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Caltag-Medsystems msu crystals
<t>IL-1β</t> secretion by PBMCs following exposure to <t>MSU</t> crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p
Msu Crystals, supplied by Caltag-Medsystems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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1) Product Images from "Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing"

Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing

Journal: Particle and Fibre Toxicology

doi: 10.1186/s12989-017-0232-2

IL-1β secretion by PBMCs following exposure to MSU crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p
Figure Legend Snippet: IL-1β secretion by PBMCs following exposure to MSU crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p

Techniques Used: Incubation

2) Product Images from "Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing"

Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing

Journal: Particle and Fibre Toxicology

doi: 10.1186/s12989-017-0232-2

IL-1β secretion by PBMCs following exposure to MSU crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p
Figure Legend Snippet: IL-1β secretion by PBMCs following exposure to MSU crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p

Techniques Used: Incubation

Related Articles

other:

Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing
Article Snippet: Finally, paired T tests were used to compare supernatant levels of IL-1β for cells exposed to MSU crystals or SNPs versus non-particle-exposed control cells.

Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing
Article Snippet: For example, MSU crystals and silica particles are activators of the inflammasome (as exemplified here (Fig. ) and [ , , ]) and have direct relevance in terms of human exposure.

Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing
Article Snippet: MSU crystals may precipitate ectopically and are the cause of joint inflammation in patients with gout, whilst silica exposure to the lungs is well established as an occupational hazard that leads to silicosis in miners.

Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing
Article Snippet: SNPs and MSU crystals are considered exemplar particulate stimulants of the inflammasome, and we confirmed that, with similar short exposures as for our TiO2 particles (3 h) and LPS priming, IL-1β secretion was enhanced compared to non-particle-exposed cells (Fig. ).

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    Caltag-Medsystems msu crystals
    <t>IL-1β</t> secretion by PBMCs following exposure to <t>MSU</t> crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p
    Msu Crystals, supplied by Caltag-Medsystems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msu crystals/product/Caltag-Medsystems
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    msu crystals - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    IL-1β secretion by PBMCs following exposure to MSU crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p

    Journal: Particle and Fibre Toxicology

    Article Title: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processing

    doi: 10.1186/s12989-017-0232-2

    Figure Lengend Snippet: IL-1β secretion by PBMCs following exposure to MSU crystals or SNPs. Secretion of IL-1β by PBMCs with (main figure) or without (inset) initial exposure to 10 ng/mL LPS for 3 h followed by exposure to MSU crystals (100 μg/mL) or SNPs (100 μg/mL) for a further 3 h. The supernatant was either collected immediately for analysis (3 h; open bars) or following a further 21 h of cell incubation in fresh (i.e. without added particles or MAMPs) TCM (3 + 21 h; black bars). All data are expressed as mean ± SEM ( n = 4). Results were analysed by paired T test in comparison to baseline (B), i.e. non-particle-exposed cells; * p

    Article Snippet: Finally, paired T tests were used to compare supernatant levels of IL-1β for cells exposed to MSU crystals or SNPs versus non-particle-exposed control cells.

    Techniques: Incubation