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New England Biolabs mse i
Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mse i/product/New England Biolabs
Average 99 stars, based on 74 article reviews
Price from $9.99 to $1999.99
mse i - by Bioz Stars, 2020-01
99/100 stars

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Polymerase Chain Reaction:

Article Title: AFLP assessment of genetic diversity among Indian Mucuna accessions
Article Snippet: AFLP fingerprinting was carried out as described by Capo-chichi et al. ( ) with some modifications as follows: Genomic DNA (500 ng) was digested with 10 U of Eco RI and 4 U of Mse I (New England Biolabs, USA) at 37 °C for 3 h. Without inactivating the restriction enzymes, adapters [ Eco RI (5 pmol) and Mse I (50 pmol)] were ligated to the restricted DNA fragments in ligation buffer [1x T4 DNA ligase buffer, 1 μl of T4 DNA ligase (New England Biolabs, USA)] and incubated at 37 °C for 12 h. Preamplification of the diluted (2-fold), ligated DNA was carried out with primers complimentary to the Eco RI and Mse I adapters, with two sets of selective nucleotides, one with cytosine and guanine and the other with adenine and cytosine respectively in PTC-200™ (MJ Research Inc., USA) thermocycler using the following cycling parameters: 20 cycles of 94 °C (denaturation) for 30 s, 56 °C (annealing) for 60 s, 72 °C (extension) for 60 s. The diluted (4-fold), amplified products were then used as the template for selective amplification. .. The PCR program consisted of two segments: The first segment comprised of 12 cycles with one cycle at 94 °C for 30 s, 65 °C for 30 s, and 72 °C for 60 s. The annealing temperature was then lowered by 0.7 °C per cycle during the first 12 cycles to reach an optimum temperature of 56 °C.

Article Title: Validation of an in-house real-time PCR fecal assay and comparison with two commercial assays for the antemortem detection of Mycobacterium avium subsp. paratuberculosis infection in culled sheep
Article Snippet: PCR amplifications were performed under the following conditions: 1 cycle of 3 min at 94°C and 35 cycles of 30 s at 94°C, 15 s at 62°C, and 1 min at 72°C. .. Digestion of the amplicons using restriction enzymes was carried out in a volume of 30 µL, containing 20 µL of IS 1311 amplicons, 3 µL of reaction 10× buffer, and 10 U of each endonucleases Hin fI and Mse I (New England Biolabs, Beverly, MA).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Paragraph title: Group typing of Map strains using IS1311 PCR-REA ... Digestion of the amplicons using restriction enzymes (REA reactions) were carried out in a volume of 30 μL, containing 20 μL of IS 1311 amplicons, 3 μL of reaction 10× buffer, and 10 U of each endonucleases Hinf I and Mse I (New England Biolabs, Whitby, Ontario).

Electrophoresis:

Article Title: Validation of an in-house real-time PCR fecal assay and comparison with two commercial assays for the antemortem detection of Mycobacterium avium subsp. paratuberculosis infection in culled sheep
Article Snippet: Digestion of the amplicons using restriction enzymes was carried out in a volume of 30 µL, containing 20 µL of IS 1311 amplicons, 3 µL of reaction 10× buffer, and 10 U of each endonucleases Hin fI and Mse I (New England Biolabs, Beverly, MA). .. Reactions were incubated at 37°C for 2.5 h. Restriction reactions were analyzed using a capillary electrophoresis instrument (QIAxcel, Qiagen).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Amplification reactions were analyzed on a capillary electrophoresis instrument (Qiaxcel; Qiagen). .. Digestion of the amplicons using restriction enzymes (REA reactions) were carried out in a volume of 30 μL, containing 20 μL of IS 1311 amplicons, 3 μL of reaction 10× buffer, and 10 U of each endonucleases Hinf I and Mse I (New England Biolabs, Whitby, Ontario).

Incubation:

Article Title: AFLP assessment of genetic diversity among Indian Mucuna accessions
Article Snippet: .. AFLP fingerprinting was carried out as described by Capo-chichi et al. ( ) with some modifications as follows: Genomic DNA (500 ng) was digested with 10 U of Eco RI and 4 U of Mse I (New England Biolabs, USA) at 37 °C for 3 h. Without inactivating the restriction enzymes, adapters [ Eco RI (5 pmol) and Mse I (50 pmol)] were ligated to the restricted DNA fragments in ligation buffer [1x T4 DNA ligase buffer, 1 μl of T4 DNA ligase (New England Biolabs, USA)] and incubated at 37 °C for 12 h. Preamplification of the diluted (2-fold), ligated DNA was carried out with primers complimentary to the Eco RI and Mse I adapters, with two sets of selective nucleotides, one with cytosine and guanine and the other with adenine and cytosine respectively in PTC-200™ (MJ Research Inc., USA) thermocycler using the following cycling parameters: 20 cycles of 94 °C (denaturation) for 30 s, 56 °C (annealing) for 60 s, 72 °C (extension) for 60 s. The diluted (4-fold), amplified products were then used as the template for selective amplification. .. The second amplification was carried out with twelve selective primer combinations of Eco RI and Mse I each with three selective nucleotides (Table ) in a total volume of 10 μl.

Article Title: Validation of an in-house real-time PCR fecal assay and comparison with two commercial assays for the antemortem detection of Mycobacterium avium subsp. paratuberculosis infection in culled sheep
Article Snippet: Digestion of the amplicons using restriction enzymes was carried out in a volume of 30 µL, containing 20 µL of IS 1311 amplicons, 3 µL of reaction 10× buffer, and 10 U of each endonucleases Hin fI and Mse I (New England Biolabs, Beverly, MA). .. Reactions were incubated at 37°C for 2.5 h. Restriction reactions were analyzed using a capillary electrophoresis instrument (QIAxcel, Qiagen).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Digestion of the amplicons using restriction enzymes (REA reactions) were carried out in a volume of 30 μL, containing 20 μL of IS 1311 amplicons, 3 μL of reaction 10× buffer, and 10 U of each endonucleases Hinf I and Mse I (New England Biolabs, Whitby, Ontario). .. Reactions were incubated at 37°C for 2.5 h. Restriction reactions were analyzed using a capillary electrophoresis instrument (Qiaxcel; Qiagen).

Amplification:

Article Title: AFLP assessment of genetic diversity among Indian Mucuna accessions
Article Snippet: .. AFLP fingerprinting was carried out as described by Capo-chichi et al. ( ) with some modifications as follows: Genomic DNA (500 ng) was digested with 10 U of Eco RI and 4 U of Mse I (New England Biolabs, USA) at 37 °C for 3 h. Without inactivating the restriction enzymes, adapters [ Eco RI (5 pmol) and Mse I (50 pmol)] were ligated to the restricted DNA fragments in ligation buffer [1x T4 DNA ligase buffer, 1 μl of T4 DNA ligase (New England Biolabs, USA)] and incubated at 37 °C for 12 h. Preamplification of the diluted (2-fold), ligated DNA was carried out with primers complimentary to the Eco RI and Mse I adapters, with two sets of selective nucleotides, one with cytosine and guanine and the other with adenine and cytosine respectively in PTC-200™ (MJ Research Inc., USA) thermocycler using the following cycling parameters: 20 cycles of 94 °C (denaturation) for 30 s, 56 °C (annealing) for 60 s, 72 °C (extension) for 60 s. The diluted (4-fold), amplified products were then used as the template for selective amplification. .. The second amplification was carried out with twelve selective primer combinations of Eco RI and Mse I each with three selective nucleotides (Table ) in a total volume of 10 μl.

Article Title: Validation of an in-house real-time PCR fecal assay and comparison with two commercial assays for the antemortem detection of Mycobacterium avium subsp. paratuberculosis infection in culled sheep
Article Snippet: The expected amplicon size was 608 nucleotides. .. Digestion of the amplicons using restriction enzymes was carried out in a volume of 30 µL, containing 20 µL of IS 1311 amplicons, 3 µL of reaction 10× buffer, and 10 U of each endonucleases Hin fI and Mse I (New England Biolabs, Beverly, MA).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: The expected amplicon size was 608 nucleotides. .. Digestion of the amplicons using restriction enzymes (REA reactions) were carried out in a volume of 30 μL, containing 20 μL of IS 1311 amplicons, 3 μL of reaction 10× buffer, and 10 U of each endonucleases Hinf I and Mse I (New England Biolabs, Whitby, Ontario).

Ligation:

Article Title: AFLP assessment of genetic diversity among Indian Mucuna accessions
Article Snippet: .. AFLP fingerprinting was carried out as described by Capo-chichi et al. ( ) with some modifications as follows: Genomic DNA (500 ng) was digested with 10 U of Eco RI and 4 U of Mse I (New England Biolabs, USA) at 37 °C for 3 h. Without inactivating the restriction enzymes, adapters [ Eco RI (5 pmol) and Mse I (50 pmol)] were ligated to the restricted DNA fragments in ligation buffer [1x T4 DNA ligase buffer, 1 μl of T4 DNA ligase (New England Biolabs, USA)] and incubated at 37 °C for 12 h. Preamplification of the diluted (2-fold), ligated DNA was carried out with primers complimentary to the Eco RI and Mse I adapters, with two sets of selective nucleotides, one with cytosine and guanine and the other with adenine and cytosine respectively in PTC-200™ (MJ Research Inc., USA) thermocycler using the following cycling parameters: 20 cycles of 94 °C (denaturation) for 30 s, 56 °C (annealing) for 60 s, 72 °C (extension) for 60 s. The diluted (4-fold), amplified products were then used as the template for selective amplification. .. The second amplification was carried out with twelve selective primer combinations of Eco RI and Mse I each with three selective nucleotides (Table ) in a total volume of 10 μl.

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    New England Biolabs mse i
    Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mse i/product/New England Biolabs
    Average 90 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    mse i - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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