT2 receptors (1:300, Alomone Laboratories, Jerusalem, Israel) and eNOS (1:50, Transduction Laboratories, Lexington, UK) at the same time.

    Techniques: Expressing, Western Blot

    Temporal pattern of MT 2 mRNA expression and cellular distribution of MT 2 receptor in ciliary processes of C57BL/6J versus DBA/2J mice. A : Total RNA from the ciliary processes of either control (C57BL/6J) or glaucomatous (DBA/2J) animals at 3, 6, 9, or 12 months of age was extracted, and MT2 mRNA was quantified with quantitative real-time PCR (qPCR) as described in the Methods section. Values were normalized to the content of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript. Results are the mean ± standard error of the mean (SEM) of 24 animals of each strain (***p

    Journal: Molecular Vision

    Article Title: Changes in melatonin receptor expression in a murine model of glaucoma

    doi:

    Figure Lengend Snippet: Temporal pattern of MT 2 mRNA expression and cellular distribution of MT 2 receptor in ciliary processes of C57BL/6J versus DBA/2J mice. A : Total RNA from the ciliary processes of either control (C57BL/6J) or glaucomatous (DBA/2J) animals at 3, 6, 9, or 12 months of age was extracted, and MT2 mRNA was quantified with quantitative real-time PCR (qPCR) as described in the Methods section. Values were normalized to the content of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript. Results are the mean ± standard error of the mean (SEM) of 24 animals of each strain (***p

    Article Snippet: Then, the following primary antibodies diluted in PBS containing 0.1% TX-100 were incubated at 4 °C overnight: goat anti-MT1 (Santa Cruz Biotechnologies, Inc. Santa Cruz, CA; sc-15204, 1:100), goat anti-MT2 (Alomone Labs, Jerusalem, Israel; APR-010, 1:100), and goat anti-GPR50 (Santa Cruz Biotechnologies; sc-15215, 1:75).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction

    Effect of melatonin receptor antagonists on IOP reduction mediated by melatonin and 5-MCA-NAT in C57BL/6J (dark bars) versus DBA/2J (light bars) mice at 9 months old. Two intraocular pressure (IOP) measurements were taken before a single dose of either vehicle or 100 μM melatonin ( A ) or 5-MCA-NAT ( B ) was applied, and 3 h later, the IOP was measured again. When indicated, melatonin antagonists, all tested at 100 μM, were instilled 30 min before the agonist. Luzindole is a non-selective melatonin antagonist; 4-phenyl-2- propionamidotetralin (4PPDOT) is a MT2 melatonin antagonist, prazosin is a MT3 melatonin antagonist/alpha blocker, and corynanthine is an alpha blocker. Data are the mean ± standard error of the mean (SEM) of four mice (*p

    Journal: Molecular Vision

    Article Title: Changes in melatonin receptor expression in a murine model of glaucoma

    doi:

    Figure Lengend Snippet: Effect of melatonin receptor antagonists on IOP reduction mediated by melatonin and 5-MCA-NAT in C57BL/6J (dark bars) versus DBA/2J (light bars) mice at 9 months old. Two intraocular pressure (IOP) measurements were taken before a single dose of either vehicle or 100 μM melatonin ( A ) or 5-MCA-NAT ( B ) was applied, and 3 h later, the IOP was measured again. When indicated, melatonin antagonists, all tested at 100 μM, were instilled 30 min before the agonist. Luzindole is a non-selective melatonin antagonist; 4-phenyl-2- propionamidotetralin (4PPDOT) is a MT2 melatonin antagonist, prazosin is a MT3 melatonin antagonist/alpha blocker, and corynanthine is an alpha blocker. Data are the mean ± standard error of the mean (SEM) of four mice (*p

    Article Snippet: Then, the following primary antibodies diluted in PBS containing 0.1% TX-100 were incubated at 4 °C overnight: goat anti-MT1 (Santa Cruz Biotechnologies, Inc. Santa Cruz, CA; sc-15204, 1:100), goat anti-MT2 (Alomone Labs, Jerusalem, Israel; APR-010, 1:100), and goat anti-GPR50 (Santa Cruz Biotechnologies; sc-15215, 1:75).

    Techniques: Mouse Assay