Structured Review

Roche mspi
Restriction endonuclease digests of the 16S rRNA genes from Ruminococcus sp. strain YE71 that were not cut (lane 5) or cut with <t>CfoI</t> (lane 2), HaeIII (lane 6), or <t>MspI</t> (lane 10); rRNA genes from R. albus AR67 that were not cut (lane 9) or cut with CfoI
Mspi, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mspi/product/Roche
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
mspi - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "Naturally Occurring DNA Transfer System Associated with Membrane Vesicles in Cellulolytic Ruminococcus spp. of Ruminal Origin"

Article Title: Naturally Occurring DNA Transfer System Associated with Membrane Vesicles in Cellulolytic Ruminococcus spp. of Ruminal Origin

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.71.8.4248-4253.2005

Restriction endonuclease digests of the 16S rRNA genes from Ruminococcus sp. strain YE71 that were not cut (lane 5) or cut with CfoI (lane 2), HaeIII (lane 6), or MspI (lane 10); rRNA genes from R. albus AR67 that were not cut (lane 9) or cut with CfoI
Figure Legend Snippet: Restriction endonuclease digests of the 16S rRNA genes from Ruminococcus sp. strain YE71 that were not cut (lane 5) or cut with CfoI (lane 2), HaeIII (lane 6), or MspI (lane 10); rRNA genes from R. albus AR67 that were not cut (lane 9) or cut with CfoI

Techniques Used:

Related Articles

Clone Assay:

Article Title: SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2
Article Snippet: .. Each FISH probe was made from multiple BAC clones spanning ∼200–350 Kb: 15 µg of BAC DNA was digested with AluI, Rsa, MseI, MspI, HaeIII, and BfuCl overnight at 37°C, ethanol precipitated, and resuspended in ddH2 O. DNA was denatured at 100°C for 1 min, and then 3′-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche). ..

Amplification:

Article Title: In Vitro Susceptibility and Trailing Growth Effect of Clinical Isolates of Candida Species to Azole Drugs
Article Snippet: .. The amplified target sequence was digested with MspI (Roche Molecular Biochemicals, Mannheim, Germany) restriction endonuclease to yield the best species-specific pattern ( ). .. Moreover, C. dubliniensis was differentiated from C. albicans using additional enzyme. (B1nI (AvrII)) (Roche Molecular Biochemicals, Mannheim, Germany) ( ).

Article Title: por Variable-Region Typing by DNA Probe Hybridization Is Broadly Applicable to Epidemiologic Studies of Neisseria gonorrhoeae
Article Snippet: .. Amplified DNA fragments were digested with restriction endonucleases AluI, HaeIII, RsaI, and MspI (Roche Molecular Biochemicals) by using 8 μl of PCR product, 1.0 μl of 10× restriction endonuclease buffer, 10 U of restriction endonuclease, and sterile distilled water to a final volume of 10 μl. .. The mixture was incubated at 37°C for 1 h and then separated on 1.5% agarose gel and stained with ethidium bromide.

Agarose Gel Electrophoresis:

Article Title: Impact of Bt Corn on Rhizospheric and Soil Eubacterial Communities and on Beneficial Mycorrhizal Symbiosis in Experimental Microcosms
Article Snippet: .. An aliquot of each PCR, containing 200 ng of DNA, was digested with 10 U of the restriction enzymes AluI and MspI (Roche Diagnostics) separately in a total volume of 20 μl at 37°C for 3 h. The reaction products were analyzed on a 1× Tris-borate-EDTA agarose gel (2.5% [wt/vol]) with an electrophoretic run at 150 V-150 mA for 150 min. ..

BAC Assay:

Article Title: SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2
Article Snippet: .. Each FISH probe was made from multiple BAC clones spanning ∼200–350 Kb: 15 µg of BAC DNA was digested with AluI, Rsa, MseI, MspI, HaeIII, and BfuCl overnight at 37°C, ethanol precipitated, and resuspended in ddH2 O. DNA was denatured at 100°C for 1 min, and then 3′-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche). ..

Isolation:

Article Title: Naturally Occurring DNA Transfer System Associated with Membrane Vesicles in Cellulolytic Ruminococcus spp. of Ruminal Origin
Article Snippet: .. The conditions used for digestion of isolated nucleic acids by DNase I and RNase A (Roche, Mannheim, Germany) and with restriction endonucleases HindIII and EcoRI and for digestion of PCR products with CfoI, MspI, and HaeIII were the conditions specified by the manufacturer (Roche). ..

Purification:

Article Title: The Distribution and Identity of Edaphic Fungi in the McMurdo Dry Valleys
Article Snippet: .. 40 ng of DNA was digested with 2 U of MspI and 1× restriction enzyme buffer (Roche Applied Science, Branford, CT, USA) according to manufacturer instructions and purified with Ultraclean 15 DNA Purification kit. .. Lengths of fluorescent-labeled PCR amplicons (i.e. , tRFLP fragments) were determined by capillary electrophoresis at the Waikato DNA Sequencing Facility using an ABI 3130 Genetic Analyzer (Life Technologies, Carlsbad, CA, USA) at 10 kV, a separation temperature of 44 °C for 2 h, and the GeneScan 1200 LIZ dye Size Standard (Life Technologies, Carlsbad, CA, USA).

Sequencing:

Article Title: In Vitro Susceptibility and Trailing Growth Effect of Clinical Isolates of Candida Species to Azole Drugs
Article Snippet: .. The amplified target sequence was digested with MspI (Roche Molecular Biochemicals, Mannheim, Germany) restriction endonuclease to yield the best species-specific pattern ( ). .. Moreover, C. dubliniensis was differentiated from C. albicans using additional enzyme. (B1nI (AvrII)) (Roche Molecular Biochemicals, Mannheim, Germany) ( ).

DNA Purification:

Article Title: The Distribution and Identity of Edaphic Fungi in the McMurdo Dry Valleys
Article Snippet: .. 40 ng of DNA was digested with 2 U of MspI and 1× restriction enzyme buffer (Roche Applied Science, Branford, CT, USA) according to manufacturer instructions and purified with Ultraclean 15 DNA Purification kit. .. Lengths of fluorescent-labeled PCR amplicons (i.e. , tRFLP fragments) were determined by capillary electrophoresis at the Waikato DNA Sequencing Facility using an ABI 3130 Genetic Analyzer (Life Technologies, Carlsbad, CA, USA) at 10 kV, a separation temperature of 44 °C for 2 h, and the GeneScan 1200 LIZ dye Size Standard (Life Technologies, Carlsbad, CA, USA).

Polymerase Chain Reaction:

Article Title: por Variable-Region Typing by DNA Probe Hybridization Is Broadly Applicable to Epidemiologic Studies of Neisseria gonorrhoeae
Article Snippet: .. Amplified DNA fragments were digested with restriction endonucleases AluI, HaeIII, RsaI, and MspI (Roche Molecular Biochemicals) by using 8 μl of PCR product, 1.0 μl of 10× restriction endonuclease buffer, 10 U of restriction endonuclease, and sterile distilled water to a final volume of 10 μl. .. The mixture was incubated at 37°C for 1 h and then separated on 1.5% agarose gel and stained with ethidium bromide.

Article Title: Impact of Bt Corn on Rhizospheric and Soil Eubacterial Communities and on Beneficial Mycorrhizal Symbiosis in Experimental Microcosms
Article Snippet: .. An aliquot of each PCR, containing 200 ng of DNA, was digested with 10 U of the restriction enzymes AluI and MspI (Roche Diagnostics) separately in a total volume of 20 μl at 37°C for 3 h. The reaction products were analyzed on a 1× Tris-borate-EDTA agarose gel (2.5% [wt/vol]) with an electrophoretic run at 150 V-150 mA for 150 min. ..

Article Title: Naturally Occurring DNA Transfer System Associated with Membrane Vesicles in Cellulolytic Ruminococcus spp. of Ruminal Origin
Article Snippet: .. The conditions used for digestion of isolated nucleic acids by DNase I and RNase A (Roche, Mannheim, Germany) and with restriction endonucleases HindIII and EcoRI and for digestion of PCR products with CfoI, MspI, and HaeIII were the conditions specified by the manufacturer (Roche). ..

Fluorescence In Situ Hybridization:

Article Title: SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2
Article Snippet: .. Each FISH probe was made from multiple BAC clones spanning ∼200–350 Kb: 15 µg of BAC DNA was digested with AluI, Rsa, MseI, MspI, HaeIII, and BfuCl overnight at 37°C, ethanol precipitated, and resuspended in ddH2 O. DNA was denatured at 100°C for 1 min, and then 3′-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche). ..

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    Roche mspi
    Restriction endonuclease digests of the 16S rRNA genes from Ruminococcus sp. strain YE71 that were not cut (lane 5) or cut with <t>CfoI</t> (lane 2), HaeIII (lane 6), or <t>MspI</t> (lane 10); rRNA genes from R. albus AR67 that were not cut (lane 9) or cut with CfoI
    Mspi, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/Roche
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Restriction endonuclease digests of the 16S rRNA genes from Ruminococcus sp. strain YE71 that were not cut (lane 5) or cut with CfoI (lane 2), HaeIII (lane 6), or MspI (lane 10); rRNA genes from R. albus AR67 that were not cut (lane 9) or cut with CfoI

    Journal: Applied and Environmental Microbiology

    Article Title: Naturally Occurring DNA Transfer System Associated with Membrane Vesicles in Cellulolytic Ruminococcus spp. of Ruminal Origin

    doi: 10.1128/AEM.71.8.4248-4253.2005

    Figure Lengend Snippet: Restriction endonuclease digests of the 16S rRNA genes from Ruminococcus sp. strain YE71 that were not cut (lane 5) or cut with CfoI (lane 2), HaeIII (lane 6), or MspI (lane 10); rRNA genes from R. albus AR67 that were not cut (lane 9) or cut with CfoI

    Article Snippet: The conditions used for digestion of isolated nucleic acids by DNase I and RNase A (Roche, Mannheim, Germany) and with restriction endonucleases HindIII and EcoRI and for digestion of PCR products with CfoI, MspI, and HaeIII were the conditions specified by the manufacturer (Roche).

    Techniques: